The invention discloses a stable
microemulsion based on modified perilla
seed protein and a preparation method of the
microemulsion, and belongs to the technical field of
protein modification. The method comprises the following steps: adjusting the
moisture of perilla seed
meal, performing
extrusion pretreatment, then performing crushing and sieving, adding water, performing mixing to prepare a perilla seed
meal mixed solution, adjusting the pH value and temperature, adding
protease, carrying out enzymolysis, performing cooling to
room temperature, adjusting the pH value to neutrality, carrying out
ultrafiltration treatment to obtain perilla
seed protein with the molecular weight cutoff of 30-100kDa, and performing concentrating and
drying to obtain the perilla
seed protein with emulsifying activity; and uniformly mixing perilla seed
protein,
sucrose ester, flaxseed gum and polyglycerol ester, then adding flaxseed oil and a
preservative, and finally, performing high-speed shearing emulsification and high-pressure homogenization to obtain the stable
microemulsion. The preparation method is simple in production process, small in required
enzyme dosage and low in production cost, andthe prepared microemulsion is good in stability.