171results about How to "Reduce the disease index" patented technology

The invention provides a Chinese medicine residue organic fertilizer. Wastes after extraction of Chinese traditional medicines can be fully utilized, utilization of waste materials is realized, and a bactericidal and insect disease preventing organic fertilizer full of nutritional ingredients is also provided for agricultural production. The Chinese medicine residue organic fertilizer is prepared by fermenting Chinese medicine residue, excrement of animals, solid waste, bacillus subtilis and EM. The Chinese medicine residue organic fertilizer has the advantages that the Chinese medicine residue, excrement of animals and solid waste are mixed, biological fermentation bacteria are added for carrying out rapid fermentation, organic fertilizer is processed, waste materials are changed into things of value, harm is turned into good, the problem of treatment of abandoned herb residue produced by a Chinese medicine factory is solved, and waste treatment cost is also greatly reduced, so that a win-win situation of environmental protection and economic development is realized. By adopting the organic fertilizer made from the Chinese medicine residue, soil can be effectively improved, crop pest also can be effectively prevented, and quality, yield and safety of crops are improved.

The invention discloses streptomyces albidoflavus W68 (with the preservation number CGMCC NO.12210) having wide-spectrum antifungal activity and being separated from deep-sea sludge and application technologies of microbial agents prepared by adopting streptomyces albidoflavus W68 and microbial fertilizers prepared by taking streptomyces albidoflavus W68 as a material. The application technologies of the microbial agents prepared by adopting streptomyces albidoflavus W68 separated from the deep-sea sludge and the microbial fertilizers prepared by taking streptomyces albidoflavus W68 as the material are simple, convenient and efficient, and are environmentally friendly, and the spore concentration of the antifungal preparation obtained through solid fermentation can achieve tens of billions. The pot experiment proves that streptomyces albidoflavus W68 separated from the deep-sea sludge has significant prevention and treatment effects for the soil-borne fungal diseases including wheat root rot, melon fusarium wilt, phytoph-thora capsici leonian and wheat take-all. Streptomyces albidoflavus W68 has great application prospect in the pollution-free prevention and control for the soil-borne fungal diseases.

The invention relates to a method for comprehensively preventing and controlling tobacco bacterial wilt. The method comprises the following steps of: mainly applying a biological anti-microbial agent Qing weisan; supplementing a chemical agent, namely 72 percent agricultural streptomycin sulphate soluble powder; clearing away the refuse in a tobacco field in winter; and taking tillage measures of deep furrow drainage in a field period. The comprehensive preventing and controlling treatment measures have the practical application value of controlling diseases and promoting production and reduce the tobacco loss. Field experiments show that the disease incidence and the disease index of the tobacco bacterial wilt in the comprehensive preventing and controlling field are obviously lower than those of a comparison field, and the relative preventing effect reaches 81.2 percent.

The invention discloses a method for promoting orchid to grow and preventing diseases, and relates to a method for preventing the diseases and promoting orchid branches to grow and prolonging a flowering phase. The method disclosed by the invention comprises the following steps of uniformly mixing a solid state culture and a culture medium into a basin according to the mass ratio of 1 to 50 in orchid plant transplantation, wherein the solid state culture has the spore concentration s 1*10<7>-1*10<8> spore/g and prepared from Trichoderma harzianum T216, and planting an orchid plant, wherein the solid state culture can induce the resistance of the orchid plant to the fungoid diseases such as southern blight, rhizoctonia solani and anthracnose and bacterial soft rot caused by Erwinia carotovara during orchid growth, the plant morbidity is reduced, the disease index is reduced, the orchid is promoted to grow in advance, and the number of blades of the orchid are increased. In addition, the method disclosed by the invention has the advantages that a liquid state culture of the Trichoderma harzianum T216 is regulated into 1*10<5>-1*10<7> spore/mL when the orchid peeps a bud, and the spray treatment is carried out on the orchid plant, so that the branch number of the orchid is increased, and the flowering phase of the orchid is prolonged.

The invention discloses a recombined litopenaeus setiferus protein SF-P9, a preparation method thereof and an application thereof. Besides the invention also relates to coding of an amino acid sequence of the recombined litopenaeus setiferus protein SF-P9 and a DNA sequence corresponding to the amino acid sequence, a yeast recombination gene engineering bacterial strain Pichia pastoris X-33/ pPIC6alpha/Pen with a preservation code of CCTCC No: M209126, as well as the utilization of the engineering bacterial strain to express the recombined litopenaeus setiferus protein SF-P9. Moreover, the invention also discloses a litopenaeus setiferus protein F-P6, a preparation method and an application thereof. Besides, the coding of an amino acid sequence of the litopenaeus setiferus protein Pen6 and a DNA sequence corresponding to the amino acid sequence is also disclosed in the invention. The recombined litopenaeus setiferus protein SF-P9 has enterokinase sites. After the process of enterokinase cutting on the litopenaeus setiferus protein SF-P9, the litopenaeus setiferus protein F-P6 is obtained. The litopenaeus setiferus protein SF-P9 and the litopenaeus setiferus protein F-P6 have obvious biological activities of antibacterial infection, antimycotics infection, and anti-virus infection, as well as have an obvious effect of inhibiting tumor cell growth. Besides, the litopenaeus setiferus protein SF-P9 and the litopenaeus setiferus protein F-P6 can be applied to prepare medicaments for treating or preventing bacteria infection, fungi infection and virus infection and to prepare antitumor drugs.

