Method for preparing high-content tuna anserine by adopting membrane separation and application of tuna anserine

A tuna, high-content technology, applied in the direction of semi-permeable membrane separation, application, material separation, etc., can solve the problems of high cost, unfavorable industrial production, obtaining high-purity tuna anserine, and many raw materials, etc., and achieve important social and economic benefits. Effect

Active Publication Date: 2021-04-02
SHANDONG RUIZHI BIOTECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, there are few studies on tuna anserine both at home and abroad, such as the CN101417119 invention patent application disclosed on April 29, 2009 "medicine composition and packaging for rapidly reducing uric acid in blood and anserine for rapidly reducing blood The use of uric acid in the medium", CN108576367A invention patent application published on September 28, 2018 "Fish polypeptide dry powder containing anserine, uric acid peptide composition, beverage and preparation method", CN108727269A invention published on November 2, 2018 The patent application discloses "a preparation method of N(τ)-methyl-L-histidine derivatives and its application in the synthesis of anserine", and another example is the CN109748874A invention patent application published on May 14, 2019 "A preparation method for anserine and its intermediates" and CN110346471A invention patent application published on October 18, 2019 "A high-performance liquid chromatography method for determining the content of carnosine and anserine in fish heads" and April 2020 The CN111004181A invention patent application published on March 14 discloses "a preparation method for tuna anserine", but the inventors found that no matter whether anserine is isolated from natural raw materials or synthesized by chemical or biological methods, there is a problem due to raw material pretreatment technology. And the problem that the cost that separation technology causes is very high, the synthetic anserine also has purity problem, and above-mentioned preparation method uses more raw materials, and preparation process is complicated, is unfavorable for large-scale industrialized production and obtaining high-purity tuna anserine

Method used

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  • Method for preparing high-content tuna anserine by adopting membrane separation and application of tuna anserine
  • Method for preparing high-content tuna anserine by adopting membrane separation and application of tuna anserine
  • Method for preparing high-content tuna anserine by adopting membrane separation and application of tuna anserine

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Embodiment 1

[0053] A kind of method adopting membrane separation to obtain high-content tuna anserine, concrete steps are as follows:

[0054] S1. Select 2.0kg of freshly frozen tuna meat.

[0055] S2. The raw materials are thawed by using ice water thawing method.

[0056] S3. The tuna meat is quickly crushed and beaten.

[0057] S4. Put the beaten tuna slurry in the boiling water prepared in advance (the first time the tuna common meat slurry is mixed with pure water at a mass ratio of 1:10), the temperature is controlled at 100°C, and the extraction time is 2h. 200 mesh sieves are sieved and filtered for the first time to obtain filtrate a, and the fish paste filtered by the first sieve continues to be dropped into boiling water for the second extraction (the second time tuna common meat fish paste and pure water are in a mass ratio of 1 : 10 mixing), the temperature is controlled at 100°C, the extraction time is 2h, and a 200-mesh sieve is used to sieve and filter for the second tim...

Embodiment 2

[0065] A kind of method adopting membrane separation to obtain high-content tuna anserine, concrete steps are as follows:

[0066] S1. Select 2.0kg of freshly frozen tuna meat.

[0067] S2. The raw materials are thawed by using ice water thawing method.

[0068] S3. The tuna meat is quickly crushed and beaten.

[0069] S4. Put the beaten tuna slurry in the boiling water prepared in advance (the first time the tuna common meat slurry is mixed with pure water at a mass ratio of 1:10), the temperature is controlled at 100°C, and the extraction time is 2h. 200 mesh sieves are sieved and filtered for the first time to obtain filtrate a, and the fish paste filtered by the first sieve continues to be dropped into boiling water for the second extraction (the second time tuna common meat fish paste and pure water are in a mass ratio of 1 : 10 mixing), the temperature is controlled at 100°C, the extraction time is 2h, and a 200-mesh sieve is used to sieve and filter for the second tim...

Embodiment 3

[0077] A kind of method adopting membrane separation to obtain high-content tuna anserine, concrete steps are as follows:

[0078] S1. Select 2.0kg of freshly frozen tuna meat.

[0079] S2. The raw materials are thawed by using ice water thawing method.

[0080] S3. The tuna meat is quickly crushed and beaten.

[0081] S4. Put the beaten tuna slurry in the boiling water prepared in advance (the first time the tuna common meat slurry is mixed with pure water at a mass ratio of 1:10), the temperature is controlled at 100°C, and the extraction time is 2h. The 200-mesh sieve is sieved and filtered for the first time to obtain filtrate a, and the remaining fish paste is continuously put into boiling water for the second extraction (the second time tuna common meat fish paste is mixed with pure water at a mass ratio of 1:10), the temperature The temperature was controlled at 100°C, the extraction time was 2 hours, and a second sieve was performed with a 200-mesh sieve to obtain th...

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Abstract

The invention provides a method for preparing high-content tuna anserine by adopting membrane separation and application of the tuna anserine, and belongs to the technical field of protein peptide extraction and purification. The method comprises the steps: smashing and pulping tuna meat, then placing tuna pulp in boiling water, performing screening and filtering twice, combining filtrates obtained twice, performing ultrafiltration membrane filtering three times, taking trapped fluid obtained in the third time, and performing concentration, smashing, heating, and reflux extraction to obtain the tuna anserine. According to the method, rich tuna fishery resources in China and the medical health care functions of the anserine contained in tuna meat are fully utilized, the industrial production and processing technology level and additional value of tuna anserine products are effectively improved, the production and preparation process is simplified, and the production efficiency is improved. Meanwhile, the pollution problem and the production cost for producing the product are reduced, and the varieties of tuna anserine products on the market are enriched, so that the high-content tuna anserine has a good practical application value.

Description

technical field [0001] The invention belongs to the technical field of protein peptide extraction and purification, and in particular relates to a method for preparing high-content tuna anserine by membrane separation and its application. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Tuna is an important economic fish in the world and one of the most important marine fish species in my country. It is widely distributed in the world's warm water fisheries and occupies an important economic position in the development of fishery economy. Tuna is also called tuna, and its English name is Tunas. It belongs to the bony fish class, the perciformes, and the scombroid family. I...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D233/64B01D61/14G01N30/02A23L33/18
CPCC07D233/64G01N30/02A23L33/18A23V2002/00G01N2030/027B01D61/146A23V2200/30A23V2250/55
Inventor 张永刚徐振鲁孙绪春李芳于美霞
Owner SHANDONG RUIZHI BIOTECH
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