Method for detecting cetilistat in weight-losing health-care product

A new technology of lisstat and health care products, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effect of simple pretreatment process, broad application prospects, and short time consumption

Pending Publication Date: 2022-07-08
GUANGZHOU INSPECTION TESTING & CERTIFICATION GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The addition of lipase inhibitors is prohibited in weight loss health products, but the method for detecting new lixistat in weight loss health products has not yet been reported

Method used

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  • Method for detecting cetilistat in weight-losing health-care product
  • Method for detecting cetilistat in weight-losing health-care product

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specific Embodiment

[0119] The embodiments of the present invention will be described in detail below with reference to the examples. It should be understood that these examples are only used to illustrate the present invention and not to limit the scope of the present invention. In the following examples, the experimental methods that do not indicate specific conditions, preferably refer to the guidelines given in the present invention, and can also follow the experimental manuals or conventional conditions in the field, and can also follow the conditions suggested by the manufacturer, or refer to the existing conditions in the art. known experimental methods.

[0120] In the following specific examples, the measurement parameters related to raw material components may have slight deviations within the range of weighing accuracy unless otherwise specified. Involves temperature and time parameters, allowing acceptable deviations from instrument test accuracy or operational accuracy.

Embodiment 1

[0121] Embodiment 1, carry out recovery test to sugar-free black coffee beverage sample

[0122] 1 Reagents and Materials

[0123] Unless otherwise specified, the reagents used are all analytically pure; the water is first-grade water in accordance with GB / T 6682.

[0124] Acetonitrile: chromatographically pure.

[0125] Methanol: chromatographically pure.

[0126] Ammonium acetate: analytical grade.

[0127] Orlistat standard (cas number: 96829-58-2, molecular formula: C 29 H 53 NO 5 ): purity ≥95%.

[0128] Rimonabant standard (cas number: 168273-06-1, molecular formula: C 22 H 21 C l3 N 4 O): Purity ≥ 95%.

[0129] New Lilistat (cas number: 282526-98-1, molecular formula: C 25 H 39 NO 3 ): purity ≥95%.

[0130] Information about orlistat, rimonabant, and new orlistat is as follows:

[0131] Table 1. Chinese name, English name, CAS number, molecular formula, relative molecular mass and structural formula of three compounds

[0132]

[0133] 2 Preparation ...

Embodiment 2

[0185] Embodiment 2, carry out standard addition recovery test to compressed candy sample

[0186] 1 Reagents and Materials

[0187] Refer to Example 1.

[0188] 2 Preparation of reference solution

[0189] Refer to Example 1.

[0190] 3 Pretreatment of samples

[0191] ①Accurately weigh 5g (accurate to 0.01g) of the sample and place it in a beaker, add 10mL of 40°C-60°C warm water to fully dissolve the sample, and add a certain amount of orlistat, rimonabant and new orlistat (each The drug corresponds to 3 levels of addition, which are 0.05mg / kg, 0.15mg / kg, 0.50mg / kg) for standard addition recovery experiments;

[0192] ② Transfer the sample to a 50 mL volumetric flask, add 5 mL of methanol to dilute the sample, and use methanol to dilute the sample;

[0193] ③ Put the sample in the ultrasonic equipment for 30min to make the target fully dispersed in the target phase;

[0194] ④Place the ultrasonicated sample in a refrigerated centrifuge, centrifuge at 4000 rpm for 2 mi...

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Abstract

The invention relates to a method for detecting cetilistat in a weight-losing health-care product. The method comprises the following steps: providing a reference substance solution, wherein a reference substance in the reference substance solution contains cetilistat; taking a weight-losing health-care product sample, extracting with methanol, collecting an extracting solution, and preparing a test solution; and detecting the reference solution and the test solution by adopting a high performance liquid chromatography-tandem mass spectrometry method, and determining whether the weight-losing health-care product sample contains the cetilistat or/and the content of the cetilistat according to a detection result. According to the method disclosed by the invention, the separation and detection of the cetilistat in the weight-losing health-care product are realized by adopting a proper extraction solvent and matching with proper detection conditions, the technical blank of the detection of the cetilistat in the weight-losing health-care product is filled, and a theoretical basis is laid for applying a high performance liquid chromatography-tandem mass spectrometry technology to the detection of the cetilistat in the weight-losing health-care product.

Description

technical field [0001] The invention relates to the technical field of health-care product quality detection, in particular to a method for detecting new lyostat in weight-loss health-care products. Background technique [0002] New Lilistat (Cetilistat / ATL-962) is a new type of gastrointestinal lipase inhibitor, which can inhibit the activity of Pancreatic lipase, which can be used for the treatment of dyslipidemia and type Ⅱ diabetes mellitus, as well as after exercise. BMI still 25kg / m after diet therapy 2 obesity above. The advantage of new Lilistat is that it neither acts on the nervous system, does not affect the activity of other enzymes in the gastrointestinal tract, nor is absorbed into the blood, and does not suppress appetite. [0003] It is forbidden to add lipase inhibitors in weight loss health care products, however, the method for detecting new Lilistat in weight loss health care products has not yet been reported. Therefore, how to realize the detection o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/72G01N30/86
CPCG01N30/02G01N30/06G01N30/34G01N30/7266G01N30/8675G01N2030/047
Inventor 黄嘉乐何敏恒周朗君陈扬罗伟倩段雪菲黄芷仟
Owner GUANGZHOU INSPECTION TESTING & CERTIFICATION GRP CO LTD
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