30results about How to "Facilitate subsequent enzymatic hydrolysis" patented technology

The invention discloses a production method of a low-salt oyster polypeptide and oligosaccharide nutrition powder. According to the production method, oyster obtained via shell removing is taken as a raw material, is repeatedly immersed in ice water at 4 to 10 DEG C for 2 to 4h; the oyster obtained via immersion desalting is subjected to slurry homogenizing, and is subjected to high temperature treatment for disinfection and protein denaturation; obtained oyster slurry is cooled with normal temperature water; pH value is adjusted to be 7.0 to 9.0, and 0.5 to 1.0wt% of protease is added for protein enzymatic hydrolysis; after high pH protease enzymatic hydrolysis, the temperature is adjusted, and 0.5 to 1% of a weakly acidic protease is added for enzymatic hydrolysis; amylase is added for degradation of glycogen; an obtained product is subjected to high temperature treatment, filtering, and spray drying so as to obtain the oyster polypeptide and oligosaccharide nutrition powder. The production method is simple; cost is low; and the obtained oyster polypeptide and oligosaccharide nutrition powder can be absorbed by human body easily.

The invention discloses preparation of a soybean anti-fatigue biological active peptide, belonging to an extraction processing technology of plant protein. The method comprises the following steps: adding water in high-denaturation soybean meal serving as a raw material to prepare a mixed solution; performing ultrasonic preprocessing on the mixed solution; adding neutral protease into the processed mixed solution to perform enzymolysis so as to obtain enzymatic hydrolysate; performing ultrafiltration treatment on the enzymatic hydrolysate by using a membrane separation technology after the enzymolysis to obtain biological active peptide with high peptide content and molecular weight of less than 5 kDa; then performing separation and purification by using anion exchange chromatography and reversed-phase high-performance liquid chromatography; and finally performing freeze drying to obtain the soybean anti-fatigue biological active peptide. The soybean anti-fatigue biological active peptide prepared by the preparation method disclosed by the invention has higher autioxidant enzyme activities and lower malondialdehyde (MDA) content after movement of a mouse; and results show that the soybean peptide has the functions of reducing cell injury caused by lipid oxidation to have an anti-fatigue effect by removing free radicals produced in the body and inhibiting the generation of lipid peroxides.

The invention provides a method for extracting peanut meal polysaccharide. The method comprises fives steps of pretreatment, protein enzymolysis, enzyme deactivation treatment, polysaccharide extraction from complex phosphoesterasum and separation purification, wherein complex phosphoesterasum is combination of cellulose, lysozyme, beta-glucanase and amylase. By adopting the method, protein in peanut meal polysaccharide can be effectively eliminated, the functional characteristics of polysaccharide is maintained to the maximum extent, contamination of organic solvents is avoided, the extraction rate of polysaccharide is improved, the purity of polysaccharide is improved, and a wide application prospect is achieved.

The invention discloses an anaerobic fermentation method for straw. The anaerobic fermentation method for the straw comprises the following steps that (1) sulfite is adopted to pretreat straw powder; (2) the pretreated straw powder is subjected to ultrasonic enzymolysis; (3) then the straw powder is soaked by using a sodium hydroxide solution; (4) the straw powder and the solution are separated, and acidogenic fermentation is conducted correspondingly; and (5) finally, methane-producing fermentation process is conducted. According to the anaerobic fermentation method for the straw, the yield of metabolite in the acid producing stage can be increased effectively so that the yield of methane and the use ratio of the straw can be increased.

The invention discloses a straw pretreatment method for cellulosic ethanol in straw. Pretreatment technologies for agricultural wastes such as the industrially produced straw mainly comprise acidic hydrolysis, alkaline hydrolysis, adding of chemicals for explosion and other pretreatment processes at the present, and have the problems of complicated treatment process, relatively high energy consumption and pollution to the environment to different extends. The straw pretreatment method for the cellulosic ethanol in the straw comprises the following steps: pre-soaking: removing sand from the straw through a sand remover to remove impurities; dewatering: pressurizing and heating a plug flow reactor through steam with pressure of 15 kg/cm<2> till the pressure is 13.5 kg/cm<2> and the temperature is 190 DEG C, retaining the pressure for 20 minutes, then discharging the straw, and carrying out two-stage flash distillation in a material discharging process of the plug flow reactor; gas production in the second stage of flash distillation during flash distillation: recycling steam from the first stage of flash distillation, producing gas from the second stage of flash distillation, cooling the gas through a cooler, and discharging the gas into a sewage treatment device. The straw pretreatment method is applied to the field of bio-refinery.

The invention discloses a method for improving the perilla seed protein emulsifying activity, and belongs to the technical field of perilla seed protein modification. The method comprises the following steps that 1, perilla seed meal is subjected to water conditioning, and after water conditioning, ultrahigh-pressure pretreatment is conducted; 2, the perilla seed meal obtained after ultrahigh-pressure pretreatment is smashed, screened and mixed by adding water to prepare a 4-20% mixed liquid, pH and temperature are adjusted, enzymolysis is conducted by adding protease, after enzymolysis, temperature is lowered to the room temperature, and the pH value is adjusted to be neutral; 3, the enzymolysis liquid is subjected to ultrafiltration, perilla seed protein with the molecular weight cut-offof 5-30 kDais obtained, the perilla seed protein is subjected to concentration and spray drying, and the perilla seed protein high in emulsifying activity is prepared. The method has the advantages that the production process is simple, the required enzyme using amount is low, the enzymolysis time is short, and the production cost is low.

The invention belongs to the field of protein peptide preparation, and relates to a preparation method of abalone viscera protein peptide, which comprises the following steps: pretreating abalone viscera with saturated salt water, adding water into the pretreated abalone viscera, and crushing; adding a compound enzyme I prepared from pancreatin, chymotrypsin, papain, carboxypeptidase and alkyl mercaptan according to the mass ratio of 1: (0.1-0.2): (2-5): (0.5-1.5): (0.01-0.1) to carry out primary enzymolysis, and adding a compound enzyme II prepared from papain, carboxypeptidase and sodium salt according to the mass ratio of 1: (0.05-0.2): (0.01-0.1) to carry out secondary enzymolysis, and adding a flocculating agent into the obtained enzymatic hydrolysate, carrying out flocculent precipitation by using the flocculating agent containing alginate, gelatin, carrageenan and modified aluminum hydroxide, and carrying out solid-liquid separation. The method provided by the invention can improve the yield and purity of abalone viscera protein peptide, and has great industrial application prospects.