517results about How to "High in polysaccharides" patented technology

The present invention relates to a tissue culture production technology and a cultivation method of dendrobium nobile, which include the following steps: inserting seeds of dendrobium nobile into a culture medium of N6 + NAA 0.3mg/L + potato juice 20%(V/V) + agar-agar 5g/L to perform cultivation, at 22-25 DEG C, for 10-12 hours of average diurnal light, under 1800-2500lx intensity of illumination, for 15-30 days; transplanting the cultivated seeds to a culture medium of N6 + BA 0.2mg/L + potato juice 20%(V/V) + agar-agar 5g/L to perform cultivation for 25 days; transplanting the cultivated seeds to a culture medium of N6 + NAA 0.2mg/L + banana juice 10%(V/V) + potato juice 20%(V/V) + agar-agar 6g/L for two months to form big seedlings; planting the big seedlings in wood chips which mixed blue stones and decomposed organic fertilizers for hardening-seedling, which begins in every March for one year, and spraying water in time; and transplanting the hardened seedlings to open ground or greenhouse to perform cultivation with a transplanting medium of shale + moss and to fasten the seedlings by cable fasteners, under appropriate humiture and light irradiation, managing with GAP qualitative specification.

The invention relates to an extracting method for an active polysaccharide in a higher plant or edible and medicinal fungi. The extracting method comprises the following steps: adding an organic acid or an organic or inorganic mixed acid solution in raw materials; heating the raw materials, utilizing the molecules of the organic acid to separate the active polysaccharide from the raw materials, and moderately cutting off glucosidic bonds of macromolecular active polysaccharide of the plant, thereby realizing local degradation; adopting a distilling, filtering or centrifuging method to remove most acid solution in the raw materials, and then washing with an organic solvent, thereby removing few remained acid solutions; extracting by adding water with the volume being 5-15 times that of theraw materials into the raw materials processed with the organic acid, and after concentrating an extracting solution, settling alcohol, thereby obtaining rough active polysaccharide; dissolving the rough active polysaccharide in de-ionized water with the volume being 5-15 times that of the rough active polysaccharide; adjusting pH of the solution to be neutral; and centrifuging, dialyzing or film-separating liquid supernatant, and then concentrating and drying, thereby obtaining an active polysaccharide product with low average molecular weight, narrow molecular weight distribution of the polysaccharide and excellent water solubility. Various defects, such as water consumption and energy consumption caused by technology, low yield of products, and the like, are overcome.

The invention provides a ganoderma lucidum mycelia powder production process which comprises the following steps of: (1) making grain culture mediums; (2) preparing liquid strains; (3) inoculating the grain culture mediums; (4) sending bottles with inoculated strains into a mycelia growth room; (5) sending the bottles into a bottle digging workshop after the bottles of culture mediums are full of mycelia and digging the mycelia out of the bottles; (7) drying the mycelia in a hot air dryer; (7) grinding the dried corn mycelia, wheat mycelia and soybean mycelia into coarse powder in a proportion of 58-62%: 34-36%: 4-6%; (8) placing the ground coarse powder into a mixing agitator, adding water to agitate evenly and puffing after the water content is up to 10%, wherein the puffing temperature is controlled within 109-111 DEG C; and (9) sending the puffed granular mycelia into an ultra-micro grinder to grind into 150-mesh powder, and packing to obtain finished products. The ganoderma lucidum mycelia powder produced by the method is good in taste and easy to digest and has the advantages of adjusting the immunity and improving the anti-hypoxia capability of organisms.

The present invention relates to a method for extracting ganoderic spore polysaccharide. It is characterized by that firstly, it uses CO2 supercritical extraction method to extract ganoderic spore oil for another application, and makes the residue obtained after the ganoderic spore oil is extracted undergo the processes of removing fat, water extraction, alcohol precipitation, filtration and drying so as to obtain the invented ganoderic spore polysaccharide.

The invention discloses an enzymatic extraction method for auricularia auricula polysaccharides, which comprises the following steps of: mechanically and coarsely grinding dried auricularia auricula, sieving, mixing according to a material-to-water ratio of 1:(50-200), and extracting at the temperature of between 35 and 50 DEG C and the pH of between 5.0 and 9.0 under the action of chitinase for 60 to 180 minutes, wherein the addition amount of the chitinase is 100 to 600U/g; raising the extraction temperature to be between 80 and 95 DEG C, extracting for 60 to 180 minutes to obtain extract, and centrifuging to obtain supernatant and precipitates; and adding ethanol into the supernatant to precipitate polysaccharides, performing centrifugal separation to obtain precipitates, dissolving the precipitates in water, concentrating to remove residual ethanol, adding a mixture of diatomite and activated carbon into concentrated solution, mixing, fully stirring, filtering, performing membrane filtration on filtrate by using a polysulfone membrane of 30 to 100kD, collecting macromolecular concentrated solution which does not filter through the membrane, and drying the macromolecular concentrated solution to obtain the auricularia auricula polysaccharides. The prepared products have high purity and bioactivity; and the polysaccharide content is over 40 percent.

