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Process for preparing ginsenosides Rh2 and Rh3 from stems and leaves of pseudoginseng root

A technology of ginsenosides and process methods, which is applied in the direction of steroids, digestive system, blood diseases, etc., and can solve problems such as complex processes, affecting product quality, and high cost

Inactive Publication Date: 2010-09-15
YUNNAN HEIYAO PLANT TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disclosed method of extracting ginsenosides Rh2 and Rg3 using the stems and leaves of Panax notoginseng as raw materials has many problems. Various chemical reagents are used in each step of the method, such as sodium carbonate neutralizing acid, n-butanol extraction, methanol-chloroform Dissolving, eluting with chloroform-methanol-ethyl acetate-water (2:2:4:1, lower layer), etc., these non / organic solvents will be partially brought into the product, affecting product quality
The process is more complicated and the cost is higher, which also exists objectively

Method used

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  • Process for preparing ginsenosides Rh2 and Rh3 from stems and leaves of pseudoginseng root
  • Process for preparing ginsenosides Rh2 and Rh3 from stems and leaves of pseudoginseng root
  • Process for preparing ginsenosides Rh2 and Rh3 from stems and leaves of pseudoginseng root

Examples

Experimental program
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Effect test

Embodiment 1

[0043] (1) Extract the total saponins of Panax notoginseng

[0044] Stems and leaves of Panax notoginseng→boiled extraction→adsorption→elution→concentration→dilute extract of Panax notoginseng total saponins,

[0045] Decoct the stems and leaves of Panax notoginseng with water for three times, each time for 2 hours, the temperature is 90°C, filter after the extraction is completed, combine the three times of filtrate to absorb on the ADS3 macroporous adsorption resin column, wash with water, elute with 70% ethanol solution, elute The liquid is concentrated under reduced pressure until it has no alcohol smell, and the dilute extract of Panax notoginseng saponins is obtained.

[0046] (2) Preparation of diol group saponins

[0047] Panax notoginseng saponins dilute extract→adsorption→elution→concentration→drying→crushing→diol group saponins,

[0048] The dilute extract of Panax notoginseng total saponins is diluted 3 times with water, adsorbed on an AB8 resin column, washed wi...

Embodiment 2

[0053] (1) Extract the total saponins of Panax notoginseng

[0054] Stems and leaves of Panax notoginseng→boiled extraction→adsorption→elution→concentration→dilute extract of Panax notoginseng total saponins,

[0055] Add the stems and leaves of Panax notoginseng to decoct and extract three times, each time for 5 hours, the temperature is 75°C, filter after the extraction is completed, combine the three times of filtrate to absorb on the ADS3 macroporous adsorption resin column, wash with water, elute with 20% ethanol solution, elute The liquid is concentrated under reduced pressure until it has no alcohol smell, and the dilute extract of Panax notoginseng saponins is obtained.

[0056] (2) Preparation of diol group saponins

[0057] Panax notoginseng saponins dilute extract→adsorption→elution→concentration→drying→crushing→diol group saponins,

[0058] The dilute extract of Panax notoginseng total saponins is diluted 5 times with water, adsorbed on AB8 resin column, washed w...

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Abstract

The invention relates to a process for preparing ginsenosides Rh2 and Rh3 by using stems and leaves of pseudoginseng root as raw materials, which comprises the following technical flows: (1), extracting saponin from the leaves of the pseudoginseng root, namely cooking the stems and leaves of the pseudoginseng root with water for extraction, absorbing, eluting and concentrating to obtain a thin extract of the ginsenosides of the pseudoginseng root; (2), preparing diol saponins, namely absorbing, eluting, concentrating, drying and crushing the thin extract of the ginsenosides of the pseudoginseng root to obtain the diol saponins; and (3), acid hydrolysis, namely, performing acid hydrolysis on the diol saponins, absorbing, washing with water, eluting, concentrating and drying to obtain a mixture which contains the ginsenosides Rh2 and Rh3. The process of the invention is simple and feasible, high in purification rate and good in quality, and reaches industry standards.

Description

technical field [0001] The invention relates to a process for extracting effective ingredients for plant medicine, in particular to a method for preparing ginsenosides Rh2 and Rg3 by using the stems and leaves of Panax notoginseng as raw materials. Background technique: [0002] Ginseng is a world-renowned traditional Chinese medicine. As early as 4,000 years ago, our ancestors used ginseng to prevent and treat diseases. In the early 1990s, Japanese scholar Kitagawa Isun discovered that an active substance in ginseng--ginsenoside Rg3 has a selective Inhibition of tumor cell invasion and metastasis. There are more than 40 kinds of saponins in ginseng, but Rg3 does not exist in fresh or naturally dried ginseng medicinal materials, and the content in red ginseng is only 3 / 100,000. Ginsenoside Rg3 not only has anti-cancer and anti-cancer effects, but also has pharmacological effects such as improving immunity, protecting the liver, anti-fatigue, protecting brain nerve cells, an...

Claims

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Application Information

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IPC IPC(8): C07J9/00A61P35/00A61P37/02A61P1/16A61P25/00A61P7/02
Inventor 张凯张吉华
Owner YUNNAN HEIYAO PLANT TECH DEV
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