Patents
Literature
Hiro is an intelligent assistant for R&D personnel, combined with Patent DNA, to facilitate innovative research.
Hiro

30results about How to "Facilitate subsequent enzymatic hydrolysis" patented technology

Preparation method of egg albumen powder with great instant property and no bitter and fishy smells

The invention discloses a preparation method of egg albumen powder with a great instant property and no bitter or fishy smells, which solves the problems of bad instant property, bitter smell or fishy smell, and bad organoleptic quality of egg albumen powder in the market at present. According to the preparation method, enzymolysis treatment is performed on egg albumen protein by a compound protease (including Alcalase, neutral protease and flavourzyme) and cooperates with a two-stage enzymolysis method, the use amount of the compound protease is less than the use amount of any one protease in the compound protease during independent use, the physicochemical indexes of instant property, taste and the like of the egg albumen powder obtained via the enzymolysis are greatly improved, and unexpected technical effects are obtained; the proportioning ratio of Alcalase to neutral protease to flavourzyme in the compound protease is determined to be 1: 2: 1, and via the proportioning, the instant property and dissolution stability of the egg albumen powder are remarkably improved; and high-speed short-time superfine treatment is combined, so that the effects are better.
Owner:迟玉杰

Production method of low-salt oyster polypeptide and oligosaccharide nutrition powder

The invention discloses a production method of a low-salt oyster polypeptide and oligosaccharide nutrition powder. According to the production method, oyster obtained via shell removing is taken as a raw material, is repeatedly immersed in ice water at 4 to 10 DEG C for 2 to 4h; the oyster obtained via immersion desalting is subjected to slurry homogenizing, and is subjected to high temperature treatment for disinfection and protein denaturation; obtained oyster slurry is cooled with normal temperature water; pH value is adjusted to be 7.0 to 9.0, and 0.5 to 1.0wt% of protease is added for protein enzymatic hydrolysis; after high pH protease enzymatic hydrolysis, the temperature is adjusted, and 0.5 to 1% of a weakly acidic protease is added for enzymatic hydrolysis; amylase is added for degradation of glycogen; an obtained product is subjected to high temperature treatment, filtering, and spray drying so as to obtain the oyster polypeptide and oligosaccharide nutrition powder. The production method is simple; cost is low; and the obtained oyster polypeptide and oligosaccharide nutrition powder can be absorbed by human body easily.
Owner:荣成市海洋绿洲生物科技有限公司

Preparation of soybean anti-fatigue biological active peptide

The invention discloses preparation of a soybean anti-fatigue biological active peptide, belonging to an extraction processing technology of plant protein. The method comprises the following steps: adding water in high-denaturation soybean meal serving as a raw material to prepare a mixed solution; performing ultrasonic preprocessing on the mixed solution; adding neutral protease into the processed mixed solution to perform enzymolysis so as to obtain enzymatic hydrolysate; performing ultrafiltration treatment on the enzymatic hydrolysate by using a membrane separation technology after the enzymolysis to obtain biological active peptide with high peptide content and molecular weight of less than 5 kDa; then performing separation and purification by using anion exchange chromatography and reversed-phase high-performance liquid chromatography; and finally performing freeze drying to obtain the soybean anti-fatigue biological active peptide. The soybean anti-fatigue biological active peptide prepared by the preparation method disclosed by the invention has higher autioxidant enzyme activities and lower malondialdehyde (MDA) content after movement of a mouse; and results show that the soybean peptide has the functions of reducing cell injury caused by lipid oxidation to have an anti-fatigue effect by removing free radicals produced in the body and inhibiting the generation of lipid peroxides.
Owner:NORTHEAST AGRICULTURAL UNIVERSITY

Method for extracting peanut meal polysaccharide

The invention provides a method for extracting peanut meal polysaccharide. The method comprises fives steps of pretreatment, protein enzymolysis, enzyme deactivation treatment, polysaccharide extraction from complex phosphoesterasum and separation purification, wherein complex phosphoesterasum is combination of cellulose, lysozyme, beta-glucanase and amylase. By adopting the method, protein in peanut meal polysaccharide can be effectively eliminated, the functional characteristics of polysaccharide is maintained to the maximum extent, contamination of organic solvents is avoided, the extraction rate of polysaccharide is improved, the purity of polysaccharide is improved, and a wide application prospect is achieved.
Owner:山东金胜生物科技有限公司

Method for preparing sesame oil by solid state fermentation aqueous enzymatic method

