Anti-Kratom monoclonal antibody and kit for detecting Kratom
A technology for cloning antibodies and anti-kratom monoclonals, which can be used in biological testing, measuring devices, and material inspection products. It can solve problems such as doping or contamination, and achieve high sensitivity, high affinity, and high specificity.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0066] Preparation of card pain antigens, including the following steps:
[0067] Preparation of a fully antigen of a capumn wood base:
[0068] a) Protect the amino group: dissolve the cap column wood base in an organic solvent, stirred at room temperature, under an ice water bath, inert gas protection, slowly drop the caporial carnoid gas amount of 1-3 times trifluoroacetic anhydride, withdraw the ice Water bath, room temperature, stirring reaction 2-4h; After the reaction, the solvent was evaporated under reduced pressure in a water bath at 40-50 ° C to give a light yellow oil liquid, and 5-15 ml of n-hexane dissolved, crystallized into a white solid in an ice water bath. Hisherache is washed with white solid;
[0069]
[0070] b) Hydrolyzed ester group: Add 5-15 mL of methanol and 1-3 ml of water to the obtained white solid, then add 5-10-fold mole of lithium hydroxide, and continue to react 20h after stirring; the reaction is completed, with a thin plate Analysis, raw mater...
Embodiment 1
[0111] Example 1: Preparation of Cardiac Antigen
[0112] Preparation of a capillarkine (Antigen1) complete antigen:
[0113] a) Protect amino group
[0114] 398.5 mg (1 mmol) cap column wooden base powder was added to 150 ml of three flasks, 60 ml of dichloromethane, stirred at room temperature, under ice water bath, under nitrogen protection, slowly add 420 mg (2 mmol) trifluoroacetic anhydride, The ice water bath was removed, stirred at room temperature for 3 hours. After the reaction, the evaporation of the solvent was evaporated under a water bath at 45 ° C to give a pale yellow oily liquid, and 10 ml of n-hexane dissolved. Crystallization in an ice water bath can be crystallized into a white solid. 420 mg (0.86 mmol) of the white solid was washed with n-hexane.
[0115] b) hydrolyzate
[0116] 10 mL of methanol and 2 ml of water were added to the white solid, and 158 mg (6.88 mmol) lithium hydroxide was added, and the dissolution was continued for more than 20 hours.
[0117...
Embodiment 2
[0136] Example 2: Preparation of Cardial Antigen
[0137] Preparation of a capillarkine (Antigen1) complete antigen:
[0138] a) Protect amino group
[0139] 398.5 mg (1 mmol) capillaine powder was added to 150 ml of three flasks, 60 ml of dichloromethane was added, and the mixture was stirred at room temperature, under nitrogen protection, slowly add 210 mg (1 mmol) trifluoroacetic anhydride, The ice water bath was removed, stirred at room temperature for 3 hours. After the end, the solvent was evaporated under a water bath at 405 ° C to give a pale yellow oily liquid, and 10 ml of n-hexane dissolved. Crystallization in an ice water bath can be crystallized into a white solid. 239 mg (0.6 mmol) of the white solid was washed with n-hexane.
[0140] b) hydrolyzate
[0141] 10 ml of methanol and 2 mL of water were added to the white solid, and then 110 mg (4.8 mmol) lithium hydroxide was added, and the reaction was continued for more than 20 hours after stirring dissolution.
[0142...
PUM
Property | Measurement | Unit |
---|---|---|
Affinity constant | aaaaa | aaaaa |
Affinity constant | aaaaa | aaaaa |
Affinity constant | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com