The invention relates to a positive-phase liquid chromatogram-tandem
mass spectrum detection method for
nicotine optical isomers in tobacco and tobacco products. The positive-phase liquid chromatogram-tandem
mass spectrum detection method is characterized in that
nicotine in tobacco leaves is extracted by a NaOH (
sodium hydroxide) solution and n-
hexane, the
nicotine optical isomers are separated by a chiral
chromatographic column, a post-column
high pressure liquid output pump is used for introducing
isopropyl alcohol and separating flow, and the tandem
mass spectrum is used for analyzing, so as to complete the separation and sensitive detection of S-(-)-nicotine and R-(+)-nicotine in the tobacco and tobacco products. The positive-phase liquid chromatogram-tandem
mass spectrum detection method has the characteristics and beneficial effects that the post-column
high pressure liquid output pump is used for introducing
isopropyl alcohol and separating flow, so that the amount of non-polarity
solvent entering
mass spectrum is reduced, the amount of
isopropyl alcohol of polarity
solvent is compensated, the ionizing efficiency is improved, the requirement of liquid chromatogram-tandem
mass spectrum analysis is met, and the analysis sensitivity is improved; compared with the prior art, the chromatographic resolution of the two types of nicotine optical isomers is high, and the difference of retention times is 1min; the analysis time is short, and is only 17min.