117 results about "Bacterial blight" patented technology

The invention provides a Ustilaginoidea virens gene UvASE2 for regulating and controlling rice resistance and application of the Ustilaginoidea virens gene UvASE2, and relates to the technical field of plant genetic engineering. The nucleotide sequence of the Ustilaginoidea virens gene UvASE2 is as shown in SEQ ID NO: 1, and the amino acid sequence of the encoding protein of the Ustilaginoidea virens gene UvASE2 is as shown in SEQ ID NO: 2. By constructing a transgenic rice plant for heterologous expression of UvASE2, it is found that the transgenic rice can significantly improve the resistance to false smut and bacterial blight and significantly induce the outbreak of active oxygen. The heterologous expression UvASE2 gene of Ustilaginoidea virens has the function of positively regulating and controlling the disease resistance of rice, and the UvASE2 gene can be used for improving the disease resistance of rice and has important significance on creation of disease-resistant germplasm of rice.

The invention discloses pseudomonas chlororaphis S7-24 for antagonizing xanthomonas oryzae and application of the pseudomonas chlororaphis S7-24, and belongs to the technical field of microorganisms. The pseudomonas chlororaphis S7-24 is preserved in the China Center for Type Culture Collection on November 12, 2025, and the preservation number of the pseudomonas chlororaphis S7-24 is CCTCC NO: M 20252533. The pseudomonas chlororaphis S7-24 provided by the invention is a newly found pseudomonas biocontrol resource, and verification shows that the strain S7-24 has relatively high antagonistic activity on rice bacterial blight and rice bacterial streak, and can be used for effectively preventing and treating rice bacterial blight and rice bacterial streak. The pseudomonas chlororaphis S7-24 provided by the invention is used for preventing and treating rice bacterial leaf blight and rice bacterial leaf streak, can reduce the use amount of chemical pesticides, ensures grain safety, and has better economic and ecological benefits.

The invention belongs to the technical field of biological prevention and control, and particularly relates to a Burkholderia paragallinarum D15 and application of the Burkholderia paragallinarum D15. The Burkholderia parakholderia sp. D15 is preserved in China General Microbiological Culture Collection Center (CGMCC) on November 25, 2024, the address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO. 1.61962. The Burkholderia parakholderia sp. D15 has the advantages that the Burkholderia parakholderia sp. According to the invention, a multifunctional strain Burkholderia paragallinarum D15 is separated from the rock wall of Danxia red rock, and the Burkholderia paragallinarum D15 has a relatively good antagonistic effect on most plant pathogens and has a relatively strong bacteriostatic effect on xanthomonas oryzae Xoo causing rice bacterial blight; the compound also has a relatively strong bacteriostatic effect on other pathogenic bacteria causing crop diseases, such as rice sheath blight disease, rice blast, fusarium graminearum, sclerotium rolfsii and ralstonia solanacearum, and lays a foundation for prevention and treatment of rice bacterial blight and development of related biological preparations.

The invention belongs to the field of molecular biology, and particularly relates to application of OsDOF3 protein or a coding gene thereof to improvement of rice broad-spectrum disease resistance and regulation of agronomic traits. According to the application, an OsDOF3 knockout mutant and an overexpression transgenic line are constructed under the background of susceptible rice, resistance phenotype analysis is performed by inoculating various pathogenic bacteria (including pyricularia oryzae, rhizoctonia solani and bacterial blight), and it is proved that OsDOF3 participates in regulation and control of an immunoreaction pathway of rice to fungal diseases (rice blast and sheath blight) and bacterial diseases (bacterial blight). Meanwhile, the application also finds that after a strong promoter ubi is adopted for driving, an overexpression material of the OsDOF3 is shorter than that of a wild plant, and the OsDOF3 influences normal growth and development of rice while stimulating plant immune response. The invention provides important gene resources and molecular theoretical basis for developing a new variety of broad-spectrum disease-resistant rice.

