Tuna peptide, extraction method thereof and application of tuna peptide as antihypertensive agent

An extraction method, tuna technology, applied in the fields of application, fish protein components, medical preparations containing active ingredients, etc., can solve the problems of increased processing difficulty, accompanying fishy smell, unsatisfactory purity of tuna peptides, etc.

Inactive Publication Date: 2020-07-17
荣成市慧海创达生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the inventor believes that the purity of the tuna peptide obtained by the existing extraction method is not ideal, and ...

Method used

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  • Tuna peptide, extraction method thereof and application of tuna peptide as antihypertensive agent
  • Tuna peptide, extraction method thereof and application of tuna peptide as antihypertensive agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] 1) Raw material homogenization and crushing: wash the tuna with clean water, remove internal organs and sediment, then add a certain amount of distilled water and stir evenly, and process it with a high-pressure homogenizer for 1 hour to obtain a homogenate.

[0050] 2) Fermentation and enzymatic hydrolysis: dilute to 10% solids concentration with culture medium, add 5% compound protease to the homogenate, inoculate Candida tropicalis (10%) at the same time, and ferment and enzymatically hydrolyze at 30°C for 72 hours.

[0051] 3) Centrifugation and high-temperature sterilization: high-temperature sterilization of the above-mentioned solution for 10-60 minutes, and centrifugation at 9000 rpm for 15-20 minutes after cooling.

[0052]4) Resin adsorption refining: put the concentrated absorption solution on the column equipped with macroporous adsorption resin, the amount of sample loading is the absorption solution equivalent to the weight of the resin; the flow rate of sa...

Embodiment 2

[0055] 1) Raw material homogenization and crushing: wash the tuna with clean water, remove internal organs and sediment, then add a certain amount of distilled water and stir evenly, and process it with a high-pressure homogenizer for 1 hour to obtain a homogenate.

[0056] 2) Fermentation and enzymolysis: Dilute with culture medium to a solid concentration of 8%, add 3% compound protease to the homogenate, inoculate Candida tropicalis at the same time, and ferment and enzymolyze at 35°C for 48 hours.

[0057] 3) Centrifugation and high-temperature sterilization: high-temperature sterilization of the above-mentioned solution for 10-60 minutes, and centrifugation at 6000°C for 15-20 minutes after cooling.

[0058] 4) Resin adsorption refining: put the concentrated absorption solution on the column equipped with macroporous adsorption resin, the amount of sample loading is the absorption solution equivalent to the weight of the resin; the flow rate of sample loading is controlled...

Embodiment 3

[0061] 1) Raw material homogenization and crushing: wash the tuna with clean water, remove internal organs and sediment, then add a certain amount of distilled water and stir evenly, and process it with a high-pressure homogenizer for 1 hour to obtain a homogenate.

[0062] 2) Fermentation and enzymolysis: Dilute with culture medium to a solid concentration of 15%, add 2% compound protease to the homogenate, inoculate Candida tropicalis at the same time, and ferment and enzymolyze at 25°C for 120 hours.

[0063] 3) Centrifugation and high-temperature sterilization: high-temperature sterilization of the above-mentioned solution for 10-60 minutes, and centrifugation at 5000-9000 rpm for 15-20 minutes after cooling.

[0064] 4) Resin adsorption refining: put the concentrated absorption solution on the column equipped with macroporous adsorption resin, the amount of sample loading is the absorption solution equivalent to the weight of the resin; the flow rate of sample loading is c...

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Abstract

The invention particularly relates to tuna peptide, an extraction method of the tuna peptide and an application of the tuna peptide as an antihypertensive agent. The tuna peptide is a component with agood health-care effect, and extraction of the tuna peptide from leftovers of tuna processing products is a resource utilization mode with remarkable economic significance. Tuna peptide extracted bya traditional preparation method often has the defects of poor purity, poor sense and the like. The invention provides a treatment method for fermentation and enzymolysis by adopting microorganisms and compound protease at the same time, polypeptide is fully released from leftover protein hydrolysate of tuna production, the extraction efficiency is improved, resin adsorption is combined, and the method has the advantages of no fishy smell, environmental friendliness, a high extraction rate, high purity and the like.

Description

technical field [0001] The invention belongs to the technical field of protein polypeptide extraction methods, and in particular relates to a tuna peptide, a method for extracting the tuna peptide and its application as an antihypertensive active ingredient in the preparation of antihypertensive preparations. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] The crude protein content of yellowfin tuna back muscle is 26.2%. The fat content is only 0.2%, which is a high-protein, low-fat healthy food; according to the essential amino acid requirement model (adult model) recommended by FAO / WHO in 1973, the back muscle protein of yellowfin tuna is evaluated, and the flavor amino...

Claims

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Application Information

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IPC IPC(8): A23J1/04A23J3/04A23J3/34A23L33/00A61K38/01A61P9/12
CPCA23J1/04A23J3/04A23J3/341A23L33/00A23V2002/00A61K38/012A61P9/12A23V2200/326A23V2250/2042A23V2250/55
Inventor 方诩韩丽娟贾洋洋
Owner 荣成市慧海创达生物科技有限公司
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