55 results about "Bacterial blight" patented technology

The invention discloses pseudomonas chlororaphis S7-24 for antagonizing xanthomonas oryzae and application of the pseudomonas chlororaphis S7-24, and belongs to the technical field of microorganisms. The pseudomonas chlororaphis S7-24 is preserved in the China Center for Type Culture Collection on November 12, 2025, and the preservation number of the pseudomonas chlororaphis S7-24 is CCTCC NO: M 20252533. The pseudomonas chlororaphis S7-24 provided by the invention is a newly found pseudomonas biocontrol resource, and verification shows that the strain S7-24 has relatively high antagonistic activity on rice bacterial blight and rice bacterial streak, and can be used for effectively preventing and treating rice bacterial blight and rice bacterial streak. The pseudomonas chlororaphis S7-24 provided by the invention is used for preventing and treating rice bacterial leaf blight and rice bacterial leaf streak, can reduce the use amount of chemical pesticides, ensures grain safety, and has better economic and ecological benefits.

The invention relates to the technical field of agricultural disease detection and crop disease resistance screening, in particular to a rice bacterial leaf blight disease resistance screening method based on an unmanned aerial vehicle and deep learning, and adopts the technical scheme that an optimized YOLOv11-OBB model is composed of a C3k2FC module, an SPPFLSKA module, a SlimNeck module and a LiteHead module, is responsible for extracting features in an image and positioning bacterial leaf blight spots, and is used for screening the disease resistance of the rice bacterial leaf blight; bacterial leaf blight can be efficiently and accurately detected; a lightweight deep learning model is used, efficient operation on a resource-limited unmanned aerial vehicle platform can be realized, and the equipment and calculation cost is reduced; through the cooperation of a plurality of optimization modules, the detection precision of bacterial leaf blight spots is remarkably improved, and a stable detection effect can be kept; the operation process is simplified, and full automation of bacterial blight disease resistance screening is realized; therefore, low-cost, efficient, automatic and high-precision bacterial blight disease resistance screening is realized by utilizing the lightweight and optimized YOLOv11-OBB model and combining module design with high calculation efficiency, and the method is suitable for large-scale field application.

The invention provides an application of a Ustilaginoidea virens UvWSC1 gene in improvement of plant disease resistance. The invention develops a new application of the Ustilaginoidea virens UvWSC1 gene in improving the broad-spectrum disease resistance of crops such as rice for the first time, and the UvWSC1 gene is subjected to heterologous high expression in the rice, so that the broad-spectrum resistance of the rice to various pathogenic bacteria such as Ustilaginoidea virens, Magnaporthe oryzae and Xanthomonas oryzae can be obviously enhanced, and the agronomic traits of the rice are not influenced; an important theoretical basis is provided for rice disease-resistant breeding. By heterologous expression of the UvWSC1 gene, broad-spectrum disease resistance can be safely and effectively endowed to crops, a broad-spectrum disease-resistant rice variety is obtained for production, and the broad-spectrum disease-resistant rice gene has great application prospects and economic values in the aspects of crop breeding disease resistance improvement, rice disease prevention and control and crop yield increase and pesticide reduction.

The invention discloses a rice bakanae disease bacterium-carrying seed detection method and device based on a hyperspectral image, and the method comprises the steps: preprocessing the hyperspectral data of rice seeds, and inputting the preprocessed data into a rice bakanae disease bacterium-carrying seed discrimination model; a hierarchical and adaptive feature learning and decision-making system is realized by using a multi-scale spectral feature extraction module, an adaptive spectral attention mechanism module, a deep feature cross fusion network module and a result discrimination module, the self-adaptive spectrum attention mechanism module dynamically analyzes the importance of each wave band, and the judgment result module adopts a parallel dynamic meta-learning classifier module and an uncertainty quantification module, and provides the credibility of a judgment result while outputting the judgment result of the seed health state. According to the method, efficient, accurate and lossless bacteria-carrying seed identification with quantifiable result reliability is realized.

