64results about How to "Sensitivity advantage" patented technology

The invention discloses an anti-influenza A virus nucleoprotein monoclonal antibody with high affinity and high specificity, its preparation and application. The anti-influenza A virus nucleoprotein monoclonal antibody is secreted by a hybridoma cell strain H1N1-2F10, and the preservation number of the cell strain is CCTCC C201119. The invention also provides a kit prepared with monoclonal antibody for rapid detection of influenza A. The kit has the characteristics of simple operation, strong specificity and high sensitivity, etc.

The invention discloses a small leakage detection system for a liquid pipeline and relates to the technical fields of tests not included in other classes, acoustic wave measurement and pipeline systems. On the premise of not changing technological processes of current pipelines, a leakage detection ball is sent out by a pipe cleaner ball sending device and flows along with a medium in the pipeline; meanwhile, acoustic signals inside the pipeline and state information of the leakage detection ball are acquired, stored and tagged with time labels; after the leakage detection ball is collected back from a ball collecting bucket, the small leakage detection system is connected with an upper computer, so that the acoustic information stored in the pipeline and the state information of the leakage detection ball are obtained; the obtained acoustic information is directly displayed in an oscillograph on the upper computer; except a ball sending bucket and the ball collecting bucket, if an acoustic amplitude is higher than that of noise in the pipeline, the corresponding time is judged to be the leakage point and recorded; according to the recorded time of the leakage point, the state information of the leakage detection ball is subjected to quadratic integral so as to obtain displacement of the leakage detection ball relative to the ball sending bucket, namely the leakage position. The small leakage detection system for the liquid pipeline can detect small and even tiny leakages and is high in sensitivity.

The invention belongs to the field of petroleum exploration and development and particularly relates to a system and a method for detecting by applying a Raman spectrum technology in the gas detection process of oil-gas logging. A Raman spectrum gas detection system comprises a sample pretreatment device, a Raman gas analyzer, a data signal processing module, a data communication module, a signal output display unit and a master control unit, wherein the sample pretreatment device is connected with the Raman gas analyzer and is used for inputting the pretreated gas in the Raman gas analyzer for analyzing components; the Raman gas analyzer is used for inputting the analyzed result to the data signal processing module; the data signal processing module is used for outputting the analysis result to the signal output display unit through the data communication module; and the master control unit is used for controlling the inputting of sample gas.

The invention discloses a method for measuring formaldehyde and acetaldehyde content in paper for a cigarette at the same time, which comprises the following steps of: preparing a mixed standard stock solution; preparing a standard working solution; creating a standard working curve; preparing a derivatization reagent; preparing a sample solution; analyzing by ultra high performance liquid chromatography; and calculating a result. According to this method, a sample processing method and the chromatographic condition are improved and optimized, so that two compounds to be measured have good separation effect and good peak shape. A scanning operation is carried out by employing two wavelength channels at the same time, so that this method has obvious advantage in sensitivity in comparison with single wavelength measurement at the same time. The ultra high performance liquid chromatography is employed, so that analysis time is greatly shortened. The two compounds to be measured both have better chromatographic peak separation and better linear correlation, the detection limit of the formaldehyde is 0.57mg / kg and the detection limit of the acetaldehyde is 0.34mg / kg. In the method, the recovery rate of the formaldehyde is 98.14%, the recovery rate of the acetaldehyde is 97.55% and the average relative standard deviations of the formaldehyde and the acetaldehyde are both less than 2.5%.

The invention relates to a detection agent composition for living cells, and specifically relates to a detection agent composition for epithelial tissue tumor cells. The composition contains 0-5% of folic acid, 0-10% of a folic acid compound, 0.01-5% of methylene blue, 0.1-10% of a sugar reducing agent, 2-6% of acetic acid and 3-95% of water. The invention also relates to a preparation method of the detection agent composition and a kit containing the detection agent composition.

The invention discloses a strain sensor made of carbon nanotube / epoxy resin composite material. The strain sensor comprises a strain body and conductors arranged at two ends of the strain body, wherein the strain body is made of the carbon nanotube / epoxy resin composite material, and the carbon nanotube / epoxy resin composite material mainly comprises an epoxy resin agent A, carbon nanotubes and a curing agent B. The strain sensor has the advantages that the ratio of the resistance change rate to the strain can reach above 20, the strain can be accurately measured without a digital electric bridge strain gauge extremely high in precision, and the strain sensor is high in sensitivity.

