Compound enzyme-microwave extraction method for optimizing Nitraria polysaccharide by response surface methodology
A technology of response surface method and white thorn polysaccharide, which is applied in the biological field to achieve the effect of increasing polysaccharide content, saving extraction time, and avoiding potential safety hazards.
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[0089] A compound enzyme microwave extraction method utilizing response surface method to optimize Nitraria polysaccharide, comprising the following steps:
[0090] ⑴Sample preparation:
[0091] Wash the fresh Nitraria fruit, dry it at 30-35°C for 48-72 hours, and then crush it to 40-80 mesh. Soak at ℃~25℃ for 2~4h and then filter to obtain the filter residue, which is dried at 40~60℃ for 12~24h to obtain the white thorn fruit powder from which lipids, pigments and small molecular sugars have been removed; then in a microwave reactor , according to the different solid-liquid ratio of 10~18 mL / g, add the compound enzyme solution, stand and soak at 40~50℃ for different times of 1.5~2.5h, and at different microwave powers of 330~370W, according to different times of 25~35 min Perform reaction extraction, and the extracted solution is centrifuged at 4000~5000 rpm, and the supernatant A is collected after 15~20 minutes, and the supernatant A is concentrated to 1 / 6 of the original ...
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