83 results about "Thunnus sp." patented technology

The invention discloses a tuna pond culture strategy optimization method and system based on meteorological influence analysis, and the method comprises the steps: collecting regional meteorological data and pond culture monitoring data, and constructing a multi-modal pond ecological feature vector; establishing a weather-water body dynamic coupling model by using a space-time diagram neural network, and generating water body situation response prediction information; a breeding regulation and control strategy is made based on the prediction information, and strategy self-adaptive optimization is achieved through real-time monitoring data; evaluating the anti-disaster capability of the tuna after the meteorological influence is finished, screening high-quality germplasm resources by combining genetic analysis, and generating an anti-disaster breeding scheme. According to the method, full-chain optimization from meteorological early warning to culture regulation and control to genetic breeding is achieved, and the climate toughness and economic benefits of tuna pond culture are comprehensively improved.

Provided herein are altered cell lines comprising a suspension cell line adapted from an adherent cell line having a different expression profile from a corresponding non-altered adherent cell line, methods for generating altered cell lines, and methods of characterizing altered expression profiles for a gene, a transcript, or a protein in an altered cell line.

The invention relates to the field of tuna culture management, and discloses a management method and system for a tuna intelligent pond culture platform, and the method comprises the following steps: collecting the culture associated data of tunas in combination with underwater monitoring equipment, overwater monitoring equipment and water quality monitoring equipment, and constructing a tuna culture evaluation model; carrying out risk assessment on the tuna culture state of the pond based on the tuna culture assessment model; and performing water quality parameter adjustment on the target pond area with the culture risk, and performing visual data display processing through the tuna intelligent pond culture platform. According to the invention, a data monitoring platform can be provided for intelligent culture of tunas, help can be provided for tuna culture more efficiently, the purpose of system automation is achieved, and meanwhile, the harm in the tuna culture process is reduced.

The invention discloses a tuna longline fishing activity prediction method and a tuna longline fishing activity prediction system. The tuna longline fishing activity prediction method comprises the following steps: step S1, obtaining tuna fishing effort data and marine environment data corresponding to the area fishing effort data; s2, constructing space-time sequence tensor data of different space-time scales according to different time resolutions and spatial resolutions; s3, a ConvLSTM-Attention U-Net network model is constructed and trained, an optimal space-time scale model is determined, and the time step length M is larger than or equal to 3; s4, based on the optimal spatial-temporal scale model, visualizing a model prediction result and comparing the model prediction result with real fishing effort distribution; and S5, calculating average absolute gradient values of the variables under different spatial and temporal scales, and evaluating and visualizing the importance of the variables under different spatial and temporal scales. According to the method, the position distribution and the numerical value of the fishing effort can be predicted, and the tuna longline fishing activity in the Chinese and western Pacific Ocean area can be accurately predicted.

The invention relates to a xanthine oxidase inhibitory peptide derived from green and sweet tuna and application of the xanthine oxidase inhibitory peptide, and belongs to the technical field of polypeptides, and the amino acid sequence of the xanthine oxidase inhibitory peptide is Trp-Thr-Ala (WTA), Trp-Val-Pro (WVP), Trp-Leu-Pro (WLP) or Trp-Gly-Phe (WGF). The invention also provides an application of the inhibitory peptide as or in preparation of a xanthine oxidase inhibitor and an application of the inhibitory peptide as or in preparation of a medicine with an effect of inhibiting uric acid level. The invention also provides a xanthine oxidase inhibitor containing at least one inhibitory peptide or a composition or functional food for inhibiting uric acid. The selected inhibitory peptide is a novel food-borne XO inhibitory peptide, is good in water solubility, non-toxic and good in small intestine absorption and blood brain barrier permeability, has the potential of serving as a xanthine oxidase inhibitor and reducing the uric acid level, and can be used for preparing a medicine for preventing and treating hyperuricemia or serving as a functional food additive.

