87 results about "Crocetin" patented technology

The invention relates to unique compositions containing enriched and purified natural crocin and / or crocetin for prevention and / or treatment of cancers and other conditions and diseases. Compositions comprise mainly enriched or purified natural crocin or crocetin or combination of both and possible other active phytochemicals. A composition is used as functional food, drink, dietary supplement, or therapeutic dosage to a human orally or through other appropriate way (parenteral, percutaneous, rectal, mucosal, intranasal or topical administration). A method of natural crocin and crocetin enriching and purification is revealed.

The invention relates to unique compositions containing enriched and purified natural crocin and / or crocetin for prevention and / or treatment of cancers and other conditions and diseases. Compositions comprise mainly enriched or purified natural crocin or crocetin or combination of both and possible other active phytochemicals. A composition is used as functional food, drink, dietary supplement, or therapeutic dosage to a human orally or through other appropriate way (parenteral, percutaneous, rectal, mucosal, intranasal or topical administration). A method of natural crocin and crocetin enriching and purification is revealed.

The present invention relates to a composition derived from the hydrolysate of plant containing crocin or the derivatives thereof. The composition includes a significant amount of crocetin monoester. The present invention also provides a method to hydrolyze crocin existing in a plant extract, and further relates to the use.

The invention relates to a preparation method of the crocetin extracted from the gardenia, which is characterized in that after the gardenia is crushed and extracted by ultrasonic enhancement in distilled water or ethanol solution, the extractant is filtered and concentrated; the glycoside substance of the gardenia is separated via the macroporous resin and the crocin eluent is concentrated and dealt with acid hydrolysis or alkaline hydrolysis (alkaline hydrolysis solution should be adjusted to pH of acidity.), centrifugal, rinse and sedimentation to get the crude product of the crocetin; after purifying the crude product of the crocetin further, the cis crocetin and the trans crocetin are separated to acquire trans crocetin of high purity which facilitates structure modification, forms a plurality of crocetin derivatives featured by different water soluble and drug efficacy, providing material for related preparation R and D and application. The preparation method of the crocetin extracted from the gardenia has the advantages of having convenient and fast method, low cost and establishing a new channel for deep processing of gardenia products.

The invention discloses a method for preparing crocetin and crocin, which comprises the following steps: preparing a gardenia fruit extracting solution with ethanol under the conditions of ultrasonic waves, and concentrating the extracting solution to obtain an extract; and dissolving the extract in a polyethylene glycol aqueous solution, and selectively extracting with solvent to obtain crocetin and crocin. The invention does not need the chromatographic column separation process, has the advantages of simple operational method, and high product yield and purity, and is suitable for industrial production.

The invention provides a method for efficiently separating crocetin from fruits of rubiaceous plants and gardenia (stenophyllous gardenia, spoonleaf gardenia, gardenia hainanensis, gardenia sootepensis and gardenia) plants, saffron and saffron tissue cultures. The crocetin can be prepared by the method, and meanwhile, the gardenia yellow pigment and the saffron pigment can be prepared. The method comprises the following steps: extracting the pigment of gardenia fruits (the saffron and the saffron tissue cultures) through acid water or directly extracting raw materials through alkaline water, then carrying out water liquid acidification, then extracting ethyl acetate to obtain a crocetin crude product, and recrystallizing the crude product through different solvents to obtain a crocetin pure product. The crocetin, the gardenia yellow pigment and the saffron pigment provided by the invention can be applied in preparing cardiovascular and cerebrovascular disease medicaments, gynecological disease medicaments, tumor adjuvant therapy medicaments and foods. The method of the invention has reasonable design, the crocetin, the gardenia yellow pigment and the saffron pigment can be obtained in large scales and have high yield, and the method is quite suitable for industrialized production.