The invention belongs to the technical field of gene engineering, and discloses application of an F-box protein gene OsFBX235 in improvement of bacterial blight resistance. The invention specifically discloses an application of knocking out OsFBX235 gene in improving the bacterial blight resistance of rice or cultivating a bacterial blight resistant rice variety. The F-box protein coding gene OsFBX235 is separated and cloned from rice, and the gene is proved to participate in the defensive reaction of the rice to the bacterial blight for the first time through functional analysis, and is an important negative regulation factor for regulating the resistance of the rice bacterial blight. The gene OsFBX235 is knocked out through target gene screening in combination with a CRISPR / Cas9 technology, and the importance of the gene OsFBX235 on resistance regulation and control of the rice bacterial blight is proved. Meanwhile, knockout of the gene can be used for creating rice germplasm resources with high bacterial leaf blight resistance and can be applied to breeding of new varieties with the bacterial leaf blight resistance of the rice.

The invention relates to a DNA (Deoxyribose Nucleic Acid) for regulating and controlling a resistance gene LTPL160 of rice bacterial blight and breeding utilization of a protein of the DNA. According to the invention, the gene OsLTPL160 for regulating and controlling the resistance of the rice bacterial leaf blight is cloned by virtue of a non-gel quantitative proteomics analysis and transcription analysis method. The OsLTPL160 knockout and overexpression material is obtained by adopting a gene editing and transgenic technology. The OsLTPL160 is knocked out, so that the bacterial blight resistance of the rice is remarkably improved; and overexpression of the OsLTPL160 obviously reduces the resistance of the bacterial blight of the rice. The cloning of the OsLTPL160 gene and the construction of the material provide material and technical support for the bacterial blight resistance of the rice.

The application discloses application of a rice disease resistance related gene Os04g55720 in bacterial blight breeding and relates to the technical field of modern biotechnology and genetic engineering technology.The rice disease resistance related gene Os04g55720 has a nucleotide sequence as shown in SEQ ID NO.1, is composed of 2821 nucleotides, has a coding region sequence as shown in SEQ ID NO.2, and has a sequence of 2148 nucleotides; the gene encodes a protein with 613 amino acids, and has an amino acid sequence as shown in SEQ ID NO.3.The application proves that overexpression of Os04g55720 enhances the resistance of rice to bacterial blight by means of molecular biology and biochemical technology, and shows that the rice protein OsPGDH-3 can effectively control the incidence of rice bacterial blight at a relatively low level.The application provides a new idea, strategy and gene resource for green prevention and control of rice bacterial blight.

The application discloses a SNP molecular marker for identifying or assisting in identifying rice bacterial leaf blight resistance and a primer composition thereof. The method comprises detecting polymorphism or genotype (allele) of an SNP site in a genome of a rice to be detected, and identifying or assisting in identifying rice bacterial leaf blight resistance according to the genotype, wherein the SNP site is a site on a chromosome 5 of the rice, and the nucleotide type is A or G, which is the 30th nucleotide in sequence 4 in a sequence table. The method can be used for predicting rice bacterial leaf blight resistance and rice breeding. The substance for detecting the polymorphism and genotype of the SNP site can be combined with other substances (such as a substance for detecting single nucleotide polymorphism or genotype of other molecular markers related to rice bacterial leaf blight resistance) to prepare a product for identifying rice bacterial leaf blight resistant varieties.

This invention relates to the field of medicinal chemistry, specifically to a capsaicin-like compound containing isopropanolamine, its preparation method, and its application. This type of compound has a structure as shown in general formula (I). The invention uses capsaicin and isopropanolamine as pharmacophores, connecting the two pharmacophores with different bridging chains to synthesize a series of capsaicin-like derivatives containing isopropanolamine. These compounds exhibit excellent inhibitory effects on plant pathogenic bacteria such as rice bacterial blight, citrus canker, rice bacterial leaf streak, and kiwifruit canker; and plant pathogenic fungi such as cucumber gray mold, pepper wilt, rapeseed sclerotinia rot, eggplant verticillium wilt, wheat scab, potato late blight, blueberry root rot, grape coccidioidomyces, dragon fruit anthracnose, and rice sheath blight, providing an important scientific basis for the research and development of new pesticides.