The invention relates to the technical field of aquatic product processing, in particular to an integrated low-loss processing method of tuna fillets. The method comprises the following steps: performing low-temperature radio-frequency quick thawing on cryopreserved tuna fish fillets, taking out the tuna fish fillets until the central temperature of the tuna fish fillets reaches 4 DEG C, and quickly cutting the tuna fish fillets into blocks; soaking the fish blocks in a composite fresh-keeping soaking solution at 3-5 DEG C, and performing low-temperature ultrasonic treatment; and washing the soaked fish blocks, draining the surfaces of the fish blocks, loading the fish blocks into a vacuum packaging bag according to specification requirements, vacuumizing, putting the vacuum packaging bag into a water bath kettle, carrying out temperature gradient type low-temperature curing, and taking out to obtain a cured finished product of the tuna fish fillet. Through combined application of a low-temperature radio-frequency quick thawing technology and a vacuum low-temperature low-loss curing technology, the obtained tuna fillet cured product not only ensures the quality of fish meat, but also effectively inhibits histamine increase and fat oxidation of the cured fish meat during low-temperature storage; the method is of great significance in promoting quality improvement and diversified development of tuna products.

The invention discloses a tuna quality grading intelligent detection method based on multivariate information feature fusion, and relates to the technical field of fish quality grading detection. The method comprises the following steps: acquiring fish body images and meat quality images of a plurality of different tunas, collecting related text information of the tunas, including producing areas, varieties, weights and body lengths, and constructing a data set; preprocessing the image and the related text information; generating a multi-modal fusion feature vector through feature extraction and fusion; inputting into a tuna quality grade detection module; and training the deep learning model, and subsequently performing quality grading detection on the to-be-detected tuna. According to the method, the fish body image, the meat quality image and the related text information are fully considered, the multi-modal fusion features including the fish body, the meat quality and the related text information are generated by adopting the multi-modal feature fusion technology, and training is performed through the quality grading detector, so that the robustness and the accuracy of detection are improved, and the detection result is more comprehensive and reliable.

The invention relates to the technical field of biology, in particular to antioxidative peptide and ACE (angiotensin converting enzyme) inhibitory peptide from tuna as well as a preparation method and application of the antioxidative peptide and the ACE inhibitory peptide. The amino acid sequences of the polypeptide provided by the invention are as shown in SEQ ID NO.1-6. The preparation method comprises the following steps: by taking processing waste tuna skin as a raw material, carrying out enzymolysis to obtain tuna skin collagen, then carrying out liquid chromatography-tandem mass spectrometry identification and molecular docking screening on a tuna skin collagen zymolyte, and extracting to obtain polypeptide with antioxidant activity and polypeptide capable of effectively inhibiting ACE (Angiotensin Converting Enzyme) activity. According to the method, the protein extraction step is simplified; meanwhile, on one hand, the deep processing level of the tuna industry can be improved, high-value utilization is achieved, and on the other hand, the bearing pressure of the environment on the processing waste can be reduced.

The invention relates to the technical field of tuna product processing, and discloses a composite seasoning processing method for reducing the fishy smell of tuna products, which comprises the following steps: trimming, cutting and rinsing tuna raw materials to obtain tuna meat blocks, preparing a composite pickling solution, mixing the tuna meat blocks with the composite pickling solution at low temperature, standing, and then cooling to obtain the tuna products. The preparation method comprises the following steps: pickling tuna meat blocks to obtain pickled tuna meat blocks, carrying out high-temperature shaping treatment on the pickled tuna meat blocks, then carrying out forced cooling treatment to obtain cooled tuna meat blocks, and carrying out low-temperature drying treatment on the cooled tuna meat blocks to obtain the tuna product. The method solves the problems that when high-temperature baking is adopted in an existing tuna processing technology, the outer part is scorched and the inner part is generated due to uneven heating power, and the product quality is unstable and the taste is poor due to the fact that fishy smell substance residues cannot be fundamentally eliminated by depending on a traditional pickling method.

The invention discloses an goose carnosine extraction preparation process and application thereof, and belongs to the technical field of goose carnosine extraction.The goose carnosine extraction preparation process comprises the following steps that firstly, tuna bones and bone marrow are separated, and pretreatment is conducted; 2, ultrasonic high-speed stirring treatment of tuna bone meal and bone marrow; 3, heat extraction; 4, membrane separation; and 5, mixing the target peptide with the liquid in the step 1, and then drying to obtain the goose carnosine with high stability. According to the method for extracting the tuna goose carnosine, the extraction rate is higher than 78%, the purine removal rate is higher than 55%, and the DPPH free radical scavenging rate is higher than 75%. The stabilizing agent composed of the malic acid, the betaine and the rhamnose not only can perform acidification and fermentation pretreatment on tuna bones, but also can play a role in stabilizing an goose carnosine sample, meanwhile, the extraction rate of tuna goose carnosine is finally improved, and the purine content can be effectively reduced by combining membrane filtration. High-voltage current treatment is performed before membrane separation, so that the DPPH free radical scavenging rate of the tuna goose carnosine can be increased.

