49 results about "Bacterial soft rot" patented technology

The invention relates to bacillus subtilis M3 and an application thereof, and belongs to the technical field of biological pesticides. The produced strain is the bacillus subtilis M3; the preservation unit is the China General Microbiological Culture Collection Center; the address is Zhongguancun, Beijing, China; the preservation date is July 11, 2013; the preservation number is CGMCC NO.7911. The application of the bacillus subtilis M3 as a preparation for preventing and controlling Chinese cabbage clubroot, tomato scab, tomato root knot nematode diseases and konjak bacterial soft rot is also disclosed. The bacillus subtilis M3 disclosed by the invention achieves an obvious and stable prevention and control effect on the diseases, can be used for preparing the preparation for preventing and controlling the Chinese cabbage clubroot, the tomato scab, the tomato root knot nematode diseases and the konjak bacterial soft rot and has the advantages of high efficiency, nontoxicity, safety, no residue, bacteria activation, disease control, better growth promotion effect, better comprehensive character and easiness for industrialized production.

The invention discloses a compound biological preservative. The compound biological preservative comprises the components as follows: 0.1-5 g of component B is added in each 1L of component A, the component A is fermentation liquor of Bacillusamyloliquefacienssubsp.plantarum BGP20 strains, and the component B is natamycin. The compound biological preservative is used for preventing and controlling diseases of fruits and vegetables in a storage period and particularly can be used for simultaneously preventing and treating bacterial soft rot and various fungal diseases (for example, phytophthora capsici, rhizopus soft rot, pythium blight and the like) of fruits and vegetables in the storage period, avoiding or reducing residual chemical pesticides and agricultural antibiotics and maintaining the quality of the fruits and vegetables in the storage period, so that the compound biological preservative has good economic and social benefits.

The invention relates to a Konjak endophytic bacteria Pantoea agglomerans bacterial strain1-7 and an application, which belongs to the biology technical field. The Pantoea agglomerans1-7 is preserved in the China General Microbiological Culture Collection Center on May 28th, 2012 with the preservation number of CGMCC No.6160. The Konjak endophytic bacteria Pantoea agglomerans bacterial strain1-7 is used for preparing the preparations used for preventing and treating bacterial soft rot of konjak and clubroot disease on cruciferous vegetable club root in fields. The Konjak endophytic bacteria Pantoea agglomerans bacterial strain1-7 has the advantages of high efficiency, no toxicity, safety and no residue, the bacterial strain1-7 has the characteristics of good disease control, better growth promotion effect, better comprehensive characters and easy industrial production, and the Pantoea agglomerans1-7 can be applied to preparation of preparations for controlling cruciferous vegetable club root in fields.

The invention discloses a method for promoting orchid to grow and preventing diseases, and relates to a method for preventing the diseases and promoting orchid branches to grow and prolonging a flowering phase. The method disclosed by the invention comprises the following steps of uniformly mixing a solid state culture and a culture medium into a basin according to the mass ratio of 1 to 50 in orchid plant transplantation, wherein the solid state culture has the spore concentration s 1*10<7>-1*10<8> spore/g and prepared from Trichoderma harzianum T216, and planting an orchid plant, wherein the solid state culture can induce the resistance of the orchid plant to the fungoid diseases such as southern blight, rhizoctonia solani and anthracnose and bacterial soft rot caused by Erwinia carotovara during orchid growth, the plant morbidity is reduced, the disease index is reduced, the orchid is promoted to grow in advance, and the number of blades of the orchid are increased. In addition, the method disclosed by the invention has the advantages that a liquid state culture of the Trichoderma harzianum T216 is regulated into 1*10<5>-1*10<7> spore/mL when the orchid peeps a bud, and the spray treatment is carried out on the orchid plant, so that the branch number of the orchid is increased, and the flowering phase of the orchid is prolonged.

The invention discloses pseudomonas fluorescens SC3 and application thereof in preventing and controlling bacterial soft rot of crops. The pseudomonas fluorescens SC3 is collected in the Guangdong Microbial Culture Collection Center, with the collection number GDMCC NO. 60367, at the date of April, 30th, 2018. The The pseudomonas fluorescens SC3 has remarkable suppression effect on various bacterial diseases, especially on bacterial soft rot of crops, ralstonia solanacearum and xanthomonas, reduces incidence rate of bacterial soft rot of crops, and has good prevention and control effect in potted experiments. Besides, the pseudomonas fluorescens SC3, which is avirulent, can be used for development of biopesticide for treating crop bacterial diseases and provides a new thought and idea about biological prevention and control of bacterial soft rot of crops.

Transgenic plants having resistance against bacterial soft rot diseases, harboring the bacterial autoinducer inactivation gene encoding (AiiA) protein, and methods for protecting plants from bacterial pathogens by transforming the plants with the aiiA gene are disclosed.