This invention discloses the application of the OsCAD3 protein and its encoding gene in regulating resistance to bacterial blight in rice, relating to the field of plant genetic engineering. The amino acid sequence of this gene protein is shown in SEQ ID NO.3. Cloning the gene editing site sequence, constructing a gene editing recombinant vector, and transforming it into rice can improve rice resistance to bacterial blight and ensure stable rice yield. The OsCAD3 gene editing vector constructed in this invention and the new OsCAD3 allele formed through genome editing can be applied to rice breeding for resistance to bacterial blight. Constructing an overexpression recombinant vector of the above gene coding region sequence and transforming it into rice can reduce rice resistance to bacterial blight and ensure stable rice yield. The above gene overexpression vector can be used in rice breeding to regulate bacterial blight. This invention can be used in the fields of rice germplasm resource improvement and genetic breeding, and is of great significance for breeding new rice varieties resistant to bacterial blight.

The invention discloses application of a rice ASG gene in regulation and control of rice bacterial leaf blight resistance, and belongs to the field of biological genetic breeding. According to the present invention, the new bacterial blight susceptible gene ASG (AvrXa23D-corresphindusceptilgene) in the rice ZH11 is screened and identified, and the fact that the resistance of the rice to the bacterial blight containing the TAL effector AvrXa23D is enhanced after the gene is knocked out is further verified; therefore, the function of the ASG gene in the ZH11 is illustrated, the new application of the ASG gene in regulating and controlling the resistance of the rice bacterial blight is proved, and a brand new gene target and a breeding means are provided for solving the harm of the rice bacterial blight.

The application discloses a kind of rice bacterial leaf blight resistance related miRNA and belongs to crop disease-resistant variety breeding technical field, the application discloses the application of miR2871a-3p in the regulation of rice bacterial leaf blight resistance, the nucleotide sequence of miR2871a-3p is as shown in SEQ ID NO:1.The application is confirmed by experimental result, and the expression level of miR2871a-3p can significantly improve the resistance of rice to bacterial leaf blight, therefore, rice miR2871a-3p can be applied to the genetic engineering of crop, for cultivating disease-resistant rice variety.It can be seen that the miR2871a-3p provided in the application has good application prospect in the prevention and treatment of rice diseases, and lays an important foundation for rice disease-resistant breeding improvement.

The invention belongs to the technical field of biological prevention and control, and provides application of N-palmitoyl aspartic acid in improvement of resistance of rice bacterial blight, which is characterized in that N-palmitoyl aspartic acid is prepared into a resistance inducer for prevention and control of bacterial blight pathogen infection in a three-leaf one-core period of rice. The inhibition effect of the N-palmitoyl aspartic acid on the Xanthomonas oryzae pv. Oryzae is evaluated, and the result shows that the N-palmitoyl aspartic acid effectively reduces the gene expression of the Xanthomonas oryzae pv. Oryzae, inhibits the growth and propagation of the Xanthomonas oryzae pv. Oryzae, improves the resistance of rice, can replace or reduce the use of chemical bactericides, and is environment-friendly.

The present application discloses a kind of Magnaporthe grisea and Xanthomonas oryzae resistance related protein derived from rice and its coding gene and application.The present application provides a kind of method for improving the disease resistance of plant: the gene of plant coding OsAspRS protein is replaced by the gene of mutant protein.The present application also protects the application of osasprs mutant in the preparation of plant with improved disease resistance.osasprs mutant is a kind of spot-like mutant plant controlled by single recessive nuclear gene, which is screened by EMS on Huanghuazhan plant.Furthermore, the present application successfully locates and clones AspRS gene, and the mutation of the gene site can cause spot-like phenotype on rice leaf, and further improve the broad-spectrum disease resistance of rice.The present application is helpful to improve the disease resistance of crop, provides gene resources and technical support for cultivating new rice varieties with broad-spectrum resistance, and has important significance and application value for crop disease resistance breeding work.

