The invention discloses a nano-scale collagen purification method capable of reaching the absorption rate of 96%. The method includes the steps: crushing fish skins, and removing impurities; placing crushed materials into a low-temperature stirrer, stirring and mixing mixture for 30 minutes according to the volume ratio of the crushed materials to deionized water, standing for 10 minutes, removingupper floats, and placing mixture into a filter device to perform adsorption filtration; collecting filtered crushed materials, and performing first purification, second decomposition and third purification. The volume ratio of the crushed materials to deionized water is 1:3. Nano-scale collagen acquired through three purification steps and a particle collisions instrument is detected by SGS andPUNY, so that the nano-scale collagen is free from oestrone, chloramphenicol, progesterone, salmonella, methyltestosterone, macrodantin metabolite, fat, estradiol and estriol. According to the method,slurry acquired after treatment of the fish skins is used for extraction of the collagen, the purification rate and the purity of the collagen can be obviously increased, the slurry is purified by anano-scale collagen purification device to obtain nano-scale mixed powder, collision of the nano-scale mixed powder is implemented by the particle collisions instrument to obtain the nano-scale collagen with uniform molecular weight, and the absorption rate of the nano-scale collagen can reach 96%.