The invention provides a method for detecting gene fusion, and in particular provides a RACE-like PCR (polymerase chain reaction) joint sequencing detection method (RACE-like PCR joint sequencing method for short) for detecting fusion genes such as anaplastic lymphoma kinase (ALK) fusion gene and the like. By the method, different ALK fusion genes can be detected simultaneously, and unknown ALK fusion genes are detected under the condition of not knowing genes fused with ALK and the fusion modes of the ALK fusion genes in advance, so that the sensitivity, practicability and universality of the detection method are improved.

The invention discloses a three-electrode type Pb(II) and Cu(II) electrochemical sensor, a preparation method and application of the electrochemical sensor in concentration detection of Pb(II) and Cu(II) in a liquid environment, and belongs to the technical field of heavy metal ion sensors. The sensor consists of a reference electrode AgCl / Ag, a counter electrode Pt sheet and a glassy carbon working electrode coated with an electrode modification material. ZIF-67, MWCNT and Nafion are used as working electrode modification materials. The ZIF-67 is rich in coordination defects and has good catalytic activity, the MWCNT has good conductivity, the Nafion is used as a selective permeable membrane, adsorption of heavy metal ions can be further enhanced, and the purpose of enhancing the mechanical stability of the sensor in a liquid environment is achieved. The sensor is an electrochemical sensor, is small in size and simple to operate, and is suitable for field analysis and detection. The prepared sensor has a good recovery rate in the detection of an actual water sample, which proves that the sensor has application potential in an actual environment.

The invention provides a method for displaying latent fingerprints by adopting fluorescent dye-modified nano particles. By the adoption of organic fluorescent dye chemical modified nano particles, fluorescence excitation of a normal light source during fingerprint displaying and multi-waveband fluorescence emission are realized; residues with doubtful fingerprints are put into a uniformly vibrated displaying agent, then unfixed dyes are washed off by clean water, and the residues are naturally dried; when the residues are observed under a long-wave ultraviolet lamp, a high-contrast fluorescence characteristic can be clearly seen. According to the method, the technical difficulties of interference, such as object light reflection, during latent fingerprint displaying, low sensitivity to powder displaying, low combination property, limited application range and the like are solved; the method is easy to operate and does not have toxic and side effects.

The invention provides a cognitive load and pressure measurement method based on peripheral vessel strain. The cognitive load and pressure measurement method comprises the following steps: collecting PPG (photo-plethysmography) signals by adopting a transmission-type or reflection-type photoelectric sensor, and carrying out filtering to eliminate noises; carrying out instantaneous peripheral vessel strain feature extraction; carrying out dynamic sliding window arrangement and data modulation based on the statistics to obtain the continuous and stable sVRI output; and finally, evaluating the cognitive load and the pressure level by adopting sVRI. The invention further provides a corresponding measurement device. Different from the traditional measure methods based on the peripheral vessel expansion degree, in the measurement method provided by the invention, the sVRI adopts the ratio of the amplitudes of the adjacent wave bands of the PPG signals, and the experiment finds that the indication is relatively sensitive and reliable in reflecting the cognitive load and the pressure; the cognitive load and pressure measurement technology based on the peripheral vessel strain has the obvious advantages in the aspects of sensitivity, reliability and individual differences when used for measuring the psychological activities including the cognitive load and the psychological pressure of people, can carry out real-time measurement, and can be well applied to the pervasive environment.

The invention discloses a primer and probe for early detecting methylation of a lung cancer gene. The primer and probe comprise a primer pair and a probe for detecting methylation of a CDO1 gene, wherein the primer pair has the sequences as shown in SEQID No:1 and SEQID No:2, and the probe has a sequence as shown in SEQID No:3. The clinical detection sensibility of the primer and probe is greaterthan 77.6%, and the specificity is greater than 98%. The invention also discloses a reagent kit based on the primer and probe. The reagent kit is simple to operate and low in reagent cost.

The invention discloses a fluorescence probe and a synthetic method thereof and an application of the fluorescence probe in circulating tumor cell detection. The fluorescence probe comprises a fluorescence group, a cathepsin B substrate and folic acid molecules, the fluorescence group is connected with the cathepsin B substrate through a self-degradation bond, and then is connected with the folic acid molecules; the fluorescence group is excited by the excitation light under a dissociation state, and then is enable covalent connection with other molecules for quenching. The fluorescence probe is the switching-mode fluorescence probe having a spontaneous cascade amplification function, can be used for avoiding complex operation steps of antibody or nucleic acid detection during a circulating tumor cell detection process, is used for an automatic biochemical analyzer as a matching reagent, is in favor of promoting automation detection of tumor cells, and has the advantages of simple and convenient operation, short time consuming, and low apparatus requirement. The fluorescence probe can be used for positioning minimal tumor cells and tubercle in clinical operation, and has wide application prospect and large market value.