The invention relates to the technical field of agricultural protection, in particular to an amide thiazole compound as well as a preparation method and application thereof. The amide nitrothiazole compound shown in the formula (1) is provided by carrying out proper structural modification on nitazoxanide, and the compound has good biological activity, can be applied to prevention and treatment of cryptosporidiosis and has a certain inhibition effect on florae such as rice streak, rice bacterial leaf blight, rice ralstonia solanacearum, cabbage soft rot and citrus ulcer. Formula (1): # imgabs0 #

The invention relates to the technical field of rice disease-resistant breeding, in particular to a rice blast and bacterial leaf blight resistant related gene OsRLK3 of rice and application of the rice blast and bacterial leaf blight resistant related gene OsRLK3. The CDS sequence of the OsRLK3 gene is 3885 bp, and 1294 amino acids are encoded; the invention discovers and proves that the OsRLK3 gene positively regulates the resistance of rice to rice blast and bacterial leaf blight for the first time, the resistance of rice to rice blast and bacterial leaf blight can be reduced by knocking out the OsRLK3 gene through a CRISPR / Cas9 method, and the resistance of rice to rice blast and bacterial leaf blight can be remarkably enhanced by improving the expression level of the OsRLK3 gene; in addition, knockout and overexpression of the OsRLK3 gene do not affect normal growth and development of rice. Therefore, the invention can be applied to genetic improvement for improving resistance and yield of related agricultural crops such as rice and the like.

The invention relates to the technical field of agricultural disease detection and crop disease resistance screening, in particular to a rice bacterial leaf blight disease resistance screening method based on an unmanned aerial vehicle and deep learning, and adopts the technical scheme that an optimized YOLOv11-OBB model is composed of a C3k2FC module, an SPPFLSKA module, a SlimNeck module and a LiteHead module, is responsible for extracting features in an image and positioning bacterial leaf blight spots, and is used for screening the disease resistance of the rice bacterial leaf blight; bacterial leaf blight can be efficiently and accurately detected; a lightweight deep learning model is used, efficient operation on a resource-limited unmanned aerial vehicle platform can be realized, and the equipment and calculation cost is reduced; through the cooperation of a plurality of optimization modules, the detection precision of bacterial leaf blight spots is remarkably improved, and a stable detection effect can be kept; the operation process is simplified, and full automation of bacterial blight disease resistance screening is realized; therefore, low-cost, efficient, automatic and high-precision bacterial blight disease resistance screening is realized by utilizing the lightweight and optimized YOLOv11-OBB model and combining module design with high calculation efficiency, and the method is suitable for large-scale field application.

The invention provides an application of a Ustilaginoidea virens UvWSC1 gene in improvement of plant disease resistance. The invention develops a new application of the Ustilaginoidea virens UvWSC1 gene in improving the broad-spectrum disease resistance of crops such as rice for the first time, and the UvWSC1 gene is subjected to heterologous high expression in the rice, so that the broad-spectrum resistance of the rice to various pathogenic bacteria such as Ustilaginoidea virens, Magnaporthe oryzae and Xanthomonas oryzae can be obviously enhanced, and the agronomic traits of the rice are not influenced; an important theoretical basis is provided for rice disease-resistant breeding. By heterologous expression of the UvWSC1 gene, broad-spectrum disease resistance can be safely and effectively endowed to crops, a broad-spectrum disease-resistant rice variety is obtained for production, and the broad-spectrum disease-resistant rice gene has great application prospects and economic values in the aspects of crop breeding disease resistance improvement, rice disease prevention and control and crop yield increase and pesticide reduction.

The invention belongs to the technical field of plant genetic engineering, and discloses a gene OSD4 participating in a synthetic route of PAL-dependent salicylic acid (SA) of rice and application of the gene OSD4. A mutant osd4 is obtained by knocking out OSD4 through CRISPR / Cas9, the SA content of leaves of the mutant osd4 is reduced to 15% of that of a wild type, and the mutant osd4 is more sensitive to xanthomonas oryzae pv. And constructing an OSD4 overexpression vector to obtain a transgenic material with enhanced resistance. Hormone determination and inoculation experiments show that OSD4 regulates SA synthesis and participates in disease-resistant reaction. The in-vitro enzyme activity is verified by constructing an OSD4-pMAL carrier, and it is proved that OSD4 can catalyze BS to directly generate SA. The gene can be used for disease resistance improvement of crops such as rice.

