14 results about "Chlamydospore" patented technology

The present invention refers to a coating composition comprising a suspension of a microorganism, wherein the suspension comprises a pigmented vegetative cell, an unpigmented vegetative cell and a pigmented chlamydospore of the microorganism, and a surfactant ranging from 130 g / mol and 1500 g / mol. The invention further relates to a method for the preparation of the coating composition, a method for application coating one or more coating pre-layers and curing a material, a coated material obtainable by this method and a method for refreshing the coating of the coated material.

The invention provides a DSE chlamydospore water aqua as well as a preparation method and application thereof, and belongs to the technical field of microbial preparations, the DSE chlamydospore water aqua comprises chlamydospore from at least one dark partitioned endophytic fungus, and further comprises 5g / mL-10g / mL of preservative and 5g / mL-10g / mL of protective agent, and the content of the chlamydospore is greater than 2.5 * 10 < 8 > CFU / mL. The product property of the water aqua is improved by reasonably optimizing the dosage of DSE chlamydospore and auxiliary materials, the content of chlamydospore in the obtained water aqua finished product can reach 2.54 * 10 < 8 > CFU / mL, and all indexes meet the national standard; the conidium water aqua can be stored for more than 12 months at normal temperature, and the shelf life is prolonged by more than 9 months compared with a conventional conidium water aqua. The DSE chlamydospore aqueous solution can effectively prevent and treat tomato bacterial wilt and southern rice black-streaked dwarf disease, improve the growth vigor of tomato plants and rice plants, and achieve a prevention and treatment effect which is not inferior to or even superior to that of positive control trichoderma harzianum. The DSE chlamydospore aqueous solution provided by the invention is simple to prepare, controllable in cost and excellent in performance, and is suitable for large-scale popularization and application as a novel biopesticide product.

The application discloses a method for inducing differentiation of Trichoderma to produce chlamydospores, and relates to the field of agricultural biotechnology. The method comprises the following steps: adding an inducing substance to a culture medium during fermentation culture to induce differentiation of Trichoderma to produce chlamydospores; and the inducing substance comprises at least one of ammonium persulfate, ammonium bisulfate, ammonium chloride or ammonium bromide. By adding the inducing substance at the beginning of fermentation or in the middle of fermentation, the spore type of Trichoderma can be changed from conidium to chlamydospore, so that the proportion of chlamydospores in the Trichoderma biocontrol preparation can be improved. Therefore, the method has important significance for solving the problem of short shelf life of Trichoderma. The method has a significant inducing effect in various culture media and various specifications of bioreactors, the inducing substance is low in price, simple in operation during use, suitable for large-scale production of various scales, and has a wide market prospect.

The invention provides a non-classical terpenoid cyclase for biosynthesis of trichoderma diterpenoid compounds harzianol I and wickerol A, a coding gene of the non-classical terpenoid cyclase, and a biological function of the non-classical terpenoid cyclase and the harzianol I for regulating and controlling a fungal resistant propagule. Belongs to the technical field of natural product biosynthesis. The non-classical terpenoid cyclase TriDTCs disclosed by the invention is a brand new terpenoid synthase family, and can be used for catalyzing a diterpene biosynthesis precursor GGPP to synthesize framework precursor compounds harzianol I and wickeranol A of trichoderma specific diterpenoid trichoderma harzianum and trichodermin; in addition, the TriDTCs and the harzianol I have the biological functions of regulating and controlling the generation of trichoderma fungus chlamydospore and aspergillus oryzae nucleus. According to the invention, an efficient biosynthesis method is provided for heterologous synthesis of harzianol I and wickerol A, and biological and chemical ways are provided for enhancing generation of resistant spores of trichoderma and aspergillus oryzae.

This invention provides a wettable powder of DSE (dichloropyrophylloides) spores, its preparation method, and its application, belonging to the field of microbial preparation technology. It comprises chlamydospores from at least one dark-colored septate endophytic fungus, a carrier, and excipients, wherein the chlamydospore content is greater than 2.35 × 10⁻⁶. 8 CFU / g. This invention improves the properties of wettable powder by rationally optimizing the dosage of DSE chlamydospores and excipients, achieving a chlamydospore content of 2.35 × 10⁻⁶ CFU / g in the finished product. 8 With a concentration of CFU / g, a wetting time of 24.25s, and a suspension rate of 73.8%, all indicators meet national standards. It can be stored at room temperature for over 12 months, extending its shelf life by more than 6 months compared to conventional conidiophore agents. This powder significantly increases the expression of five defense enzymes—PAL, PPO, POD, CAT, and SOD—in tomato and rice plants, thereby activating the plant's defense system. It effectively controls bacterial wilt in tomatoes and black-streaked dwarf disease in southern rice, improving the growth of tomatoes infected with bacterial wilt and rice infected with black-streaked dwarf disease. It is suitable for promotion and use as a novel biological pesticide product.