The invention relates to a method for preparing crocetin from jasmine, comprising the following steps: grinding the raw material jasmine, moistening the ground jasmine with acidulous water, adding bio-enzyme for enzymolysis at 30-40 DEG C for 3-5d, extracting the enzymolysis raw material with sodium hydroxide aqueous solution with pH value of 9-11, adjusting the pH value of the extract liquor to be neutral, adding anion exchange resins for adsorption, eluting the active ingredients with 0.1-1% of alkali liquor, adsorbing the eluent with macroporous resins after desalting the eluent with cation exchange resins, concentrating and crystallizing the eluent and recrystallizing ethanol. Used for producing crocetin, the method is low in cost and simple and easily operated in process. The method has high specificity.

The invention relates to the medicine field, and discloses a preparation method of crocetin. Extraction is carried out by adopting diluted alkali, extraction condensation and hydrolysis are combined together, and crocin is hydrolyzed and converted into crocetin during extraction and concentration. The preparation method comprises the steps of: crushing raw materials, carrying out conversion extraction by using a diluted alcoholic solution with pH of 10-14, regulating the pH to be 7-9 by using an extracting solution, condensing by decompression, filtering or centrifuging to remove sediment impurities, regulating the pH to be 2-5, filtering or centrifuging to collect red sediments and obtain a crocetin crude product with crocetin content higher than 50 percent, and recrystallizing and purifying the crocetin crude product to obtain the high-purity trans-crocetin with the crocetin content higher than 95 percent. The trans-crocetin can be used for preparing a medicament for treating fattyliver and has wide application prospect in prevention and control of tumors and other diseases.

The invention relates to unique compositions containing enriched and purified natural crocin and / or crocetin for prevention and / or treatment of cancers and other conditions and diseases. Compositions comprise mainly enriched or purified natural crocin or crocetin or combination of both and possible other active phytochemicals. A composition is used as functional food, drink, dietary supplement, or therapeutic dosage to a human orally or through other appropriate way (parenteral, percutaneous, rectal, mucosal, intranasal or topical administration). A method of natural crocin and crocetin enriching and purification is revealed.

The invention relates to a crocetin amides derivative. The crocetin amides derivative is characterized by having the following general formula: (img file='DSA00000789203800011.tif' wi='1009' he='177' / ), wherein R in the formula is (img file='DSA00000789203800012.tif' wi='1529' he='751' / ). The crocetin amides derivative has obvious inhibiting effect on human lung cancer cells A549 and human gastric carcinoma cells SGC7901. Therefore, the crocetin amides derivative can be applied to preparing anti-cancer drug. The invention also discloses a preparation method of the crocetin amides derivative.

The invention relates to the fields of biotechnology and comprehensive utilization of a natural product, and particularly relates to a method for integral preparation of a plurality of products with high additional value, such as crocetin, genipin, gardenia blue and the like by taking jasmine as a raw material through crushing, alcohol extraction and concentration without complicated separation means of resin, silica gel and the like by adopting simple chemical modification and biological catalysis technologies through a set of production technology. The purities of the prepared crocetin and genipin can be up to over 95%, and the color value E590 of the gardenia blue is greater than 110.

The invention relates to a synthetic method of crocetin, and belongs to the technical field of organic synthesis. By adapting 3, 7-dimethyl octa tr-ialkene bi-aldehyde and 2-bromopropionic acid methyl ester, the invention compounds out primary product of crocetin methyl ester after three steps of reactions. After hydrolysis, decolorization and recrystallization, the outgrowth in the reactions is effectively separated and purified after the recrystallization of the mixed solvent, in order to produce the product of the crocetin. The crocetin is one rare water soluble carotenoid acid in the nature extant in the saffron and gardenia. Moreover, the crocetin is the main component of the saffron, and has the functions of anticancer and tumor restricting property, blood fat reduction, liver protection, free radical destroy and oxidation resistance, and has the advantages of good water solubility, strong tinctorial strength, stable beam focusing, bright color of gold when the PH is within 1 to 4 and stable performance after pigmentation, so that the crocetin is widely used in the fields of medicine and food production. By simple chemical synthesis method, the invention produces the crocetin to release the demand for the saffron and satisfy the market needs.

