Compound microbial preparation for aquaculture and method

A technology of compound microorganisms and aquaculture, applied in the field of compound microorganism preparations for aquaculture, can solve the problems of loss of manpower and material resources, low survival rate, low biological immunity, etc., and achieve the effect of increasing production, reducing disease rate and mortality

Active Publication Date: 2016-02-24
珠海鱼来鱼旺生物科技有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The common problems in the aquaculture industry are: the alkalinity of the aquaculture water is too high or too strong, the content of ammonia nitrogen and nitrite is high; due to the existence of the aforementioned harmful substances in the water body, there are often algal blooms in the aquaculture ponds. , resulting in excessive water color, blackening, redness, turbidity and other aging phenomena, which in turn promotes the breeding of a variety of toxic pathogenic bacteria in the water body, such as Aeromonas hydrophila, Vibrio, Escherichia coli, Streptococcus, etc.; thus causing breeding animals For example, red legs, blind eyes, and rotten limbs appear in shrimp; black gills, swollen gills, rotten gills, blind eyes, and broken whiskers appear in sea fish and freshwater fish; and other sea cucumbers, shellfish, etc. The survival rate is low; and then lead to low biological immunity, disease and fatality rate higher than 20%, seriously affecting the output of aquaculture, increasing the risk of the aquaculture industry, causing a large loss of manpower and material resources; therefore, there is an urgent need for an aquaculture Compound microbial preparation and method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0028] The steps of the above-mentioned compound microbial preparation preparation method are as follows:

[0029] Fermentation: Mix the strains of photosynthetic bacteria, Bacillus subtilis, lactic acid bacteria, nitrifying bacteria, Bdellovibrio and yeast with water in a mass ratio of 1:10, and carry out single-strain sealed fermentation in the culture medium to obtain the fermentation liquid; the culture medium The pH is controlled at 5-7, the culture temperature is controlled at 25-35°C, and the culture time is subject to a pH of 3-6 at the end of fermentation;

[0030] The medium used for fermentation is composed of 15% amino acid, 75% peptone, 8% inorganic salt and 2% trace elements by mass ratio; the inorganic salt is composed of 6% sodium chloride, 1.8% hydrogen phosphate Dipotassium and 0.2% potassium dihydrogen phosphate; trace elements are composed of 0.8% calcium, 0.6% iron and 0.6% zinc in mass ratio;

[0031] Bacteria powder preparation: Centrifuge and concentra...

Embodiment 1

[0043] Embodiment 1: splash in the pond of cultured shrimp (the water in the pond of choosing is 100m 3 )

[0044] The components of the spilled composite microbial preparation are as follows according to the mass ratio:

[0045] Photosynthetic bacteria powder 0.8%, Bacillus subtilis powder 1%, Lactic acid bacteria powder 1%

[0046] Nitrifying bacteria powder 1%, Bdellovibrio powder 1.5%, Yeast powder 0.5%

[0047] Multivitamin 1.6%, the balance is carrier;

[0048] Among them, the carrier is corncob; the multivitamin is composed of 0.2% vitamin A, 0.3% vitamin B1, 0.3% vitamin B2, 0.3% vitamin B6, 0.3% vitamin B12, and 0.2% vitamin C;

[0049] Composite microbial preparations contain 5 billion viable bacteria / g, moisture ≤ 2%;

[0050] Fermentation: the strains of photosynthetic bacteria, Bacillus subtilis, lactic acid bacteria, Bdellovibrio, nitrifying bacteria and saccharomyces were respectively mixed with water at a mass ratio of 1:10 to carry out single-strain sealed...

Embodiment 2

[0056] Embodiment 2: splash in the pond of culture crab (the water in the pond of choosing is 150m 3 )

[0057] The components of the spilled composite microbial preparation are as follows according to the mass ratio:

[0058] Photosynthetic bacteria powder 2%, Bacillus subtilis powder 3%, Lactic acid bacteria powder 1%

[0059] Nitrifying bacteria powder 1.5%, Bdellovibrio powder 4.8%, Yeast powder 1%

[0060] Multivitamin 2.25%, the rest is carrier;

[0061] Wherein, the carrier is corn starch; the multivitamin is composed of 0.3% vitamin A, 0.4% vitamin B1, 0.4% vitamin B2, 0.4% vitamin B6, 0.4% vitamin B12, and 0.35% vitamin C;

[0062] Composite microbial preparations contain 5 billion viable bacteria / g, moisture ≤ 2%;

[0063] The steps of the compound microbial preparation preparation method are as follows:

[0064] Fermentation: the strains of photosynthetic bacteria, Bacillus subtilis, lactic acid bacteria, Bdellovibrio, nitrifying bacteria and saccharomyces were...

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PUM

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Abstract

The invention discloses a compound microbial preparation for aquaculture and a method. The preparation is prepared from, by mass, 0.8%-2% of photosynthetic bacterium powder, 1%-3% of bacillus subtilis powder, 0.5%-1% of lactobacillus powder, 0.5%-1.5% of nitrifying bacterium powder, 1.5%-4.8% of bdellovibrio powder, 0.5%-1% of saccharomycetes powder, 1.6%-2.25% of decavitamin and 84.45%-93.6% of carrier. The preparation is prepared through the steps of fermentation, preparation of bacterium powder and preparation of the preparation. The compound microbial preparation can be used after enlarged cultivation or directly sprayed. When the compound microbial preparation is applied to the aquaculture industry, nitrite, ammonia nitrogen and virosin in water can be decomposed through strain mutualistic symbiosis and enzymolysis, the effects of purifying water and reducing the pH are achieved accordingly, the morbidity of bred creatures can be reduced to a large degree, the income is increased for farmers, and green and healthful aquaculture is achieved.

Description

technical field [0001] The invention relates to a compound microbial preparation and method, in particular to a compound microbial preparation and method for aquaculture. Background technique [0002] Composite microbial preparations are microbial preparations made from two or more beneficial microbial strains that are not antagonistic to each other. Beneficial bacteria use their own physiological metabolism to process organic matter in water, decompose ammonia nitrogen, nitrite, hydrogen sulfide and other adverse factors in water, purify water quality, solve water quality problems caused by various toxins, reduce the risk of breeding animals, and effectively inhibit breeding The occurrence of animal symptoms reduces the need for antibiotics in farmed animals, and the interaction of beneficial bacteria in compound biological preparations is used to achieve healthy farming. In addition, after the action of beneficial bacteria, a large number of good beneficial bacteria group...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34
CPCC02F3/34
Inventor 殷沛玲于海波
Owner 珠海鱼来鱼旺生物科技有限公司
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