47results about How to "Realize separation detection" patented technology

The invention relates to mixed fillers of a liquid-phase chromatographic column and the chromatographic column, and relates to the technical field of analytical chemistry. The mixed fillers of the liquid-phase chromatographic column comprise long-chain alkyl dimethyl silane bonded silica gel and acylamino silane bonded silica gel in a weight ratio of 10:1-1:10, wherein the difference of the mean particle diameter is in a range of between -20 and 20 percent and the difference of the specific weight is in a range of between -20 and 20 percent. In the chromatographic column made by the mixed fillers, the mixed fillers are filled in a column pipe; the outer sides of the two ends of the column pipe are connected with a pipeline through internal thread; the inner sides of the two ends of the column pipe are provided with sieve plates used for preventing the mixed fillers from leaking outwards; one end of the column pipe is provided with a liquid inlet; and a mobile phase enters the chromatographic column from the liquid inlet. The characteristics of the mixed fillers of the liquid-phase chromatographic column are applied to chromatographic separation under the condition of the mixed mobile phase containing acetonitrile and aqueous solution, wherein the separation application is the chromatographic separation and detection of a sample containing a compound with extremely strong hydrophilism and a compound with extremely strong lipotropism simultaneously; and the separation detection of all the components can be performed once under the condition of equal-grade liquid-phase chromatogram.

A surface-enhanced Raman spectroscopy detection method combined with gel electrophoresis separation technology, comprising: a. using oblique angle deposition technology to prepare silver nanorod arrays; b. combining the gel electrophoresis technology, the substrate prepared in step a and gel electrophoresis Combine the instruments; c. Adjust the voltage and current, so that the mixture to be tested is spread out according to different positions on the substrate; d. Use a surface-enhanced Raman spectrometer to detect the surface-enhanced Raman spectra at different positions on the substrate, and distinguish and judge the mixture. different components. The detection method of the present invention has the advantages of simple detection process, high sensitivity, significant enhancement effect, strong repeatability, and large-scale production; it can effectively utilize the characteristics of high efficiency of gel electrophoresis and high detection of silver nanorods, thereby realizing detection of multiple similar mixtures It can be applied to the separation and detection of a variety of similar complex samples.

The disclosed sensor includes fiber of sensing probe, an insulative fixed frame and two insulative moving frames. An F shaped moving frame and an L shaped moving frame arranged in interlacing are installed on the fixed frame. Optical fiber of sensing probe passing through the interlaced part of the two moving frames is installed on the fixed frame. Three segments of fiber gratings positioned in interlaced and separated part of the two moving frames are made on fiber of sensing probe. First segment of fiber is in use for measuring stress. Two other segments of fiber gratings having same peak wavelength in reflectance spectrum are in use for measuring stress and temperature. Using principle of differential amplification, the invention raises measuring sensitivity, solves crossed sensitivity so as to be able to apply in high voltage and high electromagnetism harsh environment.