90results about How to "Promote maturation" patented technology

The invention provides cell-adherent polymeric coatings for articles having a nanofibrillar structure. The coatings include a synthetic, non-biodegradable polymer having at least one pendent amine group, wherein the polymer is covalently immobilized on the article via latent reactive groups. The invention also provides methods for the long term culturing of cells using the polymer coated nanofibrillar structures. The polymer coated nanofibrillar structures of the invention have been found to be particularly useful for the growth and differentiation of cells, including neural precursors.

The invention relates to a micro-ecological preparation and the application thereof, and specifically relates to a micro-ecological preparation containing bacillus coagulans as a main active ingredient and application of the preparation in prevention or treatment of relating diseases.

The invention discloses a medicinal composition, which comprises Chinese medicinal materials 30-99.9% of the total weight of the composition, and pharmaceutically acceptable carries in balancing amount. The Chinese medicinal materials include the following Chinese traditional medicines by weight: (a) 15-90 parts of Chinese medicinal materials for providing sperms motion energy, (b) 15-60 parts of Chinese medicinal materials for strengthening adenosine triphosphate, (c) 70-330 parts of Chinese medicinal materials for providing antioxidation effect, (d) 10-50 parts of Chinese medicinal materials for promoting sperms generation, (e) 10-40 parts of Chinese medicinal materials for regulating internal secretion, and (f) 20-80 parts of Chinese medicinal materials for invigorating qi and strengthening spleen. The invention also discloses a preparation method and an application thereof.

The invention relates to an infant formula comprising a source of protein in an amount of not more than 2.0 g / 100 kcal, a source of lipids, a source of carbohydrate and a probiotic wherein the probiotic is present in an amount equivalent to between 102 and 105 cfu / g of dry formula. The invention further extends to the use of such an infant formula to modulate the immune system of a neonatal infant to promote the development in the first few weeks of the life of the infant of a beneficial intestinal microbiota comparable with that found in breast fed babies as well as to promote the maturation of the immune system of a neonatal infant in the first few weeks of life.

The invention relates to a processing method allowing the seeds of Paris polyphylla var. yunnanensis to grow roots in four months and to grow leaves in eight months. The method comprises the following steps: step 1, in early September, selecting mature seed capsules of Paris polyphylla var. yunnanensis, spreading the seed capsules to low density and drying the seed capsules in the shade for 30 days; step 2, in early October, standing the Paris polyphylla var. yunnanensis seeds dried in the shade at a low temperature for 15 to 20 days to realize low temperature treatment; step 3, in mid-October, stripping capsule hulls of the Paris polyphylla var. yunnanensis seeds having undergone low temperature treatment, sowing the Paris polyphylla var. yunnanensis seeds with red coats in prepared seedling trays and carrying out seed germination; step 4, putting the seedling trays treated in the third step in a cold storage and carrying out low temperature treatment for 15 to 20 days; step 5, in mid-January, sowing the Paris polyphylla var. yunnanensis seeds having undergone low temperature treatment in the fourth step in a field with an altitude of 2800 meters; and step 6, carrying out manual weeding. In mid-April, a part of small seedlings of Paris polyphylla var. yunnanensis grow leaves; and in mid-June, more than 85% of the small seedlings of Paris polyphylla var. yunnanensis grow leaves. With the method of the invention, the germination rate and the seedling rate of the Paris polyphylla var. yunnanensis seeds reach more than 85%.

The invention relates to a preparation for enhancing human immunity and promoting growth and development. The preparation is prepared from the following raw materials in parts by mass: 200-300 parts of bovine colostrums, 50-75 parts of mushroom extract, 5-15 parts of zinc lactate, 7.5-15 parts of vitamin C and 800-1000 parts of isomaltose oligosaccharide and a proper amount of pharmaceutic adjuvant (such as magnesium stearate and the like) through the processes of as mixing, granulating, tabletting or bagging and the like so as to prepare granules, tablets and the like. The preparation provided by the invention has the beneficial effects that: the immune globulin contained in the preparation can be combined with antigens such as pathogenic microorganism, toxin and the like to form an antibody, the development and the maturation of the autoimmune system of newborn mammal cubs are promoted in the mean time and the cubs are protected from pathogen invasion; lentinan, zinc and the vitaminC are simultaneously combined to ensure that the effect of enhancing immunity is more remarkable; meanwhile, taurine, choline, phospholipid, brain peptide and the like which are contained in the bovine colostrums have the effects of promoting the growth and the development of children and promoting the intelligence development.