The invention discloses a preparation method for high-purity trans-crocetin, which belongs to the field of extraction and separation of Chinese medicinal herbs. The method comprises the following specific steps of: transforming trans-crocetin in crude crocetin into trans-crocetin pyridinium, and crystalizing and recrystallizing through pyridine or a pyridine-containing solution to obtain trans-crocetin pyridinium; and making the trans-crocetin pyridinium react with an acid to obtain the high-purity trans-crocetin. The method disclosed by the invention has the advantage of easiness for repeating operation steps, controlling operation conditions, recovering crystalizing solvents and industrial amplifying in batch; and the purity of a final product, i.e., the trans-crocetin can be over 98 percent.

The invention relates to crocetin salt and an injection preparation thereof. The crocetin salt has good water solubility, can directly enter into blood, quickly acts and is stable and reliable. The injection of the crocetin salt can be prepared by dissolving the crocetin salt and sodium chloride into injection water. The injection is capable of improving the bioavailability of the crocetin salt and quickly taking treatment effect, and has stable quality.

The invention relates to a composition, and also relates to a Crocus sativus pigment active site extracted from Fructus Gardeniae. The composition mainly comprises trans-crocetin di(beta-D-gentiobiosyl)ester, trans-crocetin-beta-D-gentiobiosyl-beta-D-glucosyl ester, trans-crocetin di(beta-D-glucosyl)ester, 13-cis-crocetin di(beta-D-gentiobiosyl)ester, neocrocin B, trans-crocetin mono(beta-D-gentiobiosyl)ester, 13-cis-crocetin-8-O-beta-D-gentiobiosyl ester, 13-cis-crocetin-8'-O-beta-D-gentiobiosyl ester and trans-crocetin mono(beta-D-glucosyl)ester. Pharmacological experiment results show that the Crocus sativus pigment active site can effectively improve mouse learning memory injuries induced by scopolamine and amyloid beta protein.

The invention provides a method for synthesizing crocin glucoside, which comprises the following steps: a, synthesizing a C10 compound, namely 2,7-dimethyl-2,4,6-octatriene-1,8-dial (compound II); b, synthesizing a C5 compound, namely E-2-methyl-4-bromo-2- methyl crotonate; c, synthesizing crocetin dimethyl ester by taking the two compounds as raw materials; d, performing hydrolysis reaction on the crocetin dimethyl ester to obtain crocetin; and e, reacting the crocetin and 1,1'-carbonylbis-1h-imidazo to synthesize crocetin imidazole and further synthesize the crocin glucoside. The synthesizing process has the advantages in aspects of operation simplification and yield, and has high synthetic impurity, high yield, cost saving and strong industrial applicability.

The invention relates to a crocetin injection and a preparation method thereof. The crocetin contains crocetin and auxiliary materials which can help dissolve or regulate osmotic pressure. The weight ratio between the crocetin and the auxiliary materials is 1:10-1:500. The content of the crocetin raw material used in the injection involved in the present invention should be greater than 90%, and other impurities should be less than 10%. The preparation of the invention has good stability, low cost, all indexes meet the requirements of the preparation, and the preparation process has the characteristics of quick dissolution of raw materials and simple process, and is suitable for industrial production.

The invention provides a method for preparing crocetin from cape jasmine. The method comprises the following steps: extracting a cape jasmine raw material; performing enzymolysis on the raw material to obtain powder, and adding mixed enzyme; performing alkali extraction and enzymolysis, adding a reactant sodium hydroxide, stirring and extracting for 1 hour, filtering, separating a coarse product, and refiltering to obtain crocetin with content of over 50 percent; crystallizing to obtain crocetin, adding absolute ethyl alcohol, refluxing for 2 hours, cooling and crystallizing, filtering and baking to obtain the crocetin product with content of over 95 percent. The method can be used for solving the problems of drug shortage and high price of saffron.