The invention discloses a rice bakanae disease bacterium-carrying seed detection method and device based on a hyperspectral image, and the method comprises the steps: preprocessing the hyperspectral data of rice seeds, and inputting the preprocessed data into a rice bakanae disease bacterium-carrying seed discrimination model; a hierarchical and adaptive feature learning and decision-making system is realized by using a multi-scale spectral feature extraction module, an adaptive spectral attention mechanism module, a deep feature cross fusion network module and a result discrimination module, the self-adaptive spectrum attention mechanism module dynamically analyzes the importance of each wave band, and the judgment result module adopts a parallel dynamic meta-learning classifier module and an uncertainty quantification module, and provides the credibility of a judgment result while outputting the judgment result of the seed health state. According to the method, efficient, accurate and lossless bacteria-carrying seed identification with quantifiable result reliability is realized.

The invention belongs to the technical field of gene engineering, and discloses application of an F-box protein gene OsFBX235 in improvement of bacterial blight resistance. The invention specifically discloses an application of knocking out OsFBX235 gene in improving the bacterial blight resistance of rice or cultivating a bacterial blight resistant rice variety. The F-box protein coding gene OsFBX235 is separated and cloned from rice, and the gene is proved to participate in the defensive reaction of the rice to the bacterial blight for the first time through functional analysis, and is an important negative regulation factor for regulating the resistance of the rice bacterial blight. The gene OsFBX235 is knocked out through target gene screening in combination with a CRISPR / Cas9 technology, and the importance of the gene OsFBX235 on resistance regulation and control of the rice bacterial blight is proved. Meanwhile, knockout of the gene can be used for creating rice germplasm resources with high bacterial leaf blight resistance and can be applied to breeding of new varieties with the bacterial leaf blight resistance of the rice.

The invention relates to a DNA (Deoxyribose Nucleic Acid) for regulating and controlling a resistance gene LTPL160 of rice bacterial blight and breeding utilization of a protein of the DNA. According to the invention, the gene OsLTPL160 for regulating and controlling the resistance of the rice bacterial leaf blight is cloned by virtue of a non-gel quantitative proteomics analysis and transcription analysis method. The OsLTPL160 knockout and overexpression material is obtained by adopting a gene editing and transgenic technology. The OsLTPL160 is knocked out, so that the bacterial blight resistance of the rice is remarkably improved; and overexpression of the OsLTPL160 obviously reduces the resistance of the bacterial blight of the rice. The cloning of the OsLTPL160 gene and the construction of the material provide material and technical support for the bacterial blight resistance of the rice.

The application discloses application of a rice disease resistance related gene Os04g55720 in bacterial blight breeding and relates to the technical field of modern biotechnology and genetic engineering technology.The rice disease resistance related gene Os04g55720 has a nucleotide sequence as shown in SEQ ID NO.1, is composed of 2821 nucleotides, has a coding region sequence as shown in SEQ ID NO.2, and has a sequence of 2148 nucleotides; the gene encodes a protein with 613 amino acids, and has an amino acid sequence as shown in SEQ ID NO.3.The application proves that overexpression of Os04g55720 enhances the resistance of rice to bacterial blight by means of molecular biology and biochemical technology, and shows that the rice protein OsPGDH-3 can effectively control the incidence of rice bacterial blight at a relatively low level.The application provides a new idea, strategy and gene resource for green prevention and control of rice bacterial blight.

The invention relates to the technical field of biology, in particular to application of rice LOCOs06g20960 protein to regulation and control of plant disease resistance. The invention discloses application of a substance for protecting the protein or regulating the expression substance of the coding gene of the protein or regulating the activity or content of the protein in any one of Y1-Y5: application of Y1 in regulating the disease resistance of plants; the Y2 is applied to preparation of a product for regulating and controlling plant disease resistance; the Y3 is applied to cultivation of plants with changed disease resistance; the Y4 is applied to preparation of products for cultivating plants with changed disease resistance; the invention discloses application of Y5 in plant breeding. It is found that the rice LOCOs06g20960 protein has the function of positively regulating and controlling the bacterial blight resistance of rice, the bacterial blight resistance of the rice can be remarkably improved by transferring the LOCOs06g20960 gene overexpression sequence into the rice, and the rice LOCOs06g20960 gene overexpression sequence is of great significance to cultivation of new bacterial blight resistant rice varieties.