The invention belongs to the technical field of biological prevention and control of plant diseases, and particularly relates to a biological source rhizosphere metabolite phthalic acid and application thereof in prevention and control of sugarcane smut. The invention relates to an application of a biological source rhizosphere metabolite in prevention and control of sugarcane smut. The biological source rhizosphere metabolite is phthalic acid. The key rhizosphere metabolite phthalic acid with efficient and targeted bacteriostatic activity is screened and identified from disease-inhibiting soil, safe and efficient prevention and control of sugarcane smut are achieved by targeted inhibition of germination of pathogenic bacteria chlamydospore, and the key rhizosphere metabolite phthalic acid can be developed as a biological source pesticide and has broad application prospects. And the method has great significance on pesticide reduction, synergism and green development of agriculture.

The present application belongs to the field of microbial technology, and particularly relates to a method for producing chlamydospores of dark septate endophytic fungus Phialophora fastigiata by liquid fermentation. The present application finds out the culture medium and culture conditions suitable for the growth of Guangxi Phialophora fastigiata HX2 through single factor experiment, response surface optimization experiment and the like. After optimization, the method greatly improves the yield of chlamydospores produced by Phialophora fastigiata, effectively reduces the production cost of chlamydospores, and prolongs the shelf life of the microbial agent. According to the calculation, the yield of chlamydospores is increased by 139.8% compared with the traditional PDB culture medium, the cost is reduced by 47%, and the shelf life is prolonged by about 8 months. The utilization of the growth-promoting and immunity-inducing strain HX2 is effectively improved. The culture medium is simple to prepare, the fermentation process is stable, and the method is suitable for large-scale production. The prepared chlamydospore preparation has strong stress resistance and long shelf life, and provides a new effective way for the industrialization of the Phialophora fastigiata strain in the later stage.

The invention provides a strain of bacillius pink and application thereof in promoting formation of sporangia branches of porphyra haitanensis. The preservation number of the provided bacillius pink is CCTCC (China Center for Type Culture Collection) NO: M 20242610. According to the method, the pink rod-shaped strain is added into a porphyra haitanensis protonema culture system, so that the development of the sporangium branches can be effectively promoted, the formation proportion of the sporangium branches can be remarkably increased to 48.8% after culture is performed for 4 weeks, directional ripening under manual intervention is realized, the defects of long period and poor stability of a traditional environment regulation and control means are overcome, and the method is suitable for large-scale popularization and application. The conchospore diffusion consistency and quality can be improved, and a new technical approach is provided for porphyra haitanensis seedling production.

The invention provides DSE chlamydospore wettable powder as well as a preparation method and application thereof, and belongs to the technical field of microbial preparations, the DSE chlamydospore wettable powder comprises chlamydospore from at least one dark partitioned endophytic fungus, a carrier and auxiliary materials, and the content of the chlamydospore is greater than 2.35 * 10 < 8 > CFU / g. The product property of the wettable powder is improved by reasonably optimizing the dosage of the DSE chlamydospore and the auxiliary materials, the chlamydospore content in the finished product can reach 2.35 * 10 < 8 > CFU / g, the wetting time is 24.25 s, the suspension rate is 73.8%, and all indexes meet the national standard; the product can be stored for more than 12 months at normal temperature, and the shelf life is prolonged by more than 6 months compared with that of a conventional conidium microbial agent. The powder can remarkably improve expression of five defensive enzymes, namely PAL, PPO, POD, CAT and SOD, in tomato and rice plants, so that a defensive system of the plants is activated, tomato bacterial wilt and southern rice black-streaked dwarf can be effectively prevented and treated, the growth vigor of the tomato infected with the bacterial wilt and the growth vigor of the rice plants infected with the southern rice black-streaked dwarf are improved, and the powder is suitable for being used as a novel biopesticide product to be popularized and used.

