Method for inducing embryo callus of larch, and dedicated culture medium

A technology of larix and callus, applied in the direction of botany equipment and methods, plant cells, plant regeneration, etc., can solve the problems of not being able to meet the needs of large-scale seedlings and no harvest of granules, and achieve great practical application value, good induction effect

Inactive Publication Date: 2007-02-21
INST OF BOTANY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Japanese larix can be propagated by seeds, but the seeds are strong and have obvious abundance and failure years, and there may be no harvest in poor years, which cannot meet the needs of large-scale seedling cultivation.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1. Induction of embryogenic callus of Japanese larch and its effect.

[0017] 1. Preparation of induction medium

[0018] Induction medium: DCR medium is used as the basic medium, and its composition is DCR+vitamin B 1 (VB 1 )0.5mg / L+Vitamin B 6 (VB 6 ) 0.5mg / L+Niacin 0.5mg / L+Inositol 100mg / L+Glutamine 400mg / L+Casein hydrolysate (CH) 500mg / L+2,4-D2mg / L+6-BA 0.5 mg / L + 30g / L sucrose + 7g / L agar, the pH value of the medium is 5.8.

[0019] Among them, the composition of the DCR medium is: macroelements: 340mg / L KNO 3 , 556mg / L CaNO 3 4H 2 O, 400mg / L NH 4 NO 3 , 170mg / L KH 2 PO 4 , 85mg / L CaCl 2 2H 2 O, 370mg / L MgSO 4 ;Trace elements: 6.2mg / L HBO 3 , 8.6mg / L ZnSO 4 ·7H 2 O, 22.3mg / L MnSO 4 ·H 2 O, 0.25mg / L Na 2 MoO 4 2H 2 O, 0.83mg / L KI, 0.25mg / L CuSO 4 ·5H 2 O, 0.025mg / L CoCl 2 ; Iron salt composition: 5.57g / L FeSO 4 , 7.45g / L Na 2 EDTA.

[0020] 2. Induction of Japanese larch callus

[0021] 1. After the immature Japanese larch cones ...

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Abstract

A method for inducing the embryonic callus with high inducing effect and its special culture medium are disclosed. Said special culture medium DCR contains proportionally VB1, VB6, nicotinic acid, inositol, glutamine, hydrolytic casein, 2,4-dichlorophenoxyacetic acid, 6-BA, cane sugar and agar.

Description

technical field [0001] The invention relates to a method for inducing embryogenic callus of larix plants and a special culture medium thereof. Background technique [0002] At present, a variety of angiosperms have been successfully propagated in vitro. However, the asexual reproduction of pines and fir plants is much more difficult than other species. For example, the induction of embryogenic tissues mostly comes from young tissues (such as immature embryos). ); the maturation and germination of somatic embryos strictly depend on the genotype of explants; the induction rate and conversion rate of seedlings are low, so further research is still needed. The induction of embryogenic callus of pine and fir plants is very sensitive to various components and physical and chemical conditions of the medium. So far, there is no effective induction method and effective medium for the embryogenic callus of pine and fir plants, resulting in somatic cells. Studies on embryogenesis and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C12N5/04
Inventor 林金星杨映根郭仲琛汪小雄卢龙斗
Owner INST OF BOTANY CHINESE ACAD OF SCI
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