Method for preparing crocetin through gardenias

A technology of crocetin and gardenia, which is applied in the field of preparation of crocetin, can solve the problems of difficult large-scale cultivation, high price, limited resources, etc., and achieve the advantages of separation, improvement of extraction rate, and increase of product yield Effect

Inactive Publication Date: 2015-11-18
桂林三叶生物科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, saffron is only used as medicine on the stigma, and the yield is extremely low. Because of its extremely limited resources, it is

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method for preparing crocetin from gardenia, specifically comprising the steps of:

[0024] 1) Pre-wet stacking: build a pile of gardenias in a cool place, spray water, wait for the water to flow out from the bottom of the pile, and stack them naturally for 1 day;

[0025] 2) Raw material treatment: crush the gardenia obtained in step 1), add purified water equivalent to 2-3 times the weight of the raw material, heat to 50°C for 5 minutes, and obtain a paste-like slurry;

[0026] 3) Enzymolysis for the first time: the paste slurry obtained in step 2) is adjusted to pH 4, and 0.05% by weight of the paste slurry is added with an activity of 10,000 activity units / gram of pectin-cellulose compound enzyme and 0.001 wt. Part of mannitol was enzymolyzed for 40 minutes to obtain the first enzymolysis solution;

[0027] 4) Oil removal: add 2 times the weight of pure water to the first enzymolysis solution obtained in step 3), heat to 60°C and extract for 120 minutes to obtain...

Embodiment 2

[0032] A method for preparing crocetin from gardenia, specifically comprising the steps of:

[0033] 1) Pre-wet stacking: build a pile of gardenias in a cool place, spray water, wait for the water to flow out from the bottom of the pile, and stack them naturally for 1 day;

[0034] 2) Raw material processing: crush the gardenia obtained in step 1), add purified water equivalent to 2-3 times the weight of the raw material, heat to 60°C for 8 minutes, and obtain a paste-like slurry;

[0035] 3) Enzymolysis for the first time: adjust the pH of the paste slurry obtained in step 2) to 4.5, add 0.1% by weight of the paste slurry with an activity of 20,000 activity units / ml of pectin-cellulose compound enzyme and 0.001 wt. part of mannitol was enzymolyzed for 50 minutes to obtain the first enzymolysis solution;

[0036] 4) Oil removal: add 3 times the weight of pure water to the first enzymolysis solution obtained in step 3), heat to 70°C and extract for 60 minutes to obtain a mixtu...

Embodiment 3

[0041] 1) Pre-wet stacking: build a pile of gardenias in a cool place, spray water, and stack them naturally for 2 days after the water flows out from the bottom of the pile;

[0042] 2) Raw material treatment: crush the gardenia obtained in step 1), add purified water equivalent to 2-3 times the weight of the raw material, heat to 55°C for 10 minutes, and obtain a paste-like slurry;

[0043] 3) Enzymolysis for the first time: adjust the pH of the paste slurry obtained in step 2) to be 5, add 0.08% by weight of the paste slurry with an activity of 30,000 activity units / gram of pectin-cellulose compound enzyme and 0.001 wt. part of mannitol was enzymolyzed for 60 minutes to obtain the first enzymolysis solution;

[0044] 4) Oil removal: add 2 times the weight of pure water to the first enzymolysis solution obtained in step 3), heat to 65°C and extract for 100 minutes to obtain a mixture of the upper layer floating oil liquid, the lower layer liquid and the slag;

[0045] 5) Se...

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PUM

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Abstract

The invention discloses a method for preparing crocetin through gardenias. The method for preparing the crocetin through the gardenias comprises the steps of prewetting stockpiling, raw material treatment, first-time enzymolysis, deoiling, second-time enzymolysis, crocetin preparation, recrystallization and drying. According to the method for preparing the crocetin through the gardenias, vegetable oil and fat are extracted by adopting enzyme, oil components are separated from non-oil components by utilizing affinity differences of the non-oil components on oil and water and different proportions of the oil and the water, the production yield is increased, and the color value is increased.

Description

technical field [0001] The invention relates to an extraction method in the field of biotechnology, in particular to a method for preparing crocetin from Gardenia jasminoides. Background technique [0002] Gardenia jasminoides Ellis is the fruit of Rubiaceae (Gardenia jasminoides Ellis), oblong or oval, dark red or yellow-red. Gardenia jasminoides Ellis belongs to the first batch of medicinal and edible resources promulgated by the Ministry of Health, and is a traditional Chinese medicine. Fructus Sinensis has the effects of purging fire and relieving restlessness, clearing heat and promoting dampness, cooling blood and detoxifying, promoting gallbladder and protecting liver, lowering blood pressure, sterilizing and anti-inflammatory, and externally reducing swelling and relieving pain. Traditional Chinese medicine is mainly used to treat fever, jaundice hepatitis, sprains, high blood pressure, diabetes and so on. There are many chemical components in Fructus japonica, amon...

Claims

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Application Information

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IPC IPC(8): C12P7/44
Inventor 文继承
Owner 桂林三叶生物科技有限责任公司
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