The application discloses a method for reversibly inhibiting the maturation of a Porphyra haitanensis filament, and belongs to the technical field of Porphyra haitanensis breeding. The method comprises adding DMTU to a filament culture medium, and provides a method for inhibiting the maturation of a Porphyra haitanensis filament. When maturation is started, the filament culture solution containing DMTU is used to promote the maturation of the filament, so that the growth of the Porphyra haitanensis filament and the formation of sporangial branches can be effectively inhibited. The inhibiting effect is reversible, and normal maturation can be restored after the treatment is stopped. The inhibiting effect is immediately released, and then the precise regulation of the maturation process of the filament is realized, and the large number of "miscarriages" of chlamydospores is effectively prevented. Therefore, the maturation inhibition method has a good application prospect, and has important practical significance and application value for improving the technical level of the Porphyra haitanensis breeding industry and guaranteeing the sustainable development of the industry.

The application discloses a new rice variety breeding method for resisting rice smut and a misting inoculator, relates to the technical field of rice breeding, and comprises the following steps: (1) hybrid breeding of rice smut-resistant rice varieties; (2) single-plant selection of resistant varieties in a disease nursery; (3) rice smut resistance identification through misting inoculation; and (4) quality trait analysis to obtain a new rice variety resistant to rice smut and having high quality. The application screens rice varieties resistant to rice smut through multi-generation hybrid breeding and single-plant selection under natural disease infection conditions. The misting inoculation method is adopted to uniformly distribute rice smut chlamydospores in the air, and then the rice is contacted with the air, and the disease resistance is evaluated through moisture and heat preservation treatment. The application can efficiently obtain a new rice variety resistant to rice smut and having high quality, can realize improvement of inoculation controllability, cost saving, uniform inoculation, stable effect, enhancement of the anti-infection capacity of the new variety, and can breed a new rice variety with early maturity and high yield.

The invention belongs to the technical field of agricultural ecological cultivation and plant protection, and discloses a method for reducing fresh pea root rot in a flue-cured tobacco-fresh pea rotation mode based on synergism of rotation and a microbial agent. The corn is used for replacing the third-year Dahun flue-cured tobacco; in the third year, when the spring crop corn is sown, a Purpureocillium lilacinum and Trichoderma asperellum complex microbial inoculant is applied in a matched manner; after garlic bulbs are harvested, healthy straw and root system residues are smashed and returned to the field, soil humidity is kept, and biological fumigation is carried out; after the corn season, straw is smashed, deeply ploughed and returned to the field. The three measures have a synergistic effect to jointly strengthen the inhibition and removal functions on the pathogenic bacterium dormant bodies of the pea root rot in the soil, and the micro-ecological health of the soil is remarkably improved, so that strong root disease system resistance is provided for later fresh peas. On the premise that an original crop rotation system framework is not damaged, green and efficient ecological prevention and control of soil-borne diseases are achieved.

The present application belongs to the field of microorganism and agricultural waste recycling technology, and relates to a Trichoderma agent, a bio-organic fertilizer and a preparation method and application thereof. A mint processing waste is used as a raw material, and liquid fermentation is carried out by using Trichoderma harzianum ZL-811, and then the fermented liquid is mixed with a culture medium carrier to obtain the product. The present application can carry out high-density fermentation of Trichoderma, obtain a fermentation product containing a large number of chlamydospores, prepare a Trichoderma agent, and then add a matured organic fertilizer to produce a multifunctional Trichoderma bio-organic fertilizer. Not only the resource utilization of the mint processing waste is realized, but also the Trichoderma agent and the multifunctional bio-organic fertilizer produced can be used for field application.

This invention provides a DSE (dichloropyrophyll-forming element) aqueous solution, its preparation method, and its application, belonging to the field of microbial preparation technology. It comprises chlamydospores from at least one dark-colored septate endophytic fungus, and also includes 5 g / mL to 10 g / mL of preservative and 5 g / mL to 10 g / mL of protective agent, wherein the chlamydospore content is greater than 2.5 × 10⁻⁶. 8 CFU / mL. This invention improves the properties of aqueous solutions by rationally optimizing the dosage of DSE chlamydospores and excipients, resulting in an aqueous solution with a chlamydospore content of 2.54 × 10⁻⁶ CFU / mL. 8 The concentration of CFU / mL meets national standards; it can be stored at room temperature for more than 12 months, extending its shelf life by more than 9 months compared to conventional conidial aqueous solutions. This DSE chlamydospore aqueous solution effectively controls bacterial wilt in tomatoes and black-streaked dwarf disease in southern rice, improving the growth of tomato and rice plants, and achieving control effects comparable to or even superior to the positive control *Trichoderma harzianum*. The DSE chlamydospore aqueous solution provided by this invention is simple to prepare, cost-effective, and has excellent performance, making it suitable for large-scale promotion and use as a novel biological pesticide product.