The invention discloses a functional molecular marker of a bacterial blight resistance gene Xa7, a screening method and application of the functional molecular marker, and belongs to the technical field of molecular genetics. The inDel molecular marker of the bacterial leaf blight resistance gene Xa7 is Xa7-InDel, and the molecular marker of the bacterial leaf blight resistance gene Xa7 is Xa7-InDel; the Xa7-InDel is a molecular marker which is amplified from genome DNA (Deoxyribonucleic Acid) of a rice variety carrying the bacterial blight resistance gene Xa7 through a primer pair SEQ ID NO.1 and SEQ ID NO.2, and the molecular marker and the bacterial blight resistance gene Xa7 are in a specific banding pattern; the InDel molecular marker disclosed by the invention can be used for detecting bacterial leaf blight resistance genes in rice varieties, and can be simply, conveniently and quickly applied to rice disease-resistant breeding practice with high throughput, so that the disease-resistant breeding process is accelerated.

The invention discloses a biopesticide composition for preventing and treating sesame bacterial wilt and a preparation, and belongs to the technical field of plant protection. The biopesticide composition for preventing and treating sesame bacterial wilt comprises the following raw materials in parts by mass: 30-40 parts of 50% carbendazim wettable powder, 2-3 parts of spinach / chamomile mixed extract, 2.5-3.5 parts of citrus peel extract and 1.5-2 parts of compound microorganisms. The biopesticide composition for preventing and treating sesame bacterial wilt has a good bacteriostatic effect on pathogenic bacteria, can effectively inhibit infection of the pathogenic bacteria on plants, can reduce the dosage of chemical agents, can reduce the risk that the pathogenic bacteria generate drug resistance, can effectively prevent and relieve the disease condition of sesame, and has a good application prospect. The influence of bacterial wilt on the economic crop yield is reduced.

This divisional application discloses the use of a 2,8-bis(trifluoromethyl)-4-hydroxyquinoline derivative in the control or treatment of agricultural pathogenic bacteria. The structural formula of the 2,8-bis(trifluoromethyl)-4-hydroxyquinoline derivative is as follows: Bioactivity tests show that this derivative exhibits excellent inhibitory activity against two plant bacteria: rice bacterial blight pathogen and citrus canker pathogen. Compound Q-29 shows an MIC90 value of 1.56 μg / mL against both rice bacterial blight pathogen and citrus canker pathogen, significantly outperforming the control drugs thiabendazole and thiabendazole copper. The compounds involved in this invention are easy to prepare, use inexpensive and readily available raw materials, and are expected to be developed into a novel agricultural antibacterial agent.

The invention discloses a probe method real-time fluorescent PCR (polymerase chain reaction) rapid detection primer and probe combination for cruciferae bacterial phytophthora blight and a detection method, and belongs to the technical field of biological detection. According to the probe method real-time fluorescent PCR rapid detection method for the cruciferae bacterial phytophthora blight, DNA of a tested strain is used as a template, and the probe method real-time fluorescent PCR rapid detection is carried out by using a primer and probe combination. The detection method has the advantages of high specificity, high accuracy, high sensitivity, rapidness, high efficiency, quantitative analysis, simple operation, good repeatability and strong applicability.

This invention belongs to the field of microbial technology, specifically relating to a type of endophytic Bacillus belye AN6 in rice and its applications. Specifically, this invention provides a Bacillus belye AN6, classified and named as follows: Bacillus velezensis This invention provides a novel rice endophytic Bacillus bereaves strain AN6. Through isolation, screening, identification, and experimental verification, it was found that this strain exhibits broad-spectrum resistance to various plant pathogens, including rice bacterial blight and rice blast. This strain has the ability to inhibit multiple plant pathogens and adsorb heavy metals, which is of great significance for the biological control of rice diseases and the development of green agriculture. (CCTCC accession number: M 20231886)

This invention belongs to the field of plant genetic engineering technology, specifically relating to a method for improving the disease resistance of rice. SCRE10 Genes and their applications, the aforementioned SCRE10 The base sequence of the gene is shown in SEQ ID NO.1, and the amino acid sequence is shown in SEQ ID NO.2. This invention utilizes a dexamethasone-induced expression mechanism. SCRE10 The transgenic rice plants were found to significantly induce... PR Gene expression and reactive oxygen species surges, enhancing rice resistance to rice false smut and bacterial blight. This invention demonstrates heterologous induction of rice false smut pathogen expression. SCRE10 Genes have the function of positively regulating the disease resistance of rice. SCRE10 Genes can be used to improve the disease resistance of rice, which is of great significance for the creation of disease-resistant rice germplasm.

