1064results about How to "High practical application value" patented technology

The invention provides a hybridoma cell line 1C11 and an anti-aflatoxin general monoclonal antibody secreted by the same as well as the applications thereof. The hybridoma cell line 1C11 can be used for preparing a high-titer aflatoxin antibody, and a mouse hydroperitoneum antibody is measured to reach 5.12*106 by using an ELISA (Enzyme-Linked Immunosorbent Assay). The anti-aflatoxin general monoclonal antibody has high sensitivity, respectively reaches the IC50 (50% inhibiting concentration) of aflatoxin B1, B2, G1 and G2 to be 1.2, 1.3, 2.2 and 18.0 pg/mL, is the antibody with highest sensitivity among currently reported four aflatoxin antibodies, is used for measuring the total aflatoxin amounts, i.e. the total amounts of the aflatoxin B1, B2, G1 and G2 and has great practical application values.

The invention belongs to the communication technology field and especially relates to a cloud storage data common auditing method possessing a secret key leakage resistance characteristic. The method mainly comprises the following steps of system initialization: blocking processing is performed on a data file so as to generate public and private key pairs of a cloud user and a cloud server; auditing identification generation: the cloud user sends the data file and a label, and a signature set of a data block to the cloud server; auditing challenge response: a third party auditor generates auditing challenge information and sends to the cloud server; the cloud server calculates combination information according to the auditing challenge information, gets signatures together and sends an auditing identification response information to the third party auditor; and the third party auditor verifies validity of the auditing identification response information. The method has advantages that a condition that digital signature forgery and auditing identification response information forgery caused by leakage of a user signature private key can be prevented; and a malicious cloud server is ensured not to generate the forged auditing identification response information to cheat a TPA and to pass through an auditing verification process.

The invention discloses a vascular stent cutting device and a vascular stent cutting method, belonging to the technical field of medical apparatus processing equipment. The vascular stent cutting device comprises an X-X workbench, a platform, a laser cutting head system and a control system, wherein the X-X workbench is a two-dimensional movement feeding platform which is composed of an X-axis linear motor capable of horizontally moving along the X-axis and an X-axis rotating motor capable of rotating around the X-axis; the X-axis rotating motor is provided with a workpiece fixture; the platform is in counter weight with the X-X workbench; the laser cutting head system comprises a laser generator and a Z workbench; and the Z workbench is a moving platform which is composed of a Z-axis servo motor capable of moving along the vertical direction. A processing and cutting device for producing a vascular stent provided by the invention realizes that the production efficiency of the vascular stent is high, and the production cost of the vascular stent is low, so as to conveniently and effectively process the vascular stents with various shapes. The vascular stent cutting device solves the problems existing in a vascular stent processing device that the miniaturization is not enough, the processing precision is not high, the cutting is slow, the heat affected zone is larger, the service life is short, the production cost is high, etc.

The invention discloses a directional calibration target for camera inner and outer parameter calibration. The directional calibration target is characterized by comprising a checkerboard and directional mark patterns, wherein the checkerboard is formed by black squares and white squares in alternate distribution; the directional mark patterns are arranged in a position near the center position of the checkerboard; the directional mark patterns are three mark circular rings or three mark circles; intersection points of any two diagonally connected black squares or intersection points of any two diagonally connected white squares are used as feature corner points of the directional calibration target; the feature corner points are used for camera calibration; and the directional mark patterns are used for judging the rotation direction of the directional calibration target. The directional calibration target has the advantages that in the camera calibration process, a computer can automatically judge the rotation direction of the directional calibration target; the intelligence and the flexibility of the camera calibration are improved; and the calibration complexity is reduced.

The invention discloses a preparation method for a pine-needle-shaped carbon nanotube/carbon fiber conductive network composite carbon material. The preparation method comprises taking fiber of carbon fiber fabric as a growth substrate, and growing carbon nanotubes arranged in high density on the growth substrate. The carbon nanotubes arranged in high density on the growth substrate and the carbon fiber substrate together form the three-dimensional porous pine-needle-shaped carbon nanotube/carbon fiber conductive network composite carbon material. The material is an ideal material for loading an active substance or reaction substance because of possessed high specific surface area, and is capable of inhibiting structure damage caused during reaction because of excellent mechanical strength. Also, because of intersection or overlapping of carbon nanotube and carbon fiber, the integrity of the conductive framework of the composite material is facilitated to be improved, and the composite material possesses wide application prospect in fields of fuel batteries, supercapacitors, lithium-air batteries, lithium-sulfur batteries, organic solar cells and the like.

