777 results about "Crocin" patented technology

The invention relates to unique compositions containing enriched and purified natural crocin and / or crocetin for prevention and / or treatment of cancers and other conditions and diseases. Compositions comprise mainly enriched or purified natural crocin or crocetin or combination of both and possible other active phytochemicals. A composition is used as functional food, drink, dietary supplement, or therapeutic dosage to a human orally or through other appropriate way (parenteral, percutaneous, rectal, mucosal, intranasal or topical administration). A method of natural crocin and crocetin enriching and purification is revealed.

The invention relates to unique compositions containing enriched and purified natural crocin and / or crocetin for prevention and / or treatment of cancers and other conditions and diseases. Compositions comprise mainly enriched or purified natural crocin or crocetin or combination of both and possible other active phytochemicals. A composition is used as functional food, drink, dietary supplement, or therapeutic dosage to a human orally or through other appropriate way (parenteral, percutaneous, rectal, mucosal, intranasal or topical administration). A method of natural crocin and crocetin enriching and purification is revealed.

The invention discloses a preparation method of high pure crocin and geniposide. The preparation method comprises following main steps: selecting raw materials of gardenia ellis plants, extracting with water or an alcohol-water mixed solution, merging the extracting solutions, condensing, filtering, subjecting the obtained extracting solution to an adsorption treatment by macroporous resin, then performing gradient elution with a ethanol-water mixed solution, obtaining geniposide and gardenia yellow refined extracts; subjecting the geniposide refined extract to a recrystallization treatment with ethyl acetate so as to obtain the geniposide product; subjecting the gardenia yellow refined extract to go through column chromatography, which has been pressurized and stuffed with modified silica gel, to separate, and obtaining the high pure crocin product (color value larger than 600) and a chlorogenic acid component. The products have the advantages of high purity, simple technology, strong operability and convenience for automation, comprehensive utilization of the plant resources is achieved, the solvent is convenient to recycle and reuse, and the preparation method is easy to apply to the industrial amplification.

The present invention relates to a composition derived from the hydrolysate of plant containing crocin or the derivatives thereof. The composition includes a significant amount of crocetin monoester. The present invention also provides a method to hydrolyze crocin existing in a plant extract, and further relates to the use.

The invention relates to a preparation method of the crocetin extracted from the gardenia, which is characterized in that after the gardenia is crushed and extracted by ultrasonic enhancement in distilled water or ethanol solution, the extractant is filtered and concentrated; the glycoside substance of the gardenia is separated via the macroporous resin and the crocin eluent is concentrated and dealt with acid hydrolysis or alkaline hydrolysis (alkaline hydrolysis solution should be adjusted to pH of acidity.), centrifugal, rinse and sedimentation to get the crude product of the crocetin; after purifying the crude product of the crocetin further, the cis crocetin and the trans crocetin are separated to acquire trans crocetin of high purity which facilitates structure modification, forms a plurality of crocetin derivatives featured by different water soluble and drug efficacy, providing material for related preparation R and D and application. The preparation method of the crocetin extracted from the gardenia has the advantages of having convenient and fast method, low cost and establishing a new channel for deep processing of gardenia products.

The invention relates to a traditional Chinese medicine fertilizer for flowers and a preparation method thereof, and belongs to the technical field of fertilizers. The traditional Chinese medicine fertilizer is characterized by being prepared from the following raw materials in parts by weight: 12-22 parts of concha arcae, 15-18 parts of trogopterus dung, 8-12 parts of camphor, 15-18 parts of pine needles, 20-22 parts of plantain herb, 18-22 parts of honeysuckle, 8-12 parts of saffron crocus, 15-17 parts of poria cocos, 18-20 parts of pseudo-ginseng, 12-15 parts of fructus chebulae, 10-14 parts of calculus bovis and 10-14 parts of liquorice. According to the traditional Chinese medicine fertilizer, various traditional Chinese medicine materials are effectively combined, so that a large amount of microelements such as calcium, iron, sulfur, magnesium, boron, manganese, copper and zinc are provided, the plant growth can be promoted, pathogenic bacteria can be inhibited, the attack of the pathogenic bacteria can be reduced, a certain pest killing effect can be achieved, the use of chemical fertilizers and pesticides is reduced, and the environment is favorably protected.

