53 results about "Thunnus sp." patented technology

The invention discloses a tuna pond culture strategy optimization method and system based on meteorological influence analysis, and the method comprises the steps: collecting regional meteorological data and pond culture monitoring data, and constructing a multi-modal pond ecological feature vector; establishing a weather-water body dynamic coupling model by using a space-time diagram neural network, and generating water body situation response prediction information; a breeding regulation and control strategy is made based on the prediction information, and strategy self-adaptive optimization is achieved through real-time monitoring data; evaluating the anti-disaster capability of the tuna after the meteorological influence is finished, screening high-quality germplasm resources by combining genetic analysis, and generating an anti-disaster breeding scheme. According to the method, full-chain optimization from meteorological early warning to culture regulation and control to genetic breeding is achieved, and the climate toughness and economic benefits of tuna pond culture are comprehensively improved.

Provided herein are altered cell lines comprising a suspension cell line adapted from an adherent cell line having a different expression profile from a corresponding non-altered adherent cell line, methods for generating altered cell lines, and methods of characterizing altered expression profiles for a gene, a transcript, or a protein in an altered cell line.

The invention relates to the technical field of aquatic product processing, in particular to an integrated low-loss processing method of tuna fillets. The method comprises the following steps: performing low-temperature radio-frequency quick thawing on cryopreserved tuna fish fillets, taking out the tuna fish fillets until the central temperature of the tuna fish fillets reaches 4 DEG C, and quickly cutting the tuna fish fillets into blocks; soaking the fish blocks in a composite fresh-keeping soaking solution at 3-5 DEG C, and performing low-temperature ultrasonic treatment; and washing the soaked fish blocks, draining the surfaces of the fish blocks, loading the fish blocks into a vacuum packaging bag according to specification requirements, vacuumizing, putting the vacuum packaging bag into a water bath kettle, carrying out temperature gradient type low-temperature curing, and taking out to obtain a cured finished product of the tuna fish fillet. Through combined application of a low-temperature radio-frequency quick thawing technology and a vacuum low-temperature low-loss curing technology, the obtained tuna fillet cured product not only ensures the quality of fish meat, but also effectively inhibits histamine increase and fat oxidation of the cured fish meat during low-temperature storage; the method is of great significance in promoting quality improvement and diversified development of tuna products.

The invention relates to the technical field of tuna product processing, and discloses a composite seasoning processing method for reducing the fishy smell of tuna products, which comprises the following steps: trimming, cutting and rinsing tuna raw materials to obtain tuna meat blocks, preparing a composite pickling solution, mixing the tuna meat blocks with the composite pickling solution at low temperature, standing, and then cooling to obtain the tuna products. The preparation method comprises the following steps: pickling tuna meat blocks to obtain pickled tuna meat blocks, carrying out high-temperature shaping treatment on the pickled tuna meat blocks, then carrying out forced cooling treatment to obtain cooled tuna meat blocks, and carrying out low-temperature drying treatment on the cooled tuna meat blocks to obtain the tuna product. The method solves the problems that when high-temperature baking is adopted in an existing tuna processing technology, the outer part is scorched and the inner part is generated due to uneven heating power, and the product quality is unstable and the taste is poor due to the fact that fishy smell substance residues cannot be fundamentally eliminated by depending on a traditional pickling method.

The invention provides traditional Chinese medicine powder for improving the transfer survival rate of yellow fin tuna, which is prepared by weighing the following components in percentage by mass: 15-20% of radix bupleuri, 10-15% of radix puerariae, 15-20% of liquorice, 5-10% of Chinese herbaceous peony, 5-10% of blackberry lily, 5-10% of fructus forsythiae, 5-10% of poria cocos, 5-10% of rhizoma anemarrhenae, 5-10% of pericarpium citri reticulatae and 20-25% of gypsum, totaling 100%. All the materials pass through a 200-mesh screen and are uniformly mixed to obtain the traditional Chinese medicine powder for improving the transfer survival rate of the yellow fin tuna. The traditional Chinese medicine powder takes common Chinese herbal medicines as main raw materials, has the characteristics of rich resources, wide raw material sources, easiness in obtaining and the like, is free of toxic and side effects, free of hormones, difficult to pollute the environment, free of residues in the bodies of the yellow fin tunas, safe to use, simple to operate, low in cost and suitable for popularization, and has a wide application prospect. The survival rate of the yellow fin tuna in the transportation process can be increased, the immunity of the fish body can be adjusted, and the anti-stress capability of the fish body is enhanced.

