114 results about "Methyl xanthine" patented technology

A pharmaceutical formulation comprises a plurality of seamless minicapsules having a diameter of from 0.5 mm to 5 mm, at least some of the minicapsules containing a methyxanthine as one active ingredient, and at least some of the minicapsules containing a corticosteriod as another active ingredient.

The invention provides compositions containing a fraction isolated or derived from hops and a methylxanthine. The invention additionally provides compositions containing a fraction derived from hops and a curcuminoid. The invention also provides methods of using such compositions to reduce inflammation.

Personal care composition comprising from about 0.05% to about 5% of at least one aquaporin-stimulating compound selected from the group consisting of xanthine, caffeine; 2-amino-6-methyl-mercaptopurine; 1-methyl xanthine; 2-aminopurine; theophylline; theobromine; adenine; adenosine; kinetin; p-chlorophenoxyacetic acid; 2,4-dichlorophenoxyacetic acid; indole-3-butyric acid; indole-3-acetic acid methyl ester; beta-naphthoxyacetic acid; 2,3,5-triiodobenzoic acid; adenine hemisulfate; n-benzyl-9-(2-tetrahydropyranyl)adenine; 1,3-diphenylurea; 1-phenyl-3-(1,2,3-thiadiazol-5-yl)urea; zeatin; indole-3-acetic acid; 6-benzylaminopurine; alpha-napthaleneacetic acid; 6-2-furoylaminopurine; green tea extract; white tea extract; menthol; tea tree oil; ginsenoside-RB1; ginsenoside-RB3; ginsenoside-RC; ginsenoside-RD; ginsenoside-RE; ginsenoside-RG1; ginseng root extract; ginseng flower extract; pomegranate extract, extracts from Ajuga turkestanica; extracts from viola tricolor and combinations thereof; an additional ingredient selected from the group consisting of niacinamide, glycerin and mixtures thereof, and a dermatologically-acceptable carrier.

There is provided the use of a methylxanthine derivative such as theophylline and a steroid in a synergistic combination for the treatment of chronic obstructive pulmonary disease, wherein the combination is administered by the inhaled route for pulmonary delivery.

The present invention relates to the combination of a methylxanthine and a carbonic anhydrase activator to provide synergistic effects. The invention further relates to the improved/enhanced cognitive ability of individuals, particularly those suffering from various disorders, such as Alzheimer's Disease, stroke, hypoxia, general dementia, ADHD, mental retardation, and "sun down" syndrome.

A pharmaceutical or veterinary composition, comprises a first active agent selected from a dehydroepiandrosterone and/or dehydroepiandrosterone-sulfate, or a salt thereof, and a second active agent comprising a methylxanthine derivative for the treatment of asthma, chronic obstructive pulmonary disease, or any other respiratory disease. The composition is provided in various formulations and in the form of a kit. The products of this patent are applied to the prophylaxis and treatment of asthma, chronic obstructive pulmonary disease, or any other respiratory disease.

A composition for human consumption, comprising creatine and creatinine, the latter being in sufficient quantity to render creatine in an aqueous medium substantially stable, and a methyl xanthine; and a method of making the composition is provided.

Dietary supplements comprising Citrus aurantium extract and a methylxanthine, with or without St. John's wort extract and L-Phenylalanine for controlling weight wherein fat is lost and lean body mass preserved.

An anti-inflammation substrate for decreasing the proinflammation induced by the cytokines and inhibiting the lung function degeneration is provided. The anti-inflammation substrate includes one selected from the group consisting of a 7-[2-[4-(2-chlorobenzene)piperazinyl]ethyl]-1,3-dimethylxanthine, a 7-[2-[4-(4-nitrobenzene)piperazinyl]ethyl]-1,3-dimethylxanthine, a respective pharmaceutical acceptable salt thereof, and a combination thereof.

The present invention provides a mesenchymal stem cell adipogenic differentiation culture medium, and belongs to the technical field of stem cells. The mesenchymal stem cell adipogenic differentiation culture medium comprises a DMEM/F12 culture medium, and further comprises FBS with a volume percentage of 5-50%, glutamine with a volume percentage of 0.5-5%, antibiotic with a volume percentage of 0.5-5%, 50-500 [mu]M indometacin, 50-500 nM insulin, 5-50 nM dexamethasone, 0.5-5 [mu]M 3-isobutyl-1-methylxanthine, and 0.05-0.5 [mu]M fasudil hydrochloride. The mesenchymal stem cell adipogenic differentiation culture medium of the present invention has advantages of high inducing efficiency and the like.