The application discloses a SNP molecular marker for identifying or assisting in identifying rice bacterial leaf blight resistance and a primer composition thereof. The method comprises detecting polymorphism or genotype (allele) of an SNP site in a genome of a rice to be detected, and identifying or assisting in identifying rice bacterial leaf blight resistance according to the genotype, wherein the SNP site is a site on a chromosome 5 of the rice, and the nucleotide type is A or G, which is the 30th nucleotide in sequence 4 in a sequence table. The method can be used for predicting rice bacterial leaf blight resistance and rice breeding. The substance for detecting the polymorphism and genotype of the SNP site can be combined with other substances (such as a substance for detecting single nucleotide polymorphism or genotype of other molecular markers related to rice bacterial leaf blight resistance) to prepare a product for identifying rice bacterial leaf blight resistant varieties.

The invention discloses application of an ELSA gene to improvement of rice disease resistance. Researches show that knockout of an ELSA gene promoter region or a gene body region can improve the resistance of rice to bacterial leaf blight, bacterial streak and rice blast, has no significant influence on the plant type, panicle type and the like of rice, and maintains the stability of the rice yield, that is, the rice yield is not influenced. It is indicated that the ELSA gene is a negative regulation gene of rice bacterial leaf blight, bacterial streak and rice blast resistance. Therefore, the rice with high disease resistance and stable yield can be cultivated by knocking out the rice ELSA gene promoter region or gene body region, and a simple and effective method is provided for cultivating the rice with high disease resistance and stable yield.

The invention discloses application of pseudomonas aeruginosa T17 in prevention and treatment of rice bacterial blight. The invention discloses pseudomonas aeruginosa T17 and a function of a fermentation broth thereof as a biological agent in the aspect of disease control. Experiments prove that the pseudomonas aeruginosa T17 can directly inhibit growth of bacterial blight pathogenic bacteria (Xoo) and pyricularia oryzae (M.oryzae). Meanwhile, the fermentation product filtrate can directly induce up-regulated expression of rice defense related genes and up-regulated expression of jasmonic acid (JA) synthesis key genes OsAOS2, and the resistance of rice to bacterial blight can be enhanced by pretreating rice with the fermentation product filtrate. Therefore, the research lays a foundation for taking the pseudomonas aeruginosa T17 and the fermented filtrate thereof as the rice immune resistance inducer, and provides a new thought for developing innovative pollution-free botanical pesticides.

This invention relates to the field of medicinal chemistry, specifically to a capsaicin-like compound containing isopropanolamine, its preparation method, and its application. This type of compound has a structure as shown in general formula (I). The invention uses capsaicin and isopropanolamine as pharmacophores, connecting the two pharmacophores with different bridging chains to synthesize a series of capsaicin-like derivatives containing isopropanolamine. These compounds exhibit excellent inhibitory effects on plant pathogenic bacteria such as rice bacterial blight, citrus canker, rice bacterial leaf streak, and kiwifruit canker; and plant pathogenic fungi such as cucumber gray mold, pepper wilt, rapeseed sclerotinia rot, eggplant verticillium wilt, wheat scab, potato late blight, blueberry root rot, grape coccidioidomyces, dragon fruit anthracnose, and rice sheath blight, providing an important scientific basis for the research and development of new pesticides.

The invention belongs to the technical field of gene engineering, and particularly relates to application of a rice OsLBD gene to improvement of bacterial blight resistance of rice, and the nucleotide sequence of the OsLBD gene is shown as SEQ ID No.1. According to the invention, a regulation mechanism of rice disease resistance is disclosed from a molecular level, a new related gene for regulating disease resistance is found, and the gene is applied to inhibition of rice bacterial leaf blight, so that a foundation is laid for cultivation of rice bacterial leaf blight resistant varieties.

The present invention discloses a chalcone derivative containing a benzoxazole structure, a preparation method thereof, and an application thereof, and belongs to the field of chemical technology. The present invention uses 1-(4-(3-bromopropyloxy)phenyl)-3-(substituted aromatic)-2-propylene-1-one or 1-(4-(4-bromobutyloxy)phenyl)-3-(substituted aromatic)-2-propylene-1-one to undergo an etherification reaction with benzo[d]oxazole-2-thiol to generate a chalcone derivative containing a benzoxazole structure, the structural formula of which is as follows, wherein n is 3 or 4, and R is any one of a phenyl, a substituted phenyl, a thienyl, or a furyl group. Experimental testing has shown that the derivative has good inhibitory activity against plant bacteria such as rice bacterial blight, melon wilt, and potato soft rot, and can be used as a potential agent for inhibiting plant bacteria.