The invention relates to a method for achieving infrared detection by a desorption phenomenon of infrared excitation molecules. The method comprises the following steps that micro-nanometer structure surfaces absorb chemical molecules; infrared signals are added to the micro-nanometer structure surface, and the chemical molecules adsorbed by the micro-nanometer structure surface are desorbed from the micro-nanometer structure surfaces under excitation of the infrared signals; micro-nanometer structures has changes of physical properties due to the molecular desorption, and the changes of the physical properties of the micro-nanometer structures are detected through a device, and therefore detection of the extra infrared signals is achieved. Due to an effect of the extra infrared signals, the chemical molecules adsorbed on the micro-nanometer structure surfaces are desorbed from the micro-nanometer structure surfaces because the chemical molecules absorb the infrared signals to make the temperature rise, the optical property, electrical property, magnetic property and other physical properties of the micro-nanometer structures is caused to change, and therefore the phenomenon is utilized to achieve the detection of the infrared signals. Compared with existing infrared detection technologies, the method for achieving the infrared detection by the desorption phenomenon of the infrared excitation molecules has the advantages that the sensitivity is high and feedback signals are easy to analyze and the like.

The invention discloses a dopamine electrochemical sensor based on an Au / Ti3C2Tx modified glassy carbon working electrode and a preparation method of the dopamine electrochemical sensor, and belongs to the technical field of biosensors. The sensor is composed of a reference electrode AgCl / Ag, a counter electrode Pt sheet, a glassy carbon working electrode modified by Au / Ti3C2Tx, an electrolyte of 0.1 M, and a phosphate buffer solution with a pH value of 7. According to the invention, Au / Ti < 3 > C < 2 > T < x > is used as a working electrode modification material, and Ti < 3 > C < 2 > T < x > has a large specific surface area, excellent conductivity, a hydrophilic surface and good biocompatibility, and has a good adsorption effect on dopamine; au nanoparticles are used for catalyzing the redox reaction on the surface of the electrode, so that the conductivity, the electron transfer capability and the electrochemical active area are improved. The electrode modification material is a nano composite material, and the purpose of improving the sensitive characteristic of the sensor is achieved by utilizing the synergistic effect among the composite materials.

The invention relates to the technical field of humidity detection sensors, in particular to a humidity sensor of a CeO2 / g-C3N4 hybrid membrane as well as a preparation method and application thereof.The humidity sensor comprises a substrate, a humidity-sensitive material combined on the substrate and a resistance value detection device communicated with the humidity-sensitive material, wherein the humidity-sensitive material is a cerium oxide-graphite carbonitride hybrid membrane prepared from cerium oxide and graphite carbonitride. The humidity sensor of the CeO2 / g-C3N4 hybrid membrane hasa wider humidity monitoring range, and when the relative humidity (RH) is increased from 0% to 97%, the response value of the sensor is close to 7000. Compared with a traditional humidity sensor, thesensor has obvious advantages in the aspects of sensitivity, stability, responsiveness, repeatability, recovery time and the like.

The invention discloses to a small leakage detection ball for a liquid pipeline and relates to the technical fields of tests not included in other classes, acoustic wave measurement and pipeline systems. The small leakage detection ball for the liquid pipeline comprises a battery and power supply switching circuit, an electret microphone and a signal processing circuit, an STM32 (scanning tunneling microscope 32) processor, an inertial navigation module, a timing unit, a magnetic field transmitting device, an SD (secure digital) card memorizer, a USB (universal serial bus) interface and a Jtag (joint test action group) interface of the electret microphone, wherein an output end of the electret microphone is connected with the signal processing circuit which is connected with the STM32 processor, the STM32 processor is connected with the inertial navigation module, the timing unit, the magnetic field transmitting device, the SD card memorizer, the USB interface and the Jtag interface, and the battery and power supply switching circuit is connected with all the circuits to supply power to the same. The small leakage detection ball for the liquid pipeline can detect small and even tiny leakages and is high in sensitivity.

