The invention belongs to the technical field of genetic engineering technologies, and in particular relates to a quantitative catalyst preparation device for genetic engineering experiments. The invention aims at providing a quantitative catalyst preparation device, which is time-saving and labor-saving, good in catalytic effect of a prepared catalyst and good in safety, and is capable of quantitatively blending catalyst raw materials and water, for genetic engineering experiments. In order to achieve the aim, the invention provides the quantitative catalyst preparation device for the genetic engineering experiments, which comprises a bottom plate, a left side plate, a first sliding rail, a compression cylinder, a right side plate, a first sliding block, a rack, a connecting rod, a piston, a motor, a first rotating shaft, a driving wheel, a gear, and the like, wherein the left side plate, the first sliding rail, the compression cylinder and the right side plate are sequentially arranged on the top of the bottom plate from left to right. The quantitative catalyst preparation device can realize the processes of stirring and adding water during the preparation of the catalyst by using one electrical device, i.e., the motor, and simultaneously enables the catalyst to be prepared in a closed box.