Biogenic amine detection method based on time-resolved dynamic thermal desorption ion mobility spectrometry

A technology of ion mobility spectrometry and detection method, applied in the field of biogenic amine detection based on time-resolved dynamic thermal desorption ion mobility spectrometry, to achieve the effect of improving selectivity and sensitivity and fast analysis speed

Pending Publication Date: 2021-11-02
DALIAN POLYTECHNIC UNIVERSITY
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  • Claims
  • Application Information

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Problems solved by technology

[0005] The invention provides a biogenic amine detection method based on time-resolved dynamic thermal desorption ion mobility spectrometry to solve the problem of simultaneous and rapid detection of various biogenic amines

Method used

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  • Biogenic amine detection method based on time-resolved dynamic thermal desorption ion mobility spectrometry
  • Biogenic amine detection method based on time-resolved dynamic thermal desorption ion mobility spectrometry
  • Biogenic amine detection method based on time-resolved dynamic thermal desorption ion mobility spectrometry

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Embodiment 1

[0041] In the positive ion mode, toluene is used as the reagent molecule, the thermal desorption temperature is 120°C, the transfer tube temperature is 100°C, the carrier gas flow rate is 200mL / min, the drift gas flow rate is 300mL / min, and the voltage difference between the two metal rings is 178V, the electric field distribution on the transfer tube is 250V / cm, and the ion mobility spectra of putrescine, cadaverine, spermine, spermidine, phenylethylamine and histamine with different reduced mobility are respectively obtained, as shown in figure 1As shown, it can be seen from the figure that toluene mainly forms a reduced mobility of 2.07cm 2 V -1 the s -1 The reagent ion of the reagent, the reagent ion reacts with the seven biogenic amines to form corresponding characteristic product ions respectively. Among them, the reduced mobility of putrescine formation is 1.94cm 2 V -1 the s -1 The product ion of cadaverine formation reduced mobility 1.82cm 2 V -1 the s -1 The ...

Embodiment 2

[0043] In the positive ion mode, acetone is used as the reagent molecule, the thermal desorption temperature is 120°C, the transfer tube temperature is 120°C, the carrier gas flow rate is 200mL / min, the drift gas flow rate is 300mL / min, and the voltage difference between the two metal rings is 178V, the electric field distribution on the transfer tube is 250V / cm, and the ion mobility spectra of putrescine, cadaverine, spermine, spermidine, phenylethylamine and histamine with different reduced mobility are respectively obtained, as shown in figure 2 As shown, it can be seen from the figure that acetone mainly forms a reduced mobility of 1.84cm 2 V -1 the s -1 The reagent ion of the reagent, the reagent ion reacts with the seven biogenic amines to form corresponding characteristic product ions respectively. Among them, the reduced mobility of putrescine formation is 1.94cm 2 V -1 the s -1 and 1.71cm 2 V -1 the s -1 Two product ions; cadaverine formation reduced mobility...

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Abstract

The invention provides a biogenic amine detection method based on time-resolved dynamic thermal desorption ion mobility spectrometry, which adopts a thermal desorption sampler to realize time-resolved sample introduction of different biogenic amines based on different thermal desorption characteristics of various biogenic amines; meanwhile, reagent molecule-assisted positive ion photoionization ion mobility spectrometry is adopted for detecting the biogenic amines, and the detection sensitivity and recognition accuracy of the biogenic amines are improved through reagent molecule screening, so that the simultaneous rapid detection of various biogenic amines is realized. The method provided by the invention can realize simultaneous accurate quantification of multiple mixtures, and has the advantages of high sensitivity, fast detection speed and portable instrument.

Description

technical field [0001] The invention relates to the technical field of food safety, more specifically, to a biogenic amine detection method based on time-resolved dynamic thermal desorption ion mobility spectrometry, which realizes simultaneous and rapid detection of different biogenic amines. Background technique [0002] After the seafood is caught, if it is not processed and preserved in time, it is very prone to spoilage and deterioration under the action of endogenous and microorganisms. The spoilage will lead to the decarboxylation of free amino acids in seafood to produce biogenic amines. Biogenic amines are a class of nitrogen-containing low-molecular-weight basic compounds with biological activity. Common ones include histamine, putrescine, cadaverine, tryptamine, phenylethylamine, spermine, and spermidine. When biogenic amines accumulate in the human body to a certain extent, they will be toxic to the human body, causing allergic reactions such as headache, nausea,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/622
CPCG01N27/622
Inventor 程沙沙董雪林蓉谭明乾刘辰玥刘芯怡
Owner DALIAN POLYTECHNIC UNIVERSITY
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