The invention relates to a disease-resisting and freshness-retaining treatment method for storage of picked mangoes at normal temperature, and belongs to the field of the fruit freshness keeping technology. The disease-resisting and freshness-retaining treatment method comprises the following steps: carrying out precooling on picked 70-80% matured mangoes, carrying out sterilization, blowing-drying or airing, soaking in hot water at 55 DEG C, fishing out, airing, uniformly spraying a mixed disease-resisting and freshness-retaining agent on the surface of the mangoes, airing, packaging and storing at normal temperature. According to the disease-resistant and freshness-retaining treatment method, diseases of the mangoes during storage at the normal temperature can be reduced effectively, the disease index and the diseased fruit rate of the mango are reduced remarkably, the commercial fruit rate of the mangoes is improved, the shelf life of the mangoes at the normal temperature is prolonged, the method can be operated conveniently and rapidly, the used disease-resisting and freshness-retaining agent is non-toxic and harmless, and the method can be promoted and applied easily.

The invention provides a plant-sourced medical fertilizer and a preparation method thereof and an application. The plant-sourced medical fertilizer is prepared from raw materials with specific components and specific dosages, and the preparation method of the fertilizer is simple in process and suitable for mass production. The plant-sourced medical fertilizer has medicine, fertilizer, nutrition and plant protection functions and belongs to no-harmful agriculture and organic agriculture materials. The plant-sourced medical fertilizer is black brown, free from extraneous odor and rich in nutritive substance, and the incidence rate and disease indexes of tobacco black shanks can be remarkably reduced. The plant-sourced medical fertilizer has the functions of facilitating root development, improving soil structures and providing soil enzyme activities. Pure-plant-sourced medical residues are fermented to obtain the plant-sourced medical fertilizer, any chemical agents cannot be added, environmental pollution is avoided, and the quality of the standard plant-sourced medical fertilizer completely reaches national no-harmful standards.

The invention belongs to microbial pesticide, in particular to a preparation method of fluorescent pseudomonas microbial pesticide. Rape sclerotiniose is one of the greatest diseases damaging the production and the yield of rapes, the prior art adopts agricultural prevention and treatment, chemical prevention and treatment and microbial pesticide prevention and treatment, but all the effects are unsatisfactory. The preparation method of the fluorescent pseudomonas microbial pesticide comprises the following steps: taking peptone, glycerol and the like to prepare a liquid culture medium; using a pipettor to inoculate a fluorescent pseudomonas P13 strain into the liquid culture medium and carrying out constant-temperature shaking culture; carrying out refrigerated centrifugation precipitation to prepare a P13 thallus; taking the peptone, the glycerol, agar and the like to prepare a solid culture medium; inoculating the P13 thallus into the solid culture medium and carrying out constant-temperature culture at 28 DEG C; adding the solid culture medium into a container with kieselguhr and stirring the mixture; and sealing the container and standing the container for 1 to 2 days at room temperature. The invention has the advantages of obvious effects of preventing and treating rape sclerotiniose, growth promotion for rapes, no toxic or side effect, good safety to people and livestock, simple preparation technique, low cost and simple and convenient use method.

The invention discloses a preparation method and application of Bacillus velezensis for efficiently antagonizing fusarium graminearum, and belongs to the technical field of microorganisms. The Bacillus velezensis NF002 disclosed by the invention can be used for efficiently antagonizing fusarium graminearum, can effectively inhibit generation of fusarium graminearum DON toxin at the same time, andcan degrade DON toxin in wheat grains. The Bacillus velezensis NF002 can be used as a biological prevention and treatment material for wheat scab, and has good application prospects in the aspect of development of new biopesticides or new biological prevention and treatment microbial agents.

Disclosed is a continuous-cropping bacteria resistant agent. The continuous-cropping bacteria resistant agent is composed of bacillus licheniformis bacteria powder, streptomyces microflavus powder, pythium oligandrum yeasts, green wooden mold powder, edible fungi residues, compound sodium nitrophenolate and alpha-naphthalene acetic acid. By the arrangement, continuous-cropping diseases of crops can be alleviated, diseases of bacterial wilt, tobacco mosaic and the like caused by continuous cropping are highly prevented, various diseases of thielariopsis basicola, blight, root-knot nematode, damping off and the like can be prevented, and incidence rate and disease index of flue-cured tobacco black shank disease, the thielariopsis basicola and common mosaic are effectively reduced; by the aid of the continuous-cropping bacteria resistant agent, flue-cured tobacco can be promoted in early growth and quick tiller, growth period is brought forward, plant height and stem thickness of the flue-cured tobacco are increased, yield of flue-cured tobacco leaves is increased, and appearance quality of the tobacco leaves is improved.