The utility model discloses ground fish sterilization equipment for tuna, and relates to the technical field of tuna sterilization equipment, the ground fish sterilization equipment comprises a bottom plate, the upper side of the bottom plate is provided with a moving groove, the inner wall of the moving groove is rotatably matched with a corrosion-resistant screw rod, the corrosion-resistant screw rod is in threaded fit with a moving block, and the upper side of the moving block is provided with a movable groove. The guide plates and the trigger blocks are arranged, so that in the moving process of the placing net frame, after the guide plate on the lower side is in contact with and abuts against the trigger blocks, extrusion with the corresponding frequency can be generated on the placing net frame, under the condition of tension of the tension springs, the placing net frame vibrates up and down, and therefore fish blocks on the placing net frame are vibrated; the water on the surface of an object is removed through the sterilization lamp tube, the influence on subsequent sterilization is avoided, the subsequent sterilization effect is improved, and after the placing net frame is moved into the sterilization box, secondary sterilization is performed on the fish meat through the irradiation of the sterilization lamp tube, so that the sterilization comprehensiveness is improved.

The invention provides traditional Chinese medicine powder for improving the transfer survival rate of yellow fin tuna, which is prepared by weighing the following components in percentage by mass: 15-20% of radix bupleuri, 10-15% of radix puerariae, 15-20% of liquorice, 5-10% of Chinese herbaceous peony, 5-10% of blackberry lily, 5-10% of fructus forsythiae, 5-10% of poria cocos, 5-10% of rhizoma anemarrhenae, 5-10% of pericarpium citri reticulatae and 20-25% of gypsum, totaling 100%. All the materials pass through a 200-mesh screen and are uniformly mixed to obtain the traditional Chinese medicine powder for improving the transfer survival rate of the yellow fin tuna. The traditional Chinese medicine powder takes common Chinese herbal medicines as main raw materials, has the characteristics of rich resources, wide raw material sources, easiness in obtaining and the like, is free of toxic and side effects, free of hormones, difficult to pollute the environment, free of residues in the bodies of the yellow fin tunas, safe to use, simple to operate, low in cost and suitable for popularization, and has a wide application prospect. The survival rate of the yellow fin tuna in the transportation process can be increased, the immunity of the fish body can be adjusted, and the anti-stress capability of the fish body is enhanced.

The invention provides a method for extracting goose carnosine and / or carnosine. The method comprises the steps of low-salt water extraction and high-salt water extraction under alkaline and heating conditions. The extraction method of the goose carnosine and / or carnosine provided by the invention is simple to operate, low in cost and easy for large-scale industrial use.

The invention discloses a preparation process of a normal-temperature instant seasoning tuna neck product. The preparation process comprises the following steps: (1) pretreating raw materials; (2) pickling; (3) secondary low-temperature baking; and (4) bagging and sterilizing. According to the preparation technology of the normal-temperature instant seasoning tuna neck product, the requirement for equipment is low, the technology steps are simple, operability is high, the prepared normal-temperature instant seasoning tuna neck product is good in flavor and appearance quality and convenient to carry and eat, the variety of tuna neck products is enriched, and the taste of the tuna neck products is improved. A new way is provided for deep processing of the tuna necks, and the economic value of the tuna necks can be improved.

The invention relates to application of specific primers in identification of euphausia superba oil and other oils and fats, and belongs to the technical field of food chemistry and analysis, the primers are F: 5 '-GTATTTTGACCGTGCAAAGG-3' and R: 5 '-ATAGGGTCTTATCGTCACCT-3', and the other oils and fats are salmon, tuna, cod and soybean oils and fats. According to the method, the enrichment of biological information substances in the euphausia superba oil and the separation of interference terms are realized by utilizing ionic liquid and a phase separation technology, so that the rapid identification of the euphausia superba oil is realized.