The invention discloses a bacillus amyloliquefaciens strain and application thereof in prevention and control of celery bacterial soft rot. The bacillus amyloliquefaciens refers to bacillus amyloliquefaciens ZF75 whose preservation number is CGMCC No.16777 in the China General Microbiological Culture Collection Center. The bacillus amyloliquefaciens ZF75 is a celery soil rhizospheric microorganismwhich is safe to humans, animals and crops and environmental-friendly. The bacillus amyloliquefaciens strain has strong antagonism to the celery bacterial soft rot. The bacillus amyloliquefaciens strain is adopted for biocontrol of the celery bacterial soft rot, which is an effective and stable biocontrol manner for the celery bacterial soft rot, the characteristics that the bacillus amyloliquefaciens strain is prone to acting on celery plants and has strong antagonism to the celery bacterial soft rot are made use of, and the requirement on celery bacterial soft rot prevention and control inagricultural production can be satisfied better.

The invention discloses a method and applications for fast detecting pathogenic bacteria molecules of bacterial soft rotting disease for banana. The method comprises the steps that: a specific primer is utilized to conduct PCR (polymerase chain reaction) on DNA contained in a sample, and the sample contains the pathogenic bacteria molecules of bacterial soft rotting disease for banana when the PCR product presents a 171bp condition. The method is simple to operate, takes less time and is large in flux. The method is used for conducting determined detection to banana seedlings, soil leach liquor of soil in a planting area, irrigation water and the like, thus realizing the purposes of construction and safety production in no disease banana seedling production base in China and simultaneously stopping of introduction of dangerous foreign disease banana.

The invention relates to a lysobacter antibioticus HY strain and application thereof and belongs to the technical field of plant protection. The lysobacter antibioticus HY strain has already been preserved in the China General Microbiological Culture Collection Center on July 11, 2013 and the preservation number is CGMCC No.7912. The konjak rhizosphere bacteria lysobacter antibioticus HY strain is used for preparation of a medicine used for preventing and treating brassicaceous vegetable clubroots, tobacco black shanks and konjak bacterial soft rots in fields. The lysobacter antibioticus HY strain is efficient, non-toxic, safe, free of residue, capable of achieving the disease-control purpose by means of live bacteria, good in plant growth promoting effect, good in comprehensive performance, and suitable for industrialized production, bulk production and application.

The invention discloses a strain of Pseudomonas putida BC10 and application thereof in controlling bacterial soft rot of crops. The strain was collected in the Guangdong Microbiological Culture Collection Center on July 10, 2018, and the collection number is GDMC NO.60411. The strain has a remarkable inhibitory effect on various bacterial diseases, especially has a good inhibitory effect on rice bacterial foot rot, bacterial soft rot pathogens of banana and soft rot pathogens of kaffir lily, can reduce bacterial soft rot of crops, and has a good control effect in pot experiments. The strain BC10 has no pathogenicity itself. It shows that the strain can be used for developing biopesticides for bacterial diseases of crops.

The invention discloses a primer group for detecting field pectobacterium carotovorum subsp and a kit and method for extracting and detecting DNA of the pectobacterium carotovorum subsp. The kit combines a sample nucleic acid rapid extracting technology and a loop-mediated isothermal amplification technology to establish a bacterial soft rot pathogenic bacterium product based on a soft rot pathogenic bacterium specific target single-base difference phenomenon. The detection method has the advantages that soft rot pathogenic bacteria, plant tissue and soil samples can be rapidly detected separately, nucleic acid extraction can be completed within 30 seconds, detection only needs 30-40 minutes, detection is rapid and accurate, no expensive instrument is needed, the cost is low, and the detection method can be directly used for real-time and on-site detection in the field, which facilitates dynamic monitoring of the soft rot pathogenic bacteria in soil, facilitates the development of popular prediction and early warning work of the soft rot, and facilitates early disease prevention and treatment.

The invention discloses Lactobacillus casei WX322 with a preservation number of CGMCC No.17710, a strain and its metabolites have broad-spectrum antibacterial activity, which can exhibit strong antibacterial ability against a variety of Gram-positive and Gram-negative bacteria (including pathogenic and spoilage bacteria), can be used to prepare antibacterial or/and antiseptic products, and can beused in the fields of medical, food and other industry to control bacterial pathogens or spoilage bacteria, can also effectively inhibit the growth of the soft rot pathogen Pectobacterium carotovorum,and can be used to prepare products for controlling bacterial soft rot of vegetables, can control the decay of vegetables caused by Pectobacterium carotovorum before and after harvest, can effectively solve the problem of agricultural residues and environmental pollution caused by pesticide control, has good application potential in the biological control of vegetable bacterial soft rot, and alsoprovides an excellent basic strain for the development of microbial pesticides.

