The invention belongs to the field of aquatic feeds, and specifically relates to a feed for tendering the meat quality of grass carp and a preparation method of the feed. The feed comprises broad bean, fish meal, wheat middling, rice bran, rapeseed dregs, glycine betaine, choline chloride, monocalcium phosphate, enzymic preparation, decavitamin and complex mineral salt; and the raw materials are mixed and granulated. According to the feed for tendering the meat quality of grass carp, the problems and defects in the existing grass carp tendering technology can be solved, and a meat quality tendering feed which is balanced in nutrition, convenient to use, short in culture cycle, high in efficiency and environmental-friendly is provided. When the product is used for culturing grass carp, the feed efficiency is improved, the nitrogen and phosphorus discharge in the culture process is decreased, the water quality pollution is lightened, the grass carp growth performance is improved, the disease resisting and anti-stress capabilities are enhanced, and the meat quality of the grass carp is tight, elastic, crisp, tasty and delicious.