The invention provides a method for extracting goose carnosine and / or carnosine. The method comprises the steps of low-salt water extraction and high-salt water extraction under alkaline and heating conditions. The extraction method of the goose carnosine and / or carnosine provided by the invention is simple to operate, low in cost and easy for large-scale industrial use.

The present application relates to the technical field of biology, and particularly relates to an antioxidant peptide and ACE inhibitory peptide from tuna, and a preparation method and application thereof. The amino acid sequence of the polypeptide provided by the present application is shown as SEQ ID NO. 1-6. The present application takes tuna skin waste as raw material, obtains tuna skin collagen through enzymolysis, then performs liquid chromatography-tandem mass spectrometry identification and molecular docking screening on the tuna skin collagen enzymolysis product, and obtains polypeptides with antioxidant activity and polypeptides effectively inhibiting ACE activity. The method simplifies the protein extraction step. Meanwhile, the method can improve the deep processing level of the tuna industry, achieve high-value utilization, and reduce the environmental pressure on processing waste.

This invention provides a method for preparing tuna skin collagen peptide zinc ion chelate, belonging to the field of marine biological functional product processing. The method mainly involves hydrolyzing tuna skin using a complex protease to obtain collagen active peptides, and then chelating these active peptides with a zinc ion solution to finally obtain the tuna skin collagen peptide zinc ion chelate. The preparation method provided by this invention is simple and convenient to operate, low in cost, and has good safety. The yield of the chelate prepared by this invention is as high as 70% or more, the chelation rate is over 85%, and the molecular weight distribution of the chelate has a certain regularity, with the mass percentage of molecules with a molecular weight distribution of 3-5 kDa exceeding 49%. The chelate prepared by this invention, with active peptides chelated with zinc ions, can exert a synergistic effect, not only enhancing the biological activity of the original polypeptide components but also greatly reducing the toxic side effects caused by direct zinc ion supplementation.

The invention discloses a tuna red meat immunity enhancing peptide and application thereof in enhancing immunity, and belongs to the technical field of biology. According to the invention, polypeptides with immunocompetence are extracted from tuna red meat, and the polypeptides comprise FLQGF and LAKFPDF. Experiments prove that the polypeptide prepared by the invention has no obvious toxic effect on cells, and can improve the proliferation rate and the phagocytic rate of macrophages and the release rate of NO to different degrees. An in-vitro immunomodulatory activity result shows that the LAKFPDF and the FLQGF can activate a TLR4 dependent NF-kB / MAPK signal channel, regulate and control the immune response of macrophages and play an immunomodulatory role. Research results of the invention provide a basis for high-value development and utilization of tuna red meat, and provide a new resource for preparation of immunopotentiation products.

This is a large-fish insulated bag for transporting and storing large fish, such as tuna, while keeping them cool. It measures 2550 mm wide and 1550 mm long in the front view. As shown in the reference oblique view, this bag is formed as a single sheet and is used by folding it in half along the fold line indicated by the dashed line in the figure. This bag is made of two overlapping, flexible, and waterproof layers of fabric, sandwiched between them and a highly insulating material such as urethane. When in use, the left, right, and top edges are sealed with a series of waterproof zippers, preventing water leakage from the interior. Four water taps are provided on the front for releasing water from the interior. As shown in the reference oblique view, the unfolded bag can be placed halfway (upper right or lower left of the fold line) and folded back as described above. The bag can then be placed inside the bag by closing the waterproof zipper.

