66 results about "Cyclooctene" patented technology

The invention belongs to the technical field of pharmaceutical preparations, and relates to traditional Chinese medicine self-assembled nanoparticles for enhancing anti-inflammatory activity as well as a preparation method and application of the traditional Chinese medicine self-assembled nanoparticles. The traditional Chinese medicine self-assembled nanoparticles are prepared from biphenyl cyclooctene type lignan and an oleanane type pentacyclic triterpenoid compound, wherein the mass ratio of the biphenyl cyclooctene type lignan to the oleanane type pentacyclic triterpenoid compound is 1 to (0.125 to 8). Through an anti-solvent exchange method, the carrier-free nanoparticles based on self-assembly of the traditional Chinese medicine active ingredients are prepared by utilizing spontaneous non-covalent interaction between the biphenyl cyclooctene lignan and the oleanane pentacyclic triterpenoid, so that the cytotoxicity is remarkably reduced, the biocompatibility is enhanced, and the toxic and side effects are reduced; meanwhile, the traditional Chinese medicine self-assembled nano-particles have a remarkable cellular uptake effect, the absorption and utilization efficiency of the medicine is improved, and more efficient anti-inflammatory biological activity is shown.

The invention discloses a method for synthesizing degradable polyethylene through copolymerization of conjugated lactone and cycloolefin. The preparation method comprises the following steps: catalyzing ring-opening metathesis polymerization of alpha, beta-unsaturated lactone and a cycloolefin monomer by using a Grubbs catalyst, hydrogenating the obtained copolymer to prepare a series of polyethylene-like materials with different densities introduced into ester groups in a main chain, and simultaneously retaining the thermal and mechanical properties of high-density polyethylene; after the polyethylene material is degraded and recycled through alcoholysis, polyethylene-like with high molecular weight and excellent performance can be obtained again through a polycondensation method, and the problem that an existing polyethylene material is difficult to recycle is solved. The synthesis method is simple and convenient to operate, the polymer obtained by copolymerization of conjugated lactone and cyclooctene is high in molecular weight, and ester groups in a main chain are uniformly distributed. Regioselectivity of conjugated lactone polymerization is good, and an AB type monomer obtained after alcoholysis is beneficial to polycondensation to obtain a high-molecular-weight polymer. The hydrogenated product is pure white solid powder, has few metal catalyst residues, and is expected to replace a bulk polyethylene material.

The invention discloses an organic phosphorescent material intermediate for an OLED (Organic Light Emitting Diode) as well as a preparation method and application of the organic phosphorescent material intermediate, and relates to the field of organic phosphorescent materials. The intermediate is prepared through a Diels-Alder cycloaddition reaction between trans-cyclooctene and 3, 6-diphenyltetrazine, the reaction is carried out under the conditions that the temperature is 20-30 DEG C and the time is 10-30 minutes, and the mass ratio of the trans-cyclooctene to the 3, 6-diphenyltetrazine is 1: (1.0-2.5). Trans-cyclooctene is prepared by isomerizing cis-cyclooctene under the irradiation of 254nm ultraviolet light in the presence of a photosensitizer; the 2, 3, 6-diphenyl tetrazine is prepared by condensation, cyclization and purification of benzaldehyde and benzoyl hydrazine. The purity of the prepared intermediate reaches up to 99.2%, the yield reaches 82.4%, the intermediate serving as an OLED luminescent material has excellent thermal stability, and the quantum efficiency is still kept at 90.1% after HTHH treatment is conducted for 1000 h. The invention provides a new intermediate choice for developing a high-performance OLED (Organic Light Emitting Diode) material.

Disclosed herein are trans-cyclooctenes (TCOs) that have an improved T-linker. In particular, the TCOs have improved in vitro and in vivo properties as compared to TCOs known from the prior art. The disclosure also pertains to in vivo and in vitro methods of using said trans-cyclooctenes, as well as medical uses thereof, and methods for making said TCOs.

The application discloses a preparation method of a composite antioxidant and application thereof, and belongs to the field of high polymer materials. In the application, a ring octene and a silicon-containing cyclic olefin monomer are subjected to metathesis copolymerization under the action of a metathesis catalyst, a silicon-containing polymer is obtained after chemical hydrogenation, and then the composite antioxidant is obtained after hydrolysis and mixing with lignin. The main chain of the composite antioxidant is a saturated hydrocarbon chain, the compatibility with polyethylene is high, the silicon-containing polymer is hydrolyzed to generate active silanol, the active silanol is efficiently bonded with lignin, and the lignin provides natural polyphenol antioxidant function. The application realizes efficient antioxidant of the composite material, high compatibility and long-term durability, and provides a new idea for high-performance environment-friendly high polymer composite materials.

