Method for preparing crocetin from fructus gardeniae

Abstract

The invention discloses a method for preparing crocetin from fructus gardeniae. The method includes steps: 1) extraction, to be more specific, using 76.9% ethanol for extracting fructus gardeniae, combining extract liquid, concentrating the combined extract liquid, and performing spray drying to obtain powder for standby application; 2) enzymolysis, to be more specific, adding mixed enzymes into the powder obtained at the step 1) to realize enzymolysis under the condition that the temperature is 46-48DEG C, and pH is 5.6-6, wherein the mixed enzymes are obtained by mixing of plant extract complex enzymes SPE-005, plant extract complex enzymes SPE-007A and Aspergillus niger according to a proportion of 1:1:2.5; 3) alkaline extraction; 4) crystallization, to be more specific, adding 16-18 times of absolute ethyl alcohol into crocetin obtained at the step 3), refluxing for 2h at 78.9DEG C, cooling for crystallization, filtering and drying to obtain a crocetin product with crocetin content higher than 96%. According to the method, high-content crocetin can be extracted from the fructus gardeniae, and accordingly the problems of expensiveness and shortage of crocus sativus materials are solved.

Description

technical field [0001] The invention relates to the technical field of biopharmaceuticals, in particular to a method for preparing crocetin from gardenia jasminoides. Background technique [0002] Gardenia (scientific name: Gardenia jasminoides Ellis) aliases: yellow gardenia, mountain gardenia, white toad, is the fruit of Rubiaceae gardenia. The fruit of gardenia is a traditional Chinese medicine, which belongs to the first batch of medicinal and edible resources promulgated by the Ministry of Health. In traditional Chinese medicine, it is often used to treat jaundice hepatitis, sprains, high blood pressure, diabetes and other diseases. [0003] Gardenia is a shrub, 0.3-3 meters high; shoots are often covered with short hairs, cylindrical, gray. Leaves opposite, leathery, rarely papery, at least 3 whorls, various leaf shapes, usually oblong-lanceolate, obovate-oblong, obovate or elliptic, 3-25 cm long, wide 1.5-8 cm, apex acuminate, suddenly long acuminate or short-point...

AI Technical Summary

Problems solved by technology

[0056] The purpose of the present invention is to design a method for preparing crocetin with gardenia as raw material, which can extract and prepare high-content crocetin from gardenia, which solves the shortage of saffron raw materials and the problem of high price. The method is directly extracted with water-containing alcohol Concentrate and spray dry, then convert crocetin into crocetin through enzymatic hydrolysis, and then extract crocetin with alkaline water, which reduces the pollution of organic reagents used in solvent extraction and is environmentally friendly; without using macroporous resin, the The usage of organic solvent is reduced, the process is shortened, and the cost is reduced; the most important thing is that all crocetin and crocin in gardenia raw materials can be extracted and converted into crocetin, which greatly improves the yield of crocetin and improves the utilization of raw materials High rate, able to extract saffron acid products with a content of more than 96%, which is beyond the reach of the existing technology