Separation, culture and induction differentiation method for precursor adipocyte in chicken muscle

A technology for inducing differentiation and adipocytes, applied in the field of cytology, can solve the problems of inability to directly separate intramuscular adipose tissue, uneven distribution of poultry intramuscular fat, and inability to effectively separate, achieving unbreakable, good sealing, and induction efficiency high effect

Inactive Publication Date: 2018-03-30
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
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  • Claims
  • Application Information

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Problems solved by technology

Unlike general livestock animals (pigs, cattle, sheep), poultry intramuscular fat is unevenly distributed in the tissue and is invisible to the naked eye, so it is impossible to directly separate intramuscular fa...

Method used

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  • Separation, culture and induction differentiation method for precursor adipocyte in chicken muscle
  • Separation, culture and induction differentiation method for precursor adipocyte in chicken muscle
  • Separation, culture and induction differentiation method for precursor adipocyte in chicken muscle

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Embodiment 1

[0038] In the present embodiment, the method for separating, culturing and inducing differentiation of preadipocytes in chicken muscle comprises the following steps:

[0039] 1) Disinfect 18-day-old (or 14-21 days) old Gushi chickens with bromogeramine, bleed the neck to death, soak in 75% (V:V) alcohol solution for 5 minutes, and work aseptically in the cell room Taiwan for the separation of cells;

[0040] Under aseptic conditions, the chest muscle tissue was collected and put into a preheated PBS petri dish, and the blood vessels, fascia and connective tissue visible to the naked eye were removed (the breast skin, subcutaneous fat and connective tissue were removed with sterile scissors and forceps). The lower pectoralis major muscle was cut out with pre-autoclaved ophthalmic scissors, quickly washed twice in PBS buffer containing double antibodies (100U / mL penicillin and 100U / mL streptomycin), and the tissue block was cut into 1mm 3 Then transfer the shredded tissue to a ...

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Abstract

The invention relates to a separation, culture and induction differentiation method for precursor adipocyte in chicken muscle and belongs to the technical field of cytology. According to the method, hormone mixture 3-isobutyl-1-methylxanthine, dexamethasone and insulin are used, an induction differentiation system of external-source oleic acid and rosiglitazone is added, generation of oil drop ofprecursor adipocyte in the chicken muscle is induced, and an effective research means can be provided for the quality of poultry meat and particularly fat deposition in poultry muscle. By adopting themethod, intramuscular precursor adipocyte which is even in component and exuberant in proliferation and differentiation can be obtained, the effective induction differentiation system of adipocyte inthe chicken muscle is obtained, and the method has the advantages that the method is simple and convenient, easy to operate, low in cost, high in vigor and excellent in proliferation and differentiation capacity and can achieve acquirement of a large number of cells.

Description

technical field [0001] The invention relates to a method for separating, culturing and inducing differentiation of preadipocytes in chicken muscle, and belongs to the technical field of cytology. Background technique [0002] The main source of poultry body energy is fat storage, but excessive deposition of subcutaneous fat and abdominal fat will bring more impact on feed conversion rate and meat quality. Negative effects on meat quality (intramuscular fat-IMF) are the main factors affecting meat quality traits. Intramuscular fat (Intramuscular fat) refers to the lipids deposited inside and outside the endomysium or perimuscular membrane, which can affect muscle quality (tenderness, taste, flavor). To a certain extent, the higher the intramuscular fat content, the better the meat quality; however, excessive accumulation of fat in the belly of chickens will reduce feed utilization efficiency, increase processing costs, and cause certain environmental pollution. Carry out re...

Claims

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Application Information

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IPC IPC(8): C12N5/077
CPCC12N5/0653C12N2500/36C12N2500/40C12N2501/33C12N2501/385C12N2501/39C12N2509/00
Inventor 孙桂荣张蒙李东华康相涛李国喜蒋瑞瑞韩瑞丽李转见田亚东
Owner HENAN AGRICULTURAL UNIVERSITY
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