The invention discloses a gamma camera special for mammary glands based on CZT. The gamma camera comprises a main case, an overhauling cabinet door, a working table, casters, an electric pushing rod, a CZT detector, a cantilever structure, a display support and a display. The overhauling cabinet door is arranged outside the main case, the working table is arranged on one side of the upper portion of the main case, and the cantilever structure is arranged on the other side of the upper portion of the main case; the display support is arranged on the working table, the display is arranged above the display support, and the casters are arranged below the main case; the gamma camera also comprises a lifting guiding rail, the lifting guiding rail is installed above the cantilever structure, a supporting plane is installed at the bottom of the lifting guiding rail, and the CZT detector is installed above the supporting plane and is capable of being installed on the lifting guiding rail in a sliding mode. According to the gamma camera special for the mammary glands based on the CZT, a supporting face plate is arranged below the CZT detector and used for fixing and slightly extruding a breast, artificial moving of the mammary glands is prevented, the thickness of the breast is reduced, and lesion points are more easily detected through the detector.

The invention discloses an RT-LAMP method for identifying Japanese encephalitis virus gene types I and III. Through the comparative analysis of Japanese encephalitis virus gene nucleotide sequences of gene types I and III, four specific primers for specific amplification of Japanese encephalitis virus nucleic acids of gene types I and III are designed for six regions on a target gene, the reaction result can be determined according to the color change of liquid in a reaction tube when a reaction is finished after calcein fluorochrome is added to a reaction system, and the reaction result can be determined according to whether a precipitate exists in the reaction tube or not after the reaction is finished or whether a ladder band appears or not during electrophoresis. According to the method, under the constant temperature condition, reverse transcription and nucleic acid amplification are realized within 1h, and the Japanese encephalitis virus gene types I and III can be quickly detected and identified within 1h.

The invention discloses a high-sensitivity optical fiber grating acceleration sensor based on a rigid hinge. The high-sensitivity optical fiber grating acceleration sensor comprises an optical fiber,a package shell, a base fixed on an inner bottom surface of the package shell, and a pin shaft, two oscillating bars and mass blocks which are fixed on a base; one end of each oscillating bar can hinge with the base in a way of shaking up and down through the pin shaft, thereby forming rigid hinge, and the other end is fixed on the mass block; two oscillating bars and the mass block are symmetricabout the pin shaft center line; when adopting a single optical fiber, the optical fiber is horizontally fixed on the highest point or lowest point of two mass blocks; when adopting two optical fibers, two optical fibers are horizontally fixed at the highest point and the lowest point of two mass blocks; the part between two fixation points on the optical fiber is the optical fiber work section, gratings are arranged at two sides of the pin shaft center line on the optical fiber work section, the optical fiber work section has a certain prestress and cannot contact with other parts; the mass blocks are free from contacting with the package shell, and two ends of the optical fiber are connected to an interrogator after penetrating through the package shell. The sensor disclosed by the invention can realize the precise measurement of the micro-vibration.

The invention provides a preparation method of robot flexible touch control material. Firstly piezoelectric ceramic powder and conductive polymer powder are mixed to be ground and dispersed and then evenly laid between upper and lower polyimide films, the 1mmx1mm array mode is formed by controlling the concussion frequency, and the flexible touch control film composite material is acquired by appropriate heating and calendering formation. According to the technology, the common piezoelectric ceramics is used as a pressure sensor, and the effect of connecting the piezoelectric ceramics and conducting charges can be realized by selecting the conductive polymer so that the pressure signals of 1mm2 resolution can be acquired; besides, the whole sensing equipment has flexibility, low preparation cost, stable signal acquisition, great material environment stability and long service life.

The invention relates to an intelligent cereal drying device based on solar energy. The intelligent cereal drying device consists of four parts of a vacuum pipe type solar energy heat collector, a heat exchanger, a gravity circulation type additional heating stock bin and a controller, wherein the gravity circulation type additional heating stock consists of inner walls, a housing, a spiral lifter, far infrared heating pipes, a blowing machine and a wind pipe; the controller consists of parts of a single-chip microcomputer, a temperature-detecting control circuit, a humidity detecting controlcircuit, a solar energy heat collector heat exchanging valve switching circuit, a keyboard and LCD display circuit, a pressure sensor and a circuit thereof, an alarm circuit, a far infrared compensating heater and a heating pipe piston valve; and the humidity detecting control circuit comprises a moisture-sensitive sensor; and the moisture-sensitive sensor is a zirconia moisture-sensitive sensor based on doping of metal ions.