Thermoplastic compositions include: a. from about 1 wt% to about 70 wt% of a polycarbonate homopolymer component; b. from about 10 wt% to about 40 wt% of a polybutylene terephthalate (PBT) component; c. from about 5 wt% to about 30 wt% of a polycarbonate copolymer component; and d. from about 1 wt% to about 15 wt% of a flame retardant component comprising a cyclic phosphazene compound having an oxaphosphorin ring-containing structure. The compositions have improved heat resistance and flame retardant properties.

The invention discloses a macromolecular antioxidant as well as a preparation method and application thereof, the structural formula of the antioxidant is # imgabs0 #, and R is an antioxidant group; m, n and x are positive numbers; and the antioxidant group is one or more of # imgabs 1 #. The macromolecular antioxidant material is obtained through double decomposition copolymerization and hydrogenation of the olefin monomer containing antioxidant groups and the cyclooctene monomer which are simple and easy to obtain or can be simply prepared, the macromolecular antioxidant material has the performance similar to that of a commercial antioxidant, the molecular structure can be accurately controlled through the ROMP-based method, the molecular weight is high, and the molecular weight is high. After the antioxidant is added into a polyolefin product, the performance including the mechanical property of the polyolefin product is not obviously changed, the polyolefin product has good thermal oxidation stability, and the migration amount of the antioxidant is reduced.

The application discloses application of a divinyl tetrazine in cyclization of a linear polypeptide, and a structure formula of the divinyl tetrazine is; the linear polypeptide has at least two free mercapto groups, and the vinyl groups of the divinyl tetrazine are subjected to Michael addition reaction with corresponding mercapto groups to form a ring. The application provides a new use of the divinyl tetrazine, the divinyl tetrazine can be subjected to high-efficiency Michael addition reaction with two natural cysteine mercapto groups on the linear polypeptide through the vinyl groups, so that double-mercapto peg cyclization of the linear polypeptide is realized, and the purpose of limiting the conformation of the linear polypeptide and improving the stability of the linear polypeptide is achieved. Moreover, the divinyl tetrazine can be subjected to a reverse electron demand Diels-Alder reaction with trans-cyclooctene (TCO) after the two vinyl groups are subjected to reaction with mercapto groups, so that functional polypeptides can be modularly constructed by coupling with functional molecules modified with TCO, and related applications are realized.

The invention relates to the technical field of medical adsorbents for blood perfusion, and discloses an application of a trans-cyclooctene group adsorbent based on a biological orthogonal reaction in preparation of a circulating tumor cell clearing preparation. The adsorbent adopts sephadex microspheres as a carrier, the surface of the carrier is modified with a trans-cyclooctene (TCO) group, a tetrazine (Tz) group is taken in by CTCs through modified mannose, the Tz group is expressed on the surface of a CTCs cell membrane in the form of glycoprotein through a Roseman-Warden pathway in cells, TCO and Tz are subjected to a biological orthogonal reaction specifically, and the adsorption capacity of the adsorbent is improved. The sephadex microsphere adsorbent can effectively enrich and remove CTCs in blood of the whole body of a patient through adsorption, and compared with a conventional single-antibody or multi-antibody capture technology, the sephadex microsphere adsorbent does not depend on antibody-antigen binding, and the capture efficiency of the CTCs can be remarkably improved through a biological orthogonal reaction.

An example of a biological reactant includes a norbornenyl-functionalized or a trans-cyclooctene-functionalized oligonucleotide. The norbornenyl-functionalized oligonucleotide includes a norbornene group attached to a phosphate of a nucleotide attached to a 5' end of a plurality of linked nucleotides. The trans-cyclooctene-functionalized oligonucleotide includes a trans-cyclooctene group attached, via an alkyl chain, to a phosphate of a nucleotide attached to a 5' end of a plurality of linked nucleotides.