The invention discloses a preparation method of a rice mate rich in crocin and geniposide, particularly relates to a preparation method of a rice mate rich in crocin and geniposide by utilization of a subcritical extraction technology, and belongs to the field of medicinal food and healthcare food. The preparation method includes three steps, namely, degreasing, desolventizing and cooking. More than 99.9% of liposoluble constituents in materials can be extracted by the subcritical extraction technology (with the oil content of dregs after the degreasing step being lower than 0.1%). The product can be directly used or be further processed as a golden rice mate to prepare jasmine golden rice, and the like. In addition, the biological activity of functional components in the extract is guaranteed and the product is liable to store. Effective components which are the crocin and the geniposide are preserved in the dregs can be further widely applied. Comprehensive utilization of nutritions in the "dregs" after fat is extracted is guaranteed. The method has a wide application scope.

The invention relates to a method capable of synchronously separating and purifying a crocin I monomer and a crocin II monomer, and belongs to the technical field of separation and purification for active ingredients of Chinese traditional medicines. The method comprises the following steps: taking dried stigmas of Crocus sativus as raw materials, performing 90-95% ethanol extraction, concentration, filter, efficient preparative liquid chromatography separation, product recovery and sephadex gel column chromatography to obtain the crocin I monomer and the crocin II monomer. By virtue of the optimum technique and parameter conditions, the content of the crocin I monomer and the crocin II monomer in the product is up to 99% above respectively; the method is stable in the whole technical process, convenient to operate, high in separation efficiency and low in cost, and can separate out and prepare the crocin I monomer and the crocin II monomer with high purity and large scale.

The invention relates to a process for extracting alpha-crocin from fructus gardeniae, which comprises the following steps of degreasing, aqueous extraction, tannin removal, resin adsorption, recrystallization and the like, and particularly comprises a method of degreasing by a No.4 solvent, tannin removal by polyamide resin and the recrystallization of hot water and propylene glycol. The production process has the characteristics of simplicity, convenience and low cost.

The present invention relates to a method for treating abnormal polyglutamine-mediated diseases comprising: administering a pharmaceutical composition comprising one or more selected from the group consisting of a Gardenia jasminoides extract, geniposide and crocin.

The invention relates to a method used for preparing high-purity gardenoside and crocin from gardenia jasminoides ellis, and belongs to the technical field of food and medicine. The method comprises following steps: a, gardenia jasminoides ellis is collected, and is extracted at a temperature of 40 to 70 DEG C, an obtained extracted solution is cooled to room temperature, active carbon or diatomite is added, and an obtained mixture is filtered and concentrated so as to obtain an extractum; b, the extractum is dissolved in water, pH value is adjusted to 7.8 to 9, an obtained solution is processes by using a MCI gel chromatographic column, is allowed to stand, and is subjected to elution with water, an obtained mixed material is subjected to elution with an ethanol solution with a mass percent concentration of 5 to 30% so as to obtain an eluent containing gardenoside, and an obtained product is subjected to elution with an ethanol solution with a mass percent concentration of 60 to 90% so as to obtain an eluent containing crocin; c, the eluent containing gardenoside is concentrated, is dissolved in ethyl acetate or absolute ethyl alcohol, and is subjected to crystallization so as to obtain high-purity gardenoside; the eluent containing crocin is concentrated and dried so as to obtain high-purity crocin. Beneficial effect of the method is that: purity of gardenoside and purity of crocin are both high.

The invention relates to a saffron crocus planting method. The saffron crocus planting method comprises the steps of outdoor corm breeding: saffron crocus corm can be transplanted in an outdoor field in last November and can be removed from soil in next May; indoor bloom cultivation: from the middle of May to mid-to-end of June, seedballs are in a complete dormancy period, and the room temperature needs to be up to 30 DEG C and needs to be maintained for more than 20 days; from the end of June to the last ten days of July, the room temperature needs to be maintained in 23-28 DEG C, the relative humidity is 80%, the indoor environment is in sun shading, and the illumination is avoided; from August to the middle of September, the room temperature needs to be maintained in 23-27 DEG C, the relative humidity is 75%-85%, the indoor environment is in sun shading, and the illumination is avoided; from the end of the middle of September to the last ten days of October, white parts are presented by main sprouts of the corm, the room temperature is maintained in 20-25 DEG C, and the relative humidity is 80%; from the end of October to the middle of November, the room temperature is 15-18 DEG C, the humidity is 75%, and the indoor environment is not in sun shading any more. According to the saffron crocus planting method disclosed by the invention, the yield is high, the quality is good, and the economic benefit is good.

The invention discloses a method for preparing crocetin and crocin, which comprises the following steps: preparing a gardenia fruit extracting solution with ethanol under the conditions of ultrasonic waves, and concentrating the extracting solution to obtain an extract; and dissolving the extract in a polyethylene glycol aqueous solution, and selectively extracting with solvent to obtain crocetin and crocin. The invention does not need the chromatographic column separation process, has the advantages of simple operational method, and high product yield and purity, and is suitable for industrial production.