The invention relates to the technical field of medicines and particularly relates to an industrial preparation process of linagliptin. The industrial preparation process comprises the steps of adding a reactant a (2-chloromethyl-4-methyl-quinazoline), an equal molar ratio of reactant b (8-bromo-7-(2-butynyl)-3-methylxanthine), an acid-binding agent and a proper amount of solvent into a reaction kettle to react at 0-140 DEG C for 3-8 hours, after TLC detection reaction is finished, directly adding a reactant c ((R)-3-phthalimide piperidine-tartaric acid) and an acid-binding agent, namely N,N-diisopropylethylamine without processing a reaction mother liquid to react at 0-125 DEG C for 3-10 hours, after the TLC detection reaction is finished, adding ethanolamine without processing the reaction mother liquid to react for 2-10 hours, after the TLC detection reaction is finished, dropwise adding purified water, carrying out suction filtration to obtain a linagliptin rough product, and refining by virtue of a refining method disclosed in a patent CN101437823A, so as to obtain a linagliptin refined product. According to the industrial preparation process, linagliptin is synthesized by virtue of a one-pot continuous feeding method, the consumption of the solvent is low, and the operation is easy; and the industrial preparation process is suitable for industrial production.

Provided is a megakaryocyte and/or platelet production method, enabling to produce a megakaryocyte and/or platelet from mesenchymal cells such as preadipocytes in a relatively short period of time, simply, in a large amount and at lower cost or more efficiently in vitro and a method for producing TPO simply and in a larger amount. A first invention is a method for producing a megakaryocyte and/or platelet, comprising culturing a mesenchymal cell in a mesenchymal cell culturing basic medium containing an iron ion and an iron transporter and collecting megakaryocytes and/or platelets from a culture. A second invention is a method for producing thrombopoietin, comprising culturing a mesenchymal cell or mesenchymal cell-derived megakaryocyte in a mesenchymal cell culturing basic medium containing an iron ion and an iron transporter and collecting thrombopoietin from a culture. A third invention is a method for producing thrombopoietin, comprising culturing a preadipocyte in a preadipocyte culturing basic medium containing dexamethasone, 3-isobutyl-1-methylxanthine and insulin and collecting thrombopoietin from a culture.

The invention discloses a preparation method for Trajenta. The Trajenta is a high-purity Trajenta which is prepared by taking a raw material 8-bromo-7-(2-butynyl)-3-methylxanthine, namely a compound a as basic material, selecting appreciate reaction material ratio, reaction time and reaction conditions in substitution, deprotection and other reaction steps. The preparation method has the beneficial effects that a novel method for preparing the Trajenta is provided, and dimer impurities generated in the reaction process are effectively avoided, so that the high-purity Trajenta is obtained, the raw material of the reaction are easily available, the production cost is low, and industrial production requirement of the Trajenta can be met to the greatest extent.

The invention relates to a separation, culture and induction differentiation method for precursor adipocyte in chicken muscle and belongs to the technical field of cytology. According to the method, hormone mixture 3-isobutyl-1-methylxanthine, dexamethasone and insulin are used, an induction differentiation system of external-source oleic acid and rosiglitazone is added, generation of oil drop ofprecursor adipocyte in the chicken muscle is induced, and an effective research means can be provided for the quality of poultry meat and particularly fat deposition in poultry muscle. By adopting themethod, intramuscular precursor adipocyte which is even in component and exuberant in proliferation and differentiation can be obtained, the effective induction differentiation system of adipocyte inthe chicken muscle is obtained, and the method has the advantages that the method is simple and convenient, easy to operate, low in cost, high in vigor and excellent in proliferation and differentiation capacity and can achieve acquirement of a large number of cells.

The invention provides a new method for preparing a theobromine by a 3-methyl xanthine disodium salt methylation with more production value. The theobromine is prepared by using acetone as a solvent to react with a dimethyl sulfate under the existence of a sodium carbonate and then acidified. The invention uses the acetone as the solvent and decreases the hydrolysis loss of the dimethyl sulfate; the sodium carbonate provides an alkaline condition for the methylation reaction and the Ph value is intermediate. The dimethyl sulfate is dropped to carry out the methylation reaction with the advantages of good selectivity and a yield higher than other methylation technique by more than 10 percent. The acetone is recycled and reused, the reaction is relatively complete and has little caffeine by-product, and the acquired analysis content of theobromine crude product HPLC is larger than 90 percent. The invention is extremely applicable to industrial production.The invention relates to a refining method of santheose, which is characterized in that dissolves liquid alkali into crude santheose solution, decolourizes, fitlers, and adds reduction agent into filter liquor, acidifies at 60-80 DEG C until pH=5-6, filters and dries to obtain santheose final product. The inventive method has refining yield of 90%, standard product color, high product quality, low cost and industrialization suitability.

The invention discloses a method for purifying preadipocytes derived from fetal bovine skeletal muscle tissue. According to the method, a used material is longissimus dorsi tissue of a 3-month-old bovine fetus, and used reagents contain collagenase, fetal bovine serum, the low- glucose Dulbecco's Modified Eagle's Medium, a cell sorting buffer solution, a PDGFR-alpha (platelet-derived growth factor receptor alpha) antibody, dexamethasone, 3-isobutyl-1-methylxanthine, bovine insulin and oil red O.

