Water culturing and transplanting method for apple tissue culture seedlings

A tissue culture seedling and hydroponic technology, applied in the field of plant tissue culture, can solve problems such as poor transplanting effect, and achieve the effects of low cost, simple implementation, and improved transplanting survival.

Inactive Publication Date: 2014-08-20
天水市果树研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a hydroponic transplanting method for apple tissue cultured see

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Apple tissue culture technology includes the establishment of explants, multiplication culture, rooting culture, transplanting and other links, of which:

[0018] Establishment of explants: Cut the top buds or side buds of annual young apple trees that grow vigorously and finely in the field, rinse with tap water, disinfect the surface with 74% alcohol, sterilize with 0.1% mercury liter, and finally rinse with sterile water Finally, the induction medium is connected to the induction medium on the ultra-clean workbench of the sterile room, and the induction differentiation is carried out in the culture room, and the non-toxic shoot tip is obtained as an explant for culture;

[0019] Expansion and cultivation: Cut the non-toxic shoot tips obtained by the above method into 2cm stem segments, inoculate them into the expansion medium on the ultra-clean workbench in the sterile room, pick 4 buds from each bottle of medium, and put them in the cultivation room. Proliferation; ...

Embodiment 2

[0030] Apple tissue culture technology includes the establishment of explants, multiplication culture, rooting culture, transplanting and other links, of which:

[0031] Establishment of explants: Cut the top buds or side buds of annual young apple trees that grow vigorously and finely in the field, rinse with tap water, disinfect the surface with 74% alcohol, sterilize with 0.1% mercury liter, and finally rinse with sterile water Finally, the induction medium is connected to the induction medium on the ultra-clean workbench of the sterile room, and the induction differentiation is carried out in the culture room, and the non-toxic shoot tip is obtained as an explant for culture;

[0032] Expansion and cultivation: Cut the non-toxic shoot tips obtained by the above method into 2cm stem segments, inoculate them into the expansion medium on the ultra-clean workbench in the sterile room, pick 3 buds from each bottle of medium, and place them in the cultivation room Proliferation;...

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PUM

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Abstract

The invention discloses a water culturing and transplanting method for apple tissue culture seedlings. The method includes the steps of greenhouse exercising of rooting container seedlings, cleaning of the tissue culture seedlings, preparing of a water culturing facility, water culturing of the rooting seedlings and outdoor transplanting management. According to the water culturing and transplanting method for the apple tissue culture seedlings, due to the physiological feature that before the apple tissue culture seedlings are taken out of a container, complete cuticles are not formed on blades of the apple tissue culture seedlings and the effective photosynthesis cannot be conducted, nutrient solutions are used for water culturing, under an appropriate environment, the epidermal cells of the blades of the tissue culture seedlings are promoted to develop to be mature, transformation from heterotrophic metabolism to autotrophic metabolism is fast completed, and the purpose of improving the transplantation survival rate of the apple tissue culture seedlings is achieved. The technology is easy to implement and low in cost, and the transplantation survival rate can be 90 percent or more.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for hydroponic transplanting of apple tissue cultured rooted seedlings. The transplanting method can make the transplanting survival rate more than 90%, and has simple management and low production cost. Background technique [0002] In recent years, with the rapid development of science and technology, plant tissue culture technology has entered the stage of production application, widely used in large-scale in vitro rapid propagation of flowers and fruit trees, and test-tube seedlings have formed industrialization in the international market. Tissue culture is an important means of detoxification of apples and realizes the rapid breeding of seedlings. The cultivation application of tissue culture detoxification seedlings instead of ordinary seedlings has become more and more extensive, and the application prospect is also very broad. [0003] Plant tissue ...

Claims

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Application Information

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IPC IPC(8): A01G31/00C05G1/00
Inventor 李帼英赵新红王花吴晔杨映红杨俊霞杨瑞斌周代琴马建芳王艳芳李海青
Owner 天水市果树研究所
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