The invention discloses a method for preparing sesame oil by a solid state fermentation aqueous enzymatic method and belongs to the field of plant oil extraction and processing. The method comprises the following steps of 1, adjusting moisture of hulled sesame, carrying out autoclaved sterilization, cooling the sterile sesame, putting the cooled sesame into a solid state fermentation tank, and carrying out solid state fermentation to obtain fermentation products, and 2, crushing the fermentation products obtained by the step 1, mixing the crushed fermentation products and water, adding protease into the mixture, carrying out enzymatic hydrolysis, carrying out alkaline extraction, carrying out centrifugalization to obtain free oil, an emulsion, a hydrolysate and residues, carrying out demulsification of the emulsion and the hydrolysate, and merging the free oil, and the emulsion and the hydrolysate subjected by demulsification to obtain the sesame oil. The method realizes sesame oil preparation by combination of solid state fermentation and the biological enzyme, adopts simple equipment, is safe in operation, has a high oil yield, can produce the sesame oil having good quality and a high nutrition value, can produce a high-activity sesame protein and a sesame peptide under the action of the biological compound enzyme, and can reduce protein resource waste.
Owner:NORTHEAST AGRICULTURAL UNIVERSITY

Anaerobic fermentation method for straw

The invention discloses an anaerobic fermentation method for straw. The anaerobic fermentation method for the straw comprises the following steps that (1) sulfite is adopted to pretreat straw powder; (2) the pretreated straw powder is subjected to ultrasonic enzymolysis; (3) then the straw powder is soaked by using a sodium hydroxide solution; (4) the straw powder and the solution are separated, and acidogenic fermentation is conducted correspondingly; and (5) finally, methane-producing fermentation process is conducted. According to the anaerobic fermentation method for the straw, the yield of metabolite in the acid producing stage can be increased effectively so that the yield of methane and the use ratio of the straw can be increased.
Owner:BIOGAS SCI RES INST MIN OF AGRI

Stable microemulsion based on modified perilla seed protein and preparation method of microemulsion

The invention discloses a stable microemulsion based on modified perilla seed protein and a preparation method of the microemulsion, and belongs to the technical field of protein modification. The method comprises the following steps: adjusting the moisture of perilla seed meal, performing extrusion pretreatment, then performing crushing and sieving, adding water, performing mixing to prepare a perilla seed meal mixed solution, adjusting the pH value and temperature, adding protease, carrying out enzymolysis, performing cooling to room temperature, adjusting the pH value to neutrality, carrying out ultrafiltration treatment to obtain perilla seed protein with the molecular weight cutoff of 30-100kDa, and performing concentrating and drying to obtain the perilla seed protein with emulsifying activity; and uniformly mixing perilla seed protein, sucrose ester, flaxseed gum and polyglycerol ester, then adding flaxseed oil and a preservative, and finally, performing high-speed shearing emulsification and high-pressure homogenization to obtain the stable microemulsion. The preparation method is simple in production process, small in required enzyme dosage and low in production cost, andthe prepared microemulsion is good in stability.
Owner:SHAANXI UNIV OF SCI & TECH

Straw pretreatment method for cellulosic ethanol in straw

InactiveCN106834362AGuarantee hydrolysisGuarantee the fermentation environmentBiofuelsFermentationPretreatment methodHigh energy
The invention discloses a straw pretreatment method for cellulosic ethanol in straw. Pretreatment technologies for agricultural wastes such as the industrially produced straw mainly comprise acidic hydrolysis, alkaline hydrolysis, adding of chemicals for explosion and other pretreatment processes at the present, and have the problems of complicated treatment process, relatively high energy consumption and pollution to the environment to different extends. The straw pretreatment method for the cellulosic ethanol in the straw comprises the following steps: pre-soaking: removing sand from the straw through a sand remover to remove impurities; dewatering: pressurizing and heating a plug flow reactor through steam with pressure of 15 kg / cm<2> till the pressure is 13.5 kg / cm<2> and the temperature is 190 DEG C, retaining the pressure for 20 minutes, then discharging the straw, and carrying out two-stage flash distillation in a material discharging process of the plug flow reactor; gas production in the second stage of flash distillation during flash distillation: recycling steam from the first stage of flash distillation, producing gas from the second stage of flash distillation, cooling the gas through a cooler, and discharging the gas into a sewage treatment device. The straw pretreatment method is applied to the field of bio-refinery.
Owner:黑龙江中丹建业生物能源有限公司