The invention provides iron binding peptides based on tuna blood combined meat. The amino acid sequences of some polypeptides are SEQ ID NO: 1-6. The polypeptide provided by the invention can be used for preparing a product for treating iron-deficiency anemia. According to the tuna red meat hematopoietin provided by the invention, an organic iron compound with high bioavailability is formed through the chelation of specific polypeptide iron ions in preparation of a blood replenishing product, so that the absorption efficiency of intestinal tracts on iron is remarkably improved. A carrier formed by the polypeptide can protect iron ions from being damaged by gastric acid, and the iron ions are delivered to an absorption part in a targeted mode, so that the hemoglobin synthesis efficiency is improved by 40% or above. The natural polypeptide ligand disclosed by the invention avoids gastrointestinal irritation of a traditional iron supplementing agent, and is not interfered by absorption of dietary factors such as phytic acid and tannin. The tuna processing by-product is used as the raw material to prepare the functional blood-enriching peptide, so that high-value utilization of fishery resources is realized, and an eco-friendly new iron-enriching source is provided.

Provided herein are altered cell lines comprising a suspension cell line adapted from an adherent cell line having a different expression profile from a corresponding non-altered adherent cell line, methods for generating altered cell lines, and methods of characterizing altered expression profiles for a gene, a transcript, or a protein in an altered cell line.

An in-vitro tissue model for use with a test article includes a tissue sample that includes homogeneous tuna meat having an isotropic thermal response, wherein the tuna meat is characterized by a plurality of visually distinct color states, each color state corresponding to a respective temperature to which the tuna meat has been heated within a range of 43° C. to 60° C., thereby providing a visual indication of the thermal effect of the test article within said range.

This invention belongs to the field of functional marine food processing technology, specifically involving a method for producing a composite sausage made from Antarctic krill, tuna, and collagen, comprising the following steps: (1) preparation of Antarctic krill meat, (2) preparation of tuna blood-infused meat, (3) preparation of low-temperature raw pork skin collagen paste, (4) preparation of krill-derived composite enzyme solution, (5) mixing of meat materials and low-temperature enzymatic hydrolysis, (6) low-temperature chopping, (7) sausage filling and quick-freezing, and (8) thawing and maturation of the product. This invention uses Antarctic krill meat, tuna blood-infused meat, and low-temperature raw pork skin collagen paste as core raw materials, and utilizes krill endogenous composite enzymes for low-temperature enzymatic hydrolysis to produce a composite sausage with uric acid-lowering function, high protein content, and high unsaturated fatty acid content, providing a safe, convenient, and daily-consumable functional marine food for people with hyperuricemia.

The invention relates to the technical field of fishery. A small tuna on-ship temporary rearing device comprises a temporary rearing fishpond, the temporary rearing fishpond comprises a water inlet pond on the front side, a fish storage box in the middle and a fish releasing pond on the rear side, and a guiding device is arranged in the temporary rearing fishpond; the fish storage box is of a multi-layer structure, all layers of the fish storage box are separated through first partition plates, water permeable holes are formed in the surfaces of the first partition plates, and a plurality of limiting devices used for limiting tuna movement are arranged on each layer of the fish storage box at intervals. The invention provides a device for temporarily rearing small tunas on a ship, which can effectively pretreat the conditions of spalling of mucus layers and body surface scratching of small tunas in the process of temporarily rearing the small tunas on the ship and increase the survival rate and the survival state of the small tunas in the process of temporarily rearing the small tunas on the ship. The technical problems that in the prior art, an existing small tuna on-ship temporary rearing device is single, collision is likely to happen in the on-ship temporary rearing process, a surface mucus layer is likely to fall off, the body surface is likely to be scratched, and the survival rate and the survival state are not ideal enough are solved.

The invention discloses a fish peptide compound with anti-oxidation, anti-wrinkle and whitening functions as well as a preparation method and application thereof, belongs to the technical field of functional peptide application, and provides the fish peptide compound with the anti-oxidation, anti-wrinkle and whitening functions. Comprising 40 to 50 parts of fish collagen peptide, 36 to 40 parts of elastin peptide, 18 to 21 parts of collagen tripeptide, 14 to 17 parts of tuna active peptide, 5 to 6 parts of avocado extract, 2 to 4 parts of fish peptide and 2.6 to 3.0 parts of rye seed extract. According to the fish peptide compound with the antioxidant, anti-wrinkle and whitening functions as well as the preparation method and the application, the fish-derived functional peptide and the plant extract are reasonably compounded, all the components play a synergistic role, and the prepared fish peptide compound has the antioxidant, anti-wrinkle and whitening effects at the same time.

