This invention belongs to the field of
biotechnology-assisted breeding and discloses a SNP marker for identifying resistance to
bacterial soft rot in cucumber and its application. The SNP
molecular marker of this invention is located at physical positions 13,925,944 bp on cucumber
chromosome 2. Cucumber materials with a base of G at this site are resistant to
bacterial soft rot, while cucumber materials with a base of A at this site are susceptible to
bacterial soft rot. Using the SNP marker provided by this invention, the resistance of cucumber plants to bacterial soft rot can be rapidly and accurately identified during the
seedling stage, enabling high-
throughput screening of a large number of breeding materials. This method has advantages such as high detection efficiency and no limitation by environmental conditions, significantly improving the selection efficiency in
disease-resistant breeding, shortening the breeding cycle, and providing an effective tool for marker-assisted selection breeding of cucumbers resistant to bacterial soft rot.