Method for preparing crocetin from fructus gardeniae
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A technology for saffron acid and gardenia, which is applied in the field of preparing saffron acid, can solve the problems of shortening the process, high price, shortage of saffron raw materials, etc., and achieves the effects of shortening the process, improving the yield, and improving the utilization rate of raw materials
Inactive Publication Date: 2016-08-31
CHENGDU YISHENGKE BIOTECH
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[0056] The purpose of the present invention is to design a method for preparing crocetin with gardenia as raw material, which can extract and prepare high-content crocetin from gardenia, which solves the shortage of saffron raw materials and the problem of high price. The method is directly extracted with water-containing alcohol Concentrate and spray dry, then convert crocetin into crocetin through enzymatic hydrolysis, and then extract crocetin with alkaline water, which reduces the pollution of organic reagents used in solv
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Embodiment 1
[0072] A method for preparing crocetin with Gardenia jasminoides as raw material, comprising the following processing steps:
[0073] 1) Extraction: Extract the gardenia raw material with 8-10 times the mass of 76.9% ethanol for 1-3 times, combine the extracts, concentrate the combined extracts, spray dry them, and powder them for later use;
[0074] 2) Enzymolysis: Add 6-7 times the amount of water and 1.2-1.4% mixed enzyme to the powder obtained in step 1), and enzymolyze it at 46-48°C and pH 5.6-6 for 24 hours; The mixed enzyme is mixed with plant extract compound enzyme SPE-005, plant extract compound enzyme SPE-007A and Aspergillus niger in a ratio of 1:1:2.5;
[0075] The plant extract compound enzyme SPE-005 mainly contains cellulase, pectinase, xylanase, and B-glucanase; the plant extract compound enzyme SPE-007A mainly contains B-glucoside Enzymes, including rhamnosidase, xylanase, B-glucanase, cellulase, hemicellulase;
[0076] 3) Alkaline extraction: After enzymat...
Embodiment 2
[0079] This embodiment is further optimized on the basis of the above-mentioned embodiments. In order to better realize the present invention, the following setting method is adopted in particular: In the step 1), 76.9% ethanol with 9 times the mass is used to extract twice, Combine the extracts.
Embodiment 3
[0081] This embodiment is further optimized on the basis of the above-mentioned embodiments. In order to better realize the present invention, the following setting method is adopted in particular: the dilute acid used in the step 3) is dilute hydrochloric acid.
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Abstract
The invention discloses a method for preparing crocetin from fructus gardeniae. The method includes steps: 1) extraction, to be more specific, using 76.9% ethanol for extracting fructus gardeniae, combining extract liquid, concentrating the combined extract liquid, and performing spray drying to obtain powder for standby application; 2) enzymolysis, to be more specific, adding mixed enzymes into the powder obtained at the step 1) to realize enzymolysis under the condition that the temperature is 46-48DEG C, and pH is 5.6-6, wherein the mixed enzymes are obtained by mixing of plant extract complex enzymes SPE-005, plant extract complex enzymes SPE-007A and Aspergillus niger according to a proportion of 1:1:2.5; 3) alkaline extraction; 4) crystallization, to be more specific, adding 16-18 times of absolute ethyl alcohol into crocetin obtained at the step 3), refluxing for 2h at 78.9DEG C, cooling for crystallization, filtering and drying to obtain a crocetin product with crocetin content higher than 96%. According to the method, high-content crocetin can be extracted from the fructus gardeniae, and accordingly the problems of expensiveness and shortage of crocus sativus materials are solved.
Description
technical field [0001] The invention relates to the technical field of biopharmaceuticals, in particular to a method for preparing crocetin from gardenia jasminoides. Background technique [0002] Gardenia (scientific name: Gardenia jasminoides Ellis) aliases: yellow gardenia, mountain gardenia, white toad, is the fruit of Rubiaceae gardenia. The fruit of gardenia is a traditional Chinese medicine, which belongs to the first batch of medicinal and edible resources promulgated by the Ministry of Health. In traditional Chinese medicine, it is often used to treat jaundice hepatitis, sprains, high blood pressure, diabetes and other diseases. [0003] Gardenia is a shrub, 0.3-3 meters high; shoots are often covered with short hairs, cylindrical, gray. Leaves opposite, leathery, rarely papery, at least 3 whorls, various leaf shapes, usually oblong-lanceolate, obovate-oblong, obovate or elliptic, 3-25 cm long, wide 1.5-8 cm, apex acuminate, suddenly long acuminate or short-point...
Claims
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