The present application belongs to the technical field of bioengineering, and particularly relates to a tuna fish bone antioxidant oligopeptide and a preparation method thereof.The tuna fish bone antioxidant oligopeptide has good scavenging activity, and has good thermal stability and digestion resistance; the mechanism of action is to promote the release of nitric oxide (NO), improve the levels of superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), catalase (CAT) and total antioxidant capacity (T-AOC), and reduce the levels of malondialdehyde (MDA) and lactate dehydrogenase (LDH); JQYG-1 can protect HUVEC cells from oxidative damage induced by hydrogen peroxide; JQYG-1 can exert an antioxidant effect and protect vascular endothelial cells, and can provide a candidate drug for the prevention and treatment of cardiovascular and cerebrovascular diseases such as hypertension, coronary heart disease, cerebral thrombosis, myocardial infarction, atherosclerosis and heart failure.

The invention provides a method for enriching tuna cooking liquor goose carnosine, and relates to the field of high-value utilization of aquatic product processing byproducts. The enrichment method of the tuna cooking liquor goose carnosine comprises the following steps: taking the tuna cooking liquor, and carrying out pretreatment to remove suspended solids so as to obtain a pretreatment solution; filtering the pretreatment liquid through a ceramic membrane with the molecular weight cut-off of 3000-5000Da, and collecting permeate liquid; adding the permeate into a phosphate buffer solution for dynamic dilution, then filtering through an organic ultrafiltration membrane with the molecular weight cutoff of 150-200Da, and collecting the trapped fluid, so as to obtain enriched goose carnosine; wherein the dynamic dilution is to repeatedly add a phosphate buffer solution in the membrane filtration process, so as to avoid coprecipitation of purine and goose carnosine under high concentration. According to the method, coprecipitation of purine and the goose carnosine under high concentration is effectively avoided through the synergistic effect of multi-stage membrane separation (the combination of the ceramic membrane and the organic ultrafiltration membrane) and the dynamic dilution process, the yield of the goose carnosine can reach 1.28 g / L or above, and the purity of the goose carnosine can reach 86% or above.

1. Name of the designed product: packing box (frozen tuna block). 2. Use of the designed product: for containing fish. 3. Design points of the designed product: combination of shape and pattern. 4. Picture or photo best showing the design points: perspective view.

The present application relates to aquatic transportation technology, and aims to provide a tuna larva live-keeping transfer device. The device comprises a core live-keeping system, a stress source control system and an intelligent centralized monitoring system arranged in a refrigerated container; the core live-keeping system comprises a fish tank main body, a water flow dynamics module and a water quality maintenance module, the stress source control system comprises an illumination module, a vibration and noise reduction module and an anti-collision module, and the intelligent centralized monitoring system comprises a camera monitoring module, a sensor array and a feedback control module; the fish tank main body is a closed oval ring-shaped pool body, a plurality of water inlets and outlets are uniformly arranged on the side wall, and are connected to a circulating water pump through pipelines respectively. The present application can meet the high metabolic demand of tuna larvae sustained swimming in a limited transfer water space, block the stress inducement of the whole transfer process from the source, realize the full closed loop intelligent control of the transfer process, reduce the stress reaction incidence and mechanical damage rate of tuna larvae, and improve the survival rate of long distance transfer.

The invention relates to the technical field of net cage fishing devices, and discloses a large-scale net cage fishing device for yellow fin tuna culture and a fishing method.The large-scale net cage fishing device comprises a piston rod fixedly connected to the inner wall of a piston sleeve, a plurality of water outlet holes are formed in the outer wall of the piston sleeve, and a plurality of through grooves are formed in the inner wall of the piston sleeve; a plurality of water outlet holes II are formed in the outer wall of the piston cylinder; when the bottom ring moves to the connecting rings, the connecting rings can be driven to move together, at the moment, along with movement of the connecting rings, the two adjacent connecting rings at the bottommost end can get close to each other, and at the moment, the piston sleeve and the piston barrel which are arranged between the two adjacent connecting rings also get close to each other; the piston cylinder can discharge the seawater in the piston sleeve through the water outlet hole, and the piston rod arranged in the piston sleeve can also discharge the seawater accumulated in the piston cylinder through the second water outlet hole when the piston sleeve and the piston cylinder get close to each other.

