Bacillus halotolerans SY1836 and application thereof

A technology of salt-tolerant bacillus and bacteria agent, applied in the field of microorganisms, can solve problems such as soil salinization, groundwater pollution, lower pesticide utilization rate, and lower pepper quality, achieve good biological control effects, increase plant height, improve and fresh weight effect

Active Publication Date: 2020-11-24
重庆基微源生物技术有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The large-scale application of chemical pesticides has played an important role in improving the production of peppers, but it has also brought many problems, such as the development of drug resistance of pathogenic bacteria, the reduction of pesticide utilization, the decline of pepper quality, the increase of pesticide residues, soil salinization and groundwater pollution. A series of food safety and ecological environment issues

Method used

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  • Bacillus halotolerans SY1836 and application thereof
  • Bacillus halotolerans SY1836 and application thereof
  • Bacillus halotolerans SY1836 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] This example provides a process for isolating and purifying Bacillus halotolerans SY1836 from soil, specifically as follows:

[0027]Soil samples were collected from the salinized plot in Shuiyuan Town, Jinchang City, Gansu Province. Randomly select 5 points in the sampling plot, take 0-5cm salinized soil, bring it back in a sealed bag after mixing, remove soil clods and plant residues with a 0.2cm mesh sieve, and air dry at room temperature. Weigh 10g of soil sample into a conical flask filled with 90mL sterilized water, shake it for 10min, and make 10 -1 Concentration of soil suspension, let it stand until the soil particles settle, then draw 1mL supernatant and transfer it into a test tube filled with 9mL sterilized water to make 10 -2 Concentration of the soil suspension, followed by serial dilution to make the final concentration is 10 -5 soil suspension. choose 10 -3 , 10 -4 and 10 -5 200 μL of each of the suspensions of the three concentrations was drawn, a...

Embodiment 2

[0034] In this example, substances related to disease prevention and growth promotion of Bacillus halotolerans (Bacillus halotolerans) SY1836 are detected, and the specific method is as follows:

[0035] 1.1 Production of siderophore

[0036] Pick the strain and inoculate it on the CAS medium, place it in a 30°C incubator and cultivate it for 14 days, observe whether there is a yellow-orange circle, if there is, it means that the strain has the ability to produce siderophiles.

[0037] 1.2 Production of amylase

[0038] Pick the strain and inoculate it on the NA medium containing 1% soluble starch, culture it in a 30°C incubator for 5 days, observe whether there is a transparent circle, if there is, it means that the strain has the ability to produce amylase.

[0039] 1.3 Production of protease

[0040] Pick the strain and inoculate it on a casein yeast medium containing 7% skimmed milk powder (casein 5g, yeast extract 2.5g, glucose 1g, water 1000mL), place it in an incubato...

Embodiment 3

[0061] This embodiment further detects the greenhouse disease prevention and growth promotion effect of Bacillus halotolerans (Bacillus halotolerans) SY1836, the specific method is as follows:

[0062] 2.1 Preparation of Bacillus halotolerans SY1836 biocontrol agent

[0063] Bacillus halotolerans (Bacillus halotolerans) SY1836 was inoculated on LB solid medium for activation, then transferred to a 100mL Erlenmeyer flask containing 50mL of LB liquid medium, and cultured at 28°C for 24h with shaking at 150rpm to obtain seed liquid. The seed solution was inoculated in the fermentation medium (1 / 2PDB+1 / 2LB) at a ratio of 1:100, and cultured at 28°C with shaking at 150rpm for 24h.

[0064] 2.2 Determination of the seedling tray control effect of Bacillus halotolerans SY1836 on pepper blight

[0065] The seeds of pepper variety "Changfeng" were surface-sterilized in 0.1% sodium hypochlorite for 20 min, and then washed four times with sterile water. Seeds were sown in 50-well seedl...

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Abstract

The invention relates to bacillus halotolerans SY1836 and application thereof. The bacillus halotolerans SY1836 has an accession number of CGMCC No. 20080 and is a biocontrol bacterium separated and obtained from saline-alkaline soil. According to the application, verified by experiments, the bacillus halotolerans SY1836 has a relatively good effect of antagonizing various botanical pathogenic bacteria, can be used for effectively lowering a disease index of capsicum when the bacillus halotolerans SY1836 is applied to a growth process of the capsicum, and has a remarkable phytophthora blight control efficiency. The bacillus halotolerans SY1836 simultaneously can be used for remarkably improving plant height and fresh weight of the capsicum during growth and has an important significance inphytophthora blight prevention and control and planting of the capsicum.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a halotolerant bacillus SY1836 and its application. Background technique [0002] Capsicum is an annual or limited perennial herbaceous Solanaceae plant, which is an important vegetable and condiment. The seed oil is edible, and it also has certain medicinal value. With the continuous increase of the cultivation area of ​​pepper, the production is often accompanied by a high degree of intensive planting, resulting in a high multiple cropping index, which provides a host and breeding place for the occurrence of soil-borne diseases, resulting in a large accumulation of pathogenic bacteria in the soil. The occurrence of infectious diseases is becoming more and more serious, and the blight caused by Phytophthora capsici has seriously restricted the development of pepper production. [0003] At present, it is mainly controlled by breeding disease-resistant varieties and applyi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00A01P21/00C12R1/07
CPCA01N63/22C12R2001/07C12N1/205
Inventor 徐生军荆卓琼郭致杰张新瑞
Owner 重庆基微源生物技术有限责任公司
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