The invention discloses a method for extracting polysaccharide of dendrobium officinale. The method specifically comprises the following steps of uniformly mixing dendrobium officinale powder and a deep eutectic solvent according to a material and liquid ratio of 1g to (30 to 50)mL, wherein the ratio of hydrogen bond receptor to hydrogen bond donor is 3 to (1 to 18)mol/mol; adding 4 to 8mg/mL of at least one of cellulase and pectinase; extracting for 90 to 120min at the temperature of 30 to 60 DEG C; separating and purifying the extracting solution, so as to obtain the polysaccharide of dendrobium officinale. Compared with the traditional enzyme type water extracting method, the method has the advantages that by using the enzyme-assisted deep eutectic solvent to extract, the volatility islow, the stability is high, and the reaction system is mild; the content of impurities in the extracting solution is reduced, the loss of the solvent is reduced, the purifying burden is decreased, theextracting rate of polysaccharide is increased by 10% or above, and the content of polysaccharide is increased by 10% or above.

The invention relates to cordyceps militaris active polysaccharide prepared by a method comprising the following steps: performing ultrafine grinding on cordyceps militaris sporophores, then extracting with boiling water, and collecting supernatant fluid; and concentrating the supernatant fluid, then adding ethanol for deposition, passing a deposited part through a DEAE-sepharose column, desalting a part of 0-0.5N, and then performing freeze-drying. The invention also relates to an application of a cordyceps militaris extract in preparation of an anti-tumor activity product.

The invention discloses a hericium erinaceus liquid fermented mash beverage and a preparation method thereof. A special strain of the hericium erinaceus liquid fermented mash is prepared from a culture medium containing a liquorice matrix, wherein the culture medium of the hericium erinaceus liquid fermented mash contains jujube, malt, chrysanthemum, medlar, corn flour and/or soybean cake powder. Every 100mL of the hericium erinaceus liquid fermented mash contains no less than 25g of fresh hericium erinaceus mycelia. Moreover, the hericium erinaceus liquid fermented mash beverage contains hericium erinaceus fruiting body water extract, wherein a weight ratio of the hericium erinaceus liquid fermented mash to the hericium erinaceus fruiting body water extract is 4: 1. The hericium erinaceus liquid fermented mash beverage disclosed by the invention is rich in nutritional ingredient, and the polysaccharide content in the hericium erinaceus mycelia obtained from fermentation is apparently higher than that in a hericium erinaceus fruiting body. The hericium erinaceus beverage preparation method is short in cycle, high in output and low in cost, and has an actual application value.

The invention discloses an extraction and purification method of bioactive polysaccharides in dendrobium huoshanense. The method comprises the following steps: crushing the dendrobium huoshanense, then adding water, performing microwave constant-temperature extraction, and performing vacuum concentration on extract solution till the relative density is 110-1.30 so as to get concentrated solution;adding ethanol into the concentrated solution, standing, and further performing centrifugation to get precipitate; adding the water into the precipitate for dissolution, enabling the solution to passthrough an ultrafiltration membrane in the molecular weight of 100KD under the pressure of 0.35MPa, collecting trapped fluid, then enabling the trapped fluid to pass through the ultrafiltration membrane in the molecular weight of 300KD under the pressure of 0.35MPa, and collecting passing fluid; and performing the vacuum concentration on the collected passing fluid, and drying to get a polysaccharide extract from the dendrobium huoshanense. By adopting the method, the extraction time can be shorted, and the yield of a crude extract can be improved from 20% to about 35% under the same extraction times; and the yield of the polysaccharides from the dendrobium huoshanense after purification can be improved from about 5% to 10-15%, and the content of the polysaccharides from the dendrobium huoshanense can be improved from 40% to above 60%.

A process for preparing the crude polyose from hedgehog fungus by semi-bionic extracting method features that the semi-bionic extraction instead of water extraction and alcohol deposition is used, and the flocculating deposition is used to remove viscose impurities and residual heavy metal, resulting in higher output rate.

The present invention discloses a method tobextract polysaccharide from the jujube. The extraction process is of the following steps: (A) jujube is mixed with water and is extracted for 1 to three times; then the jujube experiences the centrifugal separation and vacuum low-pressure concentration; (B) clarifying agent is added into the jujube mass which is statically placed for centrifugal separation after stirring; (C) the separated sypernatant is added with alcohol, which is more than 70 percent in volume; the alcohol in the sypernatant is deposited overnight, and then the sypernatant experiences centrifugal separation; (D) the solid obtained is dried to get jujube polysaccharides. The jujube polysaccharides obtained through the present invention is more than 40 percent of the original jujube. The whole extraction and separation process involves no toxic and hazardous organic solvents. The present invention is safe and non-toxic. The operation is simple and the consumed time is short. A full green extraction can be achieved through the present invention.