A Chinese medicine in the form of honeyed pills for treating chronic hepatism and hepatic fibrosis is prepared from 13 Chinese-medicinal materials including saffron, red sage root, astragalus root, Chinese angelica root, etc through cleaning, pulverizing, sieving, proportionally mixing, adding honey, pilling and Co-60 sterilizing. Its advantage is high curative effect up to 94%.

The invention which discloses a quality detection method for a medicinal composition for treating the hepatopathy belongs to the technical field of medicines. According to the invention, present Ganchang capsule quality standards are correspondingly improved, safflower and Herpetospermum pedunculosum discriminating methods are added on the basis of original standards, methods for determining contents of effective components crocin-I and crocin-II of Crocus sativus in the preparation, an effective component hydroxysafflor yellow A of safflower in the preparation, an effective component bilirubin of artificial calculus bovis in the preparation, and an effective component swertiamarin of Herba Swertiae Chirayitae in the preparation, an effective component eugenol of clove in the preparation are added, so the safe quality, the uniformity, the stability and the controllable quality of the preparation are further ensured, thereby clinical curative effects of the preparation and the body health of general patients are ensured.

The invention discloses a quality detection method of a Zhenlong brain-refreshment preparation. The method provided by the invention comprises a discrimination method of piper longum, banksia rose, Chinese cassia tree, gallic acid, cardamom, clove, pearl, cholic acid, stigma croci and cinnamaldehyde as well as a discrimination method of piperine in piper longum, cholic acid in cow-bezoar, cinnamaldehyde in Chinese cassia tree, and crocin-I and crocin-II in stigma crociand. Through a test research, by the discrimination methods in the quality detection method of the test article chromatograph,spots with the same color are shown on the corresponding position of control article chromatograph without the interference of negative control. The method is convenient and feasible with strong character. The content detection method provided by the invention is used to detect the contents of piperine, cholic acid, crocin-I and crocin-II in the Zhenlong brain-refreshment preparation. The result shows that the method has good linear relation, high accuracy of a recovery test and good repeatability and stability, and can be used to accurately and rigorously control the quality of the Zhenlong brain-refreshment preparation.

Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to express a zeaxanthin cleavage dioxygenase alone or in combination with recombinant genes encoding UDP-glycosyltransferases (UGTs). Such microorganisms, plants, or plant cells can produce compounds from saffron such as crocetin, crocetin dialdehyde, crocin, or picrocrocin.

The invention discloses a gardenia fruit oil and a preparation method thereof. The method is characterized in that subcritical extraction is carried out on full gardenia fruits, shelled gardenia fruits, or gardenia residues obtained after pigment extraction through a water percolating technique to obtain gardenia fruit oil. The gardenia fruit oil contains carotenoids characterized by natural crocin; the content of total carotenoids is about 50-250mg / kg by determining through spectrophotometry (440-460nm); and the total carotenoids contain crocin, xanthin, zeaxanthin and other oxygen-containing carotenoids. The gardenia fruit oil contains carotenoids characterized by natural crocin, so the gardenia fruit oil can be developed to form many healthy products with high health values and added values. The subcritical fluid preparation method has the potential to become one of the ways to solve the lack of edible oil in China.

The present invention particularly relates to a nature crocin extraction separation method and preparation for a crocin blood fat reduction drug. The extraction separation method comprises: carrying out cleaning and impurity removing on dried cape jasmi fruits, extracting with deionized water, filtering to removing residue, carrying out online membrane separation and concentration fusion and macroporous absorption resin chromatography purification, and finally carrying out low-temperature spray drying to prepare the crocin. According to the present invention, the key of the invention is to create the extraction agent, and the water containing a certain auxiliary solvent is adopted to extract the crocin, wherein the extraction rate can be more than 95%; the online membrane separation and concentration fusion and the macroporous absorption resin chromatography purification are integrated; the high purity crocin production method has a characteristic of low cost and is suitable for industrial expansion production; and the compounded pharmaceutical preparation has effects of significant blood fat reduction, no side effect, effective cardiovascular and cerebrovascular related disease prevention, and blood circulation promotion, and is the preferred health care drug for crowds with high blood pressure, high blood fat and high blood sugar.

The invention discloses a fingerprint atlas analyzing method; which is characterized by the following: adding buffer in the inversed-phase chromatogram system for west carthamin; possessing stable chemical property for west carthamin-1, west carthamin-2, west carthamin-4 in the flow phase; displaying precise quantitative property and stable reserving time for west carthamin-3; satisfying the request of fingerprint atlas analysis.

