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Pseudomonas fluorescens SC3 and application thereof in preventing and controlling bacterial soft rot of crops

A Pseudomonas fluorescens, bacterial technology, applied to Pseudomonas fluorescens SC3 and its application in the prevention and control of bacterial soft rot of crops, can solve the problem of unconducted field tests, water pollution, and bacterial resistance. and other problems, to achieve the effect of reducing the occurrence of bacterial soft rot of crops, good control effect and good inhibitory effect

Active Publication Date: 2018-10-09
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, some studies have pointed out that Hydramycin and Junduqing can prevent and control bacterial soft rot diseases of bananas, but no field tests have been carried out.
However, chemical agents have certain toxicity to humans and animals, and can also cause problems such as bacterial resistance and water pollution.

Method used

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  • Pseudomonas fluorescens SC3 and application thereof in preventing and controlling bacterial soft rot of crops
  • Pseudomonas fluorescens SC3 and application thereof in preventing and controlling bacterial soft rot of crops
  • Pseudomonas fluorescens SC3 and application thereof in preventing and controlling bacterial soft rot of crops

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Isolation and Screening of Pseudomonas SC3

[0037] 1. Strain isolation

[0038] Sample collection: In the rice fields of Sichuan Agricultural University, the plants with rotten bases of rice seedlings were pulled out, together with soil samples from the roots of the rice, stored in bags, and brought back to the laboratory for strain isolation.

[0039] Isolation of bacterial strains: Soil samples were isolated by dilution coating plate method. Specifically, after the collected soil samples were thoroughly mixed by hand, 4.0 g was weighed, soaked in 20 mL of sterile water and shaken, and incubated at 28°C for 20 min on a shaker at 180 rpm for gradient dilution. Take the dilution factor as 10 5 -10 9 100 µL of the diluted solution was spread on the LB plate, air-dried at room temperature, sealed, and incubated upside down in a 28°C incubator for 24 h. Pick a single colony grown on the LB plate, label it, place it in a 2 mL centrifuge tube containing 500 µL ...

Embodiment 2

[0041] Example 2 Identification of Pseudomonas SC3

[0042] 1. Morphological identification

[0043]Strain SC3 is Gram-negative; the colonies on LB nutrient medium are light yellow, round, slightly convex in the middle, smooth in surface and neat in edge; diffuse turbidity in liquid medium, liquefaction of gelatin, production of acid, gas, Produces oxidase, catalase, and no amylase.

[0044] 2. Molecular identification

[0045] In order to clarify the taxonomic status of the strain SC3 obtained in Example 1, we used the method of multilocus sequence analysis (Multilocussequences analysis, MLSA) combined with phylogenetic analysis (Phylogenetic analysis) to identify the SC3 strain.

[0046] 16S rDNA sequence analysis is a molecular biological identification method for bacterial identification. The present invention analyzes the 16S rDNA sequence and combines the conservative housekeeping gene gyrB , wxya and wxya sequence, using MEGA 5.0 software to construct a phylogen...

Embodiment 3

[0048] Example 3 Determination of Pseudomonas SC3 Antibacterial Spectrum

[0049] 1. In order to better study the biocontrol potential of bacterial strain SC3 of the present invention, we have studied the bacteriostatic spectrum of this bacterium. The specific operation is as follows:

[0050] (1) To explore whether SC3 has the ability to inhibit the growth of other pathogenic bacteria. Use the method identical with embodiment 1 bacterial strain screening, change pathogenic bacterium MS2 into the equal amount of rice base rot pathogen EC1, banana bacterial soft rot pathogen MS2 and MS3, bacterial wilt pathogen EP1 (first Replace 15 mL of LB with 15 mL of TTC), Brassicaceae black rot fungus Xcc, rice bacterial blight Xoo, peach rot fungus Pantoea ananatis PP1, yellow skin rot pathogen Pantoea anthophila CL1, the rest of the steps remain unchanged.

[0051] (2) To explore whether SC3 has the ability to inhibit the growth of pathogenic fungi. The fungi tested included caps...

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Abstract

The invention discloses pseudomonas fluorescens SC3 and application thereof in preventing and controlling bacterial soft rot of crops. The pseudomonas fluorescens SC3 is collected in the Guangdong Microbial Culture Collection Center, with the collection number GDMCC NO. 60367, at the date of April, 30th, 2018. The The pseudomonas fluorescens SC3 has remarkable suppression effect on various bacterial diseases, especially on bacterial soft rot of crops, ralstonia solanacearum and xanthomonas, reduces incidence rate of bacterial soft rot of crops, and has good prevention and control effect in potted experiments. Besides, the pseudomonas fluorescens SC3, which is avirulent, can be used for development of biopesticide for treating crop bacterial diseases and provides a new thought and idea about biological prevention and control of bacterial soft rot of crops.

Description

technical field [0001] The invention belongs to the technical field of plant disease biocontrol. More specifically, it relates to a strain of Pseudomonas fluorescens SC3 and its application in controlling bacterial soft rot of crops. Background technique [0002] Bacterial diseases are plant diseases caused by bacterial infection, such as soft rot, bacterial wilt, etc., causing serious economic losses. Symptoms of bacterial diseases are wilting, rotting, withering, etc. In the later stage of the disease, in wet weather, bacterial mucus overflows from the diseased part, and there is an obvious foul smell, which is the characteristic of bacterial diseases. [0003] For example, D. cornella ( Dickeya zeae ), the fungus has a wide range of hosts. In addition to infecting monocotyledonous plants, it can also infect dicotyledonous plants at the same time. Grass, pineapple, potato, tobacco, Chinese cabbage, carrot, peanut, impatiens, chrysanthemum, etc. reported by D. zeae Th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P1/00C12R1/39
CPCA01N63/00C12N1/205C12R2001/39
Inventor 周佳暖李洁玲胡明张炼辉
Owner SOUTH CHINA AGRI UNIV
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