The present application relates to the technical field of biology, and particularly relates to an antioxidant peptide and ACE inhibitory peptide from tuna, and a preparation method and application thereof. The amino acid sequence of the polypeptide provided by the present application is shown as SEQ ID NO. 1-6. The present application takes tuna skin waste as raw material, obtains tuna skin collagen through enzymolysis, then performs liquid chromatography-tandem mass spectrometry identification and molecular docking screening on the tuna skin collagen enzymolysis product, and obtains polypeptides with antioxidant activity and polypeptides effectively inhibiting ACE activity. The method simplifies the protein extraction step. Meanwhile, the method can improve the deep processing level of the tuna industry, achieve high-value utilization, and reduce the environmental pressure on processing waste.

This invention provides a method for preparing tuna skin collagen peptide zinc ion chelate, belonging to the field of marine biological functional product processing. The method mainly involves hydrolyzing tuna skin using a complex protease to obtain collagen active peptides, and then chelating these active peptides with a zinc ion solution to finally obtain the tuna skin collagen peptide zinc ion chelate. The preparation method provided by this invention is simple and convenient to operate, low in cost, and has good safety. The yield of the chelate prepared by this invention is as high as 70% or more, the chelation rate is over 85%, and the molecular weight distribution of the chelate has a certain regularity, with the mass percentage of molecules with a molecular weight distribution of 3-5 kDa exceeding 49%. The chelate prepared by this invention, with active peptides chelated with zinc ions, can exert a synergistic effect, not only enhancing the biological activity of the original polypeptide components but also greatly reducing the toxic side effects caused by direct zinc ion supplementation.

The utility model discloses a freezing device for tuna preservation, and relates to the technical field of tuna freezing. The tank comprises a bottom plate and a tank body fixedly mounted at the top of the bottom plate, a driving mechanism and an overturning mechanism are arranged on the bottom plate, the driving mechanism comprises a motor groove formed in the top of the bottom plate, a motor is fixedly mounted on the inner wall of the bottom of the motor groove, a rotating shaft is fixedly mounted on an output shaft of the motor, and the rotating shaft is fixedly mounted on the rotating shaft. And the top end of the rotating shaft extends into the tank body and is rotationally connected with the tank body, a rotating disc is fixedly installed at the top of the rotating shaft, and the outer wall of the rotating shaft is fixedly sleeved with a large gear. According to the tuna fixing device, the driving mechanism is arranged, the three limiting plates are synchronously moved outwards, in the outward moving process of the three limiting plates, the springs are compressed, tunas are placed among the three limiting plates, after the tunas are placed, the three limiting plates are pushed by the springs to get close inwards to fix the tunas, and the tunas are fixed through cooperation of the limiting plates and the springs.

The invention discloses a tuna red meat immunity enhancing peptide and application thereof in enhancing immunity, and belongs to the technical field of biology. According to the invention, polypeptides with immunocompetence are extracted from tuna red meat, and the polypeptides comprise FLQGF and LAKFPDF. Experiments prove that the polypeptide prepared by the invention has no obvious toxic effect on cells, and can improve the proliferation rate and the phagocytic rate of macrophages and the release rate of NO to different degrees. An in-vitro immunomodulatory activity result shows that the LAKFPDF and the FLQGF can activate a TLR4 dependent NF-kB / MAPK signal channel, regulate and control the immune response of macrophages and play an immunomodulatory role. Research results of the invention provide a basis for high-value development and utilization of tuna red meat, and provide a new resource for preparation of immunopotentiation products.

This is a large-fish insulated bag for transporting and storing large fish, such as tuna, while keeping them cool. It measures 2550 mm wide and 1550 mm long in the front view. As shown in the reference oblique view, this bag is formed as a single sheet and is used by folding it in half along the fold line indicated by the dashed line in the figure. This bag is made of two overlapping, flexible, and waterproof layers of fabric, sandwiched between them and a highly insulating material such as urethane. When in use, the left, right, and top edges are sealed with a series of waterproof zippers, preventing water leakage from the interior. Four water taps are provided on the front for releasing water from the interior. As shown in the reference oblique view, the unfolded bag can be placed halfway (upper right or lower left of the fold line) and folded back as described above. The bag can then be placed inside the bag by closing the waterproof zipper.