The invention discloses application of a sorghum SbPGK1 gene in improving plant disease resistance, and belongs to the technical field of biology. The nucleotide sequence of the sorghum SbPGK1 gene is as shown in SEQ ID NO.9. By analyzing phenotypes and related physiological indexes of SbPGK1 rice transgenic lines before and after inoculation of different pathogenic bacteria for infection and sorghum, the results show that compared with non-SbPGK1 gene rice lines, the SbPGK1 transgenic lines have obviously better resistance to magnaporthe oryzae and bacterial blight germs than wild types, silence SbPGK1 in sorghum has better resistance to rice blast germs and bacterial blight germs, and the SbPGK1 gene has better resistance to rice blast germs and bacterial blight germs. The SbPGK1 gene has the advantages that the SbPGK1 silent strain has reduced disease resistance to colletotrichum gloeosporioides, the SbPGK1 is overexpressed in sorghum, and the SbPGK1 overexpressed strain has enhanced disease resistance to colletotrichum gloeosporioides, so that the SbPGK1 gene is a potential disease-resistant breeding gene and can be used for plant disease-resistant genetic improvement.

This invention discloses the application of the OsCAD3 protein and its encoding gene in regulating resistance to bacterial blight in rice, relating to the field of plant genetic engineering. The amino acid sequence of this gene protein is shown in SEQ ID NO.3. Cloning the gene editing site sequence, constructing a gene editing recombinant vector, and transforming it into rice can improve rice resistance to bacterial blight and ensure stable rice yield. The OsCAD3 gene editing vector constructed in this invention and the new OsCAD3 allele formed through genome editing can be applied to rice breeding for resistance to bacterial blight. Constructing an overexpression recombinant vector of the above gene coding region sequence and transforming it into rice can reduce rice resistance to bacterial blight and ensure stable rice yield. The above gene overexpression vector can be used in rice breeding to regulate bacterial blight. This invention can be used in the fields of rice germplasm resource improvement and genetic breeding, and is of great significance for breeding new rice varieties resistant to bacterial blight.

The invention discloses application of a rice ASG gene in regulation and control of rice bacterial leaf blight resistance, and belongs to the field of biological genetic breeding. According to the present invention, the new bacterial blight susceptible gene ASG (AvrXa23D-corresphindusceptilgene) in the rice ZH11 is screened and identified, and the fact that the resistance of the rice to the bacterial blight containing the TAL effector AvrXa23D is enhanced after the gene is knocked out is further verified; therefore, the function of the ASG gene in the ZH11 is illustrated, the new application of the ASG gene in regulating and controlling the resistance of the rice bacterial blight is proved, and a brand new gene target and a breeding means are provided for solving the harm of the rice bacterial blight.

The application discloses a kind of rice bacterial leaf blight resistance related miRNA and belongs to crop disease-resistant variety breeding technical field, the application discloses the application of miR2871a-3p in the regulation of rice bacterial leaf blight resistance, the nucleotide sequence of miR2871a-3p is as shown in SEQ ID NO:1.The application is confirmed by experimental result, and the expression level of miR2871a-3p can significantly improve the resistance of rice to bacterial leaf blight, therefore, rice miR2871a-3p can be applied to the genetic engineering of crop, for cultivating disease-resistant rice variety.It can be seen that the miR2871a-3p provided in the application has good application prospect in the prevention and treatment of rice diseases, and lays an important foundation for rice disease-resistant breeding improvement.

The invention discloses a lyase preparation for preventing and treating rice bacterial blight and a preparation method and application thereof, and relates to the technical field of agricultural biology.The lyase preparation comprises one or more of lyase LysXO1 of xanthomonas phage GRNXOP1, lyase LysXO5 of xanthomonas phage GRNXOP5 and lyase LysXO6 of xanthomonas phage GRNXOP6; wherein the amino acid sequence of the LysXO1 is as shown in SEQ ID NO. 1; the amino acid sequence of the LysXO5 is as shown in SEQ ID NO. 2; and the amino acid sequence of the LysXO6 is as shown in SEQ ID NO. 3. The lyase preparation provided by the invention has the advantages of being efficient in sterilization, safe to use, environment-friendly and the like, and a novel effective solution is provided for green prevention and control of rice bacterial blight.