Method for improving perilla seed protein emulsifying activity

The invention discloses a method for improving the perilla seed protein emulsifying activity, and belongs to the technical field of perilla seed protein modification. The method comprises the following steps that 1, perilla seed meal is subjected to water conditioning, and after water conditioning, ultrahigh-pressure pretreatment is conducted; 2, the perilla seed meal obtained after ultrahigh-pressure pretreatment is smashed, screened and mixed by adding water to prepare a 4-20% mixed liquid, pH and temperature are adjusted, enzymolysis is conducted by adding protease, after enzymolysis, temperature is lowered to the room temperature, and the pH value is adjusted to be neutral; 3, the enzymolysis liquid is subjected to ultrafiltration, perilla seed protein with the molecular weight cut-offof 5-30 kDais obtained, the perilla seed protein is subjected to concentration and spray drying, and the perilla seed protein high in emulsifying activity is prepared. The method has the advantages that the production process is simple, the required enzyme using amount is low, the enzymolysis time is short, and the production cost is low.
Owner:SHAANXI UNIV OF SCI & TECH

A method for extracting huperzine A from Melaleuca

The invention discloses a method for extracting huperzine A from huperzia serrata. The method comprises the steps of soaking treatment, raw material treatment, enzymolysis, deoiling, acidolysis, column chromatography and recrystallization. According to the method for extracting the huperzine A from the huperzia serrata, vegetable oil and fat are extracted by adopting enzyme, oil components are separated from non-oil components by utilizing affinity differences of the non-oil components on oil and water and different proportions of the oil and the water, the production yield is increased, and the extraction rate is increased.
Owner:桂林三宝生物科技有限公司

Method for extracting geniposide from folium cortex eucommiae

The invention discloses a method for extracting geniposide from folium cortex eucommiae. The method comprises the following steps of pre-wetting stacking, raw material treating, first-time enzymolysis, deoiling, second-time enzymolysis, column chromatography and drying. According to the method, firstly, vegetable fat is extracted through enzyme, oil ingredients and non-oil ingredients are separated based on the affinity difference of the non-oil ingredients to oil and water and the different specific gravities of oil and water, and the product yield and the extracting rate are increased.
Owner:广西优食客食品有限公司

Method for extracting geniposide from gardenia jasminoides ellis

The invention discloses a method for extracting geniposide from gardenia jasminoides ellis. The method comprises steps as follows: prewetting, stacking, raw material processing, first enzymolysis, deoiling, second enzymolysis, column chromatography and drying. According to the method, vegetable oil is extracted with enzyme, oil ingredients and non-oil ingredients are separated according to affinity difference of the non-oil ingredients to oil and water and different specific gravities of oil and water, the yield of products is increased, and the extraction rate is increased.
Owner:广西优食客食品有限公司

Preparation method of abalone viscera protein peptide

The invention belongs to the field of protein peptide preparation, and relates to a preparation method of abalone viscera protein peptide, which comprises the following steps: pretreating abalone viscera with saturated salt water, adding water into the pretreated abalone viscera, and crushing; adding a compound enzyme I prepared from pancreatin, chymotrypsin, papain, carboxypeptidase and alkyl mercaptan according to the mass ratio of 1: (0.1-0.2): (2-5): (0.5-1.5): (0.01-0.1) to carry out primary enzymolysis, and adding a compound enzyme II prepared from papain, carboxypeptidase and sodium salt according to the mass ratio of 1: (0.05-0.2): (0.01-0.1) to carry out secondary enzymolysis, and adding a flocculating agent into the obtained enzymatic hydrolysate, carrying out flocculent precipitation by using the flocculating agent containing alginate, gelatin, carrageenan and modified aluminum hydroxide, and carrying out solid-liquid separation. The method provided by the invention can improve the yield and purity of abalone viscera protein peptide, and has great industrial application prospects.
Owner:韩耀辉

A method for extracting luteolin from peanut shells

The invention discloses a method for extracting luteolin from peanut shells. The method comprises the following steps of steeping treatment, raw material treatment, enzymolysis, deoiling, alkaline extraction, extraction and recrystallization. According to the method, vegetable fat is extracted through enzyme, oil ingredients and non-oil ingredients are separated based on the affinity difference of the non-oil ingredients to oil and water and the different specific gravities of oil and water, and the product yield and the extracting rate are increased.
Owner:桂林三宝生物科技有限公司

The method of extracting genipin from gardenia

The invention discloses a method for extracting genipin from gardenias. The method includes the following steps of pre-wetting stacking, raw material processing, first time of the enzymolysis, de-oiling, second time of enzymolysis, column chromatography, third time of enzymolysis, column chromatography and drying. According to the method, vegetable oil and fat are firstly extracted through enzyme, oil and non-oil components are separated through affinity differences of the non-oil components on oil and water, and different oil and water proportions, and the product yield and the conversation rate are increased.
Owner:广西优食客食品有限公司

