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Method for extracting refined cordycepin and cordycepin polysaccharide from cordyceps mititaris

A technology for Cordyceps polysaccharide and Cordyceps militaris, applied in chemical instruments and methods, sugar derivatives, food preparation and other directions, can solve problems such as difficulty in large-scale practice, long extraction time, inconvenience in industrial production, etc., to reduce production costs and ensure biological Activity, the effect of increasing utilization

Active Publication Date: 2008-02-20
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with traditional extraction methods, supercritical CO 2 Extraction overcomes the shortcomings of the former, such as long extraction time, high temperature, and open system, and can extract more polar components, which can more truly and comprehensively reflect the chemical components of medicinal materials. However, due to supercritical CO 2 The cost of extraction technology used in the extraction stage of cordycepin is too high and it is difficult to carry out large-scale practice
Chinese patent CN1560085A discloses a process for extracting Cordyceps polysaccharides from culture medium leftovers produced in the artificial cultivation of Cordyceps. This method can turn waste into treasure, reduce waste of resources, and improve economic benefits. However, chloroform is used in the polysaccharide preparation process. and other organic solvents, which is not conducive to food safety
At present, there is no report on the patent on the comprehensive extraction and refining of the active components of Cordyceps militaris. Most of the existing extraction techniques are single or intermittent, often simply extracting cordycepin or cordyceps polysaccharides through a set of systems, so the discontinuous extraction technique does not It is convenient for industrialized production; in addition, due to too many extraction and separation steps, the total content of active ingredients extracted by the prior art is not high, resulting in a great waste of resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Crush 1kg of Cordyceps militaris fruiting bodies through a 60-mesh sieve, add 5000 ml of 95% ethanol to the crushed raw material and extract 3 times by cold infiltration, 3 hours each time, and initially separate impurities such as alcohol-soluble pigments in the form of filtrate Come out, discard the filtrate, and the filtrate can be reserved for other purposes. The filter residue was recovered and dried in vacuum at 55° C., and the dried filter residue was mixed with 20% ethanol at a weight ratio of 1:10, and ultrasonic-assisted extraction was performed 3 times, each time for 40 minutes. After the extraction, filter, combine multiple filtrates, concentrate under reduced pressure, add 95% ethanol to make the ethanol content in the solution 80% (by weight), and let stand for 8 hours.

[0021] Take the supernatant, concentrate it to dryness under reduced pressure, add water to dissolve it, adjust the pH value to 3.5, put it on a 732-type cation exchange column and use 0....

Embodiment 2

[0024] 0.5kg of Cordyceps militaris fruiting bodies were crushed through a 40-mesh sieve, and 3000 milliliters of absolute ethanol was added to the pulverized raw materials to extract twice by cold infiltration, each time for 4 hours, and impurities such as alcohol-soluble pigments were initially extracted in the form of filtrate. Separated, discard the filtrate, the filtrate can be reserved for other purposes. The filter residue was recovered and dried in vacuum at 55° C., and the dried filter residue was mixed with 30% ethanol at a weight ratio of 1:20, and ultrasonic-assisted extraction was performed twice, each time for 60 minutes. After the extraction is finished, filter, combine multiple filtrates, concentrate under reduced pressure, add absolute ethanol to make the ethanol content in the solution 80% (by weight), and let it stand for 12 hours.

[0025] Take the supernatant, concentrate it to dryness under reduced pressure, add water to dissolve it, adjust the pH value t...

Embodiment 3

[0028] 0.8kg Cordyceps militaris fruiting bodies were crushed through a 40-mesh sieve, and 4500 milliliters of absolute ethanol was added to the pulverized raw materials to extract twice by cold infiltration, each time for 5 hours, and impurities such as alcohol-soluble pigments were initially extracted in the form of filtrate. Separated, discard the filtrate, the filtrate can be reserved for other purposes. The filter residue was recovered and dried in vacuum at 55° C., and the dried filter residue was mixed with 20% ethanol at a weight ratio of 1:25, and ultrasonic-assisted extraction was performed twice, each time for 90 minutes. After the extraction is finished, filter, combine multiple filtrates, concentrate under reduced pressure, add absolute ethanol to make the ethanol content in the solution 70% (weight), and let stand for 16 hours.

[0029] Take the supernatant, concentrate it to dryness under reduced pressure, add water to dissolve it, adjust the pH value to 3.0, an...

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PUM

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Abstract

The present invention relates to a method for extracting the purificatory cordycepin and the cordyceps amylase from the cordyceps militaris, belonging to the fields of medicines and health protection and chemical engineering. The cordyceps militaris powder is extracted and filtrated by a cooling seep method, the filter residue is mixed with ethanol, the supersonic wave assists to extract and offcenter, the supernatant has the processes of concentration and alcohol-precipitating, then the alcohol-precipitated supernatant has the processes of decompression, concentration and elution, the eluted liquid has the processes of concentration and crystallization and recrystallization using the n-butyl alcohol, at last the purificatory amylase is obtained after freezing and drying. The preparation progress of the present invention only uses water and ethanol as the solvent, thus reducing the pollution, the resin is capable of being reused a plurality of times with low cost. The present invention fully exploits the biological activity ingredients of the cordyceps militaris, realizes the coextraction of the cordycepin and the cordyceps amylase, guarantees the biological activities of the cordycepin and the cordyceps amylase, farthest increases the utilization rate of the raw material to the largest extent, reduces the manufacturing cost. The present invention is suitable for the large-scale industrialized manufacture.

Description

1. Technical field: [0001] The invention relates to a method for extracting refined cordycepin and cordyceps polysaccharide from Cordyceps militaris, belonging to the fields of medicine, health care and chemical industry. It generally relates to the extraction and separation of substances, and more specifically, the present invention relates to a method for extracting refined cordycepin and cordyceps polysaccharide from Cordyceps militaris. 2. Background technology: [0002] Cordyceps militaris is a precious Chinese herbal medicine, with a flat taste and sweet taste, which has the effects of nourishing the lungs and kidneys, stopping bleeding and resolving phlegm. Research results in recent years have shown that Cordyceps militaris is one of the important artificial substitutes of Cordyceps sinensis. It can reduce blood fat, prevent arteriosclerosis, protect heart and brain tissue, calm and hypnotize, enhance macrophage activity, anti-cancer, anti-inflammatory and antibacter...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/28A23L1/09A23L1/30C07H1/08C07H19/16C08B37/00
Inventor 刘春泉宋江峰李大婧
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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