The invention relates to a simple preparation method of high-purity linagliptin. The method includes the steps of making 8-bromine-3-methyl xanthine and 1-bromo-2-butyne react, directly adding 2-chloromethyl-4-methylquinazoline without processing after reaction is completed, preparing a key intermediate 8-bromine-7-(2-butyne-1-yl)-3,7-dihydro-3-methyl-1-[(4-methyl-2-quinazolinyl)methyl]-1H-purine-2,6-diketone of linagliptin through a one-pot method, making the intermediate react with (R)-3-piperidinamine dihydrochloride after being filtered and separated to obtain a linagliptin solution, and obtaining a linagliptin pure product after processing the linagliptin solution. The key intermediate is prepared through the one-pot method, operation is convenient, and yield is increased; the key intermediate reacts with (R)-3-piperidinamine dihydrochloride after being separated, and therefore high-purity linagliptin is obtained, and the requirements for production and declaration of pharmaceutical enterprises are met to the maximum extent.

The invention relates to the field of medicine, and discloses a culture medium for inducing adipogenic differentiation of muscle derived stem cells of skeletal muscles, and an application and an adipogenic differentiation method thereof. The culture medium disclosed by the invention comprises 3-isobutyl-1-methylxanthine, insulin, indomethacin, dexamethasone, pioglitazone and FBS in a basic culture medium. The components of the culture medium are optimized and are induced to differentiate, and the conventional component in the existing mesenchymal stem cells is replaced by pioglitazone, so that an effect of significantly improving the adipogenic differentiation rate of the muscle derived stem cells of skeletal muscles is realized.

In one aspect, a formulation comprising cannabinoids, paracetamol, methylxanthines, salicylates, terpenes, humulus oil, or amino acids individually or any combination or omission thereof for the reduction, alleviation, elimination, and/or suspension of effects of THC exposure and anxiety. The formulation can be the effective amount of cannabinoid is between 5 mg and 5000 mg. The formulation can be the cannabinoid comprises a Cannabidiol (CBD), a cannabidolic acid (CBDA), a Cannabinol (CBN), a Cannabigerol (CBG), a Cannabichromene (CBC), a Cannabicyclol (CBL), a Cannabivarin (CBV), a Tetrahydrocannabivarin (THCV), a Cannabidivarin (CBDV), a Cannabichromevarin (CBCV), a Cannabigerovarin (CBGV), a Cannabigerol monomethyl ether (CBGM), a Cannabielsoin (CBE), or a cannabicitran (CBT). The formulation can be the effective amount of paracetamol is between 0 mg-1000 mg.

The invention provides a new method for preparing theobromine by 3-methyl xanthine disodium salt methylation with more production value. The theobromine is prepared by using acetone as a solvent to react with dimethyl sulfate under the existence of sodium carbonate and then acidified. The invention uses the acetone as the solvent and decreases the hydrolysis loss of the dimethyl sulfate; the sodium carbonate provides an alkaline condition for the methylation reaction and the pH value is moderate. The dimethyl sulfate is dripped to carry out the methylation reaction with the advantages of good selectivity and a yield higher than other methylation technique by more than 10 percent. The acetone is recycled and reused; the reaction is relatively complete and has little caffeine by-product; the acquired analytic content of theobromine crude product HPLC is larger than 99 percent; therefore, the invention is extremely applicable to industrial production.

The invention discloses an environment-friendly refining method of theobromine, and relates to the technical field of synthetic chemistry. The method comprises the following steps: (1) dissolving a crude product to prepare a sodium salt; (2) carrying out decoloration; and (3) carrying out acidification and crystallization. According to the method provided by the invention, the theobromine in a theobromine crude product is converted into a sodium salt by adding a sodium hydroxide solution and insoluble impurities are filtered out; then, the decoloration effect is ensured by use of a decolorant,and pigment impurities are removed; and then, by controlling the pH value during acidification, the residual rate of theobromine in crystallization mother liquor can be reduced and the purity of theobromine in precipitated crystals can be increased. The prepared finished product of theobromine is white or creamy in appearance, and the residual rate of 3-methylxanthine is less than 0.15% or more,and meets requirements of the quality standard of theobromine; and the refining yield reaches 95% or more, thus reducing the loss amount of theobromine in a refining process.

The invention belongs to the technical field of cell culture, and in particular relates to a method for efficient separation and culture of human primary melanin cells. According to the method provided by the invention, an ROCK inhibitor is added to a culture medium which consists of the following ingredients: a Ham's F-12 medium, dibutiryl cyclic adenosine monophosphate, 3-isobutyl-1-methylxanthine, sodium orthovanadate, phorbol 12-myristinate 13-acetate, fetal calf serum and double-antibody; on the basis of mutual actions of the various ingredients, the melanin cells can achieve cloning growth, so that a culture cycle can be shortened by half and separation efficiency of the melanin cells can be greatly improved; the separation and culture method provided by the invention is simple and easy to operate and is low in amount of medium ingredients; and large-scale production of the human primary melanin cells is achieved.