The invention provides a portable gas explosive identification device based on a Raman spectrum, aiming at solving the problem that most of existing gas explosive identification devices are inconvenient to carry and use due to too large volume. The portable gas explosive identification device based on the Raman spectrum comprises a sample pretreatment device, a first analysis unit, a second analysis unit, a data communication unit, a signal output display unit and a main control unit. The portable gas explosive identification device provided by the invention has the beneficial effects that an analysis and processing mechanism is not included in the identification device, so that the reduction of volume and the improvement of the portability of the identification device are facilitated; combustible gases such as methane can be detected, rapid, simple and noninvasive quantitative and qualitative analysis can be achieved, and the portable gas explosive identification device has obvious advantages in aspects of continuity, reliability, accuracy, sensitivity and the like; and the safety of gas explosives, namely combustible gases, in inspection and identification processes can be ensured to the greatest extent.

The invention provides a reagent kit for diagnosing oral squamous cell carcinoma. The reagent kit comprises specific antibodies for detecting HOXA10, specific antibodies for detecting HOXA11, specific antibodies for detecting HOXC6 or compositions with more than one or two optional specific antibodies for detecting the expression quantities of HOXC8. The reagent kit has the advantages that sequencing results for mRNA [messenger RNA (ribonucleic acid)] of the oral squamous cell carcinoma and normal oral tissues in TCGA (the cancer genome atlas) databases are statistically analyzed, data of 32 cases of normal oral tissues and 341 cases of oral squamous cell carcinoma tissues are incorporated, obvious difference of expression of partial genes in HOX families in the oral squamous cell carcinoma and the normal tissues is discovered, and accordingly the reagent kit is suitable to be applied to diagnosing the oral squamous cell carcinoma; expression of genes in the HOX families is detected in oral squamous cell carcinoma tissue pathological sections by the aid of immunohistochemical processes, and accordingly positive expression of the genes in the oral squamous cell carcinoma and application values of the genes in diagnosis can be determined.

The invention discloses a method for preparing self-rotation microscopic cantilever detectors based on electronic beam plasma countermark, and firstly, a substrate is plated with a silicon dioxide membrane by a low temperature and low pressure chemical LPCVD method; secondly, a plasma body RIE erodes the SOI substrate plated with the silicon dioxide membrane for eroding window arrays; thirdly, the substrate is wet-etched, and an alkaline solution is used for eroding plasma opened WAFER windows; fourthly, a window Si membrane is coated with a photo-resistant adhesive; fifthly, an oscillator shape is prepared by EBL, the electronic beams are exposed, and an electronic microscopic erosion system MASH is masked and eroded; sixthly, the oscillator microscopic shape is carved by eroding and removing the Si (parts without the photo-resistant adhesive) by the plasma body RIE; and finally, a whole oscillator microscopic shape is carved by eroding and removing the photo-resistant adhesive on the surface of the detector by the plasma body RIE. The detector prepared by the invention is characterized by high quality factor, high resolution, good stiffness coefficient, compact structure, high sensitivity and good working reliability parameter.

The invention discloses a method for detecting genotoxic impurities of diethyl sulfate and diisopropyl sulfate in a calcium dobesilate capsule by a GC-MS (Gas Chromatography-Mass Spectrometer) method. According to the method, diethyl sulfate and diisopropyl sulfate which are high in boiling point react with the derivatization reagent to generate iodoethane and iodoisopropane which are low in boiling point and easy to gasify, and the derivatization treatment operation of the method is simple; the content of genotoxic impurities of diethyl sulfate and diisopropyl sulfate in the calcium dobesilate capsule is indirectly measured through a GC-MS method, and compared with an HPLC method, the method has obvious advantages in sensitivity. The analysis methodology verification result of the method shows that the specificity, sensitivity, precision, accuracy and linearity of the method accord with the verification guidance principle of the correlation analysis method, and each evaluation index is excellent.

A prediction method of abnormal operation in cloud cluster environment, includes obtaining a set of tasks from the original set of tasks; extracting static features from the task set; dividing the tasks into normal tasks and abnormal tasks by support vector machine, calculating the dynamic characteristics of normal tasks and forming a dynamic characteristic sequence; using the improved unit gatedrecurrent neural network to process the dynamic feature sequences of multiple instant simultaneously on-line, retrieving the ID of the exception task, stopping the operation of the exception task if the identical ID is retrieved, and marking the job to which the exception task belongs as an exception job. The abnormal operation prediction method under the cloud cluster environment provided by theinvention is obviously superior to other methods in terms of accuracy and prediction time, so that the abnormal operation energy consumption can be effectively controlled.