Preparation method for ketamine artificial antigen

A technology of artificial antigen and artificial hapten, applied in the field of preparation of ketamine artificial antigen, can solve the problems of expensive instruments, inaccessibility, time-consuming detection, etc., and achieve the effects of strong specificity and advanced synthesis technology

Inactive Publication Date: 2016-09-28
HANGZHOU LAIHE BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, the detection of ketamine mainly relies on high-performance liquid chromatography (HPLC), gas chromatography (GC), thin-layer chromatography (TLC), simple (MS), etc., but

Method used

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  • Preparation method for ketamine artificial antigen
  • Preparation method for ketamine artificial antigen
  • Preparation method for ketamine artificial antigen

Examples

Experimental program
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Embodiment 1

[0036] (1) Preparation of artificial hapten---Synthesis of KET hapten:

[0037] (a) Put 100mg of ketamine hydrochloride into a beaker, add 10ml of deionized water, then slowly add ammonia water to adjust the pH value to 11;

[0038] (b) adding 50ml of dichloromethane for extraction, a total of 3 extractions, and collecting the organic phase for later use;

[0039] (c) drying with anhydrous magnesium sulfate, filtering, and evaporating the solvent under reduced pressure to obtain free ketamine;

[0040] (d) Dissolve free ketamine in 5ml of acetone, add 60μl of ethyl bromoacetate and 150mg of potassium carbonate, reflux at 80°C for 20h, cool to room temperature, and filter to obtain a filtrate;

[0041] (e) The filtrate is distilled under reduced pressure to obtain the ester of ketamine; take 50 mg of the ester of ketamine, 1 ml of tetrahydrofuran, and 1.5 ml of methanol and mix evenly, add 5 ml of 1 M sodium hydroxide solution, and react at room temperature for 20 h;

[0042]...

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Abstract

The invention provides a preparation method for a ketamine artificial antigen. The preparation method comprises the first step of hapten preparation and detection, wherein an ester group is introduced through reaction with ethyl bromoacetate, hydrolysis is carried out under the alkaline condition, and ketamine hapten containing carboxy groups is obtained; the second step of artificial antigen preparation and detection, wherein the ketamine hapten and bovine gamma globulin (BGG) are combined through a carbodiimide method to prepare the ketamine artificial antigen, namely, ketamine-BGG. The prepared ketamine artificial antigen can be used for animal immunization, a corresponding ketamine antibody is obtained, the preparation method can be used for studies of various kinds of ketamine immunoassays, and a more convenient, quicker and more accurate approach is provided for ketamine detection.

Description

technical field [0001] The invention belongs to the technical field of biochemistry, and in particular relates to a method for preparing an artificial antigen of ketamine. Background technique [0002] Ketamine ( Ketamine, KET), pharmaceuticals The full name is ketamine hydrochloride, also known as ketamine, and it has many aliases, such as ketamine, ketamine, K powder or Kzai, etc. It is generally used as an anesthetic in clinical practice. The molecular formula is (C 13 h 16 CINO), the structural formula is as follows: [0003] [0004] Ketamine is a derivative of phencyclidine, the basic structure of which is cyclohexylamine. Introduced into clinical practice in 1965 by Domino et al., it is an anesthetic with definite analgesic effect among non-barbiturate intravenous anesthetics. As a medicine, there are mainly two dosage forms of injection and tablet. Ketamine hydrochloride is a white crystalline powder, odorless. Soluble in water, soluble in hot ethanol, inso...

Claims

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Application Information

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IPC IPC(8): C07K14/47C07C221/00C07C225/20
CPCC07K19/00C07C221/00C07K14/4717C07C225/20
Inventor 叶肖俊
Owner HANGZHOU LAIHE BIOTECH CO LTD
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