Compound enzyme-microwave extraction method for optimizing Nitraria polysaccharide by response surface methodology

A technology of response surface method and white thorn polysaccharide, which is applied in the biological field to achieve the effect of increasing polysaccharide content, saving extraction time, and avoiding potential safety hazards.

Active Publication Date: 2015-10-21
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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Problems solved by technology

There is no patent application for complex enzyme-microwave-assisted extraction of Nitraria anti-aging active polysaccharides

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  • Compound enzyme-microwave extraction method for optimizing Nitraria polysaccharide by response surface methodology
  • Compound enzyme-microwave extraction method for optimizing Nitraria polysaccharide by response surface methodology
  • Compound enzyme-microwave extraction method for optimizing Nitraria polysaccharide by response surface methodology

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Embodiment Construction

[0089] A compound enzyme microwave extraction method utilizing response surface method to optimize Nitraria polysaccharide, comprising the following steps:

[0090] ⑴Sample preparation:

[0091] Wash the fresh Nitraria fruit, dry it at 30-35°C for 48-72 hours, and then crush it to 40-80 mesh. Soak at ℃~25℃ for 2~4h and then filter to obtain the filter residue, which is dried at 40~60℃ for 12~24h to obtain the white thorn fruit powder from which lipids, pigments and small molecular sugars have been removed; then in a microwave reactor , according to the different solid-liquid ratio of 10~18 mL / g, add the compound enzyme solution, stand and soak at 40~50℃ for different times of 1.5~2.5h, and at different microwave powers of 330~370W, according to different times of 25~35 min Perform reaction extraction, and the extracted solution is centrifuged at 4000~5000 rpm, and the supernatant A is collected after 15~20 minutes, and the supernatant A is concentrated to 1 / 6 of the original ...

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Abstract

The invention relates to a compound enzyme-microwave extraction method for optimizing Nitraria polysaccharide by a response surface methodology. The method comprises the following steps: (1) carrying out sample preparation: preparing Nitraria fruit powder from fresh Nitraria fruits; then, carrying out reaction extraction according to different material / liquid ratios, different soaking times, different microwave powers and different extracting times, so as to obtain dried Nitraria polysaccharide extract powder corresponding to different microwave powers; (2) determining the content of polysaccharides in each extract by using a phenol-sulfuric acid method; (3) carrying out experimental design and statistical analysis: 1) carrying out single-factor test; and 2) carrying out response surface method optimization design: establishing a multivariate quadratic regression equation by selecting four factors, i.e., microwave power, extracting time, soaking time and material / liquid ratio, which have relatively remarkable influence on polysaccharide extracting effect, according to single-factor test results; and (4) analyzing and optimizing experimental results: carrying out drawing analysis according to the multivariate quadratic regression equation by using software Design Expert 8.0, so as to obtain a response surface of the regression equation and a contour map of the response surface. The method is simple in operation and high in extraction ratio.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a compound enzyme microwave extraction method using response surface method to optimize Nitraria polysaccharide. Background technique [0002] Tribulus terrestris ( Nitraria ) plant is an ancient small genus, xerophytic or super-xerophytic shrubs, small shrubs, 12 species have been found in the world, and 8 species in my country, mainly distributed in Qinghai, Xinjiang, Gansu, Ningxia, Inner Mongolia and other places, the resources are very rich . Among them Nitraria tangulata ( N. tangutorum ) and Siberian Nitraria ( N. sibirica ) is a traditional medicinal material of Mongolian, Tibetan, Uighur and other ethnic minorities in the west. It is used for the treatment of various diseases and often appears in Chinese and Tibetan medicine compound prescriptions. Modern pharmacological studies have shown that Nitraria fruit extract has the effects of lowering blood fat, anti-oxidation...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 毕宏涛魏立新高婷婷杜玉枝杨红霞李岑
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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