The invention belongs to the field of animal husbandry, and particularly relates to a method for promoting female and male parent giant salamanders to synchronously mature. The method is suitable for artificial rearing of giant salamanders. The method is characterized by being realized through the following mode: firstly, selecting sexually-mature parent giant salamanders, and artificially domesticating the parent giant salamanders to enable the same to be capable of taking in food initiatively; and secondly, feeding spawning-inducing medicine to the artificially-domesticated parent giant salamanders from the middle of August to the beginning of September in each year, wherein the spawning-inducing medicine is prepared by mixing luteinizing hormone releasing hormone analogs (LHR-A), human chorionic gonadotrophin (HCG) for fishes and carp pituitary glands. The method of feeding the spawning-inducing medicine is adopted to promote sexual glands of the parent giant salamanders to mature, so that sperm producing amount of male parent giant salamanders is increased by at least 35%, sperm activity is improved by more than 40%, egg laying amount of female parent giant salamanders is increased, and success rate of artificial fertilization is increased by more than 85%.

The invention relates to a livestock synchronous ovulation and fixed-time artificial insemination method. The method includes: intramuscularly injecting GnRH (gonadotropin releasing hormone) or analogues thereof to cows in any day of an estrus period, injecting PG (prostaglandin) or analogues thereof after 7-8 days, and injecting the GnRH or the analogues thereof for the second time after 2-3 days. Twice fixed-time artificial insemination in advance or twice fixed-time artificial insemination in delay is carried out respectively according to follicular development conditions of the cows. By regulation of follicular development time of the cows, fixed-time artificial insemination is carried out in relatively fixed estrus time to increase sperm-egg binding probability and the estrus conception rate of the cows, and accordingly reproduction efficiency of the cows is improved. In addition, compared with a conventional OFAI (ovsynch and fixed-time artificial insemination) technology, the livestock synchronous ovulation and fixed-time artificial insemination method has the advantage that the estrus conception rate is increased by 10%-15% due to avoiding of estrus diagnosis before insemination, thereby having promising market application prospect.

The invention discloses a water culturing and transplanting method for apple tissue culture seedlings. The method includes the steps of greenhouse exercising of rooting container seedlings, cleaning of the tissue culture seedlings, preparing of a water culturing facility, water culturing of the rooting seedlings and outdoor transplanting management. According to the water culturing and transplanting method for the apple tissue culture seedlings, due to the physiological feature that before the apple tissue culture seedlings are taken out of a container, complete cuticles are not formed on blades of the apple tissue culture seedlings and the effective photosynthesis cannot be conducted, nutrient solutions are used for water culturing, under an appropriate environment, the epidermal cells of the blades of the tissue culture seedlings are promoted to develop to be mature, transformation from heterotrophic metabolism to autotrophic metabolism is fast completed, and the purpose of improving the transplantation survival rate of the apple tissue culture seedlings is achieved. The technology is easy to implement and low in cost, and the transplantation survival rate can be 90 percent or more.

A slow-releasing oxytocic for artificially inducing the maturation of epinephelus' sex gland and the ovulation or ejaculation by embedding it in body of epinephelus is prepared from luliberin like, 17 alpha-hydroxyprogesterone, cholesterin, cellulose and plant oil through proportional mixing and moulding.

The invention discloses a mmethod for obtaining analogous retinal tissues rich in cone and rod cells by using human induced pluripotent stem cells. The method comprises the following steps: digestinghiPSCs to obtain cell pellets, and then carrying out suspension cultivation on the cell pellets to obtain an embryoid body; reinoculating the embryoid body into a culture dish enveloped by Matrigel, and carrying out induced differentiation inside an induced cultivation solution to obtain nerve retina (NR) and RPE; stirring up the NR and the RPE, carrying out suspension cultivation to obtain 3D analogous retina comprising NR tissues, then carrying out continuous suspension cultivation inside an optimized culture solution without retinoic acid, carrying out NR differentiation on all retinal cells, comprising highly matured photoreceptor cells, Rhodopsin positive rod cells, L / M OPSIN positive red and green cone cells and S-OPSIN positive blue cone cells, and thus particularly obtaining the analogous retinal tissues rich in red and green cone and rod cells.

