Nano-scale collagen purification method capable of reaching absorption rate of 96%

Abstract

The invention discloses a nano-scale collagen purification method capable of reaching the absorption rate of 96%. The method includes the steps: crushing fish skins, and removing impurities; placing crushed materials into a low-temperature stirrer, stirring and mixing mixture for 30 minutes according to the volume ratio of the crushed materials to deionized water, standing for 10 minutes, removingupper floats, and placing mixture into a filter device to perform adsorption filtration; collecting filtered crushed materials, and performing first purification, second decomposition and third purification. The volume ratio of the crushed materials to deionized water is 1:3. Nano-scale collagen acquired through three purification steps and a particle collisions instrument is detected by SGS andPUNY, so that the nano-scale collagen is free from oestrone, chloramphenicol, progesterone, salmonella, methyltestosterone, macrodantin metabolite, fat, estradiol and estriol. According to the method,slurry acquired after treatment of the fish skins is used for extraction of the collagen, the purification rate and the purity of the collagen can be obviously increased, the slurry is purified by anano-scale collagen purification device to obtain nano-scale mixed powder, collision of the nano-scale mixed powder is implemented by the particle collisions instrument to obtain the nano-scale collagen with uniform molecular weight, and the absorption rate of the nano-scale collagen can reach 96%.

Description

technical field [0001] The invention relates to the technical field of collagen purification methods, in particular to a nanoscale collagen purification method with an absorption rate of 96%. Background technique [0002] Collagen is a biological polymer substance, the English scientific name Collagen. Plays the role of binding tissue in animal cells. It is one of the most critical raw materials in the biotechnology industry, and it is also the best biomedical material with a huge demand. Its application areas include biomedical materials, cosmetics, food industry, research purposes, etc. Collagen is an extracellular protein. It is a fibrous protein twisted into a helical shape by 3 peptide chains. Collagen is the most abundant protein in the human body. Dr. J. Brandt, the father of collagen in the world, It is asserted: "The process of human aging is the process of collagen loss!" Collagen accounts for more than 30% of the total protein in the body. Collagen is rich in ...

AI Technical Summary

Problems solved by technology

Collagen generally exists in the skin of livestock and livestock, but high-grade collagen is fish collagen extracted from fish skin. Currently, the conventional methods for preparing collagen are mainly subject to two major processes: enzymatic hydrolysis and hydrolysis. Due to insufficient purification precision and low purification rate, there are many impurities in the collagen extracted by these two methods, which seriously affects the quality of collagen, and its molecular weight can only reach the small molecule level.

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