The invention discloses a tuna culture strategy optimization method based on a pond culture management platform, and the method comprises the steps: carrying out the monitoring of a target culture pond through a heterogeneous sensor network, obtaining the monitoring information of the culture pond, and carrying out the preprocessing of the monitoring information of the culture pond; based on the preprocessed monitoring information of the aquaculture pond, analyzing the feeding health condition of the aquaculture pond in a stable feeding stage in the target area, and judging whether aquaculture regulation and control need to be carried out or not; on the basis of the preprocessed monitoring information of the aquaculture pond, the tuna adaptation condition of the aquaculture pond in the fishing, transferring and recovering stage in the target area is analyzed, and an optimal transferring and recovering regulation and control strategy is formulated; the abnormal water quality change indexes and conditions after feeding are analyzed, whether the water quality control equipment is abnormal or not is judged when it is detected that the water quality is abnormal, maintenance early warning is carried out, and the comprehensiveness and intelligence of tuna pond culture are improved.

The application relates to application of specific primers in identifying Antarctic krill oil and other oils, and belongs to the field of food chemistry and analysis technology, wherein the primers are F: 5'-GTATTTTGACCGTGCAAAGG-3' and R: 5'-ATAGGGTCTTATCGTCACTT-3', and the other oils are salmon oil, tuna oil, cod oil and soybean oil. The application realizes enrichment of biological information substances in Antarctic krill oil and separation of interference items by using ionic liquid and phase separation technology, so that rapid identification of the Antarctic krill oil is realized.

A feed for farming tuna species includes a body portion flattened with a length in a thickness direction orthogonal to a width direction and to a longitudinal direction, being shorter than a length in the width direction orthogonal to the longitudinal direction. The feed satisfies (A) and / or (B): (A) when a maximum length in the longitudinal direction is defined as a (mm), a maximum length in the width direction is defined as b (mm), and a maximum length in the thickness direction is defined as c (mm), a flattening ratio f1 calculated by Formula (1) is about 110 or greater: f1=a×b / c (1); and (B) when a volume of the feed for fish farming is defined as V (cm3), and an area of the feed for fish farming in a plan view is defined as A (mm2), (V / A)1 / 2 is about 0.110 or less.

The invention relates to the technical field of peptide preparation, and discloses tuna-sourced anserine with a uric acid reducing effect and a preparation method thereof.The preparation method comprises the following steps that back and abdomen meat of fresh tuna is selected and stirred into meat paste, then distilled water is added, water bath heating and cooling are conducted, and then filtering is conducted to obtain filtrate I; protease is added into the filtrate I for enzymolysis, after enzymolysis is completed, inactivation and centrifugation are conducted, and enzymatic hydrolysate is obtained; filtering the enzymatic hydrolysate with an ultrafiltration membrane to obtain a filtrate II; adding a corn stigma extracting solution into the filtrate II, adding a carbon source, a nitrogen source and a protective agent, inoculating strains for fermentation, and after fermentation is completed, filtering and sterilizing to obtain filtrate III; and freeze-drying the filtrate III to obtain the goose carnosine freeze-dried powder. According to the tuna-sourced goose carnosine with the uric acid reducing effect and the preparation method thereof, the xanthine oxidase inhibition rate of the prepared goose carnosine freeze-dried powder is larger than 70%, generation of uric acid is obviously reduced, and the preparation method is simple, safe and beneficial to wide application.

The invention relates to the technical field of aquaculture, in particular to yellow fin tuna soft pellet feed and a domestication and feeding method thereof. The soft pellet feed for the yellow-fin tuna is suitable for the yellow-fin tuna in a land-based culture pond, mainly comprises eel powder, chilled trash fish, spirulina powder, vitamin E, vitamin C, beer yeast and bacillus, and comprises the following nutritional ingredients: 52.17% of crude protein, 9.73% of crude fat, 11.09% of crude ash, 44.7% of water, and the balance of water. The nutritional requirements of the yellow-fin tuna can be completely met. The yellow-fin tuna soft pellet feed prepared by the invention not only has the advantages of good food calling effect and short food domestication time, but also can improve the growth performance and immune function of the yellow-fin tuna, can effectively improve the survival rate of the yellow-fin tuna in land-based culture, and promotes the development of the land-based domesticated yellow-fin tuna.