The invention provides an epinephelus septemfasciatus parent fish reproduction and maturing bait and a preparation method thereof. The epinephelus septemfasciatus parent fish reproduction and maturing bait is formed by following components in percentage: 30% of artificially-matched powder feed (main components comprise fish meal, starch, soybean meal powder, peanut bran powder and the like), 10% of opossum shrimp powder, 10% of oyster / mya arenaria linnaens meat (fresh and alive products or chilled products), 20% of sardinella zunasi (chilled products), 3.2% of concentrated micro-mud marimo, 10% of loligo japonica (fresh and alive products or chilled products), 10% of clam worms (fresh and alive products or chilled products), 5% of brine shrimp powder, 0.5% of compound vitamin, 0.4% of astragalus membranaceus powder, 0.4% of lecithin powder and 0.5% of immune polysaccharides. The bait disclosed by the invention not only can guarantee the nutritional requirement of parent fish, but also can effectively improve the developmental rate of parent fish sexual glands, accelerate the sexual glands to synchronously develop and improve the egg laying amount and the ovum quality of female parent fish, so as to lay a foundation for large-scale larval rearing of epinephelus septemfasciatus.

A lyophilizate, method of preparation, and use of the lyophilizate for gonad protection is described. The lyophilizate comprises cetrorelix dissolved in 30% (v / v) acetic acid, transferred to water, and freeze-dried.

The invention relates to a Chinese herbal medicine additive for promoting growth of laying hens and improving egg quality and a feeding method thereof. The additive is prepared from the following Chinese herbal medicines in parts by weight of 2-4 parts of radix astragali, 1.5-3.5 parts of poria cocos, 1-3 parts of radix isatidis, 1-3 parts of radix codcnopsitis pilosulas, 1-2.5 parts of radix glycyrrhizae, 2-4 parts of fructus crataegi, 3-5 parts of rhizoma dioscoreae, 3-5 parts of fructus hordei germinatus, 1-2 parts of massa medicata fermentata, 1-2 parts of agastache rugosus, 1-2 parts of pericarpium citri reticulatae and 1-2.5 parts of herba ephedrae. Various Chinese herbal medicines are ground and sieved, and the sieved materials are mixed to obtain a mixture, namely the Chinese herbal medicine additive. 2 kg of the additive is added into every 100 kg of chicken compound feed according to the weight ratio to obtain chicken feed. 10-36 g of the chicken feed is used for feeding eachbrooding chicken every day, 40-80 g of the chicken feed is used for feeding each growing chicken every day, and 85-120 g of the chicken feed is used for feeding each laying hen every day. The additive has the characteristics of strong safety, small toxic and side effects and low residue, avoids tolerance of livestock and poultry to the additive, and basically realizes antibiotic-free breeding.

The invention belongs to the field of foods, and particularly discloses hypoallergenic infant formula goat milk powder. The hypoallergenic infant formula goat milk powder is prepared from raw goat milk, high-oil desalted goat whey powder, lactose, goat whey protein powder, soybean oil, 1, 3-dioleic acid-2-palmitoyl triglyceride, linseed oil, anhydrous butter, arachidonic acid powder, fish oil powder, composite mineral substances, decavitamin, lactoferrin, calcium carbonate, osteopontin, potassium hydroxide, choline chloride, nucleotide, phospholipid and lactobacillus paracasei N1115. The composition of the hypoallergenic infant formula goat milk powder is close to breast milk, nutrition for growth and development of infants is guaranteed, milk protein allergy of newborn infants is avoided,immunity of the newborn infants is enhanced, and therefore the newborn infants are prevented from being infected and getting sick. The hypoallergenic infant formula goat milk powder is suitable for being eaten by infants of 0-6 months old, and is particularly suitable for being eaten by newborn infants with low resistance.

A method for enhancing cognitive development in a pediatric subject involving administering to the subject a nutritional composition which includes up to 7 g / 100 Kcal of a fat or lipid; up to 5 g / 100 Kcal of a protein or protein equivalent source; 0.06 g / 100 Kcal to 1.5 g / 100 Kcal of an enriched milk product; 5 mg / 100 Kcal to 90 mg / 100 Kcal of a source of long chain polyunsaturated fatty acid; and 0.015 g / 100 Kcal to 1.5 g / 100 Kcal of a prebiotic.

