The invention relates to a hill potherb cultivation method, particularly to a method for high-yield cultivation of pteridium aquilinum var.latiusculum on mountain, and belongs to the technical field of rapid plant breeding. The technical scheme of the invention mainly comprises: growing bud by using spore as a raw material, washing and disinfecting explants, culturing clustered shoots, carrying out stem separating culture on the clustered shoots, acclimatizing tissue culture seedling, transplanting, and spraying a selenium-rich nutrition solution. According to the present invention, the problems that the seedling source is short, the seedling exercising survival rate and the transplanting survival rate are low, the yield is not high, and the like during the large-area artificial cultivation of the pteridium aquilinum var.latiusculum are saved; and with the high-yield cultivation method, the seedling propagation rate is increased by hundreds of times, the seedling exercising survival rate and the transplanting survival rate are increased to more than 97%, the yield is increased to more than 34%, the powerful assurance is provided for the promotion of the large-scale development of the pteridium aquilinum var.latiusculum industry as soon as possible, and the good social and economic benefits are provided.