The invention relates to a usage of Brassica rapa polysaccharide which is extracted from Brassica rapa plants in the preparation of anti-hypoxia drugs, the average molecular weight of the Brassica rapa polysaccharide is 30,000-80,000, and the total polysaccharide content is 60-95 percent by weight. The Brassica rapa polysaccharide can be prepared into pharmaceutical acceptable formulations, such as granules, oral liquid, capsules, tablets, injections or powder. The Brassica rapa polysaccharide obtained by the method has available raw materials, simple method, low production cost, high polysaccharide content in the product, stable quality and easy control. The Brassica rapa polysaccharide has obvious anti-hypoxia activity. The Brassica rapa polysaccharide can be used for preparing the drugs or health care foods for prevention or/and treatment of low oxygen, hypoxia, hemic hypoxia, circulatory hypoxia and toxic hypoxia.

The invention relates to a novel method for extracting a seaweed intermediate by an enzyme process, which comprises the following steps: sufficiently cleaning and immersing dry Laminaria digitata, adding into an acidic solution, and stirring uniformly to obtain a mixture A; dissolving a composite acidic enzyme in water, and adding into the mixture A to perform primary stirring extraction, thereby obtaining a mixture B; dissolving an inorganic alkaline compound and a composite alkaline enzyme in water, and adding into the mixture B to carry out secondary stirring extraction, thereby obtaining the mixture C; and dissolving a preserving agent in water, adding into the mixture C, and stirring uniformly to obtain the seaweed intermediate. The method for preparing the seaweed intermediate is efficient, quick, mild, comprehensive, safe and harmless. Compared with the traditional acid-alkali extraction technique, the extraction efficiency is enhanced by 50% above, the algal polysaccharides content is enhanced by 50-100%, the alginic acid content is 3.5-4.0, the effect of the seaweed intermediate product is obviously enhanced, and all the effective components in the seaweeds are reserved in the product, thereby implementing complete-effect addition in deed.

The invention discloses a method for planting dendrobium officinale on rocky desertification land. The method comprises the steps of selecting planting environments on the rocky desertification land, and respectively planting the dendrobium officinale according to the planting environments. 1, for the planting environment of a rocky mountain with live mosses grown originally, the dendrobium officinale seedlings are directly planted in the mosses; 2, for the planting environment of a rocky mountain without mosses originally, the roots of the dendrobium officinale are wrapped with mosses, and the roots of the dendrobium officinale are adhered to rock walls together with the mosses by water-retaining adhesive; 3, for the planting environment of a low-lying area without ponding, a culture substrate which is 8-12cm thick is directly paved, and the dendrobium officinale seedlings are planted into the culture substrate; 4, for the planting environment of a low-lying area easy to water, the low-lying area is filled up or filled to have a depth of 25-35cm by stones as a drainage layer, then a culture substrate is paved, and the dendrobium officinale seedlings are planted into the culture substrate. According to the method, the rocky desertification land resources are fully used, the ecological environment is improved, and the grown dendrobium officinale is high in yield and good in quality.

The invention discloses a method for cultivating triploid dendrobium huoshanense by a crossbreeding method of tetraploid plants and diploid plants. The method comprises the following steps: (1) dendrobium huoshanense species which are from different producing areas and have characteristics of good stress resistance, rapid growth, thick stalk, long stem, many tillers, low fiber content and high active ingredient content are preferably selected as polyploidy cultivated original plant parents; (2) seeds of the different species of original plants undergo aseptic sowing in a solid medium so as to obtain diploid protocorms; (3) protoplasts of the different species of diploid protocorms are prepared and cell fusion is carried out; (4) detected tetraploid plants undergo asexual tissue culture so as to obtain tetraploid plant seedlings; (5) the tetraploid plant seedlings undergo hardening-seedling acclimatization and cultivation to flowering; (6) the tetraploid plants and diploid plants undergo artificial pollination and crossbreeding, and fructification is completed to obtain triploid dendrobium huoshanense seeds; and (7) the triploid seeds undergo aseptic sowing and culture so as to obtain a lot of triploid seedlings.

Belonging to the technical field of Dendrobium officinale planting, the invention discloses a Dendrobium officinale tissue culture seedling medium, which is composed of the following components: apple puree, banana puree, white sugar, ferrous sulfate, ammonium nitrate, potassium nitrate, magnesium sulfate, potassium dihydrogen phosphate, boric acid, glycine, vitamin B1, vitamin B2, sugar amino acid, naphthyl acetic acid, and tap water. The Dendrobium officinale tissue culture seedling medium has the characteristics of reasonable collocation and comprehensive nutrition, can quickly promote rooting and stem growth, increases the Dendrobium officinale polysaccharide content and amino acid content, shortens the culture cycle of Dendrobium officinale, and improves the Dendrobium officinale transplanting survival rate.