The invention relates to a system and method for determining direct current system fault branch circuits needing to be in in-series connection with alternating currents. The system comprises an on-line monitoring device, wherein the input of the on-line monitoring device is connected with the positive electrode and the negative electrode of a direct current bus and the ground; a plurality of fault locating devices are arranged in various direct current branch circuits respectively, and the on-line monitoring device is connected with each fault locating device through a communication bus. The method comprises the steps that 1) positive electrode voltage to ground U+, negative electrode voltage to ground U- and in-series connected alternating voltage Uac of the direct current bus are detected, and when the Uac is greater than a set value Uaca, the situation that a direct current system has alternating current in-series connection faults is determined; 2) when the U- is less than or equal to delta U, the situation that the alternating currents are connected to the direct current system in series through the positive electrode is determined; when the U+ is less than or equal to the delta U, the situation that the alternating currents are connected to the direct current system in series through the positive electrode is determined; 3) leak currents of all the branch circuits of the direct current system are detected and ordered, the branch circuit having the maximal leak current value is the branch circuit needing to be in in-series connection with the alternating currents. According to the system and method, an alternating current sensor does not need to be additionally arranged, and system cost is saved. Meanwhile, according to the scheme, signal injection does not exist, and therefore increase of ripples of the direct current system will not be caused, the problems that a relay mistakenly moves and a breaker mistakenly trips are solved, and reliability is higher.