A kit for quantitative detection of C-reactive protein in giant pandas and its application.

By optimizing the giant panda CRP antigen, preparing highly specific polyclonal antibodies and constructing a detection scheme, the problems of long detection time and high cost in existing giant panda CRP detection technologies have been solved. This enables rapid and convenient quantitative detection and reference value establishment, which is suitable for giant panda disease monitoring and diagnosis.

CN121899420BActive Publication Date: 2026-05-26CHINA CONSERVATION & RES CENT FOR THE GIANT PANDA SICHUAN
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
CHINA CONSERVATION & RES CENT FOR THE GIANT PANDA SICHUAN
Filing Date
2026-03-24
Publication Date
2026-05-26

AI Technical Summary

Technical Problem

Existing technologies cannot provide an efficient, simple, and low-cost kit for the quantitative detection of C-reactive protein in giant pandas. Furthermore, existing methods are time-consuming and cumbersome, failing to meet the needs of disease monitoring and treatment in giant pandas.

Method used

By optimizing the CRP antigen, a simple detection method was developed, a highly specific, low-cross-reactivity-risk giant panda CRP-specific polyclonal antibody was prepared, and a rapid, simple, and scalable detection protocol suitable for clinical use was constructed, including the use of reagents R1 and R2.

Benefits of technology

Rapid quantitative detection of CRP in giant pandas was achieved, with results available in 10 minutes. Continuous quantification of multiple samples was performed, overcoming the problems of long cycle time and cumbersome procedures in existing methods. A reference value for normal CRP concentration in giant pandas was established, and significantly elevated CRP levels can serve as an auxiliary diagnostic marker for infection or inflammation.

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Abstract

This invention discloses a kit for the quantitative detection of C-reactive protein (CRP) in giant pandas and its application, belonging to the field of biodetection technology. The kit disclosed in this invention comprises reagent R2 with the following concentrations: 50 mM PBS pH 7.5, Tween-20 6 ml / L, 270 nm polystyrene carboxylated latex 2.5 g / L, sucrose 30 g / L, BSA 10 g / L, rabbit anti-giant panda CRP polyclonal antibody 6.75 mg / L, and preservative 0.5 ml / L. The rabbit anti-giant panda CRP polyclonal antibody is obtained by immunizing giant white rabbits with the recombinant antigen expressed by the sequence shown in SEQ ID NO. 4. This invention provides a highly efficient, simple, low-cost, and scalable detection method.
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Citation Information

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