Method for inducing embryo callus of larch, and dedicated culture medium

Abstract

A method for inducing the embryonic callus with high inducing effect and its special culture medium are disclosed. Said special culture medium DCR contains proportionally VB1, VB6, nicotinic acid, inositol, glutamine, hydrolytic casein, 2,4-dichlorophenoxyacetic acid, 6-BA, cane sugar and agar.

Description

technical field

[0001] The invention relates to a method for inducing embryogenic callus of larix plants and a special culture medium thereof. Background technique

[0002] At present, a variety of angiosperms have been successfully propagated in vitro. However, the asexual reproduction of pines and fir plants is much more difficult than other species. For example, the induction of embryogenic tissues mostly comes from young tissues (such as immature embryos). ); the maturation and germination of somatic embryos strictly depend on the genotype of explants; the induction rate and conversion rate of seedlings are low, so further research is still needed. The induction of embryogenic callus of pine and fir plants is very sensitive to various components and physical and chemical conditions of the medium. So far, there is no effective induction method and effective medium for the embryogenic callus of pine and fir plants, resulting in somatic cells. Studies on embryogenesis and ...