This invention discloses a biomimetic tuna dorsal fin control method based on policy search. The method includes: controlling the dorsal and caudal fins of the biomimetic tuna based on a policy gradient algorithm; real-time acquisition of power parameters and motion posture parameters of the dorsal and caudal fins during the biomimetic fish's movement; inputting the power parameters and motion posture parameters into a pre-trained reinforcement learning network model and outputting predicted motion parameter spaces for each CPG model; the reinforcement learning network model is constructed based on a policy gradient algorithm; and adjusting the parameter space of the CPG model using the predicted real-time parameter space. This invention can help achieve a more optimized coordinated motion parameter space for the dorsal and caudal fins of the biomimetic tuna.

This invention discloses a specific DNA fragment, primers, identification method, and application for sex identification in yellowfin tuna. The specific DNA fragment has the following gene sequence: male as shown in SEQ ID NO.1 and female as shown in SEQ ID NO.2. Primers were designed based on the specific DNA fragment sequence for PCR amplification and Sanger sequencing analysis. The genotype at position 219 of the male yellowfin tuna DNA fragment is T / G heterozygous, and at position 292 it is A / G heterozygous. The genotype at position 219 of the female yellowfin tuna DNA fragment is T / T homozygous, and at position 292 it is A / A homozygous. This invention successfully achieves non-lethal sex identification during the fry or juvenile stage, which is beneficial for the implementation of asexual reproduction and seedling selection in yellowfin tuna, and has significant practical value and application potential.

The invention discloses a fillet anti-freezing and water-retaining polypeptide and a preparation method and application thereof.The preparation method comprises the steps that fish scales, residual fish meat and fat on tuna skin are removed, deionized water is added for homogenization after the tuna skin is cleaned and cut into pieces, and homogenate is obtained; performing magnetic stirring and centrifugation, taking supernatant liquid, performing centrifugation again, and drying the supernatant liquid to obtain crude protein; and adding deionized water into the crude protein to obtain a crude protein aqueous solution, carrying out enzymolysis, enzyme deactivation, pH adjustment and centrifugation, freeze-drying the supernatant, adding water to redissolve, and adjusting the concentration to 1-3 mg / mL to obtain the tuna skin anti-freezing water-retention polypeptide. The polypeptide can inhibit formation of ice crystals, reduce water loss and degradation of protein structures by the ice crystals, prevent secondary and tertiary structural changes of proteins in a freeze-thaw cycle, inhibit oxidation of myofibrillar proteins, reduce protein denaturation and fat oxidation, effectively guarantee the flavor and water binding capacity of fillets, prevent protein denaturation and improve the frozen quality of salmons.

The invention discloses a tuna bone and meat separation device, and relates to the technical field of fishbone processing mechanical equipment, the tuna bone and meat separation device comprises a fishbone cutting device, a feeding main plate is arranged in the middle of the rear end of the fishbone cutting device, a bone and meat separation device is arranged at the front end of the fishbone cutting device, and a discharging plate is arranged at the front end of the bone and meat separation device; a plurality of supporting legs are arranged at the bottom ends of the bone and meat separating device and the fish bone cutting device. According to the tuna bone and meat separating device, tuna bones can be cut through the two cutting rotary drums which are parallel up and down, the tuna bones can be tidily cut into a plurality of blocks, expansion arc plates can be expanded under the action of an electric push strip when the distance of the cutting rotary drums is changed, the expansion arc plates can be attached to the surfaces of the tuna bones all the time, and therefore the tuna bones can be separated from one another. And the distance between the two cutting rotary drums can be adjusted in a self-adaptive mode through the telescopic adaptive column and the transmission assembly, the self-adaptive capacity is achieved, and the fishbone cutting device can cope with fishbones of different conditions.