114 results about "Methyl xanthine" patented technology

The present invention provides methods and compositions for the consistent and quantitative differentiation of human preadipocytes isolated from adipose tissue into adipocytes bearing biochemical, genetic, and physiological characteristics similar to that observed in isolated primary adipocytes. The methods of the invention comprise incubating isolated human preadipocytes, plated at least about 25,000 cells / cm2, in a medium containing, glucose, a cyclic AMP inducer such as isobutylmethylxanthine or forskolin, a glucocorticoid or glucocorticoid analogue, insulin or an insulin analogue and a PPARγ agonist or a RXR agonist. The compositions of the invention include media for the differentiation of human preadipocytes, human adipocytes differentiated by the methods of the invention and transfected adipocytes.The present invention also provides methods for determining the ability of a compound to affect the differentiation of human preadipocytes to adipocytes, for determining the ability of a compound to act as a PPARγ antagonist. a glucocorticoid, a glucocoticoid analogue, or an insulin analogue, for transfecting cultured human adipocytes, and as a means to identify novel polypeptides secreted from human adipocytes into the conditioned medium. The methods and compositions have use in the drug discovery of compounds having relevance to the disease states of diabetes, obesity, and cardiovascular disease and in the studies of these diseases.

There is provided the use of a methylxanthine derivative such as theophylline and a steroid in a synergistic combination for the treatment of chronic obstructive pulmonary disease, wherein the combination is administered by the inhaled route for pulmonary delivery.

A personal care composition may comprise a member selected from a group consisting of kinetin, zeatin, and combinations thereof; niacinamide; and a dermatologically-acceptable carrier. A personal care composition may comprise an N-acyl amino acid compound, a skin care active, a member selected from a group consisting of kinetin, zeatin, and combinations thereof, and a dermatologically-acceptable carrier. A personal care composition may comprise a hexamidine compound, a skin care active, a member selected from a group consisting of kinetin, zeatin, and combinations thereof, and a dermatologically acceptable carrier.

A process for preparing theobromine by methylating 3-methyl xanthine disodium salt comprises reacting 3-methyl xanthine disodium salt with dimethyl sulfate by using acetone as solvent in the presence of sodium carbonate and acidifying. The present application also provides a process for refining theobromine which comprises dissolving coarse theobromine in liquid alkaline solution, decolorizing, filtering, adding reducing agent in filtrate, and acidifying at 60-80 °C until pH is 5-6, filtering, and drying to obtain theobromine.