The invention belongs to the technical field of biological prevention and control, and provides application of N-palmitoyl aspartic acid in improvement of resistance of rice bacterial blight, which is characterized in that N-palmitoyl aspartic acid is prepared into a resistance inducer for prevention and control of bacterial blight pathogen infection in a three-leaf one-core period of rice. The inhibition effect of the N-palmitoyl aspartic acid on the Xanthomonas oryzae pv. Oryzae is evaluated, and the result shows that the N-palmitoyl aspartic acid effectively reduces the gene expression of the Xanthomonas oryzae pv. Oryzae, inhibits the growth and propagation of the Xanthomonas oryzae pv. Oryzae, improves the resistance of rice, can replace or reduce the use of chemical bactericides, and is environment-friendly.

The present invention discloses a rice SnRK1β subunit protein related to plant disease resistance, its coding gene and applications. The SnRK1β protein provided by the present invention is the protein shown in Sequence 2, 4 or 6, and the coding gene of the SnRK1β subunit protein is the DNA molecule shown in Sequence 1, 3 or 5. The knockout of the SnRK1β subunit enhances the resistance to rice blast and bacterial blight. After treatment with pathogen-associated molecular pattern (Chitin), it leads to the up-regulated expression of pathogenesis-related proteins, the accumulation of ROS levels, and the activation of the MAPK pathway. In addition, the deletion of the SnRK1β1A gene does not affect the main agronomic traits of rice, indicating that the SnRK1β gene has good application potential in enhancing crop disease resistance. The present invention uses genetic engineering means to regulate the expression level of the SnRK1β gene for molecular breeding, which has potential application value in improving crop disease resistance.

The invention relates to pseudomonas in northwest province and application thereof in prevention and treatment of bacterial blight of rice, the strain is preserved in China General Microbiological Culture Collection Center, the address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation number is CGMCC NO.25493, the preservation date is August 5, 2022, and the preservation number is CGMCC NO.25493. The strain can be used for preventing and treating bacterial leaf blight caused by xanthomonas oryzae pv. Oryzae pv. Oryzae, the effect is remarkable, the prevention effect in a pot experiment reaches 72%, meanwhile, the disease time can be delayed, and therefore the strain has the huge potential of being applied as a biopesticide.

The present application discloses a kind of Magnaporthe grisea and Xanthomonas oryzae resistance related protein derived from rice and its coding gene and application.The present application provides a kind of method for improving the disease resistance of plant: the gene of plant coding OsAspRS protein is replaced by the gene of mutant protein.The present application also protects the application of osasprs mutant in the preparation of plant with improved disease resistance.osasprs mutant is a kind of spot-like mutant plant controlled by single recessive nuclear gene, which is screened by EMS on Huanghuazhan plant.Furthermore, the present application successfully locates and clones AspRS gene, and the mutation of the gene site can cause spot-like phenotype on rice leaf, and further improve the broad-spectrum disease resistance of rice.The present application is helpful to improve the disease resistance of crop, provides gene resources and technical support for cultivating new rice varieties with broad-spectrum resistance, and has important significance and application value for crop disease resistance breeding work.

The invention discloses a functional molecular marker of a bacterial blight resistance gene Xa7, a screening method and application of the functional molecular marker, and belongs to the technical field of molecular genetics. The inDel molecular marker of the bacterial leaf blight resistance gene Xa7 is Xa7-InDel, and the molecular marker of the bacterial leaf blight resistance gene Xa7 is Xa7-InDel; the Xa7-InDel is a molecular marker which is amplified from genome DNA (Deoxyribonucleic Acid) of a rice variety carrying the bacterial blight resistance gene Xa7 through a primer pair SEQ ID NO.1 and SEQ ID NO.2, and the molecular marker and the bacterial blight resistance gene Xa7 are in a specific banding pattern; the InDel molecular marker disclosed by the invention can be used for detecting bacterial leaf blight resistance genes in rice varieties, and can be simply, conveniently and quickly applied to rice disease-resistant breeding practice with high throughput, so that the disease-resistant breeding process is accelerated.