The invention relates to a xenocypris davidi bleeker fry expanding propagation method, which comprises the following steps of (1) reserve parent fish selection and culture; (2) parent fish selection and culture; (3) artificial regulation and control on oosperm incubation; and (4) fry culture. The xenocypris davidi bleeker fry expanding propagation method has the advantages that the design is reasonable, the operability is high, in the parent fish and fry culture process, aquatic plants are used as major raw materials, resources are rich, the feeding cost is low, parent fish compound feed is fed in the parent fish culture process, potential diseases in parent fish bodies can be prevented, the immunologic function of the parent fish bodies can be enhanced, the maturation of the parent fish gonad is promoted so that the parent fish realizes the nature spawning, the spawning quantity of the parent fish is increased, the propagation capability is improved, and meanwhile, the quality of spawn particles and seminal fluid is improved; special juvenile fish feed is fed in the juvenile fish stage, the weight of adult fish and the survival rate of the juvenile fish of xenocypris davidi bleeker can be respectively and effectively increased and improved, the feed coefficient is reduced, and the feed conversion rate is improved.

The invention relates to a method for artificially propagating parabramis pekinensis. The method comprises the following steps of: 1) selecting the source of parent fish; 2) breeding the parent fish; 3) artificially spawning and performing insemination, namely a) selecting the parent fish, b) collecting semen, taking eggs and checking, c) matching, d) setting water temperature of spawning, e) injecting odinagogue, and f) laying eggs; and 4) performing hatching management. By the means of enhancement of parent fish nutrient enhancement cultivation, flowing water stimulation regulation and the like, the gonad development and maturation of the parent fish and discharge can be promoted, the artificial spawning and insemination processes are standardized, the technical problem in the artificial propagation of parabramis pekinensis is effectively solved, the spawning rate of artificial propagation is 89 percent, the fertility rate is 83 percent, and the hatching rate is 83.5 percent. The method is easy and convenient to operate and high in applicability and can meet the requirement on the industrial batch propagation.

A novel lyophilizate and method of preparation as well as the use of the lyophilizate to treat female infertility and for gonad protection. Cetrorelix is dissolved in acetic acid 30% v / v, the solution is transferred to water and freeze dried.

A method for enhancing cognitive development in a pediatric subject involving administering to the subject a nutritional composition which includes up to 7 g / 100 Kcal of a fat or lipid; up to 5 g / 100 Kcal of a protein or protein equivalent source; 0.25 g / 100 Kcal to 16 g / 100 Kcal of buttermilk; 5 mg / 100 Kcal to 90 mg / 100 Kcal of a source of long chain polyunsaturated fatty acid; and 0.015 g / 100 Kcal to 1.5 g / 100 Kcal of a prebiotic.

The invention discloses a complete feed and a premix for promoting body type and skeletal development of laying chicks and a preparation method of the complete feed and the premix. The premix comprises mineral elements, compound vitamins, compound amino acids, functional substances, protein raw materials, energy raw materials and carriers, and aims to solve the technical problems of poor body typeuniformity and skeletal development inhibition due to unbalanced nutrition of the laying chicks. Compared with commercially available feeds, the premix and the complete feed for the chicks prepared by the method can remarkably improve the feed intake and the weight of the chicks, reduce the death and culling rate, effectively improve the uniformity of chicken flocks, and increase the shin lengthof the chicks.

The invention relates to electrolysis multi-vitamins for sows and a preparation method thereof. The electrolysis multi-vitamins for sows are prepared from, by weight, 10-30 parts of sow composite multi-vitamins, 30-50 parts of glucosum anhydricum, 1-10 parts of potassium chloride, 1-10 parts of sodium chloride, 1-3 parts of magnesium chloride, 0.1-2 parts of L-carnitine, 0.1-1 part of organic chromium with the mass content being 98.5%, 5-10 parts of a radix bupleuri extract, 2-8 parts of a poria cocos extract, 5-15 parts of fructo-oligosaccharide and 20-40 parts of anhydrous sodium sulphate. Due to the compatibility of the medicines, the electrolysis multi-vitamins have the advantages of clearing away heat, removing dampness, promoting reproductive organ to be matured, enhancing the phagocytic function of macrophages, enhancing the body disease resistance and anti-stress capacity, increasing the ovulation number and the embryo survival rate, increasing the sow litter rate, and meanwhile preventing calcium deficiency, sand crack and other diseases.

