2results about How to "High in polysaccharides" patented technology

This invention belongs to the field of plant tissue culture technology, specifically disclosing a method for producing Polygonatum polysaccharides using Polygonatum yunnanense tissue culture, comprising the following steps: explant disinfection, callus induction, callus proliferation and induction culture: selecting vigorous callus tissue, transferring it to a culture medium: White medium + 2,4-D 1.5 mg / L + VC 20 mg / L + sucrose 40 g / L + 30 mg / L inducer + agar 6 g / L, pH 5.8, after culture, collecting the callus, drying it, and then extracting Polygonatum yunnanense polysaccharides; the inducer is Polygonatum yunnanense endophytic basidiomycetes (… Talaromyces sp. The fermentation broth extract of HJF23; the HJF23 preservation number is CCTCC NO: M2021344. This invention not only realizes the efficient biosynthesis of Polygonatum yunnanense polysaccharide, but also significantly enhances its functional activity.

The present application relates to the technical field of traditional Chinese medicine processing, and particularly relates to a processing method for regulating the quality of Yunnan rhizoma polygonati by stress, which uses fresh Yunnan rhizoma polygonati as raw material, and adopts a programmed temperature control method to perform stress, washes, processes, slices and dries the stressed Yunnan rhizoma polygonati to obtain a finished product; the stress is heat stress or cold stress. Compared with the prior art, the present application gradually changes the stress of the collected fresh Yunnan rhizoma polygonati, avoids the damage to the physiological structure of the plant caused by the rapid change or large amplitude of the temperature, and thus affects the ability and efficiency of the plant to secrete secondary metabolites. The present application significantly increases the polysaccharide content of Yunnan rhizoma polygonati from the source production site processing link, improves the activity of sucrose synthase, and improves the quality of Yunnan rhizoma polygonati. The present application is simple and easy to operate, and provides a new idea for the new production site processing method of medicinal Yunnan rhizoma polygonati.