The invention provides a purification and separation technology of crocin in a gardenia extract, namely a technology for adsorbing, purifying and separating crocin components by utilizing a foam separator and macroporous resin columns of different polarities. The technology comprises the following operation steps: adding an aqueous solution of the gardenia extract into the foam separator according to the differences in hydrophilcities and polarities of different components in crocin, adding ethyl acetate for extraction, concentrating the subnatant into dry paste, fully dissolving the dry paste in 15% ethanol, introducing the dissolution solution into four macroporous resins for adsorption, and performing gradient elute by using ethanol with different concentrations, thus purifying and separating crocin-1, crocin-2, crocin-3 and crocin-4. The selected raw materials are low in cost and readily available, the separation and purification process is simple and convenient to operate, each component of the purified crocin is high in purity and low in impurity content, and the quality performance index can reach the industrial standards of medicines, foods and cosmetics.

The invention belongs to the field of cosmetics and particular relates to hirudin skin-whitening moisture-keeping conditioning cream and a production process thereof. The conditioning cream is prepared by oil phase raw materials such as squalane, polyoxyethylene stearyl alcohol ether, jojoba oil, white oil, ferulic acid derivative, cetostearyl alcohol and evening primrose oil, and water phase raw materials such as hirudin, oat polypeptide, a saffron crocus extracting solution, collagen polypeptide, caffeine derivative and an epidermal growth factor. The production process comprises the steps of heating, sterilization, stirring, dissolving, emulsifying, homogenizing, cooling, discharging, sampling, detection, filling, and packaging to obtain the conditioning cream. The conditioning cream can permeate into a corium layer of skin autonomously and directly, can be easily absorbed by the skin, can solve the problems such as the lusterless skin caused by pore blockage, has a better skin-whitening, moisture-keeping and skin-softening effect, and can promote regeneration of the skin and improve the skin quality; as multiple skin-whitening, moisture-keeping and skin-tone-improving raw materials are added, the conditioning cream can improve blood circulation and the skin elasticity, and has very good skin-whitening, moisture-keeping and skin-softening effects; and the production process is simple and easy to operate.

The invention relates to the technical fields of separation and purification of medicines and especially relates to an extraction method of crocin I. The extraction method includes following steps: 1) performing alcohol-extraction to medicinal parts of a plant containing the crocin I to obtain an extract liquid; 2) performing adsorption and separation to the extract liquid to prepare a first intermediate; 3) purifying the first intermediate by a medium-pressure chromatographic system to obtain a second intermediate, wherein a chromatographic agent in the medium-pressure chromatographic system is a polystyrene-type high-molecular macroporous polymer material and a mobile phase is an ethanol aqueous solution; and 4) crystallizing the second intermediate to obtain the crocin I. The extraction method is used for preparing a crocin I crystal being high in purity and color value, of which the purity can reach more than 95% and the color value can reach more than 950.

The present invention relates to a carotenoid cleavage dioxygenase CCD4a involved in the synthesis of gardenia jasminoides ellis crocin, an encoding gene and functions thereof, belongs to the technical field of gene engineering, and discloses the open reading frame sequence of a carotenoid cleavage dioxygenase CCD4a gene and the amino acid sequence encoded by the carotenoid cleavage dioxygenase CCD4a gene. According to the present invention, the CCD4a gene is screened based on transcriptomics, the functions of CCD4a are verified in vitro by using zeaxanthin as the substrate and constructing the pET28a-CCD4a prokaryotic expression vector, and the results prove that CCD4a has the functions of carotenoid cleavage dioxygenase so as to establish the foundation for the analysis and biosynthetic research of the crocin source pathway.

The invention relates to a traditional Chinese medicine composition for removing freckles and nourishing skin and a preparation method for the same, wherein the traditional Chinese medicine composition contains traditional Chinese medicines in the following parts by weight: 10-100 parts of radix bupleuri, 10-100 parts of bighead atractylodes rhizome, 10-100 parts of ginger, 10-100 parts of salviamiltiorrhiza, 10-100 parts of peach kernels, 10-100 parts of artemisia apiacea, 10-100 parts of ligusticum wallichii, 20-80 parts of angelica sinensis, 20-80 parts of radix paeoniae alba, 20-80 partsof fried gardenias, 20-80 parts of rhizoma cyperi, 10-70 parts of moutan bark, 10-70 parts of mint and 1-10 parts of saffron crocus. The traditional Chinese medicine composition provided by the invention can be used for treating facial chloasma and chromatosis.

