Method for rapidly and simultaneously determining contents of crocin-1, crocin-2, crocin-3 and crocin-4

A fast technology of crocin, applied in the field of analytical chemistry, can solve the problems of difficulty in popularization, high detection cost, high price, etc., and achieves the effects of strong practicability, reduced economic cost, and easy operation.

Active Publication Date: 2022-04-12
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Liquid chromatography-mass spectrometry can realize the simultaneous separation and identification of the components of the crocin mixture, but this method requires the use of expensive mass spectrometers, and the detection cost is high, making it difficult to popularize
[0010] In summary, none of the above detection methods can achieve the simultaneous separation of crocin-1, crocin-2, crocin-3 and crocin-4 under one experimental condition. There is also no report in the technology that there is a method for simultaneously determining the content of crocin-1, crocin-2, crocin-3 and crocin-4

Method used

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  • Method for rapidly and simultaneously determining contents of crocin-1, crocin-2, crocin-3 and crocin-4
  • Method for rapidly and simultaneously determining contents of crocin-1, crocin-2, crocin-3 and crocin-4
  • Method for rapidly and simultaneously determining contents of crocin-1, crocin-2, crocin-3 and crocin-4

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] A method for rapid and simultaneous determination of crocin-1, crocin-2, crocin-3 and crocin-4, using ultra-high performance liquid chromatography (Agilent 1290 Infinity II), Crocin-1 (Nanjing Jingzhu Biotechnology Co., Ltd.), saffron-2 (Nanjing Jingzhu Biotechnology Co., Ltd.), saffron-3 (Nanjing Jingzhu Biotechnology Co., Ltd. ), saffron The standard substance of anthocyanin-4 (Nanjing Jingzhu Biotechnology Co., Ltd.) was used as a control to detect the content of crocin components in dried gardenia fruits. Specifically include the following steps:

[0057] 1) Preparation of the reference substance solution: take appropriate amounts of crocin-1, crocin-2, crocin-3 and crocin-4 respectively, weigh them accurately, place them in 25ml volumetric flasks, and Use 50% methanol to prepare them into stock solutions with mass concentrations of 100, 20, 20, and 20ug / ml; draw 0.60, 2.40, 2.40, and 2.40ml of the stock solutions above into the same 10ml volumetric flask, and add ...

Embodiment 2

[0066] In this embodiment, the gardenia yellow pigment is used as a sample to prepare a test sample solution, and the effects of crocin-1, crocin-2, crocin-3, and crocin-4 in gardenia yellow The content is measured, and the difference between its assay method and Example 1 is only: the preparation steps of the test solution are: accurately weigh 1 mg of gardenia yellow pigment in a 10ml centrifuge tube, add 50% methanol to constant volume, and pass through 0.22um Organic system microporous membrane filtration, obtain 100ug / ml need testing solution. After determination, the contents of crocin-1, crocin-2, crocin-3 and crocin-4 in gardenia yellow powder were 351.56, 20.84, 42.51 and 3.37 mg / g, respectively.

Embodiment 3

[0067] Embodiment 3 methodological investigation

[0068] In the present embodiment, gardenia yellow pigment is used as a sample to prepare a test solution, and the precision, stability, repeatability, and sample recovery rate of the assay method in the present invention are investigated, and gardenia yellow pigment is used as a sample for testing. The preparation steps of the product solution are: accurately weigh 1 mg of gardenia yellow pigment in a 10ml centrifuge tube, add 50% methanol to constant volume, and filter through a 0.22um organic microporous membrane to obtain a 100ug / ml test product solution. Adopt step 3) in the embodiment 1) described chromatographic condition to carry out chromatographic detection to this need testing solution, its chromatogram is as follows image 3 shown.

[0069] (1) Inspection of precision

[0070] According to the chromatographic conditions described in step 3) in Example 1, the samples were injected continuously 6 times within 1 day ...

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Abstract

The invention discloses a method for rapidly and simultaneously determining the content of crocin-1, crocin-2, crocin-3 and crocin-4. The method comprises the following steps: 1) preparing a reference substance solution; (2) preparing a test solution; 3) loading the sample on a chromatographic column, and using Agilent Poroshell 120 EC-C18 (2.7 [mu] m, 3 * 150 mm) as the chromatographic column; acetonitrile is used as a mobile phase A, 0.1% acetic acid water is used as a mobile phase B, and gradient elution is performed: 24%-25.5% of A and 76%-74.5% of B are performed for 0-9 min; in 9-15 min, 25.5%-30% of A is adopted, and 74.5%-70% of B is adopted; 30%-35% of A and 70%-65% of B are added in 15-18 min; 18-26 min, 35%-40% of A and 65%-60% of B are added; in 26-28 min, 40%-24% of A and 60%-76% of B are added; the flow rate is 0.4 ml / min; the detection wavelength of the ultraviolet detector is 440 nm; the column temperature is 30 DEG C; the sample size is 5 microliters; according to the method, simultaneous determination of crocin-1, crocin-2, crocin-3 and crocin-4 is achieved, the method is simple and high in accuracy, the detection time is greatly shortened, and the separation and identification efficiency of different components of saffron is improved.

Description

technical field [0001] The invention relates to the technical field of analytical chemistry, in particular to a method for quickly and simultaneously measuring the contents of crocin-1, crocin-2, crocin-3 and crocin-4. Background technique [0002] Crocetin is a series of ester compounds formed by carotenoid crocetin and sugar, which only exists in saffron and gardenia. It has unique pharmacological activity and economic value. Its main structure is crocin glycoside , including crocin-1, crocin-2, crocin-3, crocin-4 and other ingredients, the molecular structure is: [0003] [0004] As the amount of glucose in the structure increases, the polarity of each crocin component increases correspondingly, the stability decreases, and the separation difficulty is higher. Since the component with the highest content in crocin is crocin-1, which accounts for about 70% to 80%, the research on pharmacological effects related to crocin is mainly based on this component. Therefore, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/34G01N30/06G01N30/02
CPCY02A50/30
Inventor 王建武李天娇匡梦雅邝鼎
Owner CENT SOUTH UNIV
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