The invention discloses a shape memory filter sieve tube thermal expansion composite material and a preparation method thereof. The thermal expansion composite material for the shape memory filter sieve tube comprises the following raw materials in parts by weight: 40-50 parts of polyolefin elastomer POE; 20 to 30 parts of polyethylene; 15 to 20 parts of trans-polyisoprene; 15-25 parts of sodium bicarbonate; 6-8 parts of a foaming agent H; 3-5 parts of zinc oxide; 2-3 parts of stearic acid; 1 to 2 parts of dicumyl peroxide; 5 to 10 parts of polycyclooctene; and 8-15 parts of carbon nanotubes. The raw materials are subjected to high-temperature, mixing and vulcanization treatment to obtain the thermal expansion composite material for the shape memory filter sieve tube. The thermal expansion composite material prepared by the invention has the characteristics of accurate response temperature and smaller pore diameter on the premise of ensuring the porosity, and can realize a higher-precision sand control effect when being used for preparing the sand control expansion screen pipe.

The present disclosure relates generally to trans-cyclooctene modified TPD conjugates comprising a targeting protein degrading agent (TPD) optionally covalently bound to at least one trans-cyclooctene moiety via a linker, for use in, for example, cancer therapy, tumor growth, and immunotherapy.

The invention belongs to the technical field of petroleum and natural gas yield increase, and particularly relates to a preparation method of an anti-shearing polymer fracturing fluid. The preparation method comprises the following steps: preparing a monomer with high strength and shear fracturing resistance from methyl propiolate, aluminum chloride, cyclooctene and other raw materials, adding the monomer into N-vinyl pyrrolidone, 2-acrylamido-2-methylpropanesulfonic acid and acrylamide to prepare a polymer, and mechanically stirring at a high speed to obtain the polymer. And completely dissolving the reaction monomer. And adding an initiator to initiate the monomers to polymerize under the irradiation of an ultraviolet lamp, and reacting at a constant temperature to obtain a product. The synthesis method disclosed by the invention has the characteristics of low cost, high yield and simple preparation process, no by-product is generated, and the obtained product has good anti-shearing performance and has a relatively good application prospect in oil field fracturing and recovery efficiency improvement.

This invention provides a system for in-situ cardiac NO generation based on bioorthogonal reactions and its application, belonging to the pharmaceutical field. The system of this invention improves the bioavailability of NO in the heart, thereby significantly improving myocardial ischemia-reperfusion injury. Specifically, microneedles are pre-implanted into the heart, and then a delivery system (prepared by modifying L-arginine with trans-cyclooctene to form an L-arginine prodrug, and then preparing a nano-delivery system by electrostatic adsorption of L-Arg-TCO) is injected into the body via intravenous injection. Only when the nano-delivery system circulates to the heart, and the TCO undergoes a bioorthogonal reaction with (4-(6-methyl-1,2,4,5-tetraazinecyclo-3-yl)phenyl)methylamine in the microneedles, can the L-arginine prodrug be activated, thereby achieving cardiac-targeted delivery of L-arginine, leading to the enrichment, activation, and conversion of L-arginine into NO in the heart.

The present invention relates to the field of nuclear medicine technology, and discloses a targeted compound bimolecular probe, comprising a component A and a component B respectively labeled with radioactive metal nuclides; component A is a molecular probe targeting fibroblast activation protein (FAP), and the molecular probe targeting fibroblast activation protein (FAP) includes a trans-cyclooctene group (TCO); component B is a molecular probe targeting integrin α v β6 molecular probe, targeting integrin α v The β6 molecular probe includes a tetrazine group (Tz); wherein the radioactivity ratio of component A to component B is (1-2): (2-1). The present invention has the following technical effects: the TCO in the FAP-targeting molecular probe and the integrin α v The Tz in the β6 molecular probe can undergo a bioorthogonal reaction, achieving a tertiary amplification effect, increasing tumor uptake, prolonging the retention time of the targeted compound bimolecular probe, improving the target / non-target ratio, and enhancing nuclear medicine diagnostic and therapeutic effects. The present invention also discloses a method for preparing and using the targeted compound bimolecular probe.

The present disclosure relates to ureido ligands targeting PSMA comprising a moiety targeting PSMA, a linker, and a pyridazine moiety. The disclosed PSMA-targeted ureido ligands are suitable for use as radiopharmaceuticals, as imaging agents (e.g., diagnostic agents), or for the treatment of prostate cancer, or as diagnostic agents.

