Method for preparing crocetin and crocin

A technology for crocin and crocin, which is applied in chemical instruments and methods, preparation of sugar derivatives, separation/purification of carboxylic acid compounds, etc., can solve the problems of small processing capacity, unfavorable industrial production, etc. , easy to scale up and industrialized production, increase the effect of selectivity

Inactive Publication Date: 2012-05-02
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to overcome the disadvantages of small processing capacity and unfavorable industrial produc

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Take by weighing 500g of gardenia fruit coarse powder, ultrasonically extract four times, add 2500ml 95% ethanol to extract for 1.0 hour each time, combine four extracts and concentrate under reduced pressure to become extractum, extractum is dissolved in 200ml distilled water, and is continuously extracted with 200ml n-butanol Extract 3 times, combine n-butanol layers, concentrate under reduced pressure to form extract, take 2.2g extract and dissolve in 44ml water, add 0.88g polyethylene glycol-2000, extract with 44ml petroleum ether to remove small polar impurities, Then extracted 3 times with 44ml of ethyl acetate, combined the ethyl acetate layers, concentrated and dried under reduced pressure to obtain 185mg of crocetin, with a yield of 8.4%. The aqueous layer was precipitated with a mixed solution of ethyl acetate: ethanol = 3: 1 (V / V), filtered, the precipitate was dissolved in 100 ml of water, and extracted 3 times with a mixed solvent of 100 ml ethyl acetate: n-...

Embodiment 2

[0025] Weigh 500g of gardenia fruit powder, ultrasonically extract four times, add 5000ml 95% ethanol to extract for 1.5 hours each time, combine four extracts and concentrate under reduced pressure to form extractum, extractum is dissolved in 200ml distilled water, and is continuously extracted with 200ml n-butanol Extract 3 times, combine n-butanol layers, concentrate under reduced pressure to form extract, take 2.2g extract and dissolve in 55ml water, add 1.10g polyethylene glycol-2000, extract with 55ml petroleum ether to remove small polar impurities, Then extracted 3 times with 55ml of ethyl acetate, combined the ethyl acetate layers, concentrated and dried under reduced pressure to obtain 180mg of crocetin, with a yield of 8.2%. The aqueous layer was precipitated with ethyl acetate-ethanol 3:1 (V / V), filtered, the precipitate was dissolved in 100ml of water, extracted 3 times with 100ml of ethyl acetate:n-butanol=3:2 mixed solvent, the organic layers were combined, and t...

Embodiment 3

[0027] Take by weighing 500g of gardenia fruit coarse powder, ultrasonically extract three times, add 7500ml 95% ethanol to extract 1.0 hour each time, combine three extractions and concentrate under reduced pressure to become extractum, extractum is dissolved in 200ml distilled water, continuously extracts with 200ml n-butanol for 3 Once, the n-butanol layers were combined, concentrated under reduced pressure to form an extract, 2.2g of the extract was dissolved in 55ml of water, 1.10g of polyethylene glycol-2000 was added, and the impurities of small polarity were removed by extraction with 55ml of petroleum ether. 55ml of ethyl acetate was extracted 4 times, and the ethyl acetate layers were combined, concentrated and dried under reduced pressure to obtain 175mg of crocetin, with a yield of 8.0%. The aqueous layer was precipitated with ethyl acetate-ethanol 3:1 (V / V), filtered, the precipitate was dissolved in 100ml of water, extracted 3 times with 100ml of ethyl acetate:n-b...

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Abstract

The invention discloses a method for preparing crocetin and crocin, which comprises the following steps: preparing a gardenia fruit extracting solution with ethanol under the conditions of ultrasonic waves, and concentrating the extracting solution to obtain an extract; and dissolving the extract in a polyethylene glycol aqueous solution, and selectively extracting with solvent to obtain crocetin and crocin. The invention does not need the chromatographic column separation process, has the advantages of simple operational method, and high product yield and purity, and is suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of extracting active ingredients from plants. Specifically, it is a method for preparing crocetin and crocin. technical background [0002] Gardenia is an evergreen shrub of the Rubiaceae Gardenia genus. The dry and mature fruit of Gardenia is long oval or oval in shape, dark red or yellow red, and is one of the first batch of Chinese medicinal materials approved by the Ministry of Health of China. 1. It has the effects of treating colds, insomnia, icteric hepatitis, promoting blood circulation and removing blood stasis, etc. It is mainly produced in the hilly areas south of the Yangtze River. [0003] The water-soluble carotenoid pigment extracted and separated from the fruit of Gardenia jasminoides, its main component is crocin (also known as crocin, crocin, molecular formula C 44 h 64 o 24 ) and crocetin (also known as crocetin, crocetin, molecular formula C 20 h 24 o 4 ). [0004] Gardenia yellow...

Claims

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Application Information

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IPC IPC(8): C07C57/13C07C51/42C07H13/06C07H1/08C09B61/00
Inventor 韦万兴刘盛周敏凌秀荣
Owner GUANGXI UNIV
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