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Method for extracting crocetin from gardenia fruit

A technology of crocetin and fruit, which is applied in the field of medicine, can solve the problems of low product purity, high price, and low yield, and achieve the effect of high product yield, low cost, and good purity

Inactive Publication Date: 2014-03-19
WEIHAI NOVODA PHARMA GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the problems of expensive raw materials used in the extraction and preparation of crocetin in the prior art, cumbersome process, low product purity,

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Crush 500g of gardenia fruit, use ultrasonic extraction technology, use 1500g of water as a solvent to extract for 2 hours, filter, repeat the above operations, combine the extracts, and concentrate under reduced pressure to a relative density of 1.15;

[0019] (2) Add 2000g of ethanol to the concentrated solution, place it under low temperature for 8 hours, filter off the precipitate, concentrate under reduced pressure, add 3000g of ethanol, place it under low temperature for 8 hours, remove the supernatant, and obtain the precipitate;

[0020] (3) Dissolve the above precipitate with an appropriate amount of water, inject it into HPD-300 macroporous resin column for adsorption, and elute with ethanol: water: glacial acetic acid volume ratio of 75: 25: 2, and concentrate under reduced pressure to relative The density is 1.15, and the concentrated solution is 22.5g by weighing;

[0021] (4) The above concentrated solution was put into 45g of saturated saline, crystal...

Embodiment 2

[0024] (1) Crush 300g of gardenia fruit, use ultrasonic extraction technology, use 1200g of water as a solvent to extract for 3 hours, filter, repeat the above operations, combine the extracts, and concentrate under reduced pressure to a relative density of 1.15;

[0025] (2) Add 1200g of ethanol to the concentrated solution, place it at low temperature for 7 hours, filter off the precipitate, concentrate under reduced pressure, add 1800g of ethanol, place it at low temperature for 7 hours, remove the supernatant, and obtain the precipitate;

[0026] (3) Dissolve the above precipitate with an appropriate amount of water, inject it into a HPD-300 macroporous resin column for adsorption, and elute with eluent with a volume ratio of ethanol: water: glacial acetic acid of 72: 25: 1.5, and concentrate under reduced pressure to relative The density is 1.15, and the concentrated solution is 16.2g by weighing;

[0027] (4) The above concentrated solution was put into 30 g of saturated...

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Abstract

The invention provides a method for extracting crocetin from a gardenia fruit. The method comprises the steps of ultrasonically extracting the crushed gardenia fruit for twice by adopting an ultrasonic extraction technology, concentrating the extracted liquid at reduced pressure, executing the alcohol precipitation under a specified condition, dissolving the precipitate in water, adding an HPD-300 porous resin column to adsorb, preparing the eluent with a given volume ratio through ethanol, water and glacial acetic acid, and refining the eluent through a saturated salt solution so as to obtain a relatively pure product. The product is detected through a high-efficiency liquid chromatogram, and the product content can reach more than 95 percent. The process is simple to operate, the cost is low, and the extraction rate is high.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to a method for extracting crocetin from gardenia fruit. Background technique [0002] The yield of saffron stigma is extremely low, and the resources are extremely limited, so it is difficult to cultivate it in a large area in China, which makes it expensive and known as "plant gold". The dried ripe fruit of the traditional Chinese medicine Gardenia is rich in yellow pigment, and its main components are crocin and crocetin. Crocetin is the aglycone of crocetin, which has an unsaturated conjugated polyenoic acid structure. A large number of studies have shown that crocetin is effective in anti-cardiovascular system diseases, anti-oxidation, anti-atherosclerosis, and prevention and treatment of diabetes. It has clear biological activity in terms of disease and other aspects, and has various physiological functions such as lowering cholesterol, inhibiting tumor growth, liver poiso...

Claims

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Application Information

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IPC IPC(8): C07C51/42C07C57/13
CPCC07C51/42C07C57/13
Inventor 王河东张庆卫赵艳
Owner WEIHAI NOVODA PHARMA GRP
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