Preparation method of methadon artificial antigen

Abstract

The invention provides a preparation method of methadon artificial antigen. The preparation method comprises the following steps: step 1: preparation and detection of hapten: 2-chlorine-N, N-dimethyl propyl amine and diphenyl acetonitrile are taken as raw materials, hydroxy is led to a position 1 through condensation, oxidation, esterification and reduction, reaction with butanedioic anhydride iscarried out for obtaining hapten containing carboxyl; and step 2: preparation and detection of artificial antigen, the methadon artificial antigen, namely methadone-bovine serum albumin, is enabled to be prepared through the combination with BSA (bovine serum albumin) by a carbodiimide method. The prepared methadon artificial antigen can be used for animal immunization, so that corresponding methadon antibody can be obtained; and the method is applicable to researches on various methadon immunoassay, so as to provide a more convenient, quicker and more accurate way for the detection of methadon.

Description

technical field

[0001] The invention belongs to the technical field of biochemical industry, and in particular relates to a preparation method of methadone artificial antigen. Background technique

[0002] Methadone (MTD), also known as methadone, phenaladin, and amidone, belongs to narcotic analgesics and is a μ opioid receptor agonist. Its drug effect is similar to that of morphine. Pupil, sedation and other effects. Compared with morphine, it has the characteristics of longer action time and lower drug dependence. It was a narcotic analgesic synthesized in Germany during World War II to replace morphine. Its structural formula is:

[0003]

[0004] Methadone has physiological dependence, psychological dependence and tolerance. Therefore, methadone should be used rationally, and its abuse may lead to the same consequences as heroin. Methadone is also the object pursued by drug addicts, so it must be strictly managed. It has been included in the norms of narcotic dr...

Examples

Embodiment 1

[0035] (1) Preparation of artificial hapten:

[0036] (a) Weigh diphenylacetonitrile (103.5mmol), 2-chloro-N,N-dimethylpropylamine hydrochloride (165.6mmol) into a 150ml single-necked round bottom flask, add a stirring bar, and stir at 100°C React for 6 hours; after the reaction, the reaction solution was extracted with 3×50ml ether, the ether phase was extracted with 3×40ml of 5% hydrochloric acid solution, the obtained hydrochloric acid phase was adjusted to alkaline with 25% sodium hydroxide, and then 3×50ml Extracted with ether, dried with anhydrous potassium carbonate, filtered and dried, and recrystallized with 10ml of ether to obtain 10.58g of white solid product I. Thin-layer chromatography was performed with ethyl acetate as the chromatographic fluid to detect the product IR f =0.5~0.7.

[0037](b) In a 50ml single-necked round bottom flask, add 1.958ml of 70% sulfuric acid solution, heat to 100°C, add 2g of the product from the previous step, raise the temperature...

technical field

[0001] The invention belongs to the technical field of biochemical industry, and in particular relates to a preparation method of methadone artificial antigen. Background technique

[0002] Methadone (MTD), also known as methadone, phenaladin, and amidone, belongs to narcotic analgesics and is a μ opioid receptor agonist. Its drug effect is similar to that of morphine. Pupil, sedation and other effects. Compared with morphine, it has the characteristics of longer action time and lower drug dependence. It was a narcotic analgesic synthesized in Germany during World War II to replace morphine. Its structural formula is:

[0003]

[0004] Methadone has physiological dependence, psychological dependence and tolerance. Therefore, methadone should be used rationally, and its abuse may lead to the same consequences as heroin. Methadone is also the object pursued by drug addicts, so it must be strictly managed. It has been included in the norms of narcotic dr...

Examples

Embodiment 1

[0035] (1) Preparation of artificial hapten:

[0036] (a) Weigh diphenylacetonitrile (103.5mmol), 2-chloro-N,N-dimethylpropylamine hydrochloride (165.6mmol) into a 150ml single-necked round bottom flask, add a stirring bar, and stir at 100°C React for 6 hours; after the reaction, the reaction solution was extracted with 3×50ml ether, the ether phase was extracted with 3×40ml of 5% hydrochloric acid solution, the obtained hydrochloric acid phase was adjusted to alkaline with 25% sodium hydroxide, and then 3×50ml Extracted with ether, dried with anhydrous potassium carbonate, filtered and dried, and recrystallized with 10ml of ether to obtain 10.58g of white solid product I. Thin-layer chromatography was performed with ethyl acetate as the chromatographic fluid to detect the product IR f =0.5~0.7.

[0037](b) In a 50ml single-necked round bottom flask, add 1.958ml of 70% sulfuric acid solution, heat to 100°C, add 2g of the product from the previous step, raise the temperature...

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