The application discloses a kind of single-substituted 1,2,4,5-tetrazine compounds based on five-membered heterocyclic ring and preparation method thereof.The single-substituted 1,2,4,5-tetrazine compounds based on five-membered heterocyclic ring keep high reaction rate (10 4 ~10 5 M ‑1 s ‑1 ), improve the LUMO+1 orbital energy of single-substituted tetrazine compound to improve its stability, or improve the reaction ability of tetrazine compound under the same or similar LUMO+1 orbital energy, so that the single-substituted 1,2,4,5-tetrazine compounds based on five-membered heterocyclic ring have higher stability when the reaction rate is certain, have higher reaction rate when the stability is certain, provide a new idea for breaking the limitation of orbital energy and reaction ability with trans-cyclooctene, can promote the retro Diels-Alder reaction of 1,2,4,5-tetrazine compound and trans-cyclooctene in vivo, and is of great significance to the field of medicine.

Disclosed herein include compositions comprising a buffer, and trans-cyclooctenes (TCOs) that may have improved properties, for example in clinical use. In certain embodiments, the TCOs may have improved in vitro and in vivo properties as compared to other TCOs. In certain embodiments, the compositions disclosed herein provide improved stability and / or shelf life. The disclosure also pertains to in vivo methods of using said compositions, as well as medical uses thereof, combinations comprising said TCOs, and / or methods of preparing said compositions.

The present disclosure relates to compositions and methods useful for the functionalization of surfaces in the absence of a metal catalyst. The compositions include compounds of formula (I), which react with strained alkene-containing compounds, before or after molecular assembly catalyzed by tyrosinase, to afford cycloadducts. The strained alkene-containing compound may further comprise any molecule of interest, including small molecules and macromolecules, thereby enabling surface functionalization. In certain embodiments, the strained alkene-containing molecule comprises a moiety selected from the group consisting of a trans-cyclooctene (TCO), cyclopropene, cyclobutene, and norbornene.

The invention relates to a (cyclooctenyl)silane or an isomer mixture of (cyclooctenenyl)silanes as comonomer(s) which are used for production of a polyoctenamer and to the uses thereof in vehicle tyres.

Described herein are nucleic acid complexes for the targeted delivery of reversibly capped mRNA complexes (procapped mRNA) which are conditionally activated in the presence of a selected cell signal. The mRNA complexes can include mRNA with is 5' capped with a selectively removable protecting group involving a click-release reaction between tetrazine and trans-cyclooctene (TCO), to achieve selective activation of mRNA within targeted cells.

The present invention discloses a new class of frans-cyclooctenes (TCOs), "a- TCOs," that are prepared in high yield via stereocontrolled 1,2-additions of nucleophiles to trans-cyclooct-4-enone, which itself was prepared on large scale in two steps from 1,5-cyclooctadiene. Computational transition state models rationalize the diastereoselectivity of 1,2-additions to deliver a-TCO products, which were also shown to be more reactive than standard TCOs and less hydrophobic than even a trans- oxocene analog. Illustrating the favorable physicochemical properties of a-TCOs, a fluorescent TAMRA derivative in live HeLa cells was shown to be cell-permeable through intracellular Diels-Alder chemistry and to washout more rapidly than other TCOs.

The application discloses a thermosetting resin and a preparation method thereof, and the preparation method comprises the following steps: (1) preparing a prepolymer solution of monomer I and a prepolymer solution of monomer II respectively; the monomer I is a mixture of dicyclopentadiene and ethylidene norbornene; the monomer II is a mixture of dicyclopentadiene and cyclooctene; (2) under a heating condition, preparing a laminated material formed by pre-curing the prepolymer solution of the monomer I and the prepolymer solution of the monomer II; and (3) performing heat treatment on the laminated material obtained in the step (2) to obtain the thermosetting resin. The application is based on a soft and hard combined multi-layer strategy to construct a layered thermosetting resin material with rigidity and toughness, the thermosetting resin provided by the application contains dynamic carbon-carbon double bonds between layers, and the interface effect is enhanced by using chemical bond dynamic exchange and physical swelling behavior, so that the thermosetting resin is efficiently toughened.

Examples are directed to composition, kits, and methods thereof. An example composition comprises a protein and an oligonucleotide linked to the protein via a tetrazine and a trans-cyclooctene (TCO) in a predetermined relationship.