The application discloses application of OsCPK8 gene in regulation of rice disease resistance and belongs to the technical field of molecular biology. The DNA sequence of the CPK8 gene is shown as SEQ ID NO. 1, and the coding region CDS sequence thereof is shown as SEQ ID NO. 2. The application mines and applies the rice CPK8 gene, and it is clear that the knockout mutant of the rice CPK8 gene significantly enhances the resistance of rice to rice blast and bacterial blight, and the active oxygen burst induced by chitin and flg22 is significantly enhanced. The gene function loss can significantly improve the rice resistance, indicating that the gene negatively regulates the immunity of rice, and the gene can be used as a target gene for improving the resistance of rice to rice blast and bacterial blight. The application provides a new and efficient target for rice molecular breeding, and has important significance for guaranteeing safe production of rice and promoting development of green agriculture.

The invention belongs to the technical field of gene engineering, and particularly relates to application of a biological product related to an OsRIEL gene mutant in rice disease resistance. The nucleotide sequence of the OsRIEL gene mutant is as shown in SEQ ID NO. 1 or SEQ ID NO. 2; the rice disease resistance refers to rice blast resistance and bacterial leaf blight resistance; the gene OsRIEL is knocked out through a CRISPR / Cas9 technology, and a knockout mutant osriel is obtained; it is found through rice blast resistance identification that compared with wild type rice, the mutant osriel is more resistant to diseases and small in disease spot area, the number of magnaporthe oryzae carried by diseased leaves is small, and it is indicated that OsRIEL negatively regulates the resistance of rice to rice blast; the bacterial leaf blight resistance identification finds that compared with wild rice, the mutant osriel is more resistant to diseases and shorter in disease spots, which indicates that the OsRIEL negatively regulates the resistance of the rice to the bacterial leaf blight.

The invention discloses an application of an OsExo70FX9a gene and an encoding protein thereof in improving bacterial blight resistance of rice, and belongs to the technical field of biology. The nucleotide sequence of the OsExo70FX9a gene is as shown in SEQ ID NO. 1 (sequence identifier number 1). The invention finds that the OsExo70FX9a gene can be obviously expressed when being induced by Xanthomonas oryzae pv. Oryzae, the resistance of rice to the Xanthomonas oryzae pv. Oryzae can be improved by improving the expression of the OsExo70FX9a gene, an overexpression strain of the OsExo70FX9a gene of the rice is constructed, the overexpression strain has broad-spectrum resistance to the Xanthomonas oryzae pv. Oryzae, and by optimizing a promoter, the expression of the OsExo70FX9a gene can be improved. According to the invention, the OsExo70FX9a overexpression strain successfully realizes that the seedling length, the root length and the grain size in the seedling stage are only slightly influenced while the bacterial blight resistance is effectively improved, a basis is provided for rice bacterial blight resistance breeding, and the OsExo70FX9a overexpression strain has a wide application prospect.

The invention discloses an application of any compound of an Oxoapophine alkaloid derivative in prevention and treatment of agricultural diseases. A biological activity test shows that the derivative shows certain inhibitory activity on two plant pathogenic bacteria such as rice bacterial blight and citrus canker pathogen, and five plant pathogenic fungi such as rhizoctonia solani, sclerotinia sclerotiorum, botrytis cinerea, fusarium graminearum, magnaporthe grisea and wheat stem rot; the compound has a good inhibition effect on citrus canker pathogen and sclerotinia sclerotiorum. The compound is low in preparation difficulty, raw materials are cheap and easy to obtain, and the compound is expected to be developed into a novel agricultural antibacterial agent.

This invention belongs to the field of gene breeding technology, specifically relating to the application of the cascade signal kinase OsMAPKKK5 in improving rice resistance to bacterial blight. This invention provides the protein OsMAPKKK5 or substances regulating the activity and / or content of said protein, and the application of related biological materials. This invention constructs... OsMAPKKK5 Transgenic lines overexpressing the pathogen were inoculated with bacterial blight pathogens in the field for resistance phenotypic analysis, and the results showed that... OsMAPKKK5 Compared to wild-type rice varieties, the overexpressed transgenic lines showed shorter lesions and exhibited a resistance phenotype against bacterial blight, thus demonstrating improved immunity. OsMAPKKK5 The expression level of this gene can promote the resistance to bacterial blight in rice. This invention provides important genetic resources and molecular theoretical basis for the development of new rice varieties resistant to bacterial blight.

