The invention discloses a flame-retardant antibacterial artificial wig hairline, which comprises, by weight, 80-90% of a polyester material, 5-11% of a flame retardant agent, 2.5-6% of an antibacterial agent, 0.3-3% of a dispersing agent, 0.2-0.5% of an antioxidant, and 2-5% of color masterbatch. According to the present invention, the flame-retardant antibacterial artificial wig hairline has characteristics of high simulation degree, flexible hairline, diverse color, flame retardant property, antibacterial property, no fused drop, environmental protection and the like, and can replace the expensive human hairline. The invention further discloses a manufacturing method of the flame-retardant antibacterial artificial wig hairline, wherein the manufacturing method comprises: pre-treating a polyester raw material, carrying out blending granulation on the polyester material, the antibacterial agent, the flame retardant agent, the dispersing agent and the stabilizer by using a double-screw extruder to obtain a flame-retardant antibacterial slice, carrying out melt spinning on the slice, carrying out high-temperature shaping treatment, carrying out washing and drying, and carrying out other steps.