The application discloses a kind of photoactivation biotin-biological orthogonal prodrug system and its anti-tumor application.The system integrates biotin targeting ligand, photo-caged tetrazine (TZ) group into one molecule through connecting chain, and can release activated TZ under specific wavelength light, then, TZ and trans-cyclooctene (TCO) modified prodrug or probe occur biological orthogonal reaction, realize the accurate release of tumor site.The system has the functions of active targeting, light-controlled rapid release and diagnosis and treatment integration, and has high tumor inhibition rate, which is significantly better than traditional chemotherapy, and has no obvious systemic toxicity, providing a new strategy for precise treatment of malignant tumors.

The invention discloses thymine-containing polyolefin as well as a preparation method and application thereof, the structural formula of the polyolefin is shown in the specification, x is equal to 100-400, and R is phenyl. Norbornene monomer NBT containing nuclear basic groups is synthesized and is copolymerized with cyclooctene (COE) through ring-opening metathesis polymerization (ROMP), and long-chain alkane is introduced to improve the toughness of the polymer; and a series of polymers with ultra-high molecular weight are synthesized by changing the proportion. The NBT in the polymer enables the polymer to have good hydrophobic performance, and the NBT as a hot melt adhesive has good adhesion performance to different substrates.

The application discloses a method for quickly regulating layer spacing of a layered molecular sieve and application thereof. The method is characterized by using tetraethyl silicate condensate, tetrabutyl titanate silane and ferrocene silane as intercalation reagents, making a carrier gas carry vaporized intercalation reagents to flow through a reactor loaded with the layered molecular sieve, making the carrier gas carry vaporized deionized water to flow through the reactor loaded with the layered molecular sieve, and then performing calcination on the layered molecular sieve to obtain a pillared post-molecular sieve with good thermal stability. The application adopts gas phase pillaring, and has the advantages of simple preparation method, mild conditions, small amount of raw materials, simple steps, high yield, easy scale production, and the like. The intercalation reagent improves the high-temperature tolerance of the original layered molecular sieve, and at the same time, a plurality of active sites and places for adsorption of reactants are introduced at one time. The pillared post-molecular sieve is used in a reaction of preparing epoxycyclooctane by oxidizing cyclooctene with hydrogen peroxide, and the reaction has a cyclooctene conversion rate of 43% or more and an epoxycyclooctane selectivity of 65% or more.

To provide a process for preparing functionalized cyclooctene.SOLUTION: The present disclosure relates to a process for preparing functionalized cyclooctene, and a synthetic intermediate prepared by the process. The present disclosure relates to a method for preparing a functionalized payload composition for delivering a therapeutic drug to a subject, which has improved water solubility, and is to be used by such reaction. The functionalized payload has improved water solubility compared to a payload connected to unsubstituted cyclooctene, and therefore is compounded and administered more easily.SELECTED DRAWING: None

The application discloses a multifunctional backbone molecule for controllable and visual monitoring of drug release and a preparation method and application thereof, the multifunctional backbone molecule has a structure shown in formula I, includes a tetrazine structural unit and a phenanthrene dioxin skeleton, and fluorescence of the phenanthrene dioxin is quenched through fluorescence resonance energy transfer effect between the tetrazine and the phenanthrene dioxin parent nucleus. The multifunctional backbone molecule provided by the application can be subjected to a reverse electron demand Diels-Alder bioorthogonal bond cleavage reaction with a prodrug modified by a trans-cyclooctene, 3-isocyanopropyl or 3-isocyanopropyl-1-carbamoyl group, so that the quenching effect is destroyed, fluorescence is recovered, the prodrug is activated, controllable release and visual monitoring of the drug are realized. In addition, the multifunctional backbone molecule provided by the application can be connected with a targeting group on the phenanthrene dioxin skeleton, so that controllable release of the drug at a target site and real-time visual monitoring of the drug release are realized.

The method for producing a radioactive metal-labeled antibody of the present invention has a step for click reacting a radioactive metal complex with an antibody site-specifically modified by a peptide to generate a radioactive metal-labeled antibody. The click reaction is carried out between a first atomic group with which the radioactive metal complex is equipped and a second atomic group linked directly or indirectly to the peptide. The second atomic group is an atomic group including an azide or an atomic group including trans-cyclooctene.