131 results about "Ethyl bromoacetate" patented technology

The invention discloses a quinazolinone compound containing 1, 2, 4-triazole thioether and synthesizing method and application of quinazolinone compound. The quinazolinone compound for preventing and curing plant pathogens is indicated by the general formula (I). Methyl anthranilate, formamide, ethyl bromoacetate, hydrazine hydrate, phenyl isothiocyanate, substitution benzyl halide or 2-chlorine-5 chloromethyl pyridine is used as raw material and subjected to six-step reaction to synthesize the target compound. The quinazolinone compounds A7, A8 and A19 are good in inhibitory activity on rice bacterial leaf blight germs. The quinazolinone compound A19 is good in inhibitory activity on citrus canker germs. The inhibitory activity on wheat scab germs of the quinazolinone compounds A14 and A18 and the inhibitory activity on apple rotting germs of the quinazolinone compound A8 are equal to that of control chemical hymexazol.

The invention provides a hybridoma cell strain MOP1.0 capable of generating an anti-morphine monoclonal antibody, wherein the cell strain is preserved in China Center for Type Culture Collection with a preservation number of CCTCCNO. C2013159. The invention also provides the anti-morphine monoclonal antibody secreted by the hybridoma cell strain MOP1.0. The invention also provides a kit for detecting morphine in saliva. The invention further provides a detection method and a manufacturing method of the morphine detection kit. The hybridoma cell strain MOP1.0 has the benefit that the artificial antigen of obtained morphine keeps the structural specificity of morphine by utilizing ethyl bromoacetate as a cross-linking agent to activate the morphine. Through the adoption of a sandwich immune method, the using amount of antigens is effectively reduced, and the immunization time is shortened. The hybridoma cell strain MOP1.0 is high in secretion yield, and the anti-morphine monoclonal antibody obtained through secretion of the hybridoma cell strain MOP1.0 has the characteristics of high affinity, high specificity and high sensitivity. A detection product provided by the invention is more advantageous in the aspects of sensibility, specificity, detection limit and the like.

The invention belongs to the field of organic synthesis, and particularly relates to a synthesis method of imidazo[1,2-alpha]pyridyl-3-formic acid. The method comprises the following steps: reacting N,N-dimethylformamidodimethyl acetal with 2-aminopyridine at 40-100 DEG C for 2-8 hours to obtain an N,N-dimethyl-N'-2-pyridylformamidine intermediate, reacting the intermediate with ethyl bromoacetate at 50-160 DEG C, and recrystallizing to obtain a pure product; carrying out hydrolysis reaction on ethyl imidazo[1,2-alpha]pyridyl-3-formate in a certain solvent for 1-5 hours; and after the reaction finishes, neutralizing with hydrochloric acid, filtering, washing with water, and drying to directly obtain the imidazo[1,2-alpha]pyridyl-3-formic acid pure product. The method has the advantages of accessible reaction raw materials, reasonable price, mild reaction conditions and simple after-treatment, and is easy to operate and control; and the product has the advantages of stable quality and high purity.