The invention relates to the technical field of food additives, in particular to tuna bone essence as well as a preparation method and application thereof. The preparation method of the tuna bone essence comprises the following steps: crushing to obtain tuna bone liquid; performing high-pressure cooking: performing high-pressure cooking on the tuna aggregate liquid, discarding waste residues, and taking supernate; performing enzymolysis reaction, namely performing enzymolysis reaction on the supernate twice to obtain tuna bone enzymolysis liquid; fermenting and deodorizing: adding saccharomyces cerevisiae into the tuna bone enzymatic hydrolysate, and fermenting to obtain tuna bone fermentation liquor; and performing Maillard reaction: adding D-xylose, L-cysteine and taurine into the tuna bone fermentation liquid, adjusting the pH value to 7.5-8.5, heating to 110-130 DEG C, and keeping the temperature for 40-60 minutes to obtain the tuna bone essence. According to the preparation method of the tuna bone essence provided by the invention, the flavor of tuna bones cannot be shielded, and the flavor of the tuna bones is improved.

The invention provides iron binding peptides based on tuna blood combined meat. The amino acid sequences of some polypeptides are SEQ ID NO: 1-6. The polypeptide provided by the invention can be used for preparing a product for treating iron-deficiency anemia. According to the tuna red meat hematopoietin provided by the invention, an organic iron compound with high bioavailability is formed through the chelation of specific polypeptide iron ions in preparation of a blood replenishing product, so that the absorption efficiency of intestinal tracts on iron is remarkably improved. A carrier formed by the polypeptide can protect iron ions from being damaged by gastric acid, and the iron ions are delivered to an absorption part in a targeted mode, so that the hemoglobin synthesis efficiency is improved by 40% or above. The natural polypeptide ligand disclosed by the invention avoids gastrointestinal irritation of a traditional iron supplementing agent, and is not interfered by absorption of dietary factors such as phytic acid and tannin. The tuna processing by-product is used as the raw material to prepare the functional blood-enriching peptide, so that high-value utilization of fishery resources is realized, and an eco-friendly new iron-enriching source is provided.

Provided herein are altered cell lines comprising a suspension cell line adapted from an adherent cell line having a different expression profile from a corresponding non-altered adherent cell line, methods for generating altered cell lines, and methods of characterizing altered expression profiles for a gene, a transcript, or a protein in an altered cell line.

The utility model discloses a tuna blood collecting device, and relates to the technical field of tuna processing. The device comprises a bottom plate, the top face of the bottom plate is fixedly connected with a blood collecting groove used for storing fish blood, the surface of the blood collecting groove is fixedly connected with a fixing frame, a tuna hanging assembly is installed on the surface of the fixing frame, and the surface of the fixing frame is further provided with a lifting control assembly used for controlling the hanging height of the tuna hanging assembly. A fixing box is fixedly connected to the right side of the top face of the bottom plate, an automatic collecting assembly used for guiding out fish blood in a blood collecting groove is arranged in the fixing box, the tuna hanging assembly comprises a fixed pulley, the fixed pulley is fixedly installed on the surface of a fixing frame, and a traction rope is slidably connected to the surface of the fixed pulley. The tuna blood collection device has the advantages that tuna blood can be effectively collected during use, stable collection can be realized in a shaking environment of a fishing boat, collection work is more convenient, and practicality is better.

An in-vitro tissue model for use with a test article includes a tissue sample that includes homogeneous tuna meat having an isotropic thermal response, wherein the tuna meat is characterized by a plurality of visually distinct color states, each color state corresponding to a respective temperature to which the tuna meat has been heated within a range of 43° C. to 60° C., thereby providing a visual indication of the thermal effect of the test article within said range.

This invention belongs to the field of functional marine food processing technology, specifically involving a method for producing a composite sausage made from Antarctic krill, tuna, and collagen, comprising the following steps: (1) preparation of Antarctic krill meat, (2) preparation of tuna blood-infused meat, (3) preparation of low-temperature raw pork skin collagen paste, (4) preparation of krill-derived composite enzyme solution, (5) mixing of meat materials and low-temperature enzymatic hydrolysis, (6) low-temperature chopping, (7) sausage filling and quick-freezing, and (8) thawing and maturation of the product. This invention uses Antarctic krill meat, tuna blood-infused meat, and low-temperature raw pork skin collagen paste as core raw materials, and utilizes krill endogenous composite enzymes for low-temperature enzymatic hydrolysis to produce a composite sausage with uric acid-lowering function, high protein content, and high unsaturated fatty acid content, providing a safe, convenient, and daily-consumable functional marine food for people with hyperuricemia.