The invention discloses a rotation planting technology for saffron crocus with rice, peony and green vegetables. According to the planting technology, saffron crocus is in alternate rotation with rice in a field, saffron crocus is in rotation with peonies and green vegetables in a greenhouse, and the crops for rotation are linked in the mode that in the first season, rice is sown in the field in the middle ten days of May, and harvesting is conducted in the last ten days of November; in the second season, saffron crocus is sown in the first ten days in December, bulbs are harvested in the first ten days of May in the next year and put on shelves in July in the greenhouse, flowers are harvested in the last ten days in November, and the shelves are removed; in the third season, peonies are put into pots in the first ten days of December, enter the greenhouse for flower forcing and are harvested around the spring festival; in the fourth season, green vegetable are planted in the greenhouse from the middle ten days of February to the last ten days of June for multiple batches. The rotation planting technology comprises the specific steps that firstly, rice is planted; secondly, saffron crocus is planted; thirdly, peonies are planted; fourthly, green vegetables are planted. By means of the rotation planting technology, the problem of soil impediment caused by continuous cropping of rice, saffron crocus and vegetable, efficient coupling of water and fertilizer is achieved, and plant diseases and insect pests spread through soil are relieved.

The invention provides Tibetan medicine medicated bath for treating psoriasis. The Tibetan medicine medicated bath is prepared by preparing ephedra saxatilis, shug-pa, artemisia sieversiana, german tamarisk, rhododendron anthopogonoide, mirabilis jalapa, sealwort, radix asparagi, caltrop, sphallerocarpus gracilis, semen cassia, ambrette, frankincense, sulphur, orpiment, saffron crocus, ginger and rheum officinale, partially fermenting and decocting the components. The Tibetan medicine medicated bath provided by the invention can quickly and directly act on the lesions, perfect the vitality of skin tissues, enhance the metabolism of skin, promote the blood circulation of a human body, adjust the endocrine, discharge toxins in vivo, kill dermatophyte, enhance the immune function and ensure an obvious treatment effect; and the treatment process is comfortable and convenient and is free of toxic or side effect.

The invention relates to the medicine field, and discloses a preparation method of crocetin. Extraction is carried out by adopting diluted alkali, extraction condensation and hydrolysis are combined together, and crocin is hydrolyzed and converted into crocetin during extraction and concentration. The preparation method comprises the steps of: crushing raw materials, carrying out conversion extraction by using a diluted alcoholic solution with pH of 10-14, regulating the pH to be 7-9 by using an extracting solution, condensing by decompression, filtering or centrifuging to remove sediment impurities, regulating the pH to be 2-5, filtering or centrifuging to collect red sediments and obtain a crocetin crude product with crocetin content higher than 50 percent, and recrystallizing and purifying the crocetin crude product to obtain the high-purity trans-crocetin with the crocetin content higher than 95 percent. The trans-crocetin can be used for preparing a medicament for treating fattyliver and has wide application prospect in prevention and control of tumors and other diseases.

The invention relates to a medicine for gynecoiatry and health care and a preparation method thereof. The medicine is made of the formulation based on the weight percentage: 2-12% of rhizoma atractylodis, 3-15% of bletilla striata, 2-10% of angelica dahurica, 3-12% of platycodon root, 2-10% of phyllanthus urinaria, 2-10% of saffron, 4-13% of tuckahoe, 4-10% of asiatic plantain seed, 2-10% of radix paeoniae alba, 2-10% of baikal skullcap root, 2-10% of rhizoma coptidis, 3-10% of gentianella, 3-12% of achyranthes, 3-10% of barbed skullcap herb, 3-8% of hibiscus, 2-10% of dictamnus dasycarpus, 2-15% of motherwort, 3-8% of forsythia, 1-10% of angelica, 2-8% of common cnidium fruit, 3-7% of cistanche salsa, 2-8% of salvia chinensis, 1-6% of salvia miltiorrhiza, 1-4% of nut grass and 2-8% of radix curcumae; the total content of the twenty-five drugs is 100%. The medicine has special effects on expulsion of toxin, antiphlogosis and sterilization of gynae, has the functions of stanching, astringing and healing wound, and has the remarkable advantages of no side effect and rapid treatment.

The invention relates to unique compositions containing enriched and purified natural crocin and / or crocetin for prevention and / or treatment of cancers and other conditions and diseases. Compositions comprise mainly enriched or purified natural crocin or crocetin or combination of both and possible other active phytochemicals. A composition is used as functional food, drink, dietary supplement, or therapeutic dosage to a human orally or through other appropriate way (parenteral, percutaneous, rectal, mucosal, intranasal or topical administration). A method of natural crocin and crocetin enriching and purification is revealed.