The invention provides a face tracking method with classification and identification functions, which comprises the following steps: 1. a sequence to be matched of a current frame is F1, F2; a sequence to be matched of a front frame is F1,F2to Fj; 2. Fm of the current frame is taken out in sequence, and an LBP code pattern is calculated; 3. the obtained LBP code pattern is compared with an LBP code pattern of the front frame so that Fn which has the maximal matching degree with the Fm is found out; 4. whether a person is the same person or not is judged according to the matching degree and the central position distance relation; if so, face images of the same of the person are classified into the same type, if not, Fj+1 equal to Fm is added in the FL sequence; 5. the step 2 to the step 4 are repeated until the matching sequence of the current frame is traversed; and 6. the step 1 is back, and the next frame is processed. The invention adopts LBP characteristics with a rotary uniform mode to classify and identify the identity of a person, classifies the same person into one kind, solves the problem that information is lost after the person is sheltered in the traditional face tracking method, expands the function of a face tracking system and improves the application value thereof.

The invention provides a lipase mutant with improved heat stability and a construction method thereof. The lipase mutant with improved heat stability is obtained by subjecting Candida Antarctica lipase B gene to several rounds of fixed point saturation mutagenesis, the Candida Antarctica lipase B having an amino acid residue sequence expressed by SEQ ID No:1, and the lipase mutant having amino acid residue sequences expressed by SEQ ID No:2, SEQ ID No:3 or SEQ ID No:4, each of which consists of 317 amino acids. A method of semi-rational design is utilized in the invention, and a lipase mutantis obtained by subjecting Candida Antarctica lipase B gene to several rounds of fixed point saturation mutagenesis, wherein the mutant includes amino acid mutations D223G, L278M and combination of the two. Expressed by T50<15> and half-life t1/2 at the temperature of 48 DEG C respectively, the heat stability of the lipase mutant is improved, and a high actual application value and broad market prospects are also provided.

The invention discloses a packaging method for high-efficiency vertical coupling interconnection of optical fiber and optical waveguide chip which comprises the following steps of: preparing a silica-based nano waveguide grating and scanning the silica-based nano waveguide grating by applying a scanning electron microscope; preparing a curing packaging material with a low refractive index; preparing a monomode optical fiber; coating an optical anti-reflection film on the surface of the silica-based nano waveguide grating; regulating the position between the monomode optical fiber and the silica-based nano waveguide grating; dispensing an adhesive at the coupling ends of the monomode optical fiber and the silica-based nano waveguide grating; exposing and curing an inner packaged body subjected to adhesive dispensing; preparing a V-shaped optical fiber positioning groove; placing the monomode optical fiber in the V-shaped optical fiber positioning groove; carrying out curing packaging on the monomode optical fiber; exposing and curing the V-shaped optical fiber positioning groove subjected to adhesive dispensing; and testing the finally packaged structure. The invention overcomes the defects of instability and the like of a nano waveguide and grating vertical coupling system on the existing optical integrated chip. The packaged structure provided by the invention has simple structure and high stability, resists external interference and is easy for array integration.

The invention relates to a method for preparing a one-dimensional self-assembly material with a ZnO nanorod array as a template. The method comprises the following steps: the ZnO nanorod array which is used as the template is immersed in a nitrate solution of a transition metal or a rare earth metal, and the ZnO nanorod array undergoes a hydrothermal reaction at 90-200DEG C. Compared with synthetic methods with other templates, the hydrothermal synthetic method with the ZnO nanorod array as the template has the advantages of simple technology, suitableness of the large area preparation and omission of a template removal step, so a difficulty that the film structure can be easily destroyed in the template removal process of the synthetic methods with other templates is solved.

The invention discloses an active power distribution network robust reconstruction method considering node injection power uncertainty. On the basis of performing reasonable analysis and modeling on the output and load demand uncertainties of a distributed power supply by an affine number, an active power distribution network robust reconstruction model, which takes the minimum number of times ofnetwork on-off actions and the lowest network active loss as the target function and takes the network safety operation as the constraint condition, is established. By virtue of the active power distribution network robust reconstruction method, the shortcoming of neglecting of distributed power supply output intermittency and electric vehicle charging in the current power distribution network reconfiguration scheme can be overcome, so that supporting can be provided for safety evaluation of the active power distribution network in the next step.

