47results about How to "Realize separation detection" patented technology

The invention relates to mixed fillers of a liquid-phase chromatographic column and the chromatographic column, and relates to the technical field of analytical chemistry. The mixed fillers of the liquid-phase chromatographic column comprise long-chain alkyl dimethyl silane bonded silica gel and acylamino silane bonded silica gel in a weight ratio of 10:1-1:10, wherein the difference of the mean particle diameter is in a range of between -20 and 20 percent and the difference of the specific weight is in a range of between -20 and 20 percent. In the chromatographic column made by the mixed fillers, the mixed fillers are filled in a column pipe; the outer sides of the two ends of the column pipe are connected with a pipeline through internal thread; the inner sides of the two ends of the column pipe are provided with sieve plates used for preventing the mixed fillers from leaking outwards; one end of the column pipe is provided with a liquid inlet; and a mobile phase enters the chromatographic column from the liquid inlet. The characteristics of the mixed fillers of the liquid-phase chromatographic column are applied to chromatographic separation under the condition of the mixed mobile phase containing acetonitrile and aqueous solution, wherein the separation application is the chromatographic separation and detection of a sample containing a compound with extremely strong hydrophilism and a compound with extremely strong lipotropism simultaneously; and the separation detection of all the components can be performed once under the condition of equal-grade liquid-phase chromatogram.

The invention relates to a detection technique for conductance which is a detecting apparatus and method for separation detecting of capillary electrophoresis, electricity chromatogram and microcylinder liquid phase chromatogram. This invention comprises function signal generator, detecting electrode, electrode fixing device, signal processing circuit, wherein the two detecting electrodes are in telescopic tubular shape and are directly covered on both ends of capillary; and electrode fixing device is composed of two boards with notches in and two detecting electrodes are located in the notches and are connected separately with signal processing circuit; the above components are set in one metal shielding box; the guiding means of capillary is fixed on the side wall of the box; and the guiding wire of detecting electrode is led by the guiding hole and thus is connected with output edge of function signal generator. This invention is of simple structure and high accuracy and good utilization.

A surface-enhanced Raman spectroscopy detection method combined with gel electrophoresis separation technology, comprising: a. using oblique angle deposition technology to prepare silver nanorod arrays; b. combining the gel electrophoresis technology, the substrate prepared in step a and gel electrophoresis Combine the instruments; c. Adjust the voltage and current, so that the mixture to be tested is spread out according to different positions on the substrate; d. Use a surface-enhanced Raman spectrometer to detect the surface-enhanced Raman spectra at different positions on the substrate, and distinguish and judge the mixture. different components. The detection method of the present invention has the advantages of simple detection process, high sensitivity, significant enhancement effect, strong repeatability, and large-scale production; it can effectively utilize the characteristics of high efficiency of gel electrophoresis and high detection of silver nanorods, thereby realizing detection of multiple similar mixtures It can be applied to the separation and detection of a variety of similar complex samples.

The disclosed sensor includes fiber of sensing probe, an insulative fixed frame and two insulative moving frames. An F shaped moving frame and an L shaped moving frame arranged in interlacing are installed on the fixed frame. Optical fiber of sensing probe passing through the interlaced part of the two moving frames is installed on the fixed frame. Three segments of fiber gratings positioned in interlaced and separated part of the two moving frames are made on fiber of sensing probe. First segment of fiber is in use for measuring stress. Two other segments of fiber gratings having same peak wavelength in reflectance spectrum are in use for measuring stress and temperature. Using principle of differential amplification, the invention raises measuring sensitivity, solves crossed sensitivity so as to be able to apply in high voltage and high electromagnetism harsh environment.

A mobile phase for separating phospholipids in high performance liquid chromatography, the composition of the mobile phase of the present invention includes: 10% to 50% of methanol, 50% to 85% of acetonitrile, 0.5% to 3% of fluorinated organic acid, and 0.5 to 3% of water %. The mobile phase of the present invention does not produce any precipitation through long-term use, does not cause damage to the pump system, and does not have any damage to the pump system of the liquid chromatograph; it can be used to detect separated phospholipids in an evaporative light scattering detector, Compared with the UV detector, the detected baseline is stable, easy for quantitative analysis, high sensitivity, and the application of its mobile phase is not subject to any restrictions; compared with the gradient method, the separation and detection can be realized by using a single pump; When proportioning, in addition to effectively separating phospholipids, it can also separate unknown substances that cannot be separated by other mobile phases. When the mobile phase of the present invention is used for isocratic elution, the operation is simple, time-saving, reproducible and easy to repeat.

Provided is a method for detecting nicotine optical isomers in buccal tobacco by using normal-phase liquid chromatography-tandem mass spectrometry. The method is characterized in that firstly, nicotine in the buccal tobacco is extracted, a chiral chromatographic column is adopted to separate the nicotine optical isomers, a post-column high-pressure infusion pump introduces isopropanol and conducts splitting, assay determination is conducted in combination with the tandem mass spectrometry, and therefore the sensitive detection and the separation of S-(-)-nicotine and R-(+)-nicotine are achieved. The method has the advantages that by the adoption of the post-column high-pressure infusion pump for introducing the isopropanol and conducting the splitting, the volume of a non-polar solvent added into a mass spectrometer is reduced, the volume of the polar solvent isopropanol is compensated at the same time, the ionization efficiency is improved, so that requirements of liquid chromatography-tandem mass spectrometry analysis are met, and the sensitivity of the analysis is improved; compared with current methods, the chromatographic separation degree of the two nicotine optical isomers is high, wherein retention time difference between the two nicotine optical isomers is one minute; the analysis time is short, wherein only seventeen minutes are needed.