The invention discloses a rapid fermentation method based on tuna protein sludge. The rapid fermentation method comprises the following steps: S1, uniformly mixing the tuna protein sludge with a solid carbon source to prepare a substrate; s2, at least one synergist is added, and the synergist comprises a, rhamnolipid fermentation liquor with the concentration of at least 3%, the rhamnolipid fermentation liquor is fermentation liquor which is maturely fermented and is not sterilized, and the rhamnolipid fermentation liquor is added according to the weight ratio of 2-5% of the substrate; b, fish protein enzymatic hydrolysate with the concentration of at least 12%, wherein the fish protein enzymatic hydrolysate is added according to the weight ratio of 3-5% of the substrate; c, combination of neutral protease, cellulase and ligninase, wherein 1-30 thousand units of neutral protease, 2-50 thousand units of cellulase and 2-50 thousand units of ligninase are added into each ton of substrate; s3, inoculating a fermentation strain; and S4, building a heap and fermenting. Compared with conventional fermentation, the fermentation method has the advantages that the synergist is added during inoculation, so that the fermentation period is greatly shortened, the investment of equipment and sites is reduced, and the economic benefit is improved.

The invention discloses a strain of Enterococcus faecalis HY0110 and its application in the fermentation of American cockroaches. The strain is isolated and screened from the intestinal contents of bluefin tuna (Thunnus thynnus) collected from the South China Sea, is non-hemolytic, sensitive to a variety of common antibiotics, has strong adaptability to the gastrointestinal environment of animals, has a strong ability to inhibit a variety of digestive tract pathogens, and has a significant growth inhibition effect and a cell apoptosis-promoting effect on human colon cancer cells; the strain is used to ferment American cockroach powder, and the content of amino acids and peptides, astragaloside IV, eicosanoids and other bioactive substances in the supernatant is significantly increased, and has a good symptom relief effect on the DSS mouse colitis model, and the levels of a variety of inflammatory factors are reduced, which opens up a new way for the in-depth development of American cockroaches.

The invention provides a combined unfreezing device suitable for tuna and a method thereof, and relates to the technical field of tuna processing. The combined unfreezing device suitable for tunas comprises an unfreezing room, a refrigerating box, an unfreezing machine, a high-frequency vibration device, an infrared heating device and a low-temperature vacuum device are installed in the unfreezing room, the refrigerating box is installed in the unfreezing room, and the unfreezing machine comprises an installation frame. And the upper surface of the mounting frame is fixedly connected with a placement box. The method comprises the following steps: firstly, moving cold tunas into an unfreezing room, then placing the tunas in a refrigeration box for a period of time, so that the tunas are slightly unfrozen, then placing the tunas in a placing frame, starting an electromagnetic heating device, and unfreezing the tunas by the electromagnetic heating device. The temperature of each place of the tuna is detected in real time through the temperature sensor, and the effect of preventing excessive water loss and protein denaturation of the tuna is achieved through the design.

The invention relates to the technical field of fishery. A small tuna on-ship temporary rearing device comprises a temporary rearing fishpond, the temporary rearing fishpond comprises a water inlet pond on the front side, a fish storage box in the middle and a fish releasing pond on the rear side, and a guiding device is arranged in the temporary rearing fishpond; the fish storage box is of a multi-layer structure, all layers of the fish storage box are separated through first partition plates, water permeable holes are formed in the surfaces of the first partition plates, and a plurality of limiting devices used for limiting tuna movement are arranged on each layer of the fish storage box at intervals. The invention provides a device for temporarily rearing small tunas on a ship, which can effectively pretreat the conditions of spalling of mucus layers and body surface scratching of small tunas in the process of temporarily rearing the small tunas on the ship and increase the survival rate and the survival state of the small tunas in the process of temporarily rearing the small tunas on the ship. The technical problems that in the prior art, an existing small tuna on-ship temporary rearing device is single, collision is likely to happen in the on-ship temporary rearing process, a surface mucus layer is likely to fall off, the body surface is likely to be scratched, and the survival rate and the survival state are not ideal enough are solved.