The invention relates to a heat flow density sensor, in particular to a diaphragm type heat-flow density sensor and a manufacturing method thereof. The diaphragm type heat-flow density sensor comprises a substrate, a first thermal resistant material, a second thermal resistant material, a thermoelectric pile consisting of a thermode A and a thermode B, and an outgoing line; the thermode A and the thermode B are mutually staggered to form the thermoelectric pile; and the thermal resistant materials are covered on the thermoelectric pile, and the thickness of the thermal resistant material on a high temperature end of the thermoelectric pile is smaller than that of the thermal resistant material on a low temperature end of the thermoelectric pile. In the diaphragm type heat-flow density sensor and the manufacturing method thereof, the thermode A and the thermode B are manufactured directly on the substrate of the thermal resistant material in a mode of vacuum ion sputtering, and a thermo electromotive force is measured through the thermoelectric pile formed by connecting the thermocouples in series, so the diaphragm type heat flow density sensor and the manufacturing method thereof have the advantages of high efficiency, lower cost, simple manufacturing, convenient measurement of a heat flow value, higher accuracy and larger practical application value.

A positioning method for indoor mobile robots belongs to the technical field of mobile robot positioning. The invention provides a multisensor fusion asynchronous time of arrival (ATOA) indoor positioning method based on a single ultrasonic sensor, an encoder and an electron compass, and provides a double-layer Kalman filtering (DLKF) data fusion model. Based on the DLKF model, the ATOA method can effectively eliminate accumulated error in dead-reckoning to obtain high precision of positioning, greatly reduces the quantity of ultrasonic sensors for positioning, and effectively overcomes the disadvantage of blind areas in ultrasonic sensor positioning. Based on the DLKF model, the ATOA method can effectively eliminate accumulated error in dead-reckoning to obtain good precision of positioning, and greatly reduces the quantity of ultrasonic sensors for positioning.

The invention belongs to the field of biochemistry analysis technology, and specifically relates to a one-step concentration and demineralization purification treatment method of biological samples by the use of patterning surface. The patterning surface can be widely applied in pretreatment and detection of biological samples. The method comprises the following steps of: constructing sealing patterning surface of hydrophobic-hydrophilic-hydrophobic region on substrate; dropping a polypeptide or protein solution onto the patterning surface prepared by the above step, naturally drying at room temperature to obtain dried sample points; dropping a substrate solution onto the above dried sample points, and naturally drying at room temperature and forming uniform cocrystallization of the sample and substrates on the patterning surface, so as to realize the concentration and demineralization purification treatment of biological samples. By the use of the patterning surface constructed in the invention, in MALDI-TOF MS detection, signal noise ratio, sensitivity and salt tolerance are significantly improved. The method has a great practical application value.

The invention discloses a method for multi-sensor adaptation and real-time data acquisition of a wearable device. The method comprises the following steps that S1 Bluetooth device adaptation, namely a Bluetooth device develops a broadcast packet feature recognition module, a control interface module and a data standardization module; S2 device binding, namely a cloud server establishes a binding relationship between a user ID (Identification) and a device ID in a database; and S3 concurrent real-time acquisition of multi-sensor data, namely a user logs in to the cloud server on a mobile intelligent terminal to obtain a list for the bound devices, the mobile intelligent terminal opens Bluetooth scan and compares the device ID of each Bluetooth device discovered through scanning with the ID of the bound device, a new device thread is created if matching and a connection is established. The method has the beneficial effects that real-time acquisition of the cross-manufacturer multi-sensor data is realized, real-time processing requirements for the multi-sensor data are met, and real-time concurrent acquisition of the multi-sensor data is realized.

The invention relates to a device for improving advanced nitrogen and phosphorus removal by step feed and a method thereof, belonging to the technical field of sewage biological treatment with biochemical method. Aiming at the defects of the prior A/O step feed technology such as that the biological phosphorus can not be synchronously removed, an UCT technology has high energy consumption and more complex operation and the like, the invention combines the UCT technology with the step feed technology, improves the nitration step of the UCT technology into the series run mode of a continuous two-stage A/O technology, and combines with the strategy of the step feeding of each segmental anoxic diacritical point so as to develop the high-efficiency nitrogen and phosphorus removal technology with the effect of denitrifying dephosphorization. A first segment is provided with an anaerobic reactor, a sedimentation tank returns mud to a hypoxia reactor at the first segment, and the hypoxia reactor is added into the return line of mixed liquor of the anaerobic reactor without nitrified liquid internal reflux facilities from an aerobic reactor to the hypoxia reactor. The invention has stable water flow quality and lower energy consumption.