The invention discloses a Chinese herbal medicinal preparation for preventing and treating Chinese sacbrood. The preparation consists of the following crude medicines in parts by mass: 26-30 parts of longhairy antenoron herb, 26-30 parts of selfheal, 26-30 parts of plantain herb and 14-18 parts of acanthopanax root. Experiment proves that the Chinese herbal medicinal preparation for preventing and treating Chinese sacbrood, when being fed to Chinese bee swarm, is good in palatability and popular in the bee swarm, and the Chinese herbal medicinal preparation has no influence to the reproductive capacity of the bee swarm and can remarkably reduce the incidence and disease index of Chinese sacbrood; the Chinese herbal medicinal preparation is easy to keep, long in shelf life, simple in prepare, convenient and simple to apply, and can be industrially produced.

The invention provides a method for raising disease resistance of paddy rice to rice blast by the utilization of alginooligosaccharide. By spraying alginooligosaccharide during the rice seedling stage, activity of defensive enzymes--phenylalnine ammonialyase, catalase and peroxidase in the rice leaves is raised, and furthermore disease resistance of paddy rice to rice blast is enhanced. By the method, disease resistance of paddy rice is enhanced, and growth of paddy rice is promoted. Meanwhile, the method is simple and feasible and is beneficial to agricultural promotion and application.

The invention discloses a seed coating agent for preventing and treating cyst nematodes in wheat crops. The seed coating agent is characterized in that the raw materials contain methane dithiocyanate. The application method of the seed coating agent is characterized by airing and sowing seeds in natural state after coating seeds with the seed coating agent in an agent/seed ratio of 1:40-60 beforesowing the seeds of the wheat crops. The seed coating agent has the characteristics of safety for growth of the wheat crops, good adhesive property and long persistent period. The seed coating agent has obvious effect on controlling cyst nematodes in the wheat crops, can effectively reduce the base number of cyst nematodes in the fields and the disease index, improve the persistent period of the agent and increase the yield by 10-20% and has the advantages of safety, high efficiency, low toxicity, labor saving and economy.

The invention discloses an atractylodes macrocephala endophytic fungus and application thereof in preventing and treating root rot of atractylodes macrocephala, and belongs to the technical field of plant disease prevention and control. The atractylodes macrocephala endophytic fungus is obtained by separating and purifying the living body of atractylodes macrocephala of Atractylodes of composite family by using an endophytic fungus separation and purification technology, and is classified and named as Mortierella elongata AM478, and the culture preservation number is CGMCC No.17781. The invention also discloses the application of the endophytic fungus: the endophytic fungus can inhibit the growth of pathogenic bacteria of the root rot of the atractylodes macrocephala, induce the atractylodes macrocephala to generate systemic disease resistance, up-regulate the expression of the atractylodes macrocephala NBS-LRR-type disease resistance gene T7N9.24, and reduce the disease index of the root rot of the atractylodes macrocephala by 64.47%. The endophytic fungus of the invention has obvious prevention and treatment effect on the root rot of the atractylodes macrocephala, and brings wideapplication prospect for the field of biological prevention and treatment of the atractylodes macrocephala fungal diseases.

The invention discloses a disease preventing and freshness retaining method of mango-fruit preharvest and postharvest combined processing. The method includes the steps: (1), within 1 month before mango-fruit harvesting, a mixed disease-resistant pesticide is uniformly sprayed to the surfaces of mango fruits; (2), the mango fruits obtained after preharvest processing are harvested and precooled; (3), the precooled mango fruits are placed into a plastic box, a citronella essential oil, a tea tree essential oil, a lemon essential oil and a macadamia ternifolia essential oil are added into a culture dish at the bottom of the box so that the volatile matter concentration of each essential oil can reach 250-500 [mu]l/L, sealing is conducted, and room-temperature fumigation is carried out for 8-16 hours; (4), fumigated mango fruits are taken out and then placed into a paper fruit box with a spontaneous modified atmosphere bag as a lining, and after a 1-MCP slow-release agent is added, an opening of the modified atmosphere bag is sealed; and (5), the mango fruits are stored in a room-temperature warehouse. After the mango fruits processed through the method are stored in the room-temperature warehouse for 8 days, diseases are significantly alleviated, the disease index of the mango fruits is 20.0-31.7, and prevention and treatment effects reach 54.7-71.4%.