The invention discloses an application of levofloxacin hydrochloride in preparation of a pesticide preparation for controlling banana bacterial soft rot. Levofloxacin hydrochloride can be used for preparing soluble powder for controlling banana bacterial soft rot; the levofloxacin hydrochloride soluble powder is prepared from the following components in percentage by mass: 50%-70% of levofloxacinhydrochloride, 10%-20% of soluble filler and 10%-40% of an aid. The filler and the aid are added to the levofloxacin hydrochloride raw medicine, the mixture is stirred and mixed uniformly and then ispulverized by a pulverizer, and the levofloxacin hydrochloride soluble powder is obtained. The levofloxacin hydrochloride soluble powder has rapid bactericidal effect on bacteria of banana bacterial soft rot, and can be sprayed in high-temperature and high-humidity seasons to prevent diseases; or, the levofloxacin hydrochloride soluble powder is sprayed in the initial stage of banana bacterial soft rot to prevent occurrence and spread of the disease, the control effect on the disease is good, and accordingly, a new storage technology is provided for control of banana bacterial soft rot.

The invention discloses farm chemical granules. The farm chemical granules are prepared from, by weight, 3-4.8 parts of carbendazim, 2 parts of buprofezin, 0.1-2 parts of stabilizing agents, 0.1-2 parts of synergist, 1-5 parts of dispersant, 1-10 parts of agglomerant, 0.1-1 part of colouring agents and 73.2-92.7 parts of carriers. The invention further provides a preparation method of the farm chemical granules. The farm chemical granules and the preparation method thereof have the advantages that a concave-convex rod is adopted as a farm chemical carrier, low-toxicity and low-residue bactericide, namely the carbendazim and the buprofezin, is mixed with the agglomerant and auxiliary agents in proportion, the granules are prepared and obtained, the detect that common granules are short in persistent period is overcome, and certain fertilizer efficiency, soil improvement property and environmental protection property are further achieved. Granule farm chemical prepared through the method has the advantages of being great in target property, low in manufacturing cost, high in chemical adsorption rate and long in persistent period, and a good prevention and control effect is achieved on onion root rot and bacterial soft rot caused by fusarium oxysporum.

The invention relates to a tendril-falling method for preventing stems of melon vegetables from bacterial soft rot. The method is adopted in a position-changing tendril-falling manner. Therefore, a tendril can fall at 50-70cm. During tendril-falling process, stems are prevented from distortion and cracks so that no wound occurs. Therefore, stems can grow naturally and straightly. Additionally, upper parts of plants can change positions on a same ridge and between double rows. Therefore, temperature and illumination in the interior of equipment are uniformly enjoyed. Therefore, plants are uniform.

The invention provides a dinotefuran and copper calcium sulphate suspension emulsion and application thereof, relating to a suspension emulsion containing active ingredients including dinotefuran and copper calcium sulphate, wherein the weight ratio of dinotefuran to copper calcium sulphate ranges from 1:50 to 50:1. The invention also relates to application of combination of dinotefuran and copper calcium sulphate to prevention or control of citrus canker, bacterial blight of rice and bacterial soft rot of vegetables. Dinotefuran and copper calcium sulphate are compounded to achieve synergism, the fungicidal effect of the composition is remarkably improved compared with that of a single dosage agent, the suspension emulsion is safe to human and livestock, the probability of drug resistance of pests is low, the dosage and the cost of the pesticide are reduced and the environment damage is alleviated.

The invention discloses a method for preventing and controlling phalaenopsis bacterial soft rot through environment regulation. The method includes: putting cupped seedlings in environment with highest value of day temperature ranging from 25 DEG C to 27 DEG C, highest value of night temperature ranging from 22 DEG C to 24 DEG C and highest value of relative air humidity ranging from 65% to 70% for culture. By controlling highest day temperature, highest night temperature and highest humidity value of a greenhouse, incidence rate of phalaenopsis bacterial soft rot is lowered, and the method ispollution-free, low in cost and good in effect.

The invention discloses a sterilization composition with benziothiazolinone and oxolinic acid and application. The sterilization composition comprises effective components of the benziothiazolinone and the oxolinic acid, and the mass ratio of the benziothiazolinone to the oxolinic acid is (60-1):(1-60). The sterilization composition can be applied to prevention and control over various bacterial diseases of fruit trees, vegetables and cereal crops and has an obvious synergistic interaction function, the pesticide resistance of pathogenic bacteria is delayed, the lasting period is long, the pesticide application frequency and pesticide application amount are reduced, and the application cost is lowered. The sterilization composition can be applied to prevention and control over diseases such as cucumber bacterial angular leaf spot, pseudomonas solanacearum, bacterial soft rot of potatoes, bacterial angular leaf spot of mangoes, bacterial blight of rice, citrus canker, canker of pears and bacterial canker of kiwifruit and has an obvious effect.