Extracting method for active polysaccharide in higher plant or edible and medicinal fungi

A technology for edible and medicinal fungi and higher plants, which is applied in the field of high-efficiency extraction of active polysaccharides in plants or edible and medicinal fungi, can solve the problem of low extraction rate, achieve simple solvent recovery, increase polysaccharide content, and low average molecular weight Effect

Active Publication Date: 2012-04-18
张劲松
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Simple solvent immersion can only dissolve the free polysaccharides in the cell, but can't do anything to the polysaccharides in the cell wall, so the extraction rate is generally not high

Method used

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  • Extracting method for active polysaccharide in higher plant or edible and medicinal fungi
  • Extracting method for active polysaccharide in higher plant or edible and medicinal fungi

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] like figure 1 As shown in the schematic diagram of the technological process, the specific process is as follows:

[0035] 1) Take 1.5 kg of dried, impurity-removed, and mechanically crushed Astragalus membranaceus in a Chinese herbal medicine extraction container, add 10 L of ethanol, heat and reflux for extraction for 1 hour, and repeat the above process once;

[0036] 2) Filtrate, combine the two filtrates and recover ethanol by distillation under reduced pressure to obtain an alcoholic extract rich in fat-soluble active ingredients such as flavonoids, saponins, and coumarin, and partially dry the filter residue for subsequent use;

[0037] 3) taking the dried filter residue in step 2) and placing it in the extraction container;

[0038] 4) Take 180g of oxalic acid and add 2.0L of water to make an oxalic acid solution;

[0039] 5) Take 2L of the oxalic acid solution in the above step 4), add it to the extraction container in the step 3), stir fully to make the filt...

Embodiment 2

[0048] The difference from Example 1 is:

[0049] 1) 1.5kg papaya is placed in the extraction container after drying, removing impurities, and mechanically pulverizing;

[0050] 2) Take 0.8L of anhydrous acetic acid, add 0.15L of water, add 0.05L of dilute hydrochloric acid with a mass percentage concentration of 5%, and make 1L of acetic acid\hydrochloric acid mixed acid solution;

[0051] 3) Take 1L of the mixed acid solution in step 2), add it to the extraction container in step 1), stir well to make it evenly wet to obtain the mixed material;

[0052] 4) Heat the mixed material in step 3) to 80° C., keep it warm for 60 minutes, and carry out vacuum distillation until there is no liquid in the extraction container.

[0053] 5) Add 5L of 95% ethanol solution in the extraction container in step 4), fully stir, filter, and distill the filtrate to recover ethanol to obtain the alcoholic extract rich in papaya flavonoids. After the filter residue is partially dried, it is organ...

Embodiment 3

[0060] The difference from Example 1 is:

[0061] 1) 1.5 kg of dried, impurity-removed, and pulverized Angelica sinensis was placed in an extraction container, 7 L of petroleum ether was added, heated and refluxed for extraction for 1 hour, and the above process was repeated once;

[0062] 2) Filtrate, combine the two filtrates and distill under reduced pressure to recover petroleum ether to obtain the volatile oil of Angelica sinensis, and dry the filter residue of Angelica sinensis for later use;

[0063] 3) Part of the dried angelica filter residue in step 2) is placed in an extraction container, 10L of ethanol is added, heated to reflux for extraction for 1 hour, and the above process is repeated once;

[0064] 4) Filtrate, combine two filtrates and recover ethanol by vacuum distillation to obtain an alcoholic extract rich in angelica flavonoids, and partly dry the filter residue for later use;

[0065] 5) get the Angelica sinensis filter residue that dries in step 4) and...

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Abstract

The invention relates to an extracting method for an active polysaccharide in a higher plant or edible and medicinal fungi. The extracting method comprises the following steps: adding an organic acid or an organic or inorganic mixed acid solution in raw materials; heating the raw materials, utilizing the molecules of the organic acid to separate the active polysaccharide from the raw materials, and moderately cutting off glucosidic bonds of macromolecular active polysaccharide of the plant, thereby realizing local degradation; adopting a distilling, filtering or centrifuging method to remove most acid solution in the raw materials, and then washing with an organic solvent, thereby removing few remained acid solutions; extracting by adding water with the volume being 5-15 times that of theraw materials into the raw materials processed with the organic acid, and after concentrating an extracting solution, settling alcohol, thereby obtaining rough active polysaccharide; dissolving the rough active polysaccharide in de-ionized water with the volume being 5-15 times that of the rough active polysaccharide; adjusting pH of the solution to be neutral; and centrifuging, dialyzing or film-separating liquid supernatant, and then concentrating and drying, thereby obtaining an active polysaccharide product with low average molecular weight, narrow molecular weight distribution of the polysaccharide and excellent water solubility. Various defects, such as water consumption and energy consumption caused by technology, low yield of products, and the like, are overcome.

Description

technical field [0001] The invention relates to the field of extraction of active ingredients of natural products, in particular to a high-efficiency extraction method of active polysaccharides in higher plants or edible and medicinal fungi. Background technique [0002] Polysaccharides are natural macromolecular compounds formed by combining multiple monosaccharide molecules with glycosidic bonds, and are one of the basic substances that constitute life. In the past two decades, with the development of molecular biology and cell biology, it has been found that polysaccharides have a variety of biological functions, and polysaccharides and their conjugates participate in various life activities in cells, such as cell-specific recognition, composition Receptors of various antigens and drugs on the cell surface, activation of immune cells, etc. Therefore, polysaccharide research has aroused great interest. Polysaccharides can be generally divided into fungal polysaccharides, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 张劲松李明天许磊刘志宇
Owner 张劲松
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