A method for extracting geniposide from Eucommia leaves

The invention discloses a method for extracting geniposide from folium cortex eucommiae. The method comprises the following steps of pre-wetting stacking, raw material treating, first-time enzymolysis, deoiling, second-time enzymolysis, column chromatography and drying. According to the method, firstly, vegetable fat is extracted through enzyme, oil ingredients and non-oil ingredients are separated based on the affinity difference of the non-oil ingredients to oil and water and the different specific gravities of oil and water, and the product yield and the extracting rate are increased.
Owner:广西优食客食品有限公司

Method for extracting geniposide acid from folium cortex eucommiae

The invention discloses a method for extracting geniposide acid from folium cortex eucommiae. The method comprises the following steps of pre-wetting stacking, raw material treating, first-time enzymolysis, deoiling, second-time enzymolysis, column chromatography and drying. According to the method, firstly, vegetable fat is extracted through enzyme, oil ingredients and non-oil ingredients are separated based on the affinity difference of the non-oil ingredients to oil and water and the different specific gravities of oil and water, and the product yield and the extracting rate are increased.
Owner:广西优食客食品有限公司

Soybean ACE inhibitory peptide and preparation method and application thereof

The invention discloses a soybean ACE inhibitory peptide and a preparation method and application thereof. The method comprises the following steps that 1 part by weight of soybean protein isolate and3-5 parts by weight of an alkaline solution are taken, mixed to be uniform and ground into thick liquid, the thick liquid is heated, alkaline protease is added for enzymolysis, the pH value is adjusted, neutral protease is added for hydrolysis, and soybean enzymatic hydrolysate is obtained; and tea polyphenol and diatomite are addded, filtering, and spray drying to is performed obtain the soybeanACE inhibitory peptide. The soybean protein isolate is pretreated by hot alkali so that on one hand, a hydration layer on the surface of the soybean protein can be destroyed, hydrophobic groups in the protein are exposed, the protease cleavage site is increased, and subsequent enzymolysis is facilitated; in addition, part of soybean protein can be hydrolyzed to release polypeptide functional groups with amphipathy so that the pH value of a protein solution is reduced, and the effect of protease is facilitated; more hydrophobic groups are exposed, the protein is degraded, the viscosity of a protein aqueous solution is obviously reduced, and the contact probability of protease and the protein is increased.
Owner:广东健明生物科技有限公司

A kind of method extracting geniposide from gardenia

The invention discloses a method for extracting geniposide from gardenia jasminoides ellis. The method comprises steps as follows: prewetting, stacking, raw material processing, first enzymolysis, deoiling, second enzymolysis, column chromatography and drying. According to the method, vegetable oil is extracted with enzyme, oil ingredients and non-oil ingredients are separated according to affinity difference of the non-oil ingredients to oil and water and different specific gravities of oil and water, the yield of products is increased, and the extraction rate is increased.
Owner:广西优食客食品有限公司

A method for preparing crocetin from gardenia

The invention discloses a method for preparing crocetin through gardenias. The method for preparing the crocetin through the gardenias comprises the steps of prewetting stockpiling, raw material treatment, first-time enzymolysis, deoiling, second-time enzymolysis, crocetin preparation, recrystallization and drying. According to the method for preparing the crocetin through the gardenias, vegetable oil and fat are extracted by adopting enzyme, oil components are separated from non-oil components by utilizing affinity differences of the non-oil components on oil and water and different proportions of the oil and the water, the production yield is increased, and the color value is increased.
Owner:桂林三叶生物科技有限责任公司

Method for extracting huperzine A from huperzia serrata

The invention discloses a method for extracting huperzine A from huperzia serrata. The method comprises the steps of soaking treatment, raw material treatment, enzymolysis, deoiling, acidolysis, column chromatography and recrystallization. According to the method for extracting the huperzine A from the huperzia serrata, vegetable oil and fat are extracted by adopting enzyme, oil components are separated from non-oil components by utilizing affinity differences of the non-oil components on oil and water and different proportions of the oil and the water, the production yield is increased, and the extraction rate is increased.
Owner:桂林三宝生物科技有限公司

A kind of anaerobic fermentation method of straw

The invention discloses an anaerobic fermentation method for straw. The anaerobic fermentation method for the straw comprises the following steps that (1) sulfite is adopted to pretreat straw powder; (2) the pretreated straw powder is subjected to ultrasonic enzymolysis; (3) then the straw powder is soaked by using a sodium hydroxide solution; (4) the straw powder and the solution are separated, and acidogenic fermentation is conducted correspondingly; and (5) finally, methane-producing fermentation process is conducted. According to the anaerobic fermentation method for the straw, the yield of metabolite in the acid producing stage can be increased effectively so that the yield of methane and the use ratio of the straw can be increased.
Owner:BIOGAS SCI RES INST MIN OF AGRI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products