The application discloses a strain of Pseudomonas extremorientalis LJ-6 and application of the strain in preventing and controlling mulberry diseases. The Pseudomonas extremorientalis in the application is preserved in the Guangdong Microbial Culture Collection Center on June 18, 2024, and the preservation number is GDMCC NO: 64766. The Pseudomonas extremorientalis LJ-6 has good antagonistic effects on bacterial pathogenic bacteria such as Caulobacter segnis, Pantoea ananatis, Klebsiella michiganensis and Enterobacter cloacae, and fungal pathogenic bacteria such as Fusarium oxysporum, Curvularia lunata and Guignardia bidwellii. The Pseudomonas extremorientalis LJ-6 has good prevention and control effects on plant diseases caused by the above pathogenic bacteria invading plants, such as mulberry bacterial wilt, mulberry bacterial blight, mulberry leaf spot and mulberry anthracnose. Therefore, the Pseudomonas extremorientalis LJ-6 has good application prospect and value in preventing and controlling plant diseases such as mulberry and preparing disease prevention and control preparation products.

The application discloses a bacteriophage E12-2 of Xanthomonas oryzae and application thereof, and belongs to the technical field of microorganisms. The bacteriophage is obtained by using Xanthomonas oryzae with bacteriophage resistance for multiple rounds of directional evolution, is named Xanthomonas oryzae phage E12-2, and is preserved in the China Center for Type Culture Collection on March 17, 2025, has a preservation address of No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province, and a preservation number of CCTCC NO: M 2025486. The bacteriophage can lyse common Xanthomonas oryzae and resistant strains thereof, has a wide host spectrum, strong lytic activity, and a wide application prospect in the prevention and treatment of Xanthomonas oryzae diseases.

The application discloses a SNP molecular marker for detecting rice bacterial blight resistance gene Xa23 and application thereof, and the SNP molecular marker is one of K_110532, K_110534, K_110536, K_110543 and K_110547. The SNP molecular marker screened by the scheme has good stability, is not influenced by other gene effects and environmental factors, can be selected in an early generation, shortens a breeding period, improves breeding efficiency, has important significance for rice bacterial blight resistance variety improvement, is suitable for assisted selection breeding of the Xa23 gene, and is suitable for popularization and application.

The application belongs to the technical field of genetic engineering, and discloses an F-box protein gene OsFBX235 application in improving white leaf blight resistance. The application specifically discloses a knockout OsFBX235 application of the gene in improving rice white leaf blight resistance or cultivating rice varieties resistant to white leaf blight. The application separates and clones an F-box protein coding gene OsFBX235 from rice, and through functional analysis, it is first confirmed that the gene is involved in the defense reaction of rice to white leaf blight bacteria and is an important negative regulatory factor for regulating rice white leaf blight resistance. Through target gene screening and CRISPR / Cas9 technology, the gene is knocked out OsFBX235 , and the importance of the gene to the regulation of rice white leaf blight resistance is proved OsFBX235 . Meanwhile, the knockout of the gene can be used for creating rice germplasm resources with high white leaf blight resistance, and can be applied to the breeding of new rice varieties resistant to white leaf blight.

The invention discloses a preparation method of a pyrrolidone derivative, which comprises the following steps: taking a carbonyl compound as a raw material, reacting with ammonium acetate in an ethanol solution to obtain an amino compound 2, reacting the amino compound 2 under the action of oxalyl chloride to obtain a dicarbonyl pyrrole compound 4, and reacting the dicarbonyl pyrrole compound 4 with a Witting reagent to obtain a pyrrolidone derivative 5. According to the invention, a series of novel pyrrole-3-ketone compounds are prepared from a carbonyl compound, the synthesized pyrrole-3-ketone derivatives are not reported, and a part of the compounds have high bacteriostatic activity and are applied to inhibition of kiwifruit canker pathogen and rice bacterial blight pathogen.