The invention relates to the technical field of genetic engineering, and discloses a saffron crocus sourced CCD2 mutant, a coding sequence and application thereof, as well as a recombinant yeast strain for producing crocetin. The CCD2 mutant is provided with one or two or above of V120F, Y190K / A, R192V / F, E211A, E212A, T290V, K320A and S323F / A / T locus mutations. A rate-limiting enzyme CCD2 in thesynthesis route of the crocetin is transformed, the crocetin is directly synthesized from beta-carotene by widening the substrate spectrum of the CCD2, and the existing synthesis route of the crocetinis simplified and reconstructed, so that the problem that the total yield of multiple reactions is generally low is solved; and meanwhile, the efficiency of the CCD2 catalyzing an original substratezeaxanthin to synthesize the crocetin is improved, and the yeast strain of the high-yield crocetin is further obtained.

The invention discloses a method for preparing crocetin from fructus gardeniae. The method includes steps: 1) extraction, to be more specific, using 76.9% ethanol for extracting fructus gardeniae, combining extract liquid, concentrating the combined extract liquid, and performing spray drying to obtain powder for standby application; 2) enzymolysis, to be more specific, adding mixed enzymes into the powder obtained at the step 1) to realize enzymolysis under the condition that the temperature is 46-48DEG C, and pH is 5.6-6, wherein the mixed enzymes are obtained by mixing of plant extract complex enzymes SPE-005, plant extract complex enzymes SPE-007A and Aspergillus niger according to a proportion of 1:1:2.5; 3) alkaline extraction; 4) crystallization, to be more specific, adding 16-18 times of absolute ethyl alcohol into crocetin obtained at the step 3), refluxing for 2h at 78.9DEG C, cooling for crystallization, filtering and drying to obtain a crocetin product with crocetin content higher than 96%. According to the method, high-content crocetin can be extracted from the fructus gardeniae, and accordingly the problems of expensiveness and shortage of crocus sativus materials are solved.

The invention discloses a method for preparing high-purity crocetin with a gardenia yellow pigment. The method comprises the steps of extracting and separating the gardenia yellow pigment by silicagel column chromatography to form crocin with higher purity, hydrolyzing crocin under an alkaline condition to form crocetin with the higher purity. After the gardenia yellow pigment is separated and eluted by a chromatographic column, an eluant is collected and dried; a crocin content can reach above 90% through detection under 440nm wavelength of high efficiency liquid chromatography; after alkaline hydrolysis and N,N-dimethylformamide recrystallization, the purity of crocetin can reach above 95% through detection under 420nm wavelength of the high efficiency liquid chromatography; the prepared product is high in purity; the technology operability is strong; and the method can be widely applied to the field of medicines.

The invention relates to a preparation method of high purity crocetin, and belongs to the field of Chinese herbal medicine extraction separation. The concrete step of the method is as follows: dissolving crude crocetin in an alkaline solution, standing, crystallizing, and reacting crystal with an acid to obtain the high purity crocetin. The method has the advantages of easy-to-repeat steps, easy-to-control operation conditions, easy-to-process alkaline solution, easy industrialized batch amplification production, no organic solvent residue in the whole process, products capability of being used as food and drug additives, and the purity of the final product crocetin is detected by HPLC, and can reach above 98%.

The present invention relates to a composition derived from the hydrolysate of plant containing crocin or the derivatives thereof. The composition includes a significant amount of crocetin monoester. The present invention also provides a method to hydrolyze crocin existing in a plant extract, and further relates to the use.

The invention discloses a method for rapidly identifying vegetable dyes in dyed textile. According to the method, whether a test sample contains specific representative components in plant dyes or notis determined with HPLC (high performance liquid chromatography), and whether the test sample is dyed with corresponding plant dyes is identified. The plant dyes comprise at least one of madder, plant indigo blue, gardenia yellow and turmeric, and the specific representative components in the plant dyes specifically comprise alizarin and / or purpurin in the madder, curcumin in the turmeric, indirubin in the plant indigo blue and crocetin in the gardenia yellow. The same stripping solvent and sample preparation method is used, alizarin, purpurin, curcumin, indirubin and crocetin are determinedsimultaneously with HPLC, finally, the four plant dyes including the madder, the plant indigo blue, the gardenia yellow and the turmeric in dyed textile can be rapidly identified, and the method is rapid, accurate and efficient.

