A kind of preparation method of eddp artificial antigen

Abstract

The invention provides a preparation method for an artificial antigen of a methadone metabolite-2-ethylidene-1,5-dimethyl-3,3-diphenyl pyrrolidine (hereinafter referred to as EDDP). The method comprises the following steps: firstly, performing preparation and detection on a hapten, wherein diphenylacetonitrile and 2-chloro-N,N-dimethyl propylamine hydrochloride are taken as raw materials, and an addition reaction, an esterification reaction, a cyclization reaction, a nitratlon reaction and a Friedel-Crafts reaction are performed to obtain the hapten containing carboxyl; secondly, performing preparation and detection on the artificial antigen, wherein the hapten is combined with bovine gamma globulin (BGG) through a carbodiimide method to prepare the EDDP artificial antigen, namely EDDP-BGG. The prepared EDDP artificial antigen can be subjected to animal immunization to obtain a corresponding EDDP antibody, and can be used for making a research on various methadone immune analysis methods; a relatively convenient, quick and accurate way is provided for detection of methadone.

Description

technical field [0001] The invention belongs to the technical field of biochemical engineering, and in particular relates to a method for preparing an artificial antigen of methadone metabolite (EDDP). Background technique [0002] Methadone, also known as Mesantol, is a narcotic analgesic. Its chemical structure is far from that of morphine, but its pharmacological effects are very similar to morphine. Its prominent features are strong analgesic effect, effective oral administration, long-lasting inhibitory effect on withdrawal symptoms of opioid drug addicts, continuous and effective repeated application, and suitable for detoxification treatment of addicted patients. [0003] The metabolic site of methadone is mainly in the liver, and the metabolic pathway is demethylation on the nitrogen atom, and then undergoes self-cyclization to obtain the main metabolite 2-acetal-1,5-dimethyl-3,3-diphenyl Dipyrrolidine (EDDP), the structural formula is as follows: [0004] [00...

AI Technical Summary

Problems solved by technology

However, the following problems are often encountered when detecting the prototype of methadone in patients' urine: (1) a small number of patients have a particularly strong metabolizing ability for methadone. Because they have a particularly strong ability to metabolize methadone, the concentration of prototype methadone in urine is too low to detect methadone, so the government will think that patients have not taken methadone and force them to take methadone again. Taking too much methadone will not only affect the curative effect, At the same time, it brings greater side effects to patients; (2) Another part of people escapes the detection of the government by adding a small amount of methadone to their urine and getting a positive result (immune products based on the detection of the original form of methadone). of methadone is marketed in exchange for opioids

[0007] At present, the detection of methadone metabolites (EDDP) mainly relies on high-performance liquid chromatography (HPLC), gas chromatography (GC), thin-layer chromatography (TLC), simple (MS), etc., but there are expensive instruments, time-consuming detection, and Professional and technical personnel are required to operate, which cannot meet the fast and accurate requirements of modern detection

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