Snail enzyme preparation method

A helicase and snail technology, applied in biochemical equipment and methods, enzymes, lyases, etc., can solve the problems of poor operability, low purification rate, complicated process, etc., and achieve the effect of low price, high purification rate and simple process

Inactive Publication Date: 2015-06-24
威海圣奥罗生物科技有限公司
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  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention aims to solve the technical problems of the existing helicase extraction method with complex process, poor operability, low purification rate and high cost, and provides a method for preparing helicase with simple process, strong operability, high purification rate and low cost

Method used

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  • Snail enzyme preparation method

Examples

Experimental program
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Embodiment Construction

[0019] Such as figure 1 Shown:

[0020] (1) Snail selection:

[0021] Choose more than 14 grams of fresh brown cloud agate snails (scientific name: Achatina Fulica).

[0022] (2) Snail cleaning:

[0023] Put the snails in stocking boxes filled with small holes at the bottom. Each stocking box utilizes about 30% of the space, starve them for 3 days, and wash them with clean water at a temperature of 18°C ​​to 28°C every morning and evening to wash off feces and sundries. , Cleaning can be done with a rag, slightly turning the snails, and carefully picking out the dead snails every time you clean. For the clean water used for flushing, the temperature is preferably 23°C.

[0024] (3) Shelling of snails:

[0025] Take 50KG cleaned fresh snails, crack the snail shells on the workbench, and carefully peel off the shells.

[0026] (4) Cancellation of the glands:

[0027] Cut the body wall along the digestive tract of the snail to peel off the digestive glands.

[0028] (5) p...

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Abstract

The invention relates to a snail enzyme preparation method and solves problems that an existing snail enzyme extraction method is complex in process, poor in operability, low in purification rate and high in cost. The snail enzyme preparation method includes the steps of selection of fresh achatina fulica, cleaning, shelling, digestive gland removal, digestive juice pumping, primary filtering, centrifuging, secondary filtering, vacuum freeze dehydration, glue milling and storage. The method is widely applicable to preparation of snail enzyme.

Description

technical field [0001] The invention relates to a method for preparing an enzyme, in particular to a method for preparing a helicase. Background technique [0002] Helicase is a mixed enzyme prepared from the snail's pouch and digestive tract, which contains more than 20 kinds of enzymes such as cellulase, pectinase, amylase, and protease. Helicase is a valuable enzyme, it can be used to break down the yeast cell wall, so it is widely used in the research of cell biology and genetic engineering; it can be used as a feed additive to improve the digestibility of feed; it can also be used For juice clarification, orange peeling, jam making, etc. Snail enzymes contain 9 common enzymes: cellulase, hemicellulase, pectinase, alpha amylase, mannase, sucrase, galactanase, proteolytic enzyme, Amino acid transferase and other mixed enzymes with biological activity. However, the current extraction method of helicase has the defects of complex process, poor operability and low purific...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/88C12N9/64C12N9/42C12N9/26C12N9/18
CPCC12N9/88C12N9/18C12N9/6405
Inventor 刘斌杨磊
Owner 威海圣奥罗生物科技有限公司
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