Method for extracting active peptides from Qinzhou yellow pond turtle

A technology of Qinzhou stone and active peptide, which is applied in the field of active peptide preparation, can solve the problems of low yield and long time, and achieve the effects of high purification rate and separation degree, increased extraction rate and shortened time

Active Publication Date: 2015-02-25
YANGXIN XINRUI DYEING & WEAVING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The fermentation broth was cultured with intermittent stirring at 15-40°C for 1-4 days, then the fermentation broth was broken in a boiling water bath for 10-40 minutes, the supernatant was collected by centrifugation, concentrated and dried to obtain skin longevity factor peptide with high protein content and good functional activity , but the time is long and the yield is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A method for extracting active peptide from Qinzhou stone money turtle as raw material, comprising the steps of:

[0022] a. Raw material processing: take the Qinzhou stone money turtle, remove the viscera, clean it, and keep the shell and meat;

[0023] b. Hydrolysis: add 8 times the amount of pure water, adjust the pH value of the mixture to 2.5 with 1M HCl, and maintain the pressure of 0.1Mpa after boiling for 10 hours. During the process of boiling and maintaining the pressure, pay attention to replenish the lost water;

[0024] c. Enzymolysis: After the hydrolysis is completed, filter, collect the filtrate, add 1M NaOH to adjust the pH to 5.5, add 0.3% by weight of papain, and perform enzymolysis at 50°C for 12 hours;

[0025] d. Freezing: centrifuge the solution after enzymatic hydrolysis for 30 minutes at a speed of 4000r / min, collect the precipitate, and freeze at -10°C for 24 hours;

[0026] e. Melting and concentrating: take out the frozen material obtained i...

Embodiment 2

[0029] A method for extracting active peptide from Qinzhou stone money turtle as raw material, comprising the steps of:

[0030] a. Raw material processing: take the Qinzhou stone money turtle, remove the viscera, clean it, and keep the shell and meat;

[0031] b. Hydrolysis: Add 10 times the amount of pure water, adjust the pH value of the mixture to 1.8 with 1M HCl, and maintain the pressure of 0.1Mpa after boiling for 8 hours for hydrolysis. During the process of boiling and maintaining the pressure, pay attention to replenish the lost water;

[0032] c. Enzymolysis: After the hydrolysis is completed, filter, collect the filtrate, add 1M NaOH to adjust the pH value to 3.5, add 0.2% by weight of papain, and perform enzymolysis at 45°C for 10 hours;

[0033] d. Freezing: centrifuge the solution after enzymatic hydrolysis for 30 minutes at a speed of 4000r / min, collect the precipitate, and freeze at -10°C for 24 hours;

[0034] e. Melting and concentrating: take out the froze...

Embodiment 3

[0037] A method for extracting active peptide from Qinzhou stone money turtle as raw material, comprising the steps of:

[0038] a. Raw material processing: take the Qinzhou stone money turtle, remove the viscera, clean it, and keep the shell and meat;

[0039] b. Hydrolysis: Add 10 times the amount of pure water, adjust the pH of the mixture to 2.0 with 1M HCl, and maintain the pressure at 0.1Mpa for 10 hours after boiling, and replenish the lost water during the boiling process;

[0040] c. Enzymolysis: After the hydrolysis is completed, filter and collect the filtrate, add 1M NaOH to adjust the pH to 4.5, add 0.25% by weight of papain, and perform enzymolysis at 50°C for 12 hours;

[0041] d. Freezing: centrifuge the solution after enzymatic hydrolysis for 30 minutes at a speed of 4000r / min, collect the precipitate, and freeze at -10°C for 24 hours;

[0042] e. Melting and concentrating: take out the frozen material obtained in the previous step, heat it to melt, filter, c...

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PUM

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Abstract

The invention discloses a method for extracting active peptides from a Qinzhou yellow pond turtle. The method comprises the following steps of raw material treatment, hydrolysis, enzymolysis, freezing, melting, concentrating and spray drying to obtain a finished product. Compared with the traditional seed solution shaking table activation, inoculation, fermentation liquid culture and wall breaking technologies, the method has the advantage that the time is greatly shortened.

Description

technical field [0001] The invention relates to a preparation method of an active peptide, in particular to a method for extracting the active peptide from Qinzhou stone money turtle. Background technique [0002] Stone money turtle, also known as stone turtle, belongs to the genus Pseudowater turtle, which belongs to amphibious animals, and has extremely high medicinal value, edible value, ornamental value and humanistic value. Everyone knows that turtles are long-lived. Turtles have a lifespan of hundreds of years. As the saying goes, "thousand-year bastards, ten-thousand-year turtles", tortoises not only have strong vitality, but also have polypeptide longevity factors in their bodies. Edible and medicinal turtle meat can prolong life, and the polypeptide longevity factor in the longevity turtle can be fully absorbed by the human body. Many active substances in the human body exist in the form of peptides. It has been found that more than 70% of the dry weight of the hu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06
Inventor 归三岷
Owner YANGXIN XINRUI DYEING & WEAVING
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