The invention relates to a cutting seedling-raising method for a poplar tree. The cutting seedling-raising method comprises the following concrete steps: (1) control of cutting conditions; (2) pretreatment of cuttings; (3) secondary pretreatment of the cuttings; and (4) daily management with attention to a humidity which should not exceed 60% in a greenhouse, so propagation of residual bacteria on the surface of leaves is reduced. Experimental results show that the cutting seedling-raising method for the poplar tree provided by the invention raises a poplar tree seedling with stronger disease resistance; a conventional method raises the poplar tree seedling with a disease incidence rate up to 30.4% in 6 months, needs to spray a disease prevention agent, and does not facilitate growth and development of the seedling in an early stage; meanwhile, the method provided by the invention raises the seedling with the disease incidence rate to be 9.5% or below, greatly reduces the use of chemicals, facilitates growth and development of the seedling, and raises the seedling with higher quality.

The invention discloses a traditional Chinese medicine composition for antibiotic-free breeding of free-range chickens and belongs to the technical field of free-range chicken breeding medication. The traditional Chinese medicine composition is mainly prepared from, by weight, 2.5-10 parts of radix astragali, 2-8 parts of lilium brownii, 2-8 parts of radix ophiopogonis, 2-8 parts of herba houttuyniae, 1.5-6 parts of radix paeoniae alba, 1.5-6 parts of radix platycodonis, 1.5-6 parts of radix codonopsis, 1-4 parts of radix bupleuri, 1-4 parts of fritillary bulb, 1-4 parts of radix angelica sinensis and 1-4 parts of radix glycyrrhizae. The traditional Chinese medicine composition for antibiotic-free breeding of the free-range chickens is used for preventing chicken diseases by substituting a Chinese herbal medicine preparation for antibiotics and is simple and convenient to use and good in use effect.

The invention relates to a multi-vitamin crop nutrient cold resistant agent and a preparation process thereof. In recent years, the problems of water loss, soil erosion, quality reduction, food pollution, ecological imbalance and the like are very serious because of wide application of fertilizer. The multi-vitamin crop nutrient cold resistant agent comprises the following components according to the weight portion: 1-100ppm of vitamin B5, 1-100ppm of vitamin B6, 1-100ppm of vitamin B12, and the balance of de-ionized water or distilled water. The invention is used for agricultural output increment and cold resistance.

The invention discloses a preparation method of tumor immune antigen, and products and application of the preparation method. Concretely, the inventor finds that after local sequential cooling-heating treatment (or cold-heat treatment) is carried out on tumor tissues and / or cells, the tumor tissues and / or cells release a great deal of tumor immune antigen such as heat shock protein 70 both in vivo and in vitro, and the release amount is significantly higher than the amount released by the tumor tissues and / or cells which are only subjected to pure thermal stimulus. After the sequential cooling-heating treatment is carried out, the obtained tumor immune antigen is capable of effectively activating the tumor immune system in a body, such as immunosuppressive cells can be effectively converted into mature dendritic cells, so that the presentation of the immune antigen is improved and tumor immunity is activated.

The invention relates to the technical field of scalp care, in particular to hair-strengthening essence and a using method thereof. The hair-strengthening essence is prepared from the following components in parts by weight: 0.1 to 5 parts of a nasturtium officinale extract, 0.1 to 2 parts of MetaTrix APL 3P powder, 0.01 to 1 part of a rhizoma chuanxiong extract, 0.01 to 1 part of a radix sophorae flavescentis extract, 0.01 to 1.0 part of a ligustrum lucidum fruit extract, 0.01 to 1 part of a tree bark extract and 0.01 to 1 part of pyridoxine HCL. The traditional Chinese medicine composition has the advantages of good hair growth effect, difficult recurrence and no side effect.

The invention discloses a liquid seaweed bio-fungus and a preparation process thereof. The liquid seaweed bio-fungus is compounded by the marine bio-fungus and three microbial floras including actinomycetes, lactobacillus and saccharomycetes. The process comprises the following steps: sterilizing the seaweeds at high temperature (over 100 DEG C), soaking, grinding and rubbing the seaweeds to prepare seaweed starch; proportionally mixing the seaweed starch with molasses, various auxiliaries, water and cultured fungus solution, stirring and then fermenting; and finally packaging. The preparation process of the invention is simple and is characterized by low production cost, high concentration and high survival rate. The liquid seaweed bio-fungus prepared by the process has the characteristics of high activity, quick absorption, high economic benefits and good social and environmental benefits.

The invention provides cell-adherent polymeric coatings for articles, the coating including a synthetic, non-biodegradable polymer having a plurality of pendent amine groups, wherein the polymer is covalently immobilized on the article via latent reactive groups. The invention also provides methods for the long-term attachment of cells using the polymeric coatings.