Anti-morphine monoclonal antibody, cell strain for generating anti-morphine monoclonal antibody, morphine detection kit and manufacturing method thereof

Abstract

The invention provides a hybridoma cell strain MOP1.0 capable of generating an anti-morphine monoclonal antibody, wherein the cell strain is preserved in China Center for Type Culture Collection with a preservation number of CCTCCNO. C2013159. The invention also provides the anti-morphine monoclonal antibody secreted by the hybridoma cell strain MOP1.0. The invention also provides a kit for detecting morphine in saliva. The invention further provides a detection method and a manufacturing method of the morphine detection kit. The hybridoma cell strain MOP1.0 has the benefit that the artificial antigen of obtained morphine keeps the structural specificity of morphine by utilizing ethyl bromoacetate as a cross-linking agent to activate the morphine. Through the adoption of a sandwich immune method, the using amount of antigens is effectively reduced, and the immunization time is shortened. The hybridoma cell strain MOP1.0 is high in secretion yield, and the anti-morphine monoclonal antibody obtained through secretion of the hybridoma cell strain MOP1.0 has the characteristics of high affinity, high specificity and high sensitivity. A detection product provided by the invention is more advantageous in the aspects of sensibility, specificity, detection limit and the like.

Description

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AI Technical Summary

Benefits of technology

This patented technology uses different techniques to create modified forms of opium called methoxyborylate (MOB) or acetylenesulfonimide (ASI). These modifications can be made without affecting their function or properties during manufacturing processes. By adding these modifying agents at certain sites on the molecule they improve stability while also making them easier to produce. Additionally, this modification allows for increased efficiency when producing large amounts of pure labeled material compared to previous methods such as ELISA. Overall, this innovative technique simplifies the purification step required before obtaining desired materials from natural sources.

Problems solved by technology

This patented describes different methods or techniques for identifying substances called morphines from liquids found naturally throughout life without causing harmful side effects. These include collectors made up of natural materials like salt solutions, but they also contain chemical compounds associated with their effectiveness. Additionally, these processes involve extracting specific parts of tissues containing certain components through various means, resulting in contamination issues. Therefore, current analyte assays require complicated steps involving multiple fluorescence tests before results may appear positive. There is currently no suitable solution available due to lack of sufficient accuracy and ease of administration during emergency situations.

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