The invention discloses a method for preparing crocetin through gardenias. The method for preparing the crocetin through the gardenias comprises the steps of prewetting stockpiling, raw material treatment, first-time enzymolysis, deoiling, second-time enzymolysis, crocetin preparation, recrystallization and drying. According to the method for preparing the crocetin through the gardenias, vegetable oil and fat are extracted by adopting enzyme, oil components are separated from non-oil components by utilizing affinity differences of the non-oil components on oil and water and different proportions of the oil and the water, the production yield is increased, and the color value is increased.

The invention dislcoses application of crocetin in preparation of a drug or a health care product for preventing and treating polycystic ovarian syndrome. On the basis of the existing effects of the crocetin, a foetal period dihydrotestosterone-induced polycystic ovarian syndrome mouse model is adopted to deeply research therapeutic action of the crocetin. Results show that the crocetin can remarkably relieve estrous cycle dysrhythmia of a polycystic ovarian syndrome model mouse, reduce ovary weight and an atresia ovary amount, increasing amounts of corpora lutea big antral follicles and follicles before ovulation, and remarkably improve levels of estradiol, luteinizing hormone, follicle-stimulating hormone, progesterone and testosterone of the polycystic ovarian syndrome model mouse, andhas a good effect of treating the polycystic ovarian syndrome. Moreover, in an experiment process, obviously untoward effects do not appear, and the safety is high.

The invention belongs to the field of pharmaceutical preparations, and particularly relates to a preparation method of crocetin microemulsion and freeze-dried powder of the crocetin microemulsion. Crocetin has strong antioxidant activity, has effects on cardiovascular protection, skin cancer inhibition and the like, and has potential application value in medicine, health food and cosmetics. However, crocetin is insoluble in water and common organic solvents, so that use of crocetin is severely limited. Based on the microemulsification principle, the method for effectively increasing water solubility of crocetin is provided, solubility of crocetin in water is increased by 800 times, the problem that crocetin is insoluble in water is solved, and a foundation is laid for the application of crocetin.

The invention relates to the technical field of extraction and separation of natural plant active components, and particularly relates to a separation method of cis-trans isomerism crocetin. Crocetinbelongs to a carotenoid compound, has the effects of being strong in antioxidant activity, protecting heart and blood vessels and inhibiting skin cancer [1], and has potential application value in medicine and skin care products. Crocetin and glycoside thereof mainly exist in gardenia fruit and saffron. The gardenia fruit is low in value and rich in source can provide rich material source for crocetin, but has a cis-trans isomer. The invention provides a separation method of the cis-trans isomerism crocetin, by utilizing the difference of combining capacity of cis-trans isomerism crocetin withcalcium ions, all-trans-crocetin easily forms a regular molecule compound with Ca2+ ions, that is, all-trans-crocetin generates crystallization (precipitation) easily, while cis-crocetin cannot forma regular molecule compound with Ca2+ ions, that is, cis-crocetin does not form precipitation with Ca2+ easily, all trans-crocetin can be effectively separated from cis-crocetin.

A fraction separated from crude crocetin by preparative HPLC, and identified using LC / MS and NMR as crocetinic acid, markedly regressed the proliferation and increased apoptosis in pancreatic cancer cells. Purified crocetinic acid showed more potency than 15 commercial or crude crocetin using proliferation and apoptosis as markers. Purified crocetinic acid also showed significant anti-tumorigenic activity against pancreatic cancer cells in a mouse model of pancreatic cancer. Given crocetinic acid's low toxicity, crocetinic acid could be used as a chemotherapeutic or chemopreventative agent for pancreatic cancer.