The invention relates to the technical field of networked control systems, in particular to a method for determining a consistent event trigger controller aiming at the condition that a multi-agent system with a leader has denial of service attack (DoS) by utilizing a modal dependent average residence time method, comprises the following steps of: step one, establishing a leader-follower multi-agent system model and a design event triggering condition; step two, establishing a DoS attack model and a switching system model; step 3, designing an event trigger controller and corresponding switching conditions. The average residence time method adopting modal dependence solves the design problem of the consistent event trigger controller in a leader-follower multi-agent system with DoS networkattack.

The invention relates to an analyzing system and an analyzing method for a wireless network optimizing computer. The system consists of a data source, a data importing module, a wireless network performance intelligent analysis module, wireless network coverage state analysis module, geographic information module, a wireless network cell grade accurate analysis GIS (geographic information system) platform and a solution and needed original data exporting module. The method comprises the following steps of: acquiring data source; importing data and transmitting the imported data to the wireless network performance intelligent analysis module and the wireless network coverage state analysis module for analysis processing by using the data importing module; transmitting an analysis result and geographic information to the wireless network cell grade accurate analysis GIS module to obtain a solution; and outputting a network optimizing scheme by using the solution and the needed original data exporting module. Due to the adoption of the system and the method, the performance of an overall network can be rapidly and efficiently improved, problems can be analyzed and solved accurately, the technical threshold of network optimization is lowered and a network optimizing activity can meet and realize the requirement on industrialization.

A high-efficiency nitrogen and phosphorus synchronous removal method in treating low concentration wastewater belongs to the technical field of biochemical process sewage biological treatment. Aiming at the characteristics of low pollutant concentration and low water inlet C/N in low concentration wastewater, and the disadvantages of low carbon source utilization rate, poor effect of nitrogen and phosphorus synchronous removal, high energy consumption, etc. of a traditional A/O technology in treating the sewage, the method of the invention combines the traditional A/O technology and a step feed technology: an A/O technology anoxic segment is changed into a prepositioned pre-anoxic segment and an anaerobic segment by adding a baffle wall in the A/O technology anoxic segment, and sludge flows backwards to the pre-anoxic denitrification segment; and a nitrated stage is improved with an anoxic-aerobic alternative operation mode, and raw water is separately introduced into each anoxic segment and anaerobic segment by part. Through controlling a four part water inlet flow ratio and controlling the DO of a first aerobic segment as 1.5 to 2 mg/L, and the DO of a second aerobic segment and a third aerobic segment as 1.0 to 1.5mg/L, the method of the invention can realize the water inlet carbon source effective utilization rate of the low concentration city sewage reaching 77% and outlet water reaching first level A discharge standards.

A gear transmission multidisciplinary reliability analysis method considering cognition and random uncertainty in an actual engineering design process belongs to the technical field of design optimization and reliability design of mechanical products and includes quantizing the cognition and the random uncertainty of gear modules, tooth number, tooth surface width, distance between bearings and gear diameter caused by the factors of processing, assembly, material characteristics, applied load, external environment and the like, establishing multidisciplinary reliability integrated evaluation index, integrating of the reliability analysis method and a multidisciplinary design optimization strategy, decoupling of a multidisciplinary reliability analysis process, and being based on a functional measurement method. The gear transmission multidisciplinary reliability analysis method considering the cognition and the random uncertainty provides a comprehensive quantitative method, establishes the multidisciplinary reliability integrated evaluation index which is more conformable to engineering practice, integrates a traditional reliability analysis method and the multidisciplinary design optimization strategy, and provides a new thought for reliability analysis of gear transmission products.

The invention discloses an easily-happening landslide prediction method based on remote sensing technology. The easily-happening landslide prediction method comprises following steps of: obtaining remote sensing images, digital altitude data and geological data; constructing a three-dimensional remote sensing interpretation space scene; detailing interpretation regional landslide distribution characteristics; determining key impacting indicators of landslide growth; and carrying out remote image segmentation processing and establishing an easily-happening landslide prediction model. By use of the easily-happening landslide prediction method, prediction of hidden easily-happening landslides is achieved; the easily-happening landslide prediction method is strong in focusing ability, highly reliable, capable of providing more specific and scientific basis for engineering construction, operation maintenance, disaster prevention and reduction and the like, and capable of increasing the level of engineering operation safety and disaster prevention and reduction.

The invention belongs to the field of biological detection and provides a high-sensitivity immunochromatographic test strip for detecting total aflatoxin content quickly, which is characterized by comprising a test strip, wherein a water-absorbing pad, a detection pad, a gold label pad and a sample pad are adhered on one side of the test strip from top down; the pads are overlapped at the connected parts; the detection pad uses a nitrocellulose film as a substrate pad; transverse quality control lines and detection lines are arranged on the nitrocellulose film from top down; the detection lines are coated with aflatoxin B1-bovine serum albumin (AFB1-BSA) coupling and the quality control lines are coated with rabbit anti-mouse polyclonal antibody; and a nano gold-labeled anti-aflatoxin common monoclonal antibody, which is generated by hybrid tumor cell strain 1C11 having a collection number of CCTCC No.C201013, is sprayed on the gold label pad transversely. The test strip is used for detecting total aflatoxin content and has the characteristics of quick detection, simple operation and high flexibility.

The invention discloses a reversible information hiding method of an encryption image based on coding compression. The method comprises steps of dividing an original image into a prediction region and a non-prediction region before encryption, calculating a pixel prediction vale of the prediction region so as to obtain a prediction difference value and carrying out lossless compression on the prediction difference value to obtain compression data; and randomly generating carrier data and carrying out image encryption and reconstruction on non-precision pixels, the compression data and the carrier data. In order to correctly restore the original image, code table hidden information should be determined and by hiding the code table information and the secret information in the carrier data, reversible hiding is achieved. For the carrier image, secret information is extracted in the carrier data; then, the non-prediction pixels and the compression data are decrypted; according to the code table information, the compression data is decompressed so that the prediction difference value is obtained; and finally, according to the non-prediction pixels and the prediction difference value, the original image is restored in a lossless manner. Compared with the prior art, the provided method has quite big hiding capacity; reversible hiding can be completely achieved; and the method has high actual application value.

The invention discloses a medium and long term hydrologic forecasting method based on empirical mode decomposition. The medium and long term hydrologic forecasting method based on empirical mode decomposition comprises the steps that firstly, a hydrologic forecasting model is built according to the following procedures of (101) empirical mode decomposition, wherein empirical mode decomposition is carried out on a hydrologic time sequence s(t) of a forecast drainage basin, (102) kernel principle component analysis, wherein kernel principle component analysis is carried out on n intrinsic mode function components Fj obtained through empirical mode decomposition and a trend item rn, and p main components F'k are extracted, (103) building of a training sample set, wherein the training sample set is built according to the extracted p main components F'k, (104) building of a support vector machine model, and (105) training of the support vector machine model; secondly, annual runoff data of the years needing forecasting are forecast through the built hydrologic forecasting model. The medium and long term hydrologic forecasting method based on empirical mode decomposition is simple in step, convenient to realize, easy and convenient to operate, good in use effect and capable of effectively resolving the problem of low forecasting accuracy of an existing hydrologic forecasting method.

The invention provides an optimization method of a relative orbit transfer path of a spacecraft based on time-fuel optimum control and relates to the optimization method of the relative orbit transfer path of the spacecraft. The optimization method of the relative orbit transfer path of the spacecraft is used for solving the problem that the finite thrust amplitude is not considered in the existing method and the problem that only time optimum or only the fuel consumption is considered in the existing method when a tracking spacecraft is in a relative orbit coordinate system. The optimization method comprises the following steps: firstly, establishing a kinematics and dynamics model as shown in the specification of the relative orbit, and designing control quantities ux, uy and uz applied along three axes respectively; secondly, decoupling the kinematics and dynamics model of the relative orbit into three subsystems; after decoupling into three subsystems, transforming the total performance index as shown in the specification considering transfer time and fuel consumption of the tracking spacecraft into monoaxial performance index as shown in the specification of each axis, and finally obtaining the time-fuel optimal control law as shown in the specification for controlling the tracking spacecraft